Selection against sprouting damage in wheat. III.* Dormancy, germinative alpha-amylase, grain redness and flavanols

1979 ◽  
Vol 30 (3) ◽  
pp. 387 ◽  
Author(s):  
IL Gordon
Keyword(s):  
The Other ◽  
Grain Development ◽  
Germination Inhibitors ◽  
Development Patterns ◽  
White Wheat ◽  
Long Period ◽  
Short Period ◽  
Embryo Dormancy ◽  
Different Levels

Grain development of embryo dormancy, germinative α-amylase, pigmentation and flavanols was examined in the wheats Timgalen and Gamut (white-grained, non-dormant), and Pembina and Sonora (red-grained with different levels of dormancy). It was found that each trait had distinctive patterns of development. The net result at harvest ripeness depended on the synchronizations amongst the traits. Dormancy, as judged by embryo response (i.e. embryo dormancy), was restricted to the red wheats. Three ways of expressing it were noted: (1) in terms of development patterns, (2) as levels at harvest ripeness or at harvest, and (3) by the length of the period of embryo dormancy after harvest ripeness. Two hypotheses linking embryo dormancy and grain redness appeared plausible from the results. One was that the polyphenol oxidase complex, which polymerizes flavanols to the putative pigment phlobaphene, contributes towards embryo dormancy, probably through enhancement of hypo-oxia. The other was that the pigment itself and its tanning complexes cause the hypo-oxia. Flavanols did not appear to be in vivo germination inhibitors. Dormancy, as judged by α-amylase response (i.e. amylase dormancy), was not always present together with embryo dormancy. A long period of amylase dormancy was found in the more embryo-dormant red wheat, but not in the other. Conversely, a short period of amylase dormancy was found in one white wheat, but it was not embryo-dormant. Possible relationships between these physiological traits and the classical genes for red grain-coat were discussed. Implications concerning selection against sprouting damage were considered. _________________ *Part II, Aust. J. Agric. Res 30: 1-17 (1979).

scholarly journals Drosophila DBT Lacking Protein Kinase Activity Produces Long-Period and Arrhythmic Circadian Behavioral and Molecular Rhythms

2007 ◽  
Vol 27 (23) ◽  
pp. 8049-8064 ◽  
Author(s):  
Michael J. Muskus ◽  
Fabian Preuss ◽  
Jin-Yuan Fan ◽  
Edward S. Bjes ◽  
Jeffrey L. Price
Keyword(s):  
Kinase Activity ◽  
Protein Kinase Activity ◽  
Wild Type ◽  
Endogenous Gene ◽  
Long Period ◽  
Short Period ◽  
Lower Expression ◽  
General Reduction

ABSTRACT A mutation (K38R) which specifically eliminates kinase activity was created in the Drosophila melanogaster ckI gene (doubletime [dbt]). In vitro, DBT protein carrying the K38R mutation (DBTK/R) interacted with Period protein (PER) but lacked kinase activity. In cell culture and in flies, DBTK/R antagonized the phosphorylation and degradation of PER, and it damped the oscillation of PER in vivo. Overexpression of short-period, long-period, or wild-type DBT in flies produced the same circadian periods produced by the corresponding alleles of the endogenous gene. These mutations therefore dictate an altered “set point” for period length that is not altered by overexpression. Overexpression of the DBTK/R produced effects proportional to the titration of endogenous DBT, with long circadian periods at lower expression levels and arrhythmicity at higher levels. This first analysis of adult flies with a virtual lack of DBT activity demonstrates that DBT's kinase activity is necessary for normal circadian rhythms and that a general reduction of DBT kinase activity does not produce short periods.

scholarly journals Trypanosoma cruzi: chemotherapy with benznidazole in mice inoculated with strains from Paraná State and from different endemic areas of Brazil

1997 ◽  
Vol 39 (5) ◽  
pp. 283-290 ◽  
Author(s):  
Max Jean de Ornelas TOLEDO ◽  
Ana Lúcia Falavigna GUILHERME ◽  
José Carlos da SILVA ◽  
Marco Venício de GASPERI ◽  
Andréa Pereira MENDES ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Drug Susceptibility ◽  
The Other ◽  
Inflammatory Lesions ◽  
Triatoma Sordida ◽  
Northwestern Region ◽  
Endemic Areas ◽  
Different Levels ◽  
Trypanocidal Drugs

Strains of Trypanosoma cruzi from different geographical areas have shown different levels of susceptibility to trypanocidal drugs. The susceptibility in vivo to benznidazole was investigated in eighteen strains of T. cruzi. Twelve were isolated from chronic chagasic patients from different Chagas’ disease endemic areas. The other six strains were isolated from the northwestern region of Paraná state; two of them from patients three from triatomines (Triatoma sordida) and one from wild reservoir (Didelphis sp.). To test drug the infected mice were divided into two groups of twenty. One group was treated with benznidazole for twenty consecutive days and the other group was used as untreated control. The treatment began after detection of the infection by direct blood examination or haemoculture. The control of cure was done through haemoculture and indirect immunofluorescence test. The drug eliminated the inflammatory lesions of the skeletal muscle of mice considered cured and from the heart of most of them. Moreover, the inflammatory lesions were reduced in treated but not cured animals. The T. cruzi strains studied showed a gradient of drug susceptibility that varied from 0% to 100%. Ten strains were considered sensitive to the treatment (61 to 100% of cure), one strain was partially sensitive (50% of cure) and seven strains were considered resistant to the treatment (0 to 40% of cure). This variation was observed both in strains of T. cruzi isolated from domestic and sylvatic cycles

Religion et société dans 1'histoire de 1'Espagne

1986 ◽  
Vol 33 (4) ◽  
pp. 363-383
Author(s):  
Fernando Urbina ◽  
José Sanchez
Keyword(s):  
Civil War ◽  
Transition To Democracy ◽  
The Other ◽  
Modern History ◽  
Old Regime ◽  
Long Period ◽  
Historic Time ◽  
Short Period ◽  
The Subject

This work is a collaboration between José Sánchez and Fer nando Urbina. José Sdnchez is the author of the first part, which analizes the relation between catholicism and society in the « short period» of modern history (Centuries XIX and XX) -conflictive period, during which Spain enters modernism, from the fall of the old regime up to the civil war and the transition to democracy-, Fernando Urbina is responsible for the second part which traces an outline on the « long period» of popular religiousness. Both have collaborated in the reasoning and resolution of the subject; presenting the general diachronic frame of historic time, in which the most synchronic and present arguments of the other articles within this piece, must be situated.

Immunologie Studies in von Willebrand’s Disease

1976 ◽  
Vol 35 (01) ◽  
pp. 110-119 ◽  
Author(s):  
Y Sultan ◽  
J Simeon ◽  
P Maisonneuve ◽  
J. P Caen
Keyword(s):  
Factor Viii ◽  
Bleeding Time ◽  
The Other ◽  
Von Willebrand's Disease ◽  
Factor Viii Related Antigen ◽  
Von Willebrand’S Disease ◽  
Short Period ◽  
Von Willebrand ◽  
Immunologic Properties

SummaryTwo patients with a severe von Willebrand’s disease characterized by no detectable factor VIII related antigen in their plasma received transfusions of cryoprecipitate. The bleeding time was corrected for a short period of time and returned to its pretransfusional value although the other parameters of the disease were still corrected. Electrophoretic and immunologic properties of factor VIII related antigen infused were determined serially after transfusion. Modifications of these properties occurred progressively after transfusion. The half disappearance time of F. VIIIR. A. was determined and found to be considerably shorter than in hemophilic recipients. This study suggests an alteration in vivo of F. VIIIR. A. infused into von Willebrand recipients.

THE EFFECT OF LOCAL TEMPERATURE ON THE REACTIVITY TO NORADRENALINE OF DIGITAL VESSELS

1967 ◽  
Vol 45 (1) ◽  
pp. 93-102 ◽  
Author(s):  
Peter Gaskell ◽  
David L. Hoeppner
Keyword(s):  
Intravenous Infusion ◽  
The Other ◽  
Local Temperature ◽  
Continuous Intravenous Infusion ◽  
Opening Pressure ◽  
Simultaneous Measurements ◽  
Critical Opening ◽  
Short Period ◽  
The Mean

The effect of local temperature on the in vivo reactivity of vascular smooth muscle was studied. Reactivity was measured as the increase in critical opening pressure (COP) of digital vessels caused by intravenous infusion of 5 μg of noradrenaline per minute. With one hand cool (22 °C) and the other warm (34 °C) in test experiments or both hands either cool or warm in control experiments, simultaneous measurements were made of the increase in COP of vessels in both middle fingers in response to the noradrenaline. In control experiments the mean increase in COP was similar in right and left fingers, but in test experiments the mean increase was greater in the warm finger than in the cool one. Warm vessels were more reactive to noradrenaline than cool ones (p < 0.01). Because a short period of ischemia is involved in the measurement of COP, other experiments were performed in which the effect of duration of ischemia on the COP, with and without a continuous intravenous infusion of noradrenaline, was ascertained. They suggested that the estimated COP would, in most cases, be about 3 mm Hg less than the COP existing just before the measurement. These results also indicated that although the rate of fall of COP during ischemia was slightly greater for a higher initial COP, the ischemia involved did not invalidate the comparison of the increases in COP caused by noradrenaline in warm and cool fingers, as an index of relative arteriolar reactivity in the test reactivity experiments.

scholarly journals Impact of Tigecycline Versus Other Antibiotics on the Fecal Metabolome and on Colonization Resistance to Clostridium difficile in Mice

2017 ◽  
Vol 2 (1) ◽  
pp. 1 ◽  
Author(s):  
Robin L.P. Jump ◽  
David Kraft ◽  
Kelly Hurless ◽  
Alex Polinkovsky ◽  
Curtis J. Donskey
Keyword(s):  
Clostridium Difficile ◽  
Intestinal Microbiota ◽  
High Performance ◽  
The Other ◽  
Colonization Resistance ◽  
Bacterial Metabolites ◽  
Metabolic Functions ◽  
Short Period ◽  
Lipid Metabolites

Background: The glycylcycline antibiotic tigecycline may have a relatively low propensity to promote Clostridium difficile infection in part because it causes less disruption of the indigenous intestinal microbiota than other broad-spectrum antibiotics.  We used a mouse model to compare the compare the effects of tigecycline versus other commonly used antibiotics on colonization resistance to C. difficile and on metabolic functions of the intestinal microbiota.   Methods: To assess in vivo colonization resistance to C. difficile, mice were challenged with oral C. difficile spores 1, 7, or 12 days after completion of 3 days of treatment with subcutaneous saline, tigecycline, ceftriaxone, piperacillin-tazobactam, or linezolid.  Levels of bacterial metabolites in fecal specimens of mice treated with the same antibiotics were analyzed using non-targeted metabolic profiling by gas chromatograph (GC)/mass spectrometry (MS) and ultra-high performance liquid chromatography-tandem MS (UPLC-MS/MS).  Results:  All of the antibiotics disrupted colonization resistance to C. difficile when challenge occurred 2 days after treatment.  Only piperacillin/tazobactam and ceftriaxone-treated mice had disturbed colonization resistance at 7 days after treatment.  All of the antibiotics altered fecal metabolites in comparison to controls, but tigecycline caused significantly less alteration than the other antibiotics, including less suppression of multiple amino acids, bile acids, and lipid metabolites.   Conclusions:  Tigecycline and linezolid caused transient disruption of colonization resistance to C. difficile, whereas ceftriaxone and piperacillin/tazobactam caused disruption that persisted for 7 days post-treatment.  Tigecycline caused less profound alteration of fecal bacterial metabolites than the other antibiotics, suggesting that the relatively short period of disruption of colonization resistance might be related in part to reduced alteration of the metabolic functions of the microbiota

Discussion following the presentation by F. Deubner

1977 ◽  
Vol 36 ◽  
pp. 69-74
Keyword(s):  
List Type ◽  
The Sun ◽  
Type Number ◽  
Harmonic Motion ◽  
Locally Excited ◽  
Long Period ◽  
Coherent Wave ◽  
Short Period ◽  
Global Modes

The discussion was separated into 3 different topics according to the separation made by the reviewer between the different periods of waves observed in the sun :1) global modes (long period oscillations) with predominantly radial harmonic motion.2) modes with large coherent - wave systems but not necessarily global excitation (300 s oscillation).3) locally excited - short period waves.

Enhancement of ADP-induced Platelet Aggregation by Adrenaline in Vivo and Its Prevention

1973 ◽  
Vol 29 (02) ◽  
pp. 490-498 ◽  
Author(s):  
Hiroh Yamazaki ◽  
Itsuro Kobayashi ◽  
Tadahiro Sano ◽  
Takio Shimamoto
Keyword(s):  
Platelet Count ◽  
Platelet Aggregation ◽  
Platelet Rich Plasma ◽  
The Other ◽  
Endothelial Surface ◽  
Important Interaction ◽  
Blood Coagulability ◽  
The Difference

SummaryThe authors previously reported a transient decrease in adhesive platelet count and an enhancement of blood coagulability after administration of a small amount of adrenaline (0.1-1 µg per Kg, i. v.) in man and rabbit. In such circumstances, the sensitivity of platelets to aggregation induced by ADP was studied by an optical density method. Five minutes after i. v. injection of 1 µg per Kg of adrenaline in 10 rabbits, intensity of platelet aggregation increased to 115.1 ± 4.9% (mean ± S. E.) by 10∼5 molar, 121.8 ± 7.8% by 3 × 10-6 molar and 129.4 ± 12.8% of the value before the injection by 10”6 molar ADP. The difference was statistically significant (P<0.01-0.05). The above change was not observed in each group of rabbits injected with saline, 1 µg per Kg of 1-noradrenaline or 0.1 and 10 µg per Kg of adrenaline. Also, it was prevented by oral administration of 10 mg per Kg of phenoxybenzamine or propranolol or aspirin or pyridinolcarbamate 3 hours before the challenge. On the other hand, the enhancement of ADP-induced platelet aggregation was not observed in vitro, when 10-5 or 3 × 10-6 molar and 129.4 ± 12.8% of the value before 10∼6 molar ADP was added to citrated platelet rich plasma (CPRP) of rabbit after incubation at 37°C for 30 second with 0.01, 0.1, 1, 10 or 100 µg per ml of adrenaline or noradrenaline. These results suggest an important interaction between endothelial surface and platelets in connection with the enhancement of ADP-induced platelet aggregation by adrenaline in vivo.

Significance of In-Vitro and In-Vivo Correlation in Drug Delivery System

2018 ◽  
Vol 4 (4) ◽  
pp. 523-531
Author(s):  
Hina Mumtaz ◽  
Muhammad Asim Farooq ◽  
Zainab Batool ◽  
Anam Ahsan ◽  
Ashikujaman Syed
Keyword(s):  
Dosage Form ◽  
Pharmaceutical Preparations ◽  
Pharmacokinetic Profile ◽  
In Vitro Dissolution ◽  
Time Saving ◽  
Pharmaceutical Dosage Form ◽  
Short Period ◽  
Form Development

The main purpose of development pharmaceutical dosage form is to find out the in vivo and in vitro behavior of dosage form. This challenge is overcome by implementation of in-vivo and in-vitro correlation. Application of this technique is economical and time saving in dosage form development. It shortens the period of development dosage form as well as improves product quality. IVIVC reduce the experimental study on human because IVIVC involves the in vivo relevant media utilization in vitro specifications. The key goal of IVIVC is to serve as alternate for in vivo bioavailability studies and serve as justification for bio waivers. IVIVC follows the specifications and relevant quality control parameters that lead to improvement in pharmaceutical dosage form development in short period of time. Recently in-vivo in-vitro correlation (IVIVC) has found application to predict the pharmacokinetic behaviour of pharmaceutical preparations. It has emerged as a reliable tool to find the mode of absorption of several dosage forms. It is used to correlate the in-vitro dissolution with in vivo pharmacokinetic profile. IVIVC made use to predict the bioavailability of the drug of particular dosage form. IVIVC is satisfactory for the therapeutic release profile specifications of the formulation. IVIVC model has capability to predict plasma drug concentration from in vitro dissolution media.

Oxytocin analogues with non-coded amino acid residues in position 8: [8-Neopentylglycine]oxytocin and [8-cycloleucine]oxytocin

1987 ◽  
Vol 52 (9) ◽  
pp. 2317-2325 ◽  
Author(s):  
Jan Hlaváček ◽  
Jan Pospíšek ◽  
Jiřina Slaninová ◽  
Walter Y. Chan ◽  
Victor J. Hruby
Keyword(s):  
Amino Acid ◽  
Solid Phase Synthesis ◽  
Solid Phase ◽  
The Other ◽  
Amino Acid Residues ◽  
Phase Synthesis ◽  
Other Hand ◽  
Fragment Condensation

[8-Neopentylglycine]oxytocin (II) and [8-cycloleucine]oxytocin (III) were prepared by a combination of solid-phase synthesis and fragment condensation. Both analogues exhibited decreased uterotonic potency in vitro, each being about 15-30% that of oxytocin. Analogue II also displayed similarly decreased uterotonic potency in vivo and galactogogic potency. On the other hand, analogue III exhibited almost the same potency as oxytocin in the uterotonic assay in vivo and in the galactogogic assay.

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