Identification of the oestrogenic isoflavones in fresh and fermented berseem clover (Trifolium alexandrinum)

1982 ◽  
Vol 33 (6) ◽  
pp. 951 ◽  
Author(s):  
MN Shehata ◽  
A Hassan ◽  
K El-Shazly
Keyword(s):  
Adverse Effects ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Rumen Fluid ◽  
Farm Animals ◽  
Biochanin A ◽  
Trifolium Alexandrinum ◽  
Low Concentrations ◽  
Berseem Clover ◽  
Layer Chromatography

A thin layer chromatography technique was used to identify and semiquantitate the oestrogenic isoflavones in berseem clover (Trifolium alexandrinum). Biochanin A and genistein were the most prominent isoflavones in all the three cuts of fresh berseem clover. Low concentrations of formononetin and daidzein were also detected. The concentrations of the all oestrogenic isoflavones gradually decreased in the successive cuts. Fermentation of all three fresh cuts in silo or in rumen fluid increased the concentrations of these isoflavones. Incubation of the silage materials with rumen fluid had little effect on the concentrations of the isoflavones. The concentrations of the oestrogenic isoflavones in fresh or fermented materials considered were too small to have any adverse effects on growth or reproduction of farm animals.

Identification of phyto-oestrogens in berseem clover (Trifolium alexandrinum)

1976 ◽  
Vol 87 (3) ◽  
pp. 467-469 ◽  
Author(s):  
M. Shemesh ◽  
N. Ayalon ◽  
H. R. Lindner
Keyword(s):  
Gas Chromatography ◽  
Thin Layer ◽  
Dry Matter ◽  
Biochanin A ◽  
Trifolium Alexandrinum ◽  
Berseem Clover

SummaryThe oestrogenic isoflavones genistein, biochanin-A and formononetin, as well as the coumestan derivative coumestrol, were identified by thin-layer, paper and gas chromatography in berseem clover (Trifolium alexandrinum) grown in Israel. Under the conditions studied, the concentration of these oestrogens in the berseem clover (total < 0·01% in the dry matter) was considerably lower than those found in clover species known to be associated with infertility in ruminants.

The oestrogenic isoflavones of subterranean clover

1964 ◽  
Vol 15 (2) ◽  
pp. 223 ◽  
Author(s):  
AB Beck
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Subterranean Clover ◽  
Field Conditions ◽  
Biochanin A ◽  
Isoflavone Content ◽  
Plant Data ◽  
Layer Chromatography

A number of strains of subterranean clover has been examined for isoflavone content in an attempt to explain the differences of oestrogenic potency in sheep and cattle under field conditions. A method of thin-layer chromatography has been developed which allows a rapid semiquantitative estimation of the main isoflavones present in this species. The isoflavones are present in a combined form, probably as glycosides, and the free isoflavones are rapidly released after crushing the leaf. The percentages of formononetin, genistein, and biochanin A in the leaves are very much higher than reported by earlier workers and vary widely between strains. Moreover, the ratios of the isoflavones differ greatly among strains. The relevance of these plant data to the oestrogenic potency in the animal is discussed.

scholarly journals ANALYSIS OF SYNTHETIC ADULTERANTS, MEAN WEIGHT AND LABELING IN TRADITIONAL PHYTOTHERAPIC PRODUCTS

2020 ◽  
Vol 12 (2) ◽  
pp. 24-34
Author(s):  
Bruno Vincenzo Fiod Riccio ◽  
Pamella Cristina Oliveira Françóia ◽  
Paula Olsen Sorgatto ◽  
Airton Vicente Pereira ◽  
Stella Bortoli
Keyword(s):  
Natural Products ◽  
Adverse Effects ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Biologically Active ◽  
Average Weight ◽  
Synthetic Drugs ◽  
Layer Chromatography ◽  
Harmful Effects

Traditional phytotherapic products (TPP) use is nowadays common in society. As they are known to be natural products, there is an assumption that the effects are exclusively beneficial and the potential harmful effects are disregarded. As well as adulterations with synthetic drugs capable of masking adverse effects or acting synergistically with biologically active compounds, potentiating their effects, considered by law as fraud. The aim of the present study was to evaluate common TPP and its compliance with the current law regarding labeling, average weight and research on synthetic adulterants using comparative thin layer chromatography. Twenty PTFs were evaluated, of which all failed the labeling assessment, one contained average weight consistent with legislation and there was no evidence of adulteration by increment of synthetic drugs in any of them. Evaluating the quality of a product ensuring its compliance with the legislation is crucial for the reliability of consumers and the credibility of producers.

Thin-Layer Chromatography

1965 ◽  
Author(s):  
H. R. Bolliger ◽  
M. Brenner ◽  
H. Gänshirt ◽  
Helmut K. Mangold ◽  
H. Seiler ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Layer Chromatography

THE LESS-POLAR METABOLITES PRODUCED BY INCUBATION OF TESTOSTERONE-4-14C WITH RAT AND BOVINE BRAIN

1969 ◽  
Vol 61 (4) ◽  
pp. 641-648 ◽  
Author(s):  
Leon J. Sholiton ◽  
Emile E. Werk
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Paper Chromatography ◽  
Specific Activity ◽  
Bovine Brain ◽  
Polar Fraction ◽  
End Products ◽  
Polar Metabolites ◽  
Layer Chromatography ◽  
Constant Specific Activity

ABSTRACT Rat and bovine brain have been incubated with testosterone-4-14C under standard conditions. With use of paper chromatography, the extracted metabolites were noted to fall into less-polar, iso-polar, and more polar fractions. The components of the less-polar fraction were separated by acetylation and thin-layer chromatography and the major end-products identified by recrystallization to constant specific activity or constant 3H/14C ratios. Androst-4-enedione and 5α-dihydrotestosterone were formed consistently under the conditions utilized. Trace amounts of other less-polar metabolites were noted occasionally.

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