Nodulation and growth of Medicago truncatula on acid soils. I. Effect of calcium carbonate and inoculation level on the nodulation of Medicago truncatula on a moderately acid soil

1970 ◽  
Vol 21 (3) ◽  
pp. 427 ◽  
Author(s):  
AD Robson ◽  
JF Loneragan
Keyword(s):  
Calcium Carbonate ◽  
Medicago Truncatula ◽  
Soil Ph ◽  
Soil Acidity ◽  
Rhizobium Meliloti ◽  
Acid Soil ◽  
Acid Soils ◽  
Acid Tolerant ◽  
Annual Medicago ◽  
Medicago Species

On a moderately acid soil (pH 4.6 in 115 suspension of soil in 0.01M calcium chloride), nodulation of Medicago truncatula cv. Cyprus responded markedly to increasing applications of calcium carbonate, which increased soil pH. Since the effect of increasing soil pH on the percentage nodulation could be replaced to a large extent by increasing the inoculation level, it appeared that nodulation was restricted by the inability of Rhizobium meliloti to survive or multiply in the acid soil. The growth of R, meliloti appeared more sensitive to soil acidity than growth of the host plant of annual Medicago species. It is suggested that more acid-tolerant strains of R. meliloti would permit annual Medicago species to be grown successfully on moderately acid soils, thus extending the range of soils suitable for the growth of these species.

Nodulation and growth of Medicago truncatula on acid soils. II. Colonization of acid soils by Rhizobium meliloti

1970 ◽  
Vol 21 (3) ◽  
pp. 435 ◽  
Author(s):  
AD Robson ◽  
JF Loneragan
Keyword(s):  
Calcium Carbonate ◽  
Medicago Truncatula ◽  
Rhizobium Meliloti ◽  
Acid Soils ◽  
Sandy Soils ◽  
Acid Tolerant ◽  
Second Year ◽  
Annual Medicago ◽  
Medicago Species ◽  
Better Than

Six strains of Rhizobium meliloti varied greatly in their ability to colonize two moderately acid sandy soils (pH 5.0-5.1 in 115 suspension of soil in 0.01M calcium chloride) in the year of sowing inoculated lime-coated seeds of Medicago truncatula. The strains also varied in their ability to nodulate M. truncatula in the year after sowing. With all rhizobial strains and on both soils, calcium carbonate applied in the year of sowing strongly promoted colonization in the year of sowing. It also greatly increased nodulation and growth of M. truncatula in the year after sowing. Apparently calcium carbonate increased second year nodulation by promoting the colonization of the acid soils by R. meliloti in the year of sowing. Differences among strains in second year nodulation appeared to be related to differences in the ability of the strains to colonize the soils in the year of sowing. Strains isolated from moderately acid sandy soils were markedly superior in both properties, and on both soils, to strain SU47 from commercial inoculum, but were generally no better than strain U45 from commercial inoculum. Even the most acid-tolerant strains colonized the acid soils relatively slowly. It is suggested that R. meliloti strains in general have a poor ability to colonize acid soils and that this property is a major factor limiting the growth and persistence of annual Medicago species on acid soils in the field.

EFFECTS OF SOIL ACIDITY ON RHIZOBIA NUMBERS, NODULATION AND NITROGEN FIXATION BY ALFALFA AND RED CLOVER

1977 ◽  
Vol 57 (2) ◽  
pp. 197-203 ◽  
Author(s):  
W. A. RICE ◽  
D. C. PENNEY ◽  
M. NYBORG
Keyword(s):  
Nitrogen Fixation ◽  
Soil Ph ◽  
Soil Acidity ◽  
Field Experiments ◽  
Rhizobium Meliloti ◽  
Acid Soils ◽  
Red Clover ◽  
Ion Concentration ◽  
Hydrogen Ion Concentration ◽  
Greenhouse Experiments

The effects of soil acidity on nitrogen fixation by alfalfa (Medicago sativa L.) and red clover (Trifolium pratense L.) were investigated in field experiments at 28 locations, and in greenhouse experiments using soils from these locations. The pH of the soils (limed and unlimed) varied from 4.5 to 7.2. Rhizobia populations in the soil, nodulation, and relative forage yields (yield without N/yield with N) were measured in both the field and greenhouse experiments. Rhizobium meliloti numbers, nodulation scores, and relative yields of alfalfa decreased sharply as the pH of the soils decreased below 6.0. For soils with pH 6.0 or greater, there was very little effect of pH on any of the above factors for alfalfa. Soil pH in the range studied had no effect on nodulation scores and relative yields of red clover. However, R. trifolii numbers were reduced when the pH of the soil was less than 4.9. These results demonstrate that hydrogen ion concentration is an important factor limiting alfalfa growth on acid soils of Alberta and northeastern British Columbia, but it is less important for red clover. This supports the continued use of measurements of soil pH, as well as plant-available Al and Mn for predicting crop response to lime.

AN ASSESSMENT OF THE SOIL ACIDITY PROBLEM IN ALBERTA AND NORTHEASTERN BRITISH COLUMBIA

1977 ◽  
Vol 57 (2) ◽  
pp. 157-164 ◽  
Author(s):  
D. C. PENNEY ◽  
M. NYBORG ◽  
P. B. HOYT ◽  
W. A. RICE ◽  
B. SIEMENS ◽  
...  
Keyword(s):  
British Columbia ◽  
Soil Ph ◽  
Soil Acidity ◽  
Field Experiments ◽  
Trifolium Pratense ◽  
Acid Soil ◽  
Acid Soils ◽  
Red Clover ◽  
Hordeum Vulgare L ◽  
Crop Species

The amount of cultivated acid soil in Alberta and northeastern British Columbia was estimated from pH values of farm samples analyzed by the Alberta Soil Testing Laboratory, and the effect of soil acidity on crops was assessed from field experiments on 28 typical acid soils. The field experiments consisted of two cultivars of barley (Hordeum vulgare L.) and one cultivar each of rapeseed (Brassica campestris L.), red clover (Trifolium pratense L.) and alfalfa (Medicago sativa L.) grown with and without lime for 2 yr. There are about 30,000 ha of soils with a pH of 5.0 or less where soil acidity seriously restricts yields of all four crop species. There are approximately 300,000 ha with a soil pH of 5.1–5.5 where liming will on the average increase yields of alfalfa by 100%, yields of barley by 10–15%, and yields of rapeseed and red clover by 5–10%. There are a further 1,600,000 ha where soil pH ranges from 5.6 to 6.0 and liming will increase yields of alfalfa by approximately 50% and yields of barley, rapeseed and red clover by at least 4–5%.

Acid tolerance in the Rhizobium meliloti - Medicago symbiosis

1986 ◽  
Vol 37 (1) ◽  
pp. 55 ◽  
Author(s):  
JG Howieson ◽  
MA Ewing
Keyword(s):  
Rhizobium Meliloti ◽  
Acid Soil ◽  
Acid Soils ◽  
Acid Tolerance ◽  
Loamy Sand ◽  
Alkaline Soils ◽  
Regenerative Growth ◽  
Commercial Inoculant ◽  
Acid Tolerant ◽  
Second Year

Several strains of Rhizobium meliloti that originated from acid soils in Sardinia, Italy, were markedly superior in colonizing a moderately acid loamy sand (pH 5.0 in 1:5 0.01 M CaCl2) than two Australian commercial inoculant strains (U45 and CC169), and a group of strains that originated from alkaline soils in Syria and Iraq. Six Medicago hosts also varied greatly in their ability to achieve nodulation in this soil. M. polymorpha and M. murex were far superior in this respect to M. littoralis, M. truncatula and M. tornata. The most acid-tolerant strains of R. meliloti, WSM419 and WSM413, were able to nodulate a high proportion of plants of M. polymorpha and M. murex sown in the second year between 11 and 20 cm from the point of introduction of the rhizobia into the soil the previous year. It is suggested that these more saprophytically competent isolates of R. meliloti, combined with the species of Medicago more able to nodulate readily in acid soil, will extend the range of soils suitable for successful regenerative growth of these species.

Calcium modifies pH effects on the growth of acid-tolerant and acid-sensitive Rhizobium meliloti

1992 ◽  
Vol 43 (3) ◽  
pp. 765 ◽  
Author(s):  
JG Howieson ◽  
AD Robson ◽  
LK Abbott
Keyword(s):  
Soil Acidity ◽  
Rhizobium Meliloti ◽  
Acid Soils ◽  
Defined Medium ◽  
Ph Effects ◽  
Poor Growth ◽  
Growth And Survival ◽  
Ph Values ◽  
Acid Tolerant ◽  
Selection Of

The growth of Rhizobium meliloti is sensitive to soil acidity, and its poor growth and survival limits the production from Medicago spp. on acid soils. In the selection of acid tolerant rhizobia for medics, growth in acidified laboratory media has been poorly related to persistence in acid soils. However, the Ca concentration in laboratory media may have been inadequate for growth of some rhizobial strains at low pH. Therefore, acid-tolerant and acid-sensitive strains of R. meliloti were grown in a buffered, defined medium at a range of Ca and P concentrations, and at several pH values. Growth rate was increased by increasing the Ca concentration from 200 to 2000 8M at low (5-70) and moderate (6.50) pH, but not at pH 7.30. Thus, the Ca requirement for the growth of R. meliloti under acid conditions is much higher than previously thought.

Strategies for control of Phoma black stem in annual Medicago species

1989 ◽  
Vol 29 (5) ◽  
pp. 635 ◽  
Author(s):  
MJ Barbetti
Keyword(s):  
Disease Severity ◽  
Medicago Truncatula ◽  
Field Trial ◽  
Subsequent Development ◽  
Field Site ◽  
Infected Seed ◽  
Annual Medicago ◽  
And Control ◽  
Medicago Species ◽  
Selection Of

Strategies for control of Phoma black stem disease in annual Medicago species through selection of cultivars with increased resistance to Phoma medicaginis, fungicidal spray applications to swards, and fungicidal control of seedborne infection, were investigated. Fiftyseven annual Medicago cultivars and lines were screened for resistance in the field in 1 m rows over 2 consecutive seasons. There were significant differences in resistance among species and also between lines and cultivars of any particular species. Three M. rugosa cultivars were very highly resistant and most cultivars and lines showed some resistance. In a field trial, the fungicides benomyl, carbendazim, flutriafol, propiconazole, thiabendazole and triadimefon were tested for their efficacy in controlling Phoma black stem disease. All fungicides reduced disease severity in the sward and, except for thiabendazole, the percentage burrs with Phoma lesions. The role and control of seed-borne P. medicaginis in causing Phoma black stem disease in Medicago truncatula and M. polymorpha var. brevispina at a field site was also investigated. Seed-borne P. medicaginis caused subsequent development of Phoma black stem disease in swards sown with infected seed. Disease appeared earlier, developed faster and became much more severe in M. truncatula cv. Cyprus than in M. polymorpha cv. Serena. Application of benomyl seed treatments (0.1 and 0.5% w/w) resulted in only a 4-5 week delay in the onset of Phoma black stem symptoms.

scholarly journals Fertility Status of Acid Soils under Different Land Use Types in Wolaita Zone, Southern Ethiopia

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Mesfin Kassa Cholbe ◽  
Fassil Kebede Yeme ◽  
Wassie Haile Woldeyohannes
Keyword(s):  
Land Use ◽  
Soil Acidity ◽  
Acid Soil ◽  
Acid Soils ◽  
Southern Ethiopia ◽  
Land Use Type ◽  
Grazing Land ◽  
Exchangeable Acidity ◽  
Fertility Status ◽  
Land Use Types

Information on soil fertility status of acid soil of a particular area as affected by land use type is important for developing sound soil management systems for improved and sustainable agricultural productivity. The main objective of this study was to assess the fertility status and effect of land use change on soil physicochemical properties. In this study, adjacent three land use types, namely, enset-coffee, crop, and grazing land use were considered in four districts (i.e., Bolos Sore, Damot Gale, Damot Sore, and Sodo Zuria) of Wolaita Zone, southern Ethiopia. Soil samples were collected from a depth of 0–20 cm from each land use type of the respective districts for physicochemical analyses. The results showed that land use types significantly affected ( P ≤ 0.05 ) soil properties such as bulk density, available P, exchangeable potassium, exchangeable acidity, exchangeable bases (Na, K, Ca, Mg), exchangeable acidity, and CEC. Besides, soil pH, OC, and TN were influenced significantly ( P ≤ 0.05 ) both by districts and land use types. The very strongly acidic soils were found predominantly in the crop and grazing lands whereas a neutral acidity level was found in the enset-coffee land use type of four districts. In conclusion, the study proves that land use type change within the same geographic setting can affect the severity of soil acidity due to over cultivation and rapid organic matter decomposition. Finally, the study recommends an in-depth study and analysis on the root causes in aggravating soil acidity under crop and grazing land use types.

A description of acid soils and the relationships between properties of acid soils and the nutrient status of grazed pastures in the southeast of South Australia

Soil Research ◽  
1989 ◽  
Vol 27 (1) ◽  
pp. 149 ◽  
Author(s):  
TJV Hodge ◽  
DC Lewis
Keyword(s):  
Soil Ph ◽  
Acid Soil ◽  
South Australia ◽  
Acid Soils ◽  
Subterranean Clover ◽  
Nutrient Status ◽  
Critical Value ◽  
Soil Types ◽  
Positive Correlations ◽  
Extractable Soil

Areas of low soil pH in the south-east of South Australia were delineated by using previously submitted soil samples and soil association maps. A survey was then undertaken in the major soil associations to determine the severity and characteristics of highly acid soils. The acid soil types identified were a siliceous sand over clay (Db/Dy) and a siliceous sand over organic matter/sesquioxide pan (Uc). The top 2.5 cm of both soil types was significantly less acid than the remaining portion of the A horizon, with pH decreasing rapidly with depth until the B horizon, where a substantial soil pH increase occurred. As soil pH (0.01 M CaCl2) decreased below 4.5, extractable soil aluminium (0.01 M CaCl2) increased rapidly, to a maximum extractable concentration of 17 �g g-l. These soil types were also found to be deficient in both phosphorus and potassium, with 65% of the sites having extractable phosphorus concentrations below the critical value of 20 �g g-1 and 35% below the critical value for extractable potassium of 80 �g g-l. For subterranean clover, significant positive correlations were observed between soil pH and plant calcium and sulfur, and between extractable soil aluminium and plant aluminium. Significant negative correlations were observed between soil pH and plant manganese and between extractable soil aluminium and plant calcium and magnesium. For ryegrass, significant positive correlations were observed between extractable soil aluminium and plant aluminium and manganese. Significant negative correlations were observed between soil pH and plant manganese and between extractable soil aluminium and plant calcium. No other significant correlations were obtained. The results are discussed in relation to further acidification and management of these acid siliceous sands.

Effects of calcium carbonate and ammonium sulphate on manganese toxicity in an acid soil

1971 ◽  
Vol 22 (2) ◽  
pp. 201 ◽  
Author(s):  
A Siman ◽  
FW Crodock ◽  
PJ Nicholls ◽  
HC Kirton
Keyword(s):  
Ammonium Sulphate ◽  
Soil Ph ◽  
Acid Soil ◽  
Manganese Content ◽  
Acid Soils ◽  
Manganese Toxicity ◽  
Water Soluble ◽  
North Coast ◽  
The North ◽  
Linear Relationships

The effects of increasing rates of lime and ammonium sulphate on French beans were studied on an acid red basaltic soil (pH 4.5-4.8), rich in manganese, on the north coast of New South Wales. Addition of lime resulted in an increased plant yield, a higher soil pH, and a marked reduction in available soil manganese and plant manganese. Applications of 2 or more tons lime per acre corrected manganese toxicity. Ammonium sulphate applications acidified the soil, increased manganese levels in both soil and plant tissue, and increased the frequency of manganese toxicity symptoms at less than 2 tons lime per acre. At pH 4.7-4.8, exchangeable and water-soluble manganese levels were sensitive to slight changes in reaction. Changes in pH between 5.2 and 6.0 caused only slight alterations in manganese levels in soil and plants. Two tons lime per acre reduced the level of manganese in the soil to about half that in the untreated soil, whereas 3 tons lime was necessary to halve the level of manganese in plants. Close linear relationships were found between rates of lime application and pH, between exchangeable and water-soluble manganese, and between both water-soluble and exchangeable soil manganese and plant manganese. Hyperbolic relationships were found between lime and manganese in soil and plants and also between pH and manganese fractions. Toxic levels of manganese in soil and leaves varied seasonally and yearly, and symptoms usually appeared when the manganese content of the first mature leaves was greater than 600 p.p.m. in the winter crop. Symptoms were more closely related to high levels of plant manganese than to soil manganese. The results of this trial indicate that soil and plant analyses are useful for predicting manganese toxicity in acid soils.

Yield responses to lime of wheat and barley on acid soils in north-eastern Victoria

1989 ◽  
Vol 29 (2) ◽  
pp. 209 ◽  
Author(s):  
DR Coventry ◽  
BR Walker ◽  
GR Morrison ◽  
MT Hyland ◽  
JC Avery ◽  
...  
Keyword(s):  
Soil Ph ◽  
Acid Soils ◽  
Lime Treatment ◽  
Crop Species ◽  
Yield Increase ◽  
Exchangeable Al ◽  
North Eastern ◽  
Acid Tolerant ◽  
Ph Range ◽  
Yield Responses

Liming experiments were conducted at 13 sites (soil pH range 4.99-6.27, 0-10 cm depth) in the dryland cropping region of north-eastern Victoria with wheat grown at all sites and barley at 3 sites. Lime increased wheat yields at 9 of the 13 sites with the acid sensitive cultivar Oxley, but the yield increase was not correlated (r2=0.07) with exchangeable Al. Exchangeable A1 was closely related to pH (in 0.01 mmol/L CaCl2). The acid-tolerant wheat cultivars (Matong and Millewa) out-yielded Oxley at a soil pH (CaCl2) of 4.7 and the acid-tolerant cultivars were less responsive to liming. The barley responded to the lime treatment at each of the 3 sites. The use of acid tolerant crop species is recommended on these soils, but an improvement in the predictability of a lime response is required before liming is widely recommended.

Sign in / Sign up

Export Citation Format

Share Document