Sulfur and methionine metabolism in sheep. III.* Excretion and retention of dietary and supplemented sulfur, and production responses to intraruminal infusions of DL-methionine

1979 ◽  
Vol 30 (6) ◽  
pp. 1185 ◽  
Author(s):  
PT Doyle ◽  
RJ Moir
Keyword(s):  
Amino Acid ◽  
Dry Matter ◽  
Dry Matter Intake ◽  
Methionine Metabolism ◽  
Sulfate Sulfur ◽  
Wool Growth ◽  
Methionine Supplementation ◽  
Different Levels ◽  
Basal Ration ◽  
Inorganic Sulfate

Two experiments were carried out to study the excretion and retention of nitrogen and sulfur when intraruminal infusions of DL-methionine were given to sheep. Merino wethers were fed on a 50:50 oaten chaff: lucerne chaff ration at different levels of dry matter intake (DMI), either as chaff or ground and pelleted, and with or without intraruminal infusions of DL-methionine. Liveweight gain in experiment 1, and the rate of wool growth and sulfur incorporation into wool were also measured. [35S]L-methionine was used to trace sulfur excretion from the supplement. Increasing the DMI increased (P < 0.001) nitrogen and sulfur excretion in both faeces and urine, but also increased (P < 0.001) the apparent retention of nitrogen and sulfur. The excretion of nitrogen and sulfur was not significantly affected by the form in which the basal ration was supplied, but sulfur retention did improve (P < 0.05) when the ration was given as chaff. DL-methionine supplements resulted in large increases (P < 0.001) in the excretion of total and inorganic sulfate sulfur in urine, and there was a tendency for urinary neutral sulfur excretion to increase. The amino acid supplement did not affect faecal sulfur excretion except at the lower level of feed intake in experiment 2. Differences in the amount of sulfur, from DL-methionine and [35S]L- methionine injections given into the rumen, excreted in faeces and urine indicate possible differences in the metabolism of the amino acid isomers. Increasing DMI resulted in improved (P < 0.001) rates of liveweight gain and wool growth; DL-methionine infusions had no effect. Further, estimates of sulfur incorporation into wool indicate that very little supplementary methionine was utilized at the follicular sites despite increased sulfur retention. The patterns of sulfur excretion suggest that the added methionine was rapidly and virtually completely dissimilated in the rumen. It is concluded that production responses to dietary methionine supplementation occur only under sulfur-deficient conditions or occasionally where degradation is avoided. _____________________ *Part II, Aust. J. Biol. Sci., 32: 65 (1979).

Effects of intraperitoneal injections of DL-methionine on the voluntary intake and wool growth of sheep fed sole diets of hay, silage and pasture differing in digestibility

1976 ◽  
Vol 86 (1) ◽  
pp. 141-149 ◽  
Author(s):  
T. N. Barry
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Dry Matter ◽  
Dry Matter Intake ◽  
Amino Acid Supplementation ◽  
High Quality ◽  
Wool Growth ◽  
Methionine Supplementation ◽  
Ad Libitum ◽  
Voluntary Intake

SUMMARYPenned Romney wether sheep were fed ad libitum diets of untreated silage and hay made from the same pasture (Expt 1), untreated silage (Expt 2), and high quality fresh pasture (Expt 3). Voluntary intake and wool growth were measured. In each experiment half of the sheep were given intraperitoneal injections three times per week which supplied the equivalent of 1·03 g DL-methionine/day.Relative to silage in Expt 1, hay increased voluntary dry matter intake, depressed apparent nitrogen digestibility and increased wool growth. Methionine supplementation increased voluntary intake and wool growth with animals fed on hay or silage in Expt 1 and with those fed on silage in Expt 2, but had no effect on either variate with the diet of fresh pasture.Wool sulphur content, measured in Expts 2 and 3, was increased by methionine supplementation, and it was calculated that 34 and 15% respectively of the sulphur administered as methionine was recovered in the wool.The effects produced by methionine supplementation in this work are compared with those produced from different forms of post-ruminal amino acid supplementation in sheep fed a variety of diets. Factorial estimates of the requirements for sulphur of sheep and cattle in different physiological states have been calculated, and are discussed in relation to results of trials involving supplementation with sulphur-containing amino acids.

scholarly journals THE EFFECTS OF FEED ON THE INTAKE, DIGESTIBILITY AND GROWTH RATE OF WEST AFRICAN DWARF GOATS FED GLIRICIDIA sepium AND LEUCAENA LEUCOCOEPHALA

2021 ◽  
Vol 22 (2) ◽  
pp. 164-168
Author(s):  
S. M. Odeyinka ◽  
A. A. Ademosun
Keyword(s):  
Growth Rate ◽  
Dry Matter ◽  
Gliricidia Sepium ◽  
West African ◽  
Dry Matter Intake ◽  
West African Dwarf ◽  
West African Dwarf Goats ◽  
Significant Difference ◽  
Different Levels ◽  
Dwarf Goats

The study evaluated the effects of level of feed offer on dry matter intake (DMI) , digestibility, the parts of plants selected and on the growth rate of West African dwarf (WAD) goats without resulting in toxicity. Seven different levels of feed offer ranging from 40g/kg0.75/day to 130Wkg0.75/day in increments of 15g/kg0.75) were fed to two groups of goats. The first group was fed 100% Gliricidia while the while the second was fed 50% Gliricidia and 50% Leucaena diet (50/50). The level of feed offer had significant effect on the DIM and digestible DMI (DDMI) of the goat (P < 0.01). The Leucaena intake in a 50/50 diet was always higher than the Gliricidia intake except at 55g1kg0.75 offer level. The level of feed offer had significant effects on the parts of plants selected (P < 0.01.) and on the growth rate. The leaf intake was statistically higher than the stem intake (P < 0.01). All the goats on 40g/kg0.75 and 55g/kg0.75 on a 100% Gliricidia diet lost weight while only those on 40g/kg0.75 lost weight on a 50/50 diet. There was no significant difference in the growth rate of goats on feed offer levels of 70g to 130g/kg0.75 (P > 0.05). The study indicate that goats performance was higher on a mixed browse diet than on Gliricidia alone. Goats performed best at 100g/kg0.75 offer level.

Estimation of intake and digestibility of forage-based diets in group-fed dairy cows using alkanes as markers

1999 ◽  
Vol 133 (4) ◽  
pp. 419-425 ◽  
Author(s):  
Y. UNAL ◽  
P. C. GARNSWORTHY
Keyword(s):  
Dairy Cows ◽  
Milk Production ◽  
Dry Matter ◽  
Dry Matter Intake ◽  
Once Daily ◽  
Major Factors ◽  
Filter Papers ◽  
Selection Of ◽  
Basal Ration ◽  
Food Consumed

Dry matter intake is one of the major factors limiting milk production in dairy cows, although the quantity of food consumed by an individual cow when housed and fed as part of a group is rarely known. Such information would permit more precise ration formulation, concentrate allocation and selection of cows according to efficiency of milk production. Alkanes have been used with sheep and cattle to estimate feed intake under grazing conditions and could provide a technique for measuring intake in housed dairy cows. The purpose of this study was to examine alkanes C32 and C36, in combination with alkane C33, as indigestible markers for estimating intake of housed dairy cows fed on different diets under experimental and commercial conditions. Three experiments were conducted with hay-based diets (Expt 1), silage only diets (Expt 2) and a diet consisting of a silage-based basal ration plus concentrates (Expt 3). Animals were dosed once daily with C32 and C36, either on filter papers (Expts 1 and 2) or as part of a specially prepared concentrate (Expt 3). Faecal recoveries of alkanes ranged from 0·88 to 0·99. Over the range of intakes found in the three experiments (6–24 kg DM/d), the r2 values for estimated versus actual dry matter intakes ranged from 0·81 to 0·99. It is concluded that alkanes could provide a useful technique for estimating intake in dairy cows housed and fed in groups.

Effects of feeding different levels of chromium-enriched live yeast in hairy lambs fed a corn-based diet: effects on growth performance, dietary energetics, carcass traits and visceral organ mass

2013 ◽  
Vol 53 (4) ◽  
pp. 308 ◽  
Author(s):  
A. Estrada-Angulo ◽  
Y. S. Valdés ◽  
O. Carrillo-Muro ◽  
B. I. Castro-Perez ◽  
A. Barreras ◽  
...  
Keyword(s):  
Growth Performance ◽  
Dry Matter ◽  
Carcass Traits ◽  
Dry Matter Intake ◽  
Longissimus Muscle ◽  
Visceral Organ ◽  
Muscle Area ◽  
Organ Mass ◽  
Different Levels ◽  
Live Yeast

Forty Pelibuey × Kathdin lambs (35.5 ± 0.4 kg) were used in a 56-day feeding experiment to assess the effects of feeding different levels of chromium-enriched live yeast (Cr-YC) on growth performance, dietary energetics, carcass traits and visceral organ mass. The Cr-YC source contained 5.5 × 109 colony forming units (CFU) and 0.40 mg of Cr per gram. Treatments consisted of a dry rolled corn-based finishing diet supplemented with 0, 1, 2 or 3 g Cr-YC/lamb.day. Total daily dosages were: 5.5 × 109 CFU and 0.4 mg; 1.1 × 1010 CFU and 0.8 mg Cr, and 1.65 × 1010 CFU and 1.2 mg Cr for supplementation levels of 1, 2 or 3 g Cr-YC/lamb.day, respectively. There were no treatments effects on dry matter intake. As the level of Cr-YC supplementation increased, average daily gain, gain to feed and dietary net energy were linearly increased, and observed/expected dry matter intake was linearly decreased. Chromium-enriched live yeast supplementation increased empty bodyweight (EBW), gastrointestinal fill and full viscera weight, but did not influence organ weights as a proportion of EBW (g/kg EBW). Cr-YC level did not affect carcass length, backfat thickness, kidney, pelvic and heart fat or body wall thickness, but increased hot carcass weight and longissimus muscle area. In general, treatment effects on percentage yield of wholesale cuts (tissue weight as a percentage of cold carcass weight) were small. However, Cr-YC decreased percentage flank. Chromium-enriched yeast supplementation enhances growth rate, longissimus muscle area, and dietary energetic efficiency in finishing feedlot lambs.

scholarly journals Sulfur and Methionine Metabolism in Sheep. IV. Metabolism and Absorption in the Intestines

1980 ◽  
Vol 33 (3) ◽  
pp. 303 ◽  
Author(s):  
PT Doyle ◽  
RJ Moir
Keyword(s):  
Dry Matter ◽  
Dry Matter Intake ◽  
Methionine Metabolism ◽  
Faecal Excretion ◽  
Pelleted Diet

Twelve I8-month-old Merino wethers were fed a ground and pelleted diet of equal parts oaten chaff and lucerne chaff. Sulfur flows at the ileum and faecal excretion of sulfur were estimated for two levels of dry matter intake (500 and 1000 g/day) in sheep receiving intraruminal infusions of water or 4�5 g DL-methionine per day.

Effect of added dietary nitrate and elemental sulfur on wool growth and methane emission of Merino lambs

2013 ◽  
Vol 53 (11) ◽  
pp. 1195 ◽  
Author(s):  
L. Li ◽  
C. I. Silveira ◽  
J. V. Nolan ◽  
I. R. Godwin ◽  
R. A. Leng ◽  
...  
Keyword(s):  
Methane Emission ◽  
Methane Production ◽  
Dry Matter ◽  
Elemental Sulfur ◽  
Methane Yield ◽  
Dry Matter Intake ◽  
Feed Conversion ◽  
Dietary Nitrate ◽  
Wool Growth ◽  
Nutrient Utilisation

The effects of dietary nitrate (NO3) and elemental sulfur (S) on nutrient utilisation, productivity, and methane emission of Merino lambs were investigated. Forty-four lambs were randomly allocated to four groups (n = 11) fed isonitrogenous and isoenergetic diets. The basal feed was supplemented with 1% urea + 0.18% S (T1), 1.88% NO3 + 0% S (T2), 1.88% NO3 + 0.18% S (T3), or 1.88% NO3 + 0.40% S (T4). Retention of S was improved by increasing the content of elemental S in the NO3-containing diet (P < 0.001), yet the N retention (g/day) by the animal, and the N and S content of wool (%), were not altered by S supplementation (P > 0.05). Dry matter intake, liveweight gain, and feed conversion ratio did not differ (P > 0.05) between treatments. Replacing urea with NO3 improved the rate of clean wool growth by 37% (P < 0.001, T1 vs T3). Clean wool growth increased by 26% (P < 0.001) when the S content of the NO3-containing diet was increased from 0 to 0.18% (T2 vs T3). Methane production (g/day) and methane yield (g/kg DM intake) were reduced (P < 0.05) by 24% when urea was replaced by NO3 (T1 vs T3). The addition of 0.4% S to a diet containing 1.88% NO3 also reduced methane production (P = 0.021) and methane yield (P = 0.028). In conclusion, the addition of 1.88% NO3 and 0.18% elemental S to a total mixed diet increased clean wool production and reduced methane production. However, there was no evidence of inter-relationships between NO3 and S.

Effect of post-ruminal infusion of glutamine on plasma amino acids, milk yield and composition in lactating dairy cows

2001 ◽  
Vol 81 (2) ◽  
pp. 229-235 ◽  
Author(s):  
J. C. Plaizier ◽  
J. -P. Walton ◽  
B. W. McBride
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Blood Plasma ◽  
Dairy Cows ◽  
Milk Yield ◽  
Dry Matter ◽  
Milk Composition ◽  
Plasma Amino Acid ◽  
Dry Matter Intake ◽  
Plasma Amino Acids

The objectives of this study were to examine the effect of supplying post-ruminal L-glutamine in mid-lactation Holstein dairy cows on plasma amino acid profile, dry matter intake, milk yield and milk composition. The experiment was designed as a 4 × 4 Latin square with four 2-wk periods. Cows were continuously infused post-ruminally with graded levels of L-glutamine (L-Gln) for 5 consecutive days during the second week of each period. During the last 24 h of the infusion, blood plasma was collected every 6 h for the determination of plasma amino acid concentrations. Plasma Gln concentration increased (P < 0.05) with increasing L-Gln daily infusions. Infusion of 0, 100, 200, and 300 g d–1 resulted in blood plasma Gln concentrations of 289.5, 299.2, 356.4 and 386.2 mmol L–1, respectively. The level of Gln infusion also resulted in a decrease in the blood plasma concentration of phenylalanine, but the concentrations of all other amino acids were not affected. The administration of Gln did not affect dry matter intake, milk yield and milk composition. Key words: Post-ruminal, L-glutamine, plasma amino acids, milk constituents

Use of dietary nitrate to increase productivity and reduce methane production of defaunated and faunated lambs consuming protein-deficient chaff

2016 ◽  
Vol 56 (3) ◽  
pp. 290 ◽  
Author(s):  
S. H. Nguyen ◽  
M. C. Barnett ◽  
R. S. Hegarty
Keyword(s):  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Average Daily Gain ◽  
Dry Matter Intake ◽  
Microbial Protein ◽  
Molar Percentage ◽  
Ch4 Production ◽  
Wool Growth ◽  
Nitrate Supplementation ◽  
Daily Gain

The effects of dietary nitrate supplementation and defaunation on methane (CH4) emission, microbial protein outflow, digesta kinetics and average daily gain were studied in lambs fed chaff containing 4.1% crude protein in dry matter. Twenty ewe lambs were randomly allocated in a 2 × 2 factorial experiment (0% or 3.1% calcium nitrate supplementation and defaunated or faunated protozoal state). Nitrate supplementation increased blood methaemoglobin concentration (P < 0.05), rumen volatile fatty acids, ammonia concentration, dry matter intake, microbial protein outflow, average daily gain, dry matter digestibility, clean wool growth and wool fibre diameter (P < 0.01). Nitrate increased CH4 production (g/day) due to greater dry matter intake, but did not affect CH4 yield (g/kg dry matter intake). Nitrate-supplemented lambs had a shorter total mean retention time of digesta in the gut (P < 0.05). Defaunation reduced CH4 production and CH4 yield by 43% and 47%, but did not cause changes in dry matter intake, microbial protein outflow, average daily gain or clean wool growth. Defaunation decreased total volatile fatty acids and the molar percentage of propionate, but increased the molar percentage of acetate (P < 0.05). Interactions were observed such that combined treatments of defaunation and nitrate supplementation increased blood methaemoglobin (P = 0.04), and decreased CH4 yield (P = 0.01).

scholarly journals RETENSI N, Ca DAN P PADA KAMBING YANG DIBERI DEDAK HALUS YANG DIAUTOCLAVE DENGAN LEVEL BERBEDA

ZOOTEC ◽  
2015 ◽  
Vol 35 (1) ◽  
pp. 86
Author(s):  
E Pudjihastuti
Keyword(s):  
Body Weight ◽  
Experimental Method ◽  
Treatment Effect ◽  
Dry Matter ◽  
Latin Square ◽  
Dry Matter Intake ◽  
Test Analysis ◽  
Different Levels ◽  
Brachiaria Mutica ◽  
Range Test

RETENTION OF N, Ca and P in GOAT GIVEN FINE BRAN THAT OUTOCLAVED WITH DIFFERENT LEVEL. The aim of this study was to determine the effect of autoclaved fine bran use at different levels on the retention of nitrogen (N), calcium (Ca) and phosphorus (P) in goats. This study used four goats at age of 7-8 months old with body weight of 8-10 kg. Basic feed used was Brachiaria mutica grass, which added with autoclaved fine bran at different levels, as follows: R0 = Brachiaria mutica grass R1 = Brachiaria mutica grass+ 10% autoclaved fine bran R2 = Brachiaria mutica grass+ 20% autoclaved fine bran R1 = Brachiaria mutica grass+ 30% autoclaved fine bran This study used an experimental method based on the 4 x 4 Latin square design, where livestock served as row and experimental method as a line, followed by Duncan's multiple range test analysis. Results of analysis of variance showed that the treatment effect is highly significant (p <0.01) on dry matter intake, consumption and retention of N, consumption and retention of Ca and P. With Duncan's multiple range test showed that the treatment R3 was higher (p <0.01) than treatments of R2, R1 and R0 on consumption and retention of N, Ca and P. From these results, it can be concluded that the use of autoclaved fine bran up to 30% can improve the retention of N, Ca and P in goats.   Keywords: Fine bran, Autoclave, Retention, Goat

scholarly journals Feed Intake and Nutrient Utilization by Three Breeds of Cattle

2021 ◽  
Vol 8 (1) ◽  
pp. 57-66
Author(s):  
U. J. Ikhatua ◽  
F. O. Olubajo
Keyword(s):  
Feed Intake ◽  
Dry Matter ◽  
Nutrient Utilization ◽  
Metabolizable Energy ◽  
Dry Matter Intake ◽  
Groundnut Cake ◽  
Exotic Animals ◽  
Cynodon Nlemfuensis ◽  
Basal Ration

Two feeding and digestibility trials were carried out to investigate the effects of groundnut cake (GNC) supplementation on feed intake, DM digestibility, nutrient utilization and liveweight gain of three breeds of cattle fed on all-roughage rations (hay and fresh grass) of Cynodon nlemfuensis var robustus. The results showed a decrease in mean DM intake of the basal ration by approximately 13.14%; 1.9% and 6.4% and an increase in total dry matter intake (basal + GNC supplement) of approximately 0.42%), 11.56% and 11.04% for the indigenous, crosses and exotic animals respectively. The results also showed non-significent increases in the apparent digestibilities of the nutrients. Both the total Digestible Nutrients (TDN) kg/W0.75 and Metabolizable Energy (ME) Kcal/W0.75 intake by the three breeds of cattle were increased with supplementation and mean daily liveweight increased by 18.92%, 13.15% and 16.27% respectively.

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