Protein Nitrogen Content and Phosphorylative Activity of Apple Fruits During Ripening and Senescence

1965 ◽  
Vol 18 (6) ◽  
pp. 1093 ◽  
Author(s):  
TL Lewis ◽  
D Martin
Keyword(s):  
Nitrogen Content ◽  
Respiratory Activity ◽  
Protein Nitrogen ◽  
Apple Varieties ◽  
Apple Fruits

Respiratory activity, protein nitrogen content, and rate of esterification of infiltrated 32p have been followed during ripening and senescence at 20�0 in detached fruits of five apple varieties.

scholarly journals The Physiology of Growth in Apple Fruits III. Cell Characteristics and Respiratory Activity of Light and Heavy Crop Fruits

1952 ◽  
Vol 5 (3) ◽  
pp. 315 ◽  
Author(s):  
D Martin ◽  
TL Lewis
Keyword(s):  
Cell Volume ◽  
Cell Size ◽  
Respiratory Activity ◽  
Cell Number ◽  
Cell Protein ◽  
Protein Nitrogen ◽  
Apple Varieties ◽  
Apple Fruits

Cell size, total and protein nitrogen, and preclimacteric respiration have been studied for light and heavy crop fruit of certain Tasmanian-grown apple varieties. Differences in size of fruit from light and heavy crops have been shown to be due mainly to differences in cell size rather than in cell number. Respiration per cell, protein nitrogen per cell, and cell volume were closely intercorrelated but respiration per unit protein is greater in light crop fruit than in heavy crop.

Direct and Indirect Determination of True Protein Content of Milk by Kjeldahl Analysis: Collaborative Study

1991 ◽  
Vol 74 (2) ◽  
pp. 281-288 ◽  
Author(s):  
David M Barbano ◽  
Joanna M Lynch ◽  
J Richard Fleming
Keyword(s):  
Nitrogen Content ◽  
Protein Content ◽  
Total Nitrogen ◽  
Collaborative Study ◽  
Total Nitrogen Content ◽  
Nonprotein Nitrogen ◽  
Protein Nitrogen ◽  
Nitrogen Determination ◽  
True Protein

Abstract Currently, the reference procedure for determination of the "protein" content of milk is based on measurement of the total nitrogen content of milk by the Kjeldahl method (AOAC method, 920.105). About 6% of the total nitrogen content of milk Is nonprotein nitrogen. Therefore, total nitrogen multiplied by the conversion factor 6.38 overestimates the true protein content of milk on average by about 6%. In the present study, new direct and Indirect methods were developed for measurement of the true protein content of whole milk by Kjeldahl nitrogen determination. Both new methods are sample preparation procedures used to fractionate the nitrogen-containing compounds In milk prior to measurement of the nitrogen content of these fractions by Kjeldahl analysis. The collaborative study consisted of 9 pairs of blind duplicate milk samples that were analyzed for total nitrogen, nonprotein nitrogen, and protein nitrogen by each of 10 laboratories. Both methods for true protein measurement (direct and Indirect) gave acceptable statistical performance characteristics and good agreement between methods. The new direct method requires about half the laboratory analysis work of the indirect method (i.e., total minus nonprotein nitrogen). The methods have been adopted official first action by AOAC as (1) a new method for nonprotein nitrogen determination in milk, (2) a new method (direct) for determination of protein nitrogen content of milk, and {3) an alternative method (indirect) for determination of protein nitrogen content of milk.

Variation of Total Nitrogen, Non-protein Nitrogen Content, and Types of Alkaloids at Different Stages of Development inErythrina americanaSeeds†

1996 ◽  
Vol 44 (10) ◽  
pp. 2987-2991 ◽  
Author(s):  
R. García-Mateos ◽  
B. Lucas ◽  
M. Zendejas ◽  
M. Soto-Hernández ◽  
M. Martínez ◽  
...  
Keyword(s):  
Nitrogen Content ◽  
Total Nitrogen ◽  
Stages Of Development ◽  
Protein Nitrogen

Biochemical changes in progressive muscular dystrophy. VII. Studies on the biosynthesis of protein and RNA in various cellular fractions of the muscle of normal and dystrophic mice

1968 ◽  
Vol 46 (1) ◽  
pp. 35-41 ◽  
Author(s):  
Uma Srivastava
Keyword(s):  
Nitrogen Content ◽  
Microsomal Protein ◽  
Dystrophic Muscle ◽  
Protein Nitrogen ◽  
Progressive Muscular Dystrophy ◽  
Rna Content ◽  
Disease Protein ◽  
Dystrophic Mice ◽  
Muscle Homogenate

Protein nitrogen content, RNA concentration, and in vivo incorporation of L-[U-14C]leucine into protein and of [2-14C]uridine into RNA of homogenate and various fractions of muscle of normal and dystrophic mice were measured at various stages of the disease. Protein nitrogen content was always lower in dystrophic than in normal muscle, and this became more pronounced with the progress of the disease. Most of the decrease was due to loss of proteins from the myofibrils. RNA content increased in the homogenate, nuclei–myofibrils, supernatant, and microsomes of dystrophic muscle. In the mitochondria of dystrophic muscle, no change was noted compared to controls. The ratio of RNA content to protein in the homogenate, nuclei–myofibrils, supernatant, and microsomes was also greater in dystrophic muscle. It was not changed in dystrophic muscle mitochondria. Incorporation of L-[U-14C]leucine into proteins of dystrophic muscle homogenate and various fractions also increased to variable degrees over that in the controls. It was further observed that mitochondrial and microsomal protein incorporate L-[U-14C]leucine in dystrophic muscle at an increased rate but the disappearance of 14C was even greater, compared to controls.In vivo incorporation of [2-14C]uridine into RNA of dystrophic muscle increased at 30 days', remained the same at 60 days', and declined at 90 days' duration of the disease. Similar results were also obtained in the nuclei–myofibrillar fraction of dystrophic muscle. In all other fractions an increase was noted in incorporation in dystrophic muscle. The incorporation of [2-14C]uridine into RNA in supernatant and microsomes was higher in dystrophic muscle but the disappearance of 14C was greater, compared to controls. It is quite evident in the microsomal fraction at 90 days, where no change in the incorporation is noted in normal and dystrophic animals.

scholarly journals Biochemical composition of scab-immune apple fruits varieties (Malus domestica B.) as a valuable component of healthy dietary

2021 ◽  
Vol 30 ◽  
pp. 01018
Author(s):  
Natalia Saveleva ◽  
Nadezhda Borzykh ◽  
Vladislav Chivilev ◽  
Andrey Yushkov ◽  
Aleksander Zemisov ◽  
...  
Keyword(s):  
Malus Domestica ◽  
Biochemical Composition ◽  
Food Production ◽  
Soluble Solids ◽  
Valuable Component ◽  
Apple Varieties ◽  
Apple Fruits ◽  
Long Term Studies

The article presents an analysis of the biochemical composition of apple varieties (Malus domestica B.) with immunity to scab. Domestic and foreign apple varieties are used as research objects. Variability in fruits in the content of ascorbic acid (AA), soluble solids (SDS), P-active compounds, and titratable acids was noted. The high content of AA is noted in Uspenskoe, Skala, Bylina, Flagman, Yubilyar apple varieties. Long-term studies have revealed intervarietal differences in biochemical composition and variation of this trait over the years in apples. Varieties with valuable biochemical composition and immunity to scab can be used in organic food production, as well as in further breeding work to improve the quality of fruits.

scholarly journals Protein fractions of oats and possibilities of oat utilisation for patients with coeliac disease

2011 ◽  
Vol 22 (No. 4) ◽  
pp. 151-162 ◽  
Author(s):  
I. Capouchová ◽  
J. Petr ◽  
H. Tlaskalová-Hogenová ◽  
I. Michalík ◽  
O. Faměra ◽  
...  
Keyword(s):  
Coeliac Disease ◽  
Nitrogen Content ◽  
Protein Complex ◽  
Total Protein ◽  
Electrophoretic Analysis ◽  
Gluten Free Diet ◽  
Protein Fractions ◽  
Gluten Free ◽  
Protein Nitrogen ◽  
Average Percentage

The applicability was evaluated of 16 different oats species and varieties of different provenance in the coeliac diet in view of the composition of the protein complex and immunological testing during two-year experiments (2001 and 2002). Determination was carried out of total nitrogen content (average of evaluated oats collection in 2001 was 2.21%, in 2002 2.78%), protein nitrogen content (average 2001 1.94%, 2002 2.28%), and crude protein (N × 6.25) content (average 2001 13.80%, 2002 17.37%). The proportions of different protein fractions play a decisive role for the aims of this study because, based on the existing knowledge, coeliacally active protein components are present particularly in the prolamin fraction. The percentage of prolamins (determined by discontinual fractionation after Osborne) in the author’s evaluated collection of oats species and varieties under the conditions of Central Bohemia reached on average 17.68% of the total protein in 2001, and 15.36% in 2002. The average percentage of albumins and globulins of the total protein reached 36.97% in 2001 and 41.04% in 2002, the average percentage of glutelins of the total proteins was 37.61% in 2001 and 34.10% in 2002, and residual was on average 7.55% in 2001 and 8.70% in 2002, respectively, of the total protein. Electrophoretic analysis of reserve (gluten) proteins (SDS-PAGE ISTA) showed in the oats collection evaluated the percentage of LMW + prolamins in the range 56–77% of the total reserve proteins in 2001, and 52–73% in 2002. The results of A-PAGE electrophoretic analysis of prolamin proteins confirmed the presence of α-prolamins, that ranged in the total content of prolamins from 50 to 88% in 2001, and from 77 to 100% in 2002, while β- + γ-prolamins ranged in 2001 from 11 to 49%, and in 2002 from 0 to 22%. These values do not give serious guarantees for the possible utilisation of oats in the gluten-free diet. The results of the immunological evaluation of the amount of prolamins in oats grains using ELISA showed great differences between different varieties and the experimental years. In 2001, 7 oats samples out of 13 evaluated, and in 2002 10 samples out of 12 evaluated were below the limit for the gluten-free diet (10 mg prolamins (gliadins)/100 g of sample dry matter), but the other varieties exceeded the limit, particularly in 2001, very significantly. The results obtained in the evaluated collection of species and varieties of oats revealed a great variability in the structure of the protein complex and in the immunological testing. In addition a significant effect of the year on the results of all analyses was evident. Based on our results, the use of oats in the diet for coeliac disease can be very risky for these reasons.  

scholarly journals Studying The Chemical Composition Of Apple Fruits Of Different Ecological And Geographical Groups

2020 ◽  
Vol 02 (11) ◽  
pp. 94-100
Author(s):  
Sh.M. Akhmedov ◽  
Keyword(s):  
Ascorbic Acid ◽  
Chemical Composition ◽  
Dry Matter ◽  
Apple Tree ◽  
Titratable Acidity ◽  
Apple Trees ◽  
Breeding Programs ◽  
Apple Varieties ◽  
Apple Fruits

The article presents the results of studying the chemical composition of fruits of different varieties of apple trees. The study of the chemical composition of apple fruits showed that the place of breeding and growth of the variety directly depends on the increase in the chemical composition of the fruit. The following apple varieties were identified: by dry matter - Afghan 255603, Roksa Red Delicious; by the amount of sugars - Georges kav, Jonared; by the amount of sucrose - Carnel Red, Georges Kav, Zarya Podolia, Mariorik Pia; for titratable acidity - Afghan 255603; for ascorbic acid - Grafenstein red, Calville Lezanne, Renet June, Apple-tree Gissar. These varieties can be used in breeding programs and introduced into production.

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