Determination of total body water of rabbits exposed to high external environmental temperatures using a tritium labeling method

1969 ◽  
Vol 68 (2) ◽  
pp. 870-872
Author(s):  
M. R. Zel'tser
Keyword(s):  
Total Body Water ◽  
Body Water ◽  
Total Body ◽  
Labeling Method ◽  
Environmental Temperatures ◽  
Tritium Labeling

Determination of total body water in uraemic patients by bioelectrical impedance

1993 ◽  
Vol 8 (8) ◽  
pp. 716-719 ◽  
Author(s):  
C. H. Kong ◽  
C. M. Thompson ◽  
C. A. Lewis ◽  
P. D. Hill ◽  
F. D. Thompson
Keyword(s):  
Total Body Water ◽  
Bioelectrical Impedance ◽  
Body Water ◽  
Total Body

scholarly journals Determination of total body water by a simple and rapid mass spectrometric method

1996 ◽  
Vol 31 (1) ◽  
pp. 108-111 ◽  
Author(s):  
B. K. Van Kreel ◽  
F. Van der Vegt ◽  
M. Meers ◽  
T. Wagenmakers ◽  
K. Westerterp ◽  
...  
Keyword(s):  
Total Body Water ◽  
Mass Spectrometric ◽  
Body Water ◽  
Spectrometric Method ◽  
Mass Spectrometric Method ◽  
Total Body ◽  
Rapid Mass

scholarly journals Total body water and extracellular water measurements throughin vivodilution of D2O and bromide as tracers

2003 ◽  
Vol 17 (2-3) ◽  
pp. 603-611 ◽  
Author(s):  
Giuseppe Sergi ◽  
Roberta Bertani ◽  
Irene Calliari ◽  
Lucia Lupoli ◽  
Adriano Berton ◽  
...  
Keyword(s):  
Total Body Water ◽  
Body Fluids ◽  
Body Water ◽  
Energy Dispersive ◽  
Extracellular Water ◽  
X Ray ◽  
Pathological Conditions ◽  
Ft Ir ◽  
Total Body

Large interest is into the determination of body fluids because of changes in body water in a wide variety of physiological and pathological conditions. Here methods based on dilution of tracers, are reported, for the extracellular water (ECW) and total body water (TBW) determinations on the same sample. As for ECW, bromide is used as tracer and its concentration is determined by energy dispersive X-ray spectrometry; while as for TBW, D2O is used as tracer and HOD is determined by FT IR. Both methods represent significant improvements with respect the procedures described in the literature in terms of availability, reproducibility and accuracy.

The effect of route of dosing and method of estimation of tritiated water space on the determination of total body water and the prediction of body fat in sheep

1974 ◽  
Vol 82 (1) ◽  
pp. 105-112 ◽  
Author(s):  
B. S. W. Smith ◽  
A. R. Sykes
Keyword(s):  
Body Weight ◽  
Body Fat ◽  
Total Body Water ◽  
Specific Activity ◽  
Tritiated Water ◽  
Multiple Correlation Coefficient ◽  
Body Water ◽  
Total Body ◽  
Water Space

SUMMARYEight mature female sheep were offered a ration which maintained body weight constant during a 20-week period. During the final 10 weeks a comparison was made in each animal of the pattern of equilibration and urinary losses of tritiated water during 8 h after dosing by four different routes. These were intravenous, intraperitoneal, intraruminal and a combination of the intraperitoneal and intraruminal routes. Tritiated water spaces were calculated from (a) the 8-h plasma specific activity and (b) by extrapolation to zero time of the plasma specific activities during the 7 days after injection. At the end of the experiment the fat and water contents of the bodies of the sheep were determined directly.Complete equilibration of tritiated water between plasma and rumen water was not achieved in all animals 8 h after intravenous or intraperitoneal injection but was when the rumen was primed by the combination of intraperitoneal and intraruminal dosing. After intraruminal dosing equilibration was not achieved in any animal within 8 h of dosing.Urinary losses of marker were lower after intraruminal dosing but otherwise averaged 4–5 % of the dose/1 urine. This was equivalent to 0·3–6·7% of the dose for individual sheep.Errors resulting from incomplete equilibration and urinary loss of marker did not influence the efficiency of prediction of total body water from tritiated water space. The multiple correlation coefficient relating body fat with empty body weight and its water content was very high (r = 0·99). Errors introduced into this relationship by the inclusion of gut water in the prediction equations were apparently of a similar magnitude to those resulting from the errors in the estimation of tritiated water space.The extrapolation method for the determination of tritiated water space was shown to have the same accuracy as equilibration techniques under these controlled dietary conditions.

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