Differences among bread wheat genotypes for tissue nitrogen content and their relationship to grain yield and protein content

1996 ◽  
Vol 47 (1) ◽  
pp. 33 ◽  
Author(s):  
MA Rostami ◽  
L O'Brien
Keyword(s):  
Grain Yield ◽  
Nitrogen Content ◽  
Protein Content ◽  
Grain Protein Content ◽  
Grain Protein ◽  
Genotypic Differences ◽  
Near Infrared Reflectance ◽  
Nitrogen Rate ◽  
Ground Sample ◽  
Tissue Nitrogen

This study was conducted to examine genotypic differences in tissue nitrogen content of wheat and establish the extent to which they were related to variation in grain yield and protein content. Thirty-six genotypes consisting of cultivars and advanced breeding lines were grown at four rates of applied nitrogen: 0, 50, 100 and 200 kg N/ha, for 3 years. Above-ground plant matter was harvested at approximately Zadoks growth stage 31 (commencement of stem elongation), microwaved for 4 min, then oven-dried at 60�C. Tissue nitrogen content of the hammermill-ground sample and grain protein content were determined by Near Infrared Reflectance Spectrometry. Genotypic differences in tissue nitrogen content, grain yield and protein content were established in each year. Maxima for tissue nitrogen and grain protein were obtained at the highest nitrogen rate, whereas the minima were at the zero rate. Yield responses to nitrogen application varied between years. Tissue nitrogen content was positively correlated with grain protein content in 8 of 12 within individual nitrogen rate comparisons. Over all nitrogen rates, the two variables were significantly positively correlated (ranging from r = 0.453 to r = 0.771). Tissue nitrogen content and protein content generally exhibited high heritability estimates within and over years, whereas grain yield had a high within year but a low over years heritability value. The use of tissue nitrogen content provides a possible basis for a screening test to select for yield and protein content in wheat breeding programs.

scholarly journals Pyrenophora teres and Rhynchosporium secalis infections in malt barley as influenced by genotype, spatial and temporal effects and nitrogen fertilization

2019 ◽  
Author(s):  
Petros Vahamidis ◽  
Angeliki Stefopoulou ◽  
Christina S. Lagogianni ◽  
Garyfalia Economou ◽  
Nicholas Dercas ◽  
...  
Keyword(s):  
Grain Size ◽  
Grain Yield ◽  
Protein Content ◽  
Disease Severity ◽  
Crop Residues ◽  
Grain Protein Content ◽  
Grain Protein ◽  
Nitrogen Rate ◽  
Leaf Scald ◽  
Malt Barley

SummaryNet form net blotch (NFNB) and barley leaf scald are among the most important barley diseases worldwide and particularly in Greece. Their occurrence in malt barley can exert a significant negative effect on malt barley grain yield and quality. An experimental trial across two growing seasons was implemented in Greece in order i) to assess the epidemiology of NFNB and leaf scald in a barley disease free area when the initial inoculation of field occurs through infected seeds, and ii) to further explore the relationship among nitrogen rate, grain yield, quality variables (i.e. grain protein content and grain size) and disease severity and epidemiology. It was demonstrated that both NFNB and leaf scald can be carried over from one season to the next on infected seed under Mediterranean conditions. However, disease severity was more pronounced after barley tillering phase when soil had been successfully inoculated first. When nitrogen rate and genotype were the main sources of variation the epidemiology assessment was implemented with hotspot and Anselin Local Moran’s I analysis. It was found that the location of hotspots was modified during growing season. Soil and plant variables were assessed for the explanation of this variability. According to commonality analysis the effect of distance from the locations with the highest disease infections was a better predictor of disease severity (for both diseases) compared to nitrogen rate during pre-anthesis period. However, disease severity after anthesis was best explained by nitrogen rate only for the most susceptible cultivars to NFNB. The effect of disease infections on yield, grain size and grain protein content varied in relation to genotype, pathogen and stage of crop development. The importance of crop residues on the evolution of both diseases was also highlighted.

Variability for grain protein content among germplasm accessions and advanced breeding lines in dolichos bean (Lablab purpureus L. Sweet var. Lignosus Prain)

2018 ◽  
Vol 17 (03) ◽  
pp. 289-292
Author(s):  
Pranesh ◽  
S. Ramesh
Keyword(s):  
Grain Yield ◽  
Protein Content ◽  
Protein Energy Malnutrition ◽  
Target Population ◽  
Grain Protein Content ◽  
Grain Protein ◽  
Lablab Purpureus ◽  
Breeding Lines ◽  
Protein Rich ◽  
Germplasm Accessions

AbstractProtein energy malnutrition (PEM) is prevalent in south-east Asian countries including India. Breeding and introduction of grain protein-rich varieties of legumes such as dolichos bean is considered as cost-effective approach to combat PEM. Exploitation of genetic variability within germplasm accessions (GAs) and/or breeding populations is the short-term strategy for identification and delivery of protein-rich dolichos bean cultivars to cater to the immediate needs of the farmers and target population. A set of 118 dolichos bean genotypes consisting of 96 GAs and 20 advanced breeding lines (ABLs) and two released varieties (RVs) was field evaluated in augmented deign for dry grain yield per plant and their grain protein contents were estimated. The grain protein content among the genotypes ranged from 18.82 to 24.5% with a mean of 21.73%. The magnitude of estimates of absolute range, standardized range, and phenotypic coefficient of variation (PCV) for grain protein content was higher among GAs than those among ABLs + RVs. However, average grain protein contents of GAs were comparable to those of ABLs + RVs. Nearly 50% of the genotypes (mostly GAs) had significantly higher grain protein content than those of RVs, HA 3 and HA 4. The grain protein contents of the genotypes were poorly correlated with grain yield per plant. These results are discussed in relation to strategies to breed grain protein-rich dolichos bean cultivars.

scholarly journals Genome-Wide Identification of QTLs for Grain Protein Content Based on Genotyping-by-Resequencing and Verification of qGPC1-1 in Rice

2020 ◽  
Vol 21 (2) ◽  
pp. 408 ◽  
Author(s):  
Yi-Bo Wu ◽  
Guan Li ◽  
Yu-Jun Zhu ◽  
Yi-Chen Cheng ◽  
Jin-Yu Yang ◽  
...  
Keyword(s):  
Protein Content ◽  
Near Infrared ◽  
Correlation Coefficients ◽  
Grain Protein Content ◽  
Chromosome 1 ◽  
Grain Protein ◽  
Rice Grain ◽  
Near Infrared Reflectance ◽  
Ril Population ◽  
Density Map

To clarify the genetic mechanism underlying grain protein content (GPC) and to improve rice grain qualities, the mapping and cloning of quantitative trait loci (QTLs) controlling the natural variation of GPC are very important. Based on genotyping-by-resequencing, a total of 14 QTLs were detected with the Huanghuazhan/Jizi1560 (HHZ/JZ1560) recombinant inbred line (RIL) population in 2016 and 2017. Seven of the fourteen QTLs were repeatedly identified across two years. Using three residual heterozygote-derived populations, a stably inherited QTL named as qGPC1-1 was validated and delimited to a ~862 kb marker interval JD1006–JD1075 on the short arm of chromosome 1. Comparing the GPC values of the RIL population determined by near infrared reflectance spectroscopy (NIRS) and Kjeldahl nitrogen determination (KND) methods, high correlation coefficients (0.966 and 0.983) were observed in 2016 and 2017. Furthermore, 12 of the 14 QTLs were identically identified with the GPC measured by the two methods. These results indicated that instead of the traditional KND method, the rapid and easy-to-operate NIRS was suitable for analyzing a massive number of samples in mapping and cloning QTLs for GPC. Using the gel-based low-density map consisted of 208 simple sequence repeat (SSR) and insert/deletion (InDel) markers, the same number of QTLs (fourteen) were identified in the same HHZ/JZ1560 RIL population, and three QTLs were repeatedly detected across two years. More stably expressed QTLs were identified based on the genome resequencing, which might be attributed to the high-density map, increasing the detection power of minor QTLs. Our results are helpful in dissecting the genetic basis of GPC and improving rice grain qualities through molecular assisted selection.

scholarly journals Nitrogen Content of Wheat Plants at Anthesis Affects Grain Protein Content and its Increase in Response to Nitrogen Topdressing at Anthesis

2015 ◽  
Vol 84 (2) ◽  
pp. 140-149 ◽  
Author(s):  
Yumi Shimazaki ◽  
Maiko Akasaka ◽  
Yoshiaki Watanabe ◽  
Yasuo Ohshita ◽  
Hiromi Matsuyama ◽  
...  
Keyword(s):  
Nitrogen Content ◽  
Protein Content ◽  
Grain Protein Content ◽  
Grain Protein

Effect of late application of nitrogen on the yield and protein content of wheat

1982 ◽  
Vol 22 (115) ◽  
pp. 54 ◽  
Author(s):  
WM Strong
Keyword(s):  
Grain Yield ◽  
Protein Content ◽  
Foliar Application ◽  
Grain Protein Content ◽  
Growth And Yield ◽  
Grain Protein ◽  
Price Ratio ◽  
N Application ◽  
Boot Stage ◽  
Supplementary Irrigation

On the Darling Downs the growth and yield of a semi-dwarf wheat (cv. Oxley) under supplementary irrigation was increased by the application of up to 400 kg/ha of nitrogen (N) at planting. Nitrogen at 50 or 100 kg/ha applied at the boot stage to supplement 100 kg/ha applied at planting increased grain yield by 459 and 478 kg/ha, respectively. However, yields were still below those where all the N was applied at planting. In contrast, supplementary N (0, 25, 50 or 100 kg/ha) at flowering or after flowering generally did not increase grain yield. One exception to this was where only 50 kg/ha was applied at planting; an additional 100 kg/ha at flowering increased grain yield by 602 kg/ha. Applied at planting, more than 200 kg/ha of N was needed to produce premium grade wheat (i.e. protein content above 11.4%). To achieve this protein content where 100 kg/ha had been applied at planting an additional 100 kg/ha was needed at the boot stage or 50 kg/ha at flowering. Applied after flowering, up to 100 kg/ha of additional N produced wheat of a protein content too low to attract a premium payment. A similar quantity of N was assimilated whether the entire N application was applied at planting or where the application was split between planting and boot or flowering. Less N was assimilated when the application was split between planting and after flowering. More N was assimilated from soil than from foliar applications at the boot stage. Soil and foliar applications were equally effective at flowering in increasing the amount of N assimilated as well as the grain protein content. However, after flowering foliar application was the more effective method. The application of N at flowering to increase the protein content of this semi-dwarf cultivar is not an attractive commercial practice. The price ratio of premium to Australian Standard White wheat in recent years (<1.071 ) is less than that needed (1.0954-1.3013) to justify splitting the N application to lift grain protein content above 11.4% at the expense of yield.

scholarly journals Response of wheat yield and its components to zinc and iron application under different levels of nitrogen

2020 ◽  
Vol 1 (1) ◽  
Author(s):  
Gheith El-Sayed ◽  
◽  
Ola El-Badry ◽  
Keyword(s):  
Grain Yield ◽  
Protein Content ◽  
Plant Height ◽  
Block Design ◽  
Wheat Yield ◽  
Grain Protein Content ◽  
Yield Component ◽  
Grain Protein ◽  
Research Station ◽  
Randomized Complete Block Design

To evaluate the effect of nitrogen, zinc and iron as soil application on yield and yield component of wheat, the present study was conducted at Agricultural and Experimental Research Station at Giza, Faculty of Agriculture Cairo University, Egypt during 2015/2016 and 2016/2017 seasons. The experimental design was split-plot in randomized complete block design with three replications. Results showed that positive significant effect on plant height, number of spike/m2, spike length; number of grain per spike, grain yield per unit area in both seasons and grain protein content in one season were achieved by application of N and the micronutrients. Whoever, the highest significant in the above mentioned characters was obtained either by application the highest N levels (100kg N /fed.) or in addition to mixture of Zn and Fe. The interaction between the studied factors had significant effect on plant height and grain yield in both seasons as well as on grain protein content in the second season, where the highest values of these parameters were recorded by application of 100kg N/fed., Zn and Fe in mixture.

Grain protein and grain yield of durum wheats from south-eastern Anatolia, Turkey

2000 ◽  
Vol 51 (6) ◽  
pp. 665 ◽  
Author(s):  
M Koç ◽  
C. Barutçular ◽  
N. Zencirci
Keyword(s):  
Grain Yield ◽  
Protein Content ◽  
Environmental Effect ◽  
Triticum Turgidum ◽  
Grain Protein Content ◽  
Protein Yield ◽  
Grain Protein ◽  
Eastern Anatolia ◽  
Breeding Programs ◽  
Crop Varieties

High grain protein in durum wheat [Triticum turgidum ssp. turgidum L. conv. Durum (Desf.)] is one of the main goals of breeding programs. Landraces may be very useful germplasm for achieving this goal. To examine their potential as a source of high grain protein content, 11 genotypes, including 7 landraces, were evaluated in 8 environments. Environment, genotype, and the interaction of the two (G E) significantly influenced the variation in grain yield, grain protein content, and grain protein yield. The environmental effect was the strongest, mostly due to differences in water supply. Grain yields of the modern genotypes were higher than those of landraces. Yields of the modern genotypes tended to respond more strongly to the higher yielding environments, but they varied more than the yields of landraces. With the exception of VK.85.18, the grain protein content of the high-yielding genotypes was almost as high as that of the best landraces. Moreover, grain protein content of these bred genotypes tended to respond more strongly to the higher protein environments. Differences in grain protein yield were closely related to the differences in grain yield. The results indicate that it is possible to improve grain protein content without grain yield being adversely affected. The results also indicate that potential gene sources should be compared over a number of environments before they can be used as breeding material or as crop varieties producing high grain protein yields.

scholarly journals Nitrogen Metabolism at Tillering Stage Differently Affects the Grain Yield and Grain Protein Content in Two Durum Wheat Cultivars

Diversity ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 186
Author(s):  
Fortunato ◽  
Nigro ◽  
Paradiso ◽  
Cucci ◽  
Lacolla ◽  
...  
Keyword(s):  
Amino Acids ◽  
Grain Yield ◽  
Durum Wheat ◽  
Protein Content ◽  
Grain Protein Content ◽  
Cultivar Differences ◽  
Grain Protein ◽  
Wheat Cultivars ◽  
Nitrogen Use ◽  
Nitrogen Abundance

Soil nitrogen abundance, as well as nitrogen use efficiency (NUE), significantly affect the crop yield and grain protein content (GPC). Depending on the genotype, a negative correlation between the yield and GPC can occur. The aim of the study was to assess the agronomic performance, and to explore physiological pathways for the efficient use of N fertilizer for two durum wheat cultivars, “Aureo” and “Vespucci”. After fertilization, the nitrogen content and values of some of the agronomic parameters and yield-related traits increased in both cultivars; nevertheless, a simultaneous rise in both the yield and GPC occurred only in Aureo. The biochemical parameters, analyzed at tillering, confirm the genotypic specificity of nitrogen use. In Vespucci’s roots, the nitrogen supply did not affect the nitrate reductase (NR), but greatly increased the amino acids and proteins, suggesting that ammonium is preferentially assimilated. In Aureo, nitrate is in part assimilated by the roots, as suggested by the ammonium increase and NR enhancement. In the leaves of both cultivars, organic nitrogen significantly increased after fertilization; however, the rise in amino acids, as well as in NR activity, was higher in Aureo than in Vespucci. These results indicate that the different nitrogen use, and in particular the diverse NR behavior, at tillering, are in part responsible of the cultivar differences in grain yield and GPC.

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