New records on the occurrence of beet western yellows virus in Australia, New Zealand and Mexico

1989 ◽  
Vol 40 (2) ◽  
pp. 353 ◽  
Author(s):  
GR Johnstone ◽  
JE Duffus ◽  
PL Guy
Keyword(s):  
New Zealand ◽  
Freeze Drying ◽  
New Records ◽  
Leaf Roll ◽  
Leaf Tissue ◽  
Potato Leaf ◽  
Beet Western Yellows Virus ◽  
Elisa System ◽  
The North ◽  
Das Elisa

An isolate of beet western yellows virus (BWYV) from lettuce in Tasmania was propagated in shepherd's purse, purified, and used to produce an antiserum in a rabbit. The lettuce isolate and the antiserum to it reacted similarly to the Californian type isolate from radish and its antiserum in double antibody sandwich enzyme-linked immunosorbent assays (DAS-ELISA). The Tasmanian DAS-ELISA system was used to confirm the presence of BWYV in a range of plant species from the southern mainland states of Australia, from the North Island of New Zealand and from central Mexico. Leaf tissue containing BWYV remained serologically reactive for long periods after the tissue was desiccated either by freeze-drying, air-drying or drying over silica gel. Bean leaf roll, potato leaf roll and soybean dwarf viruses were clearly distinct from BWYV and from each other in DAS-ELISA.

scholarly journals Presence and distribution of economically important potato viruses in Montenegro

2011 ◽  
Vol 26 (2) ◽  
pp. 117-127
Author(s):  
Jelena Zindovic
Keyword(s):  
Potato Virus ◽  
Leaf Roll ◽  
Potato Virus X ◽  
Enzyme Linked Immunosorbent Assay ◽  
Potato Leaf ◽  
Potato Virus S ◽  
Potato Varieties ◽  
Potato Viruses ◽  
Das Elisa

The research was carried out, in the period 2002-2004 in order to determine the presence and distribution of potato viruses at 12 different locations and on 9 different potato varieties grown in Montenegro. The research included collecting of samples in seed potato crops and testing of six economically important potato viruses: Potato leaf roll virus (PLRV), Potato virus Y (PVY), Potato virus X (PVX), Potato virus S (PVS), Potato virus A (PVA) i Potato virus M (PVM). Using the direct enzyme-linked immunosorbent assay (DAS-ELISA) and commercial antisera specific for six potato viruses, it was found that PVY was the most frequent virus during the three-year research period. The second frequent virus was PVS, followed by PVA, PLRV, PVM and PVX. Single and mixed infections were detected, and the most prevalent were the single infections of PVY. Also, in the period 2002-2004, PVY had the highest distribution and the number of present viruses was different at different localities and on different potato varieties. Further investigations were related to detailed characterization of the most prevalent virus (PVY), which is at the same time economically the most important one. Serological characterization of PVY was performed utilizing DAS-ELISA kit with commercial monoclonal antibodies specific for detection of the three strain groups of PVY, and the two strain groups - necrotic (PVYN/PVYNTN) and common (PVYO), were identified. Necrotic strains were prevalent in 2002 and 2004, while in 2003 PVYO was the most frequent strain in virus population. The presence of stipple streak strain (PVYC) was not detected in any of the tested samples.

scholarly journals Virus Incidence Associated with Native Potato Yield in Microcenters of Potato Genetic Diversity of Bolivian

2021 ◽  
Author(s):  
Mario Coca Morante ◽  
Evelyn Coca Salazar ◽  
Javier Burgos Villegas ◽  
Nelson Tapia Ponce
Keyword(s):  
Genetic Diversity ◽  
Viral Infection ◽  
Potato Virus ◽  
Leaf Roll ◽  
Potato Virus X ◽  
Potato Yield ◽  
Potato Leaf ◽  
La Paz ◽  
Andean Potato ◽  
Das Elisa

AbstractIn the Bolivian Andean region, a diversity native potatoes species (Solanum spp) are cultivated. Areas where many types of native potato are grown are known as microcentres of potato genetic diversity. However, yields are low in these places, apparently related to viral infections. The incidence of the potato-affecting viruses Potato Virus X (PVX), Potato Virus Y (PVY), Potato Leaf Roll Virus (PLRV), Andean Potato Latent Virus (APLV) and Andean Potato Mottle Virus (APMoV) was examined by DAS-ELISA in microcentres of two high Andean regions of Bolivia, i.e. an Aymara culture region in the north of La Paz, and a Quechua culture region in the Dept. of Cochabamba. The relationship between viral incidence and potato yield was explored as well. The presence of all five viruses was detected in both regions, except APMoV in the Aymara region. The mean overall incidence of viral infection was greater in the Aymara than in the Quechua region. The most commonly detected viruses were PVX, PVY and APLV. Mix-infections of PVY + PVX and PVY + PLRV were most common in the Aymara region, and PVX + APLV and PVX + APMoV in the Quechua region. Potato yield was less in Aymara than in Quechua region and it was correlated to higher incidence of PVY and PVX in Aymara region. Low yields and high incidence of viral infection seems to be related to the ancestral management by planting native seed potatoes of poor quality. In conclusion, four of the five viruses sought were detected in the Aymara region, and all five in the Quechua region, although the overall incidence of viral infection was greater in the Aymara region, where the yields recorded were also lower.Resumen.En la región andina boliviana se cultivan una diversidad de especies nativas de papa (Solanum spp). Las áreas donde se cultivan muchos tipos de papa nativa se conocen como microcentros de diversidad genética de la papa. Sin embargo, los rendimientos son bajos en estos lugares, aparentemente relacionados con infecciones virales. Se examinó la incidencia de los virus que afectan a la papa, el Virus X de la Papa (PVX), Virus Y de la Papa (PVY), Virus del Rollo de hoja de Papa (PLRV), Virus Latente de la Papa Andina (APLV) y Virus moteado de la Papa Andina(APMoV) por DAS-ELISA en microcentros de dos regiones altoandinas de Bolivia, por ejemplo, una región de cultivo Aymara en el norte de La Paz y una región de cultivo Quechua en el Departamento de Cochabamba. También se exploró la relación entre la incidencia viral y el rendimiento de la papa. Se detectó la presencia de los cinco virus en ambas regiones, excepto APMoV en la región Aymara. La incidencia global media de infección viral fue mayor en la región Aymara que en la región Quechua. Los virus más comúnmente detectados fueron PVX, PVY y APLV. Las infecciones por mezcla de PVY + PVX y PVY + PLRV fueron más comunes en la región Aymara, y PVX + APLV y PVX + APMoV en la región Quechua. El rendimiento de papa fue menor enAaymara que en la región Quechua y se correlacionó con una mayor incidencia de PVY y PVX en la región Aymara. Estamos evidenciando que los bajos rendimientos y la alta incidencia de infección viral parecen estar relacionados con el manejo ancestral mediante la siembra de papas de semilla nativa de mala calidad. En conclusión, cuatro de los cinco virus buscados se detectaron en la región Aymara, y los cinco en la región Quechua, aunque la incidencia general de infección viral fue mayor en la región Aymara, donde los rendimientos registrados también fueron menores.

Some luteovirus diseases in Tasmania caused by beet western yellows and subterranean clover red leaf viruses

1984 ◽  
Vol 35 (6) ◽  
pp. 821 ◽  
Author(s):  
GR Johnstone ◽  
JE Duffus
Keyword(s):  
North America ◽  
Sugar Beet ◽  
Host Range ◽  
Leaf Roll ◽  
Subterranean Clover ◽  
Potato Leaf ◽  
Beet Western Yellows Virus ◽  
Pasture Legumes ◽  
Natural Hosts ◽  
Leaf Virus

A range of crop plants, pasture legumes and weeds, mostly with yellows symptoms similar to those caused by luteoviruses, were collected from the field around Tasmania and checked for infection with beet western yellows virus (BWYV) and subterranean clover red leaf virus (SCRLV) using aphids and indicator plants. BWYV was recovered from 216 of 897 plants tested, representing 30 different species and including 16 not previously recorded as natural hosts. SCRLV was recovered from 163 of 637 plants, representing twelve species including four not previously recorded as natural hosts. BWYV was isolated most often from composites and crucifers, while SCRLV was recovered most frequently from legumes. Eight plants were found infected with both viruses together. In host range studies, Tasmanian isolates of BWYV caused symptoms in lettuce, subterranean clover and sugar beet like those seen on these plants in the field from which the virus was isolated, and were thus similar to isolates of BWYV from North America. The Tasmanian isolates of BWYV were also closely related serologically to Californian isolates of BWYV; these were serologically quite distinct from isolates of SCRLV, and both these groups were serologically distinct from legume yellows virus and from potato leaf roll virus.

scholarly journals A virus survey of Vicia faba crops in Canterbury New Zealand during 201112

2013 ◽  
Vol 66 ◽  
pp. 382-382
Author(s):  
J.D. Fletcher ◽  
H. Ziebell ◽  
R.M. MacDiarmid
Keyword(s):  
New Zealand ◽  
Mosaic Virus ◽  
Vicia Faba ◽  
Cropping Systems ◽  
Leaf Roll ◽  
Alfalfa Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Field Bean ◽  
Related Field ◽  
Beet Western Yellows Virus

Broad bean (Vicia faba L) is an established vegetable crop grown in Canterbury with the area now growing related field bean for both human and animal consumption increasing and forming a useful addition to mixed cropping systems A V faba virus survey completed in 1991 detected Soybean dwarf virus (SDV) and Beet western yellows virus (BWYV) Turnip yellows virus (TuYV) which cause bean leaf roll; Alfalfa mosaic virus (AMV); Cucumber mosaic virus (CMV); Pea seedborne mosaic (PSbMV); and Bean yellow mosaic virus (BYMV) In 2011 16 faba bean crops throughout mid and South Canterbury were surveyed for viruses known and not known to be present in New Zealand Virus incidences were low with only a few crops damaged largely by bean leaf roll When compared with previous surveys only TuYV appears to have become more widespread but with a similar incidence (07) SDV was less widespread but had a higher incidence (025) The incidences of other viruses were similar to the previous survey AMV (09) PSbMV (035) BYMV (05) although CMV was not detected Red clover vein mosaic virus (RCVMV) was detected for the first time in for New Zealand and was found to be reasonably widespread and at high incidences within some crops

The probable long association of beet western yellows virus with the potato leaf roll syndrome in Tasmania

1982 ◽  
Vol 22 (117) ◽  
pp. 353 ◽  
Author(s):  
JE Duffus ◽  
GR Johnstone
Keyword(s):  
World Wide ◽  
Leaf Roll ◽  
Potato Cultivars ◽  
Wide Spread ◽  
Potato Leaf ◽  
Serological Tests ◽  
Plant Quarantine ◽  
Certification Scheme ◽  
Beet Western Yellows Virus ◽  
Potato Culture

Beet western yellows virus (BWYV) was isolated from potato cultivars showing leaf roll in Tasmania. Twenty-four of 25 plants representing 11 cultivars selected from the field as showing typical leaf roll symptoms contained virus that infected Capsella bursa-pastoris. Serological tests with two isolates showed them to be closely related to several Californian BWYV isolates. Because of strict plant quarantine and certification schemes operating in Tasmania since the early 1930s, these isolates of BWYV are probably representative of leaf roll isolates that were common before world-wide certification schemes. The occurrence of BWYV in potatoes during these early periods strongly indicates a world-wide significance of BWYV in potato culture. The wide spread occurrence of BWYV associated with leaf roll in Tasmanian potatoes could have very important consequences for the potato certification scheme. These are discussed together with the use of sensitive serological tests to detect luteoviruses in potato and facilitate their control.

Utilization of Shoot Cuttings for Elimination of PLRV and PVY by Thermotherapy and Chemotherapy from Potato (Solanum tuberosum L.)

2007 ◽  
Vol 7 ◽  
pp. 1
Author(s):  
S. P. Dhital ◽  
B. M. Sakha ◽  
H. T. Lim
Keyword(s):  
Plant Height ◽  
Solanum Tuberosum L ◽  
Combined Treatment ◽  
Leaf Roll ◽  
Enzyme Linked Immunosorbent Assay ◽  
Potato Leaf ◽  
Virus Elimination ◽  
Elisa Testing ◽  
Das Elisa

Heat and ribavirin treatments were applied for the elimination of potato leaf roll virus (PLRV) and potato virus Y (PVY) from the potato genotype F 9-99. The explants, rooted young plantlets, cultured on MS medium with and without ribavirin (20 mg l<sup>-1</sup>) were subjected to thermotherapy (35ºC/31ºC, 4 h alternating periods) and room temperature (25ºC) for 30 days. Double antibody sandwich - enzyme linked immunosorbent assay (DAS-ELISA) testing following the therapies revealed that ribavirin alone was enable to eliminate 10% each of PLRV and PVY, whereas along with thermotherapy its efficacy increased to 25% PLRV and 20% PVY elimination. In another experiment, three potato genotypes F 9-99 infected with PLRV and PVY, Gui Valley with PLRV infection and Rose Valley with PVY infection were evaluated. Ribavirin (20 mg l<sup>-1</sup>) and ASA (acetylsalicylic acid) (10<sup>-5</sup> M) were supplemented in liquid culture medium alone or in combination with thermotherapy for three successive cycles of 30-36 days interval. Heat and/or ribavirin suppressed survival and plant height whereas ASA promoted the survival as well as plant height even under heat treatment. After each cycle, the effect of treatment on virus elimination was evaluated by DAS-ELISA. The combined application of ribavirin and ASA with thermotherapy after three cycles of treatment showed up to 47.4% PLRV and 57.9% PVY elimination in F 9-99. In the case of single viral infection of PLRV or PVY the same combined treatment showed up to 58.8% PLRV and 61.1% PVY free plantlets. Virus elimination was also confirmed by transplanting in vitro grown plantlets in a net house and then retesting after 45 days of in vivo growth. This novel technique would be highly efficient for virus elimination within a short duration in diverse genotypes of potato. <i>Nepal Journal of Science and Technology </i> 7 2006

scholarly journals Ocorrência de vírus em batata em sete estados do Brasil

2009 ◽  
Vol 27 (4) ◽  
pp. 490-497 ◽  
Author(s):  
Antônio Carlos de Ávila ◽  
Paulo Eduardo de Melo ◽  
Lindolfo R Leite ◽  
Alice K Inoue-Nagata
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Leaf Roll ◽  
Potato Virus X ◽  
Potato Leaf Roll Virus ◽  
Rt Pcr ◽  
Potato Leaf ◽  
Potato Virus S ◽  
Das Elisa

As viroses causam rápida degenerescência dos tubérculos-sementes de batata. Em condições tropicais, em que a presença de afídeos vetores é constante e a estrutura das populações de vírus é dinâmica, a pressão das doenças é enorme. Conhecer essa dinâmica é uma ferramenta importante para a sustentabilidade da produção de batata. Realizou-se um levantamento abrangente da ocorrência de viroses em batata no Brasil, além de estudar-se a distribuição das estirpes de Potato virus Y (PVY) associadas ao mosaico da batata. Em 2005 e 2006 foram visitadas lavouras em sete estados brasileiros, coletando-se folíolos com sintomas de viroses (1.256 amostras) e amostras aleatórias (360 amostras). Foi feita também uma estimativa visual da incidência de mosaico e enrolamento-das-folhas em vários dos campos visitados. Das 1.256 amostras suspeitas, 840 apresentaram reação positiva em teste sorológico para PVY (66,9%), 128 para Potato leaf roll virus (PLRV) (10,2%), 79 para Potato virus S (PVS) (6,3%) e nenhuma para Potato virus X (PVX). Os resultados dos testes de detecção por DAS-ELISA, biológico e RT-PCR mostraram a presença quase absoluta do subgrupo necrótico de PVY, em sua maioria PVY NTN. A análise de uma sub-amostragem em todos os municípios visitados confirmou que essa variante está hoje presente nos sete estados visitados. Amostras de PVY NTN foram obtidas das cultivares Asterix, Atlantic, Agata, Achat, Baronesa, Baraka, Bintje, Caesar, Cupido, Marijke, Monalisa, Panda e Vivaldi, que apresentaram diferentes níveis de suscetibilidade. As amostras aleatórias revelaram um quadro muito similar ao encontrado com as amostras sintomáticas. PLRV foi identificado em MG, BA, PR e SC, em várias lavouras de forma muito freqüente. PVS foi identificado nesses mesmos estados e também em SP. PVX foi detectado em apenas uma amostra tomada ao acaso em Serra do Salitre (MG). O contraste entre a avaliação visual dos sintomas e os resultados do teste de detecção por ELISA revelou a possibilidade de infecção latente por PVY em níveis relevantes na cultivar Asterix.

scholarly journals First Report of Five Viruses Infecting Potatoes in Tanzania

Plant Disease ◽  
2013 ◽  
Vol 97 (9) ◽  
pp. 1260-1260 ◽  
Author(s):  
E. Chiunga ◽  
J. P. T. Valkonen
Keyword(s):  
United Kingdom ◽  
Smallholder Farmers ◽  
Solanum Tuberosum L ◽  
Leaf Roll ◽  
Potato Production ◽  
Rt Pcr ◽  
Potato Leaf ◽  
Seed Potatoes ◽  
Potato Crops ◽  
Das Elisa

Potato (Solanum tuberosum L.) is an increasingly important food and cash crop in Tanzania (3). Potato production is concentrated in the southern highlands and mainly carried out by smallholder farmers. A certification system for seed potatoes does not exist in the country. Currently, there is little information about viruses infecting potatoes in Tanzania. In October through December 2011, occurrence of the most common, globally distributed potato viruses, Potato leaf roll virus (PLRV), Potato virus A (PVA), M (PVM), S (PVS), Y (PVY), and X (PVX) (1), was determined in 219 potato plants in 16 fields ranging from 0.2 to 1 ha. Potato crops, 1 to 3 months old, consisted sometimes of mixtures of varieties identified as Arika, Chekundu, Kagiri, Kiazi, Kikondo, Sasamka, or Tigoni by farmers, but could not be independently confirmed. The fields were located in the regions of Mbeya (Kawetele, Kikondo, Umalia, Uyole) and Rungwe (Mwakaleli) ~100 km apart in the southern highlands. Virus-like symptoms observed in most fields included yellowish-green mosaic, leaf rolling, and veinal necrosis. Symptoms in tubers were not studied. Leaves from 10 symptomatic and three symptomless plants were sampled from each field and tested by double antibody sandwich (DAS)-ELISA (1) at ARI-Uyole. Virus-specific antibodies and negative and positive controls were used according to the supplier's instructions (Science and Advice for Scottish Agriculture, Edinburgh, United Kingdom). PVS and PLRV were detected in 55% and 39% of the samples, respectively, and in all fields sampled. PVX and PVM were found in most fields and in 14% and 5% of the samples, respectively. PVA and PVY were only detected in two localities. Co-infection with PVS and PLRV was detected in 14% of the tested plants. Mixed infections involving three or four viruses were detected in 5% of the plants. A total of 20 samples, which were collected from Uyole and Mwakeleli and found to be ELISA-positive for one or several viruses, were pressed on FTA cards (GE Healthcare, Buckinghamshire, United Kingdom), transported to University of Helsinki, and analyzed by reverse-transcription PCR (2) using virus-specific primers designed to amplify the coat protein (CP) encoding region. All ELISA-positive samples tested positive by reverse transcriptase (RT)-PCR. Four and five samples ELISA-negative for PVX or PVA, respectively, were positive when tested by RT-PCR, suggesting that the actual incidence of these viruses may be higher than detected by DAS-ELISA. The PCR products from three to five samples per virus were sequenced without cloning, which reconfirmed detection of PLRV, PVA, PVS, PVX, and PVM (GenBank Accession Nos. KC866618 through KC866622, respectively) and revealed few if any differences among isolates of the viruses. The CP sequences were compared with viruses from other countries and continents (4). CP similarities suggested that viruses might have been introduced to Tanzania through potato trade or through introducing new cultivars without adequate indexing for viruses. These results suggest the need for the development of virus control schemes in potato crops, including the nascent, domestic certified seed potato production in Mbeya. References: (1) G. Loebenstein et al., eds. Virus and Virus-Like Diseases of Potatoes and Production of Seed Potatoes. Kluwer, Dortrecht, Netherlands, 2001. (2) J. Ndunguru et al. Virol. J. 2:45, 2005. (3) J. Rahko. Potato Value Chain in Tanzania. Univ. Helsinki, Finland, 2012. (4) K. Tamura et al. Mol. Biol. Evol. 28:2731, 2011.

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