Paucity of somaclonal variation from immature embryo culture of barley

1989 ◽  
Vol 40 (6) ◽  
pp. 1155 ◽  
Author(s):  
DJ Luckett ◽  
D Rose ◽  
E Knights
Keyword(s):  
Tissue Culture ◽  
Somaclonal Variation ◽  
Embryo Culture ◽  
Field Trial ◽  
Immature Embryo ◽  
Immature Embryos ◽  
Golden Promise ◽  
Immature Embryo Culture ◽  
Tissue Origin ◽  
Regenerant Plants

Intact immature embryos of barley (cv. Golden Promise) and component tissues (the scutellum and embryonic axis) were cultured to produce callus. Regenerant plants were obtained from this callus and SC2 families raised. These families were examined in a field trial to search for somaclonal variation. No obvious variants were found confirming our previous unpublished results. The lack of somaclonal variation generated by barley tissue culture (which is in contrast to other species) was not a result of the tissue origin of the regeneration event.

Somaclonal variation from cultured immature embryos of sister lines of rye differing in heterochromatic content

Genome ◽  
1990 ◽  
Vol 33 (2) ◽  
pp. 177-183 ◽  
Author(s):  
P. J. Bebeli ◽  
A. Karp ◽  
P. J. Kalts1kes
Keyword(s):  
Somaclonal Variation ◽  
Storage Proteins ◽  
Immature Embryo ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Immature Embryos ◽  
Chromosome Variation ◽  
Regenerated Plants ◽  
Immature Embryo Culture ◽  
Telomeric Heterochromatin

Somaclonal variation occurred among rye plants regenerated from cultured immature embryos of five sister lines that differed in their content of telomeric heterochromatin. Variation was observed in morphology, chromosome number, and secalin seed storage proteins. Morphological variation was present in 9.7% of the regenerants and included albinism and variegation, which appeared in different frequencies among the lines. Chromosome variation occurred in 15.8% of the regenerants and included translocations, tetraploidy, and trisomy in addition to meiotic disturbances such as centromere misbehaviour and asyndesis. Some of the regenerated plants were mosaic for the structural and numerical chromosome aberrations. The nature of the chromosome variation also differed among the lines. A single variant in the 40K γ-secalins was detected. The occurrence of variation is discussed in relation to differences in morphogenetic response of the rye lines and to the genotypic component of instability in culture.Key words: somaclonal variation, immature embryo culture, rye heterochromatin, chromosome variation, secalins.

Relative stability among barley plants regenerated from cultured immature embryos

Genome ◽  
1987 ◽  
Vol 29 (3) ◽  
pp. 405-412 ◽  
Author(s):  
A. Karp ◽  
S. H. Steele ◽  
S. Parmar ◽  
M. G. K. Jones ◽  
P. R. Shewry ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Somaclonal Variation ◽  
Length Polymorphism ◽  
Immature Embryos ◽  
Glutamate Oxaloacetate Transaminase ◽  
Spacer Length ◽  
Factors Affecting ◽  
Regenerated Plants ◽  
Key Words Barley ◽  
Sds Page

Relative genetic stability was observed among barley plants regenerated from cultured immature embryos. Regenerated plants were studied cytologically and their seed progenies assayed for (i) the isoenzymes esterase and glutamate-oxaloacetate transaminase, (ii) ribosomal DNA spacer length polymorphism, and (iii) hordein patterns on SDS–PAGE. Of 42 regenerated plants, 1 regenerant had abnormal meiosis and the same plant produced one seed with a variant hordein pattern. These findings are discussed in relation to the factors affecting somaclonal variation in cereals and to methods of assaying the variation. Key words: barley, isozymes, somaclonal variation, tissue culture.

Optimisation of Tissue Culture Conditions for Transformation Studies Using Immature Embryos of Australian Barley Cultivars

1995 ◽  
Vol 43 (5) ◽  
pp. 499 ◽  
Author(s):  
AM Walmsley ◽  
RJ Henry ◽  
RG Birch
Keyword(s):  
Tissue Culture ◽  
Plant Regeneration ◽  
Culture Medium ◽  
Immature Embryo ◽  
Culture Conditions ◽  
Medium Composition ◽  
Immature Embryos ◽  
Systematic Testing ◽  
Barley Cultivars ◽  
Key Variables

Eight Australian barley cultivars were tested for efficiency of embryonic callus initiation and plant regeneration, from immature embryo explants in tissue culture. Optimisation of tissue culture conditions was performed for cultivars Bandulla, Clipper, Schooner and Tallon in an attempt to increase regeneration frequencies to levels suitable for genetic engineering of barley. Variables tested were 2,4-D concentration, salt composition, carbon source and immature embryo explant. Optimal culture medium composition varied between cultivars. Shoot regeneration rates from culture of isolated scutellar tissues were low for all four cultivars. Halved, immature embryos produced most shoots for cultivars Clipper, Schooner and Tallon, whereas Bandulla performed best with entire immature embryo explants. Clipper (a malting barley) and Bandulla (a feed barley) are suggested as model Australian cultivars for transformation studies. Immature embryos of Bandulla produced an average of 5.3 shoots and Clipper 10.1 shoots per embryo under optimal conditions. Our results show that rates of somatic embryo and plant regeneration sufficient for use in transformation studies can be achieved for diverse Australian Barley cultivars, through systematic testing of a range of key variables including explant type and medium composition.

scholarly journals Variations in the Gliadin Composition in Immature Embryo Culture-derived Somaclonal Lines of Durum Wheat.

1999 ◽  
Vol 16 (2) ◽  
pp. 167-170
Author(s):  
Naoko YOKOUCHI ◽  
Nobuaki ASAKURA ◽  
Sergei TSVETANOV ◽  
Naoki MORI ◽  
Atanas ATANASSOV ◽  
...  
Keyword(s):  
Durum Wheat ◽  
Embryo Culture ◽  
Immature Embryo ◽  
Immature Embryo Culture

scholarly journals Effect of Genotyps and Polyethylene Glycol on Immature Embryo Culture in Wheat

2018 ◽  
Vol 9 (8) ◽  
pp. 175-180
Author(s):  
Rehab Habiba ◽  
A. El-Adl ◽  
S. Abd El-Mageed ◽  
Kh. Gomaa
Keyword(s):  
Polyethylene Glycol ◽  
Embryo Culture ◽  
Immature Embryo ◽  
Immature Embryo Culture
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