The oestrogenic isoflavones of subterranean clover

1964 ◽  
Vol 15 (2) ◽  
pp. 223 ◽  
Author(s):  
AB Beck
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Subterranean Clover ◽  
Field Conditions ◽  
Biochanin A ◽  
Isoflavone Content ◽  
Plant Data ◽  
Layer Chromatography

A number of strains of subterranean clover has been examined for isoflavone content in an attempt to explain the differences of oestrogenic potency in sheep and cattle under field conditions. A method of thin-layer chromatography has been developed which allows a rapid semiquantitative estimation of the main isoflavones present in this species. The isoflavones are present in a combined form, probably as glycosides, and the free isoflavones are rapidly released after crushing the leaf. The percentages of formononetin, genistein, and biochanin A in the leaves are very much higher than reported by earlier workers and vary widely between strains. Moreover, the ratios of the isoflavones differ greatly among strains. The relevance of these plant data to the oestrogenic potency in the animal is discussed.

Identification of the oestrogenic isoflavones in fresh and fermented berseem clover (Trifolium alexandrinum)

1982 ◽  
Vol 33 (6) ◽  
pp. 951 ◽  
Author(s):  
MN Shehata ◽  
A Hassan ◽  
K El-Shazly
Keyword(s):  
Adverse Effects ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Rumen Fluid ◽  
Farm Animals ◽  
Biochanin A ◽  
Trifolium Alexandrinum ◽  
Low Concentrations ◽  
Berseem Clover ◽  
Layer Chromatography

A thin layer chromatography technique was used to identify and semiquantitate the oestrogenic isoflavones in berseem clover (Trifolium alexandrinum). Biochanin A and genistein were the most prominent isoflavones in all the three cuts of fresh berseem clover. Low concentrations of formononetin and daidzein were also detected. The concentrations of the all oestrogenic isoflavones gradually decreased in the successive cuts. Fermentation of all three fresh cuts in silo or in rumen fluid increased the concentrations of these isoflavones. Incubation of the silage materials with rumen fluid had little effect on the concentrations of the isoflavones. The concentrations of the oestrogenic isoflavones in fresh or fermented materials considered were too small to have any adverse effects on growth or reproduction of farm animals.

Wether bioassay of animal pasture legumes. II. The oestrogenic activity of nine strains of Trifolium subterraneum L.

1964 ◽  
Vol 15 (4) ◽  
pp. 527 ◽  
Author(s):  
AJ Millington ◽  
CM Francis ◽  
NR McKeown
Keyword(s):  
Regression Analysis ◽  
Thin Layer ◽  
Multiple Regression Analysis ◽  
Positive Relationship ◽  
Subterranean Clover ◽  
Trifolium Subterraneum ◽  
The Other ◽  
Biochanin A ◽  
Pasture Legumes ◽  
Layer Chromatography

Nine strains of subterranean clover (Trifolium subterraneum L.) were assayed for oestrogenic activity with changes in wether teat length as the criterion. The isoflavone and coumestrol contents of each strain were determined by thin-layer chromatography. Significant differences were obtained between strains. Multiple regression analysis indicated a positive relationship between increase in teat length and the formononetin content of the strains. No such relationship was found for either genistein or biochanin A, the other isoflavones that were present in appreciable amounts.

Thin-Layer Chromatography

1965 ◽  
Author(s):  
H. R. Bolliger ◽  
M. Brenner ◽  
H. Gänshirt ◽  
Helmut K. Mangold ◽  
H. Seiler ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Layer Chromatography

THE LESS-POLAR METABOLITES PRODUCED BY INCUBATION OF TESTOSTERONE-4-14C WITH RAT AND BOVINE BRAIN

1969 ◽  
Vol 61 (4) ◽  
pp. 641-648 ◽  
Author(s):  
Leon J. Sholiton ◽  
Emile E. Werk
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Paper Chromatography ◽  
Specific Activity ◽  
Bovine Brain ◽  
Polar Fraction ◽  
End Products ◽  
Polar Metabolites ◽  
Layer Chromatography ◽  
Constant Specific Activity

ABSTRACT Rat and bovine brain have been incubated with testosterone-4-14C under standard conditions. With use of paper chromatography, the extracted metabolites were noted to fall into less-polar, iso-polar, and more polar fractions. The components of the less-polar fraction were separated by acetylation and thin-layer chromatography and the major end-products identified by recrystallization to constant specific activity or constant 3H/14C ratios. Androst-4-enedione and 5α-dihydrotestosterone were formed consistently under the conditions utilized. Trace amounts of other less-polar metabolites were noted occasionally.

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