Pathological changes associated with Oesophagostomum columbianum infestations in sheep: Serum protein changes after first infestation

1967 ◽  
Vol 18 (5) ◽  
pp. 821 ◽  
Author(s):  
C Dobson
Keyword(s):  
Cellulose Acetate ◽  
Serum Protein ◽  
Alimentary Tract ◽  
Serum Proteins ◽  
Immunological Response ◽  
Total Serum ◽  
Pathological Changes ◽  
Cellulose Acetate Electrophoresis ◽  
Globulin Level ◽  
Oesophagostomum Columbianum

The serum proteins of worm-free crossbred lambs were found to vary over a period of 10 weeks. Infestation of these lambs with 2000 infective Oesophagostomum columbianum larvae also caused marked changes in various serum proteins separated by cellulose acetate electrophoresis. The total serum proteins of infested sheep remained static or decreased slightly over the period of observation. There was a marked hypoalbuminaemia (measured in grams per cent.) in the infested lambs. The α-globulin retained the same level as in the controls; this was attributed to compensatory synthesis to overcome the osmotic changes produced by albumin loss. The ß1-globulin decreased throughout infestations; both albumin and ß1-globulin could have been lost through inflammation of the alimentary tract. The ß2-globulin increased very greatly during infestation; this was attributed to an immunological response. The y-globulin level at first decreased, but after 10 days the level increased at a rate commensurate with that in the control lambs. This increase was also attributed to an immunological response.

Estimation of Serum Proteins by Electrophoresis on Cellulose Acetate

1972 ◽  
Vol 18 (12) ◽  
pp. 1541-1542 ◽  
Author(s):  
J W Keyser ◽  
G L Watkins
Keyword(s):  
Cellulose Acetate ◽  
Serum Protein ◽  
Serum Proteins ◽  
Standard Procedure ◽  
Total Serum Protein ◽  
Total Serum ◽  
Cellulose Acetate Electrophoresis ◽  
Ponceau S ◽  
Reference Serum ◽  
Electrophoresis Of Proteins

Abstract We have evaluated results for albumin obtained by a standard procedure for cellulose acetate electrophoresis of proteins in serum. The Ponceau S-stained albumin and globulins were eluted and the albumin was calculated by the generally accepted formula [(albumin-bound dye absorbance/absorbance of total protein-bound dye) x total serum protein concn] and by the formula (absorbance of albumin-bound dye in test/absorbance of albumin-bound dye in a reference serum) x concn of albumin in reference serum. The ratio of values by the first and second methods ranged from 0.93 to 1.30, the first giving the higher results in cases of discrepancy. These findings confirm the limitations in accurately calculating any serum-protein fraction by the first method. The second method appears to be the more accurate.

Evaluation of a Cellulose-Acetate Electrophoresis System for Serum Protein Fractionation

1965 ◽  
Vol 11 (10) ◽  
pp. 937-942 ◽  
Author(s):  
Alex Kaplan ◽  
John Savory
Keyword(s):  
Cellulose Acetate ◽  
Serum Protein ◽  
Blood Donors ◽  
Serum Proteins ◽  
Normal Values ◽  
Protein Fractionation ◽  
Cellulose Acetate Electrophoresis ◽  
Cellulose Acetate Membranes ◽  
Control Serum ◽  
Electrophoresis System

Abstract A rapid system for the quantitative fractionation of serum proteins by electrophoresis on cellulose-acetate membranes was evaluated and found to be quite precise. The reproducibility (coefficient of variation) of the routine fractionation of a control serum carried out 40 times during a 15-week period was 2.4% for albumin and 14.2, 6.0, 6.1, and 5.2%, respectively, for the α1-, α2-, β-, and γ-globulin fractions. Normal values are given for serum protein fractions (specimens from nonprofessional blood donors) obtained by cellulose acetate electrophoresis.

Changes in Serum Protein During the Molt and Reproductive Cycles of the American Lobster (Homarus americanus)

1969 ◽  
Vol 26 (8) ◽  
pp. 2101-2109 ◽  
Author(s):  
John Barlow ◽  
George J. Ridgway
Keyword(s):  
Serum Protein ◽  
Serum Proteins ◽  
Homarus Americanus ◽  
Total Serum Protein ◽  
Total Serum ◽  
Molt Cycle ◽  
Serum Samples ◽  
Cellulose Acetate Electrophoresis ◽  
Quantitative Changes ◽  
Reproductive Cycles

The variations in the serum proteins of lobsters were studied by quantitative protein determinations, starch gel and cellulose acetate electrophoresis, and immunodiffusion. A series of bleedings from 15 lobsters provided serum samples for each stage of the molt cycle. Total serum protein was highest before the molt and lowest directly after the molt. The total serum protein averaged 2.5 g/100 ml in the intermolt lobsters and variation among individuals was limited. Variation of serum proteins in an individual during the molt cycle included changes in the electrophoretic and immunodiffusion pattern. Quantitative changes during the cycle indicate a relative shift in the concentrations of various proteins. One component of serum protein was associated only with the development of eggs and another component was present only during premolt and directly after molt.

scholarly journals Canine Serum Protein Responses to X-Irradiation

1984 ◽  
Vol 3 (4) ◽  
pp. 317-321
Author(s):  
H. J. Esber ◽  
P. Zavorskas ◽  
H. Rosenkrantz
Keyword(s):  
Lymph Nodes ◽  
Cellulose Acetate ◽  
Radiation Damage ◽  
Serum Protein ◽  
Plasma Protein ◽  
Immunoglobulin G ◽  
Inflammatory Process ◽  
Serum Proteins ◽  
Cellulose Acetate Electrophoresis ◽  
X Irradiation

It was of interest, in the absence of such data, to investigate the effects of x-irradiation on individual serum proteins, including immunoglobulin G. Doses of 600 and 800 rads to the abdominal areas of immature beagles were sufficient to evoke emesis and hemorrhages of lymph nodes and perilobular areas of the liver. Seven days after irradiation of 8–12 male dogs/dose, serum specimens were analyzed by cellulose acetate electrophoresis and radial immunodiffusion. Radiation reduced serum protein and albumin by 12%, β2 by 27%, γ-globulin by 19%, and IgG by 35%, while increasing α2 by 50%. These results are in conformity with the radiation damage to lymph nodes (IgG decrease) and liver (plasma protein fall) and initiation of the inflammatory process (α2 rise).

Pathological changes associated with Oesophagostomum columbianum infestations in sheep: Serum protein changes in relation to multiple infestation, intensity of infestation, and immunity

1967 ◽  
Vol 18 (6) ◽  
pp. 931 ◽  
Author(s):  
C Dobson
Keyword(s):  
Infected Animal ◽  
Complement Fixation ◽  
Serum Proteins ◽  
Immunological Response ◽  
Total Serum ◽  
Infective Larvae ◽  
Infestation Intensity ◽  
Oesophagostomum Columbianum ◽  
Antibody Titres ◽  
Osmotic Balance

A relationship between size of infesting dose and multiple infestation and the total serum proteins was demonstrated. During primary infestations with low and medium-sized doses of infective larvae, the total serum proteins fell. After infestation with large larval doses or after multiple infestations the total serum proteins increased. Hypoalbuminaemia could be demonstrated after both primary and secondary infestations; also the animals given secondary infestation had failed to regain the level of serum albumin present before primary infestation. Both α- and ß1-globulins of sheep with either first or second infestation retained a level commensurate with, or decreased compared with, that in the control animals. These changes are discussed in relation to osmotic balance of the infected animal. The ß2-globulins increased during both primary and secondary infestations: however, the increase was greatest during the primary infestation. Increases in the y-globulin fraction also occurred but here the greatest increase occurred after secondary infestation. After primary infestation the y-globulin levels first fell and then increased as the infestation progressed. There was a correlation between the complement fixation, but not the passive haemagglutination, antibody titres and the increased serum ß2- and Y-globulin levels. However, the increased y-globulin levels after secondary infestation were too great to be solely accounted for by an immunological response.

Muscle Weight Relationship to Serum Proteins, Hemocytes, and Hepatopancreas in the Lobster, Homarus americanus

1967 ◽  
Vol 24 (11) ◽  
pp. 2339-2354 ◽  
Author(s):  
James E. Stewart ◽  
John W. Cornick ◽  
Diane M. Foley ◽  
M. F. Li ◽  
C. M. Bishop
Keyword(s):  
Serum Protein ◽  
Physiological Condition ◽  
Serum Proteins ◽  
Homarus Americanus ◽  
Total Serum Protein ◽  
Total Serum ◽  
Experimental Conditions ◽  
Muscle Weight ◽  
Beef Liver ◽  
The Relationship

Total serum protein values, hemocyte numbers, and muscle weights were determined for 216 intermolt lobsters immediately after their capture, and for 230 others held captive under a variety of dietary and environmental conditions. Average muscle values ranged from approximately 13% to the more normal 20–25% of the live animals' weight, depending upon experimental conditions. The total serum protein up to a level of 55 mg/ml was shown to be a reliable indicator of muscle weights, although the relationship was not identical for all lobster groups. It appeared to be modified chiefly by the areas from which the different groups were taken. Diet was more important than the temperatures (5 to 14 C) in affecting changes in muscle and serum protein values. Starvation caused a greater reduction (50 to 70%) in the size of the hepatopancreas than in the muscle. Histological examination of the hepatopancreatic tissue showed that the lipid content was markedly reduced upon starvation and that a degeneration of this organ was apparent for lobsters fed a beef liver and herring diet. Measurement of serum proteins would appear to be a useful technique in experiments on lobster nutrition and have value, within specified limits, for assessing the physiological condition of wild lobsters.

scholarly journals Serum proteinogram of the Campeiro horse

2019 ◽  
Vol 71 (2) ◽  
pp. 363-368
Author(s):  
A.F. Souza ◽  
J. Schade ◽  
A.F. Ramos ◽  
M.S.M. Albuquerque ◽  
G.V. Fonteque ◽  
...  
Keyword(s):  
Serum Protein ◽  
Serum Proteins ◽  
Reference Interval ◽  
Total Serum Protein ◽  
Total Serum ◽  
Serum Concentrations ◽  
Reproductive Condition ◽  
Serum Fractions ◽  
Pregnancy Status ◽  
Biuret Method

ABSTRACT The aim of this study to measure the fractions of the total serum proteins of the Campeiro horse and identify the influences of biological variants. Blood samples were taken in 138 horses of the breed Campeiro for measuring the concentration of total serum protein by the biuret method. Serum concentrations of protein fractions were measured by electrophoresis using agarose gel. Groups were formed according to age, sex and reproductive condition. The average values of serum fractions: albumin (2.85±0.36g/dl), alpha 1 (0.28±0.11g/dl), alpha 2 (0.26±0.08g/dL) beta 1 (0.57±0.15g/dl), beta 2 (0.89±0.28g/dL), gamaglobulinas (1.86±0.34g/dL), albumin/globulin ratio (0.75±0.18) and 2.5% percentile and 97.5% had slight differences in relation to the reference interval proposed for the species. They observed higher values of alpha 1 and 2 globulins in the group from that had six to eight years old and gammaglobulins in group above 13 years old. Serum protein concentrations were similar in horses and mares and between non-pregnant and pregnant. Sex and pregnancy status did not affect serum proteinogram. Alpha and gammaglobulins have higher values as the age increases. Serum proteinogram of Campeiro horses shows variations that have to be considered in the interpretation of laboratory tests.

Precipitation of serum proteins by the lectin from wheat-germ.

1987 ◽  
Vol 33 (1) ◽  
pp. 185-186 ◽  
Author(s):  
W E Schreiber ◽  
L Whitta
Keyword(s):  
Alkaline Phosphatase ◽  
Serum Protein ◽  
Wheat Germ ◽  
Serum Proteins ◽  
Total Serum Protein ◽  
Total Serum ◽  
Wheat Germ Lectin

Abstract We investigated the composition of the precipitate that forms when wheat-germ lectin derived from Triticum vulgaris is added to serum. A number of serum proteins are precipitated, representing about 2.5% of the total serum protein. This study demonstrates that the interaction of this lectin with the bone isoenzyme of alkaline phosphatase is not specific.

Imprecision of quantification of serum protein fractions by electrophoresis on cellulose acetate.

1986 ◽  
Vol 32 (2) ◽  
pp. 356-357 ◽  
Author(s):  
S N Kahn ◽  
L P Strony
Keyword(s):  
High Resolution ◽  
Serum Albumin ◽  
Cellulose Acetate ◽  
Serum Protein ◽  
Protein Fractions ◽  
Visual Interpretation ◽  
Cellulose Acetate Electrophoresis ◽  
Trained Observer ◽  
Electrophoretic Patterns

Abstract We studied the precision of densitometric quantification of the protein zones resolved by cellulose acetate electrophoresis. Replicate analyses of patients' samples by a single technologist showed mean CVs ranging from 2.9% for serum albumin to 9.5% for alpha 1-globulin. There were marked differences in measurements obtained by replicate analysis of the same samples by two experienced technologists. We calculated what changes in fractional concentrations would be analytically significant and concluded that densitometry of cellulose acetate electrophoretograms can only be semi-quantitative. We suggest that visual interpretation of high-resolution electrophoretic patterns by a trained observer can replace densitometry in most cases.

Sign in / Sign up

Export Citation Format

Share Document