Validation of an Alt locus for aluminium tolerance scored with eriochrome cyanine R staining method in barley cultivar Honen (Hordeum vulgare L.)

2006 ◽  
Vol 57 (1) ◽  
pp. 113 ◽  
Author(s):  
Junping Wang ◽  
Harsh Raman ◽  
Barbara Read ◽  
Meixue Zhou ◽  
Neville Mendham ◽  
...  
Keyword(s):  
Hordeum Vulgare ◽  
Ssr Markers ◽  
Acid Soils ◽  
Aluminium Tolerance ◽  
Al Tolerance ◽  
Hordeum Vulgare L ◽  
Chromosome 4H ◽  
Barley Germplasm ◽  
Al Phytotoxicity ◽  
Single Sequence

Aluminium (Al) phytotoxicity is one of the major constraints for barley (Hordeum vulgare L.) production on acid soils. Genetic variation for Al tolerance is present in barley germplasm. Previous studies have shown that Al tolerance in cv. Dayton and WB229 is controlled by a single locus on 4H. The present study was conducted to investigate the genetic control of Al tolerance in Japanese barley cv. Honen, and validate the linkage relationship between the Al tolerance locus and the molecular markers established previously. An F3 progeny representing 94 F2s from the cross of F6ant28B48-16 (Al sensitive) × Honen (Al tolerant) was evaluated for Al tolerance by staining roots with eriochrome cyanine R. A single Al tolerance locus Alt was validated on the long arm of chromosome 4H in Honen. Single sequence repeat (SSR) markers Bmag353 and HVM68 flanked the Alt locus at a distance of 1.6 ± 0.9 and 5.1 ± 1.7 cM, respectively. Our results have shown that the linkage between SSR markers and the Al tolerance locus can be used to characterise the ‘unknown source’ for Al tolerance.

scholarly journals Aluminium tolerance in barley (Hordeum vulgare L.): Physiological mechanisms, genetics and screening methods

2006 ◽  
Vol 7 (10) ◽  
pp. 769-787 ◽  
Author(s):  
Jun-ping Wang ◽  
Harsh Raman ◽  
Guo-ping Zhang ◽  
Neville Mendham ◽  
Mei-xue Zhou
Keyword(s):  
Hordeum Vulgare ◽  
Aluminium Tolerance ◽  
Screening Methods ◽  
Hordeum Vulgare L ◽  
Physiological Mechanisms

EST-SSR markers derived from an elite barley cultivar (Hordeum vulgare L. ‘Morex’): polymorphism and genetic marker potential

Genome ◽  
2009 ◽  
Vol 52 (8) ◽  
pp. 665-676 ◽  
Author(s):  
Livinus C. Emebiri
Keyword(s):  
Hordeum Vulgare ◽  
Ssr Markers ◽  
Cellulose Synthase ◽  
Cdna Libraries ◽  
Functional Markers ◽  
Hordeum Vulgare L ◽  
Glucanase Activity ◽  
Phenotypic Data ◽  
Trait Locus ◽  
High Level

Microsatellites or simple sequence repeats have become the markers of choice for marker-assisted selection because of their low template DNA requirement, high reproducibility, and high level of polymorphism. This study investigated a new set of barley ( Hordeum vulgare L.) EST-derived SSR markers designed to target gene sequences expressed during grain development, as they are more likely to be important in determining grain quality. The EST sequences (HVSMEh and HVSMEi) were derived from cDNA libraries of the elite six-rowed cultivar Morex, made from spikes harvested at 5 to 45 days after pollination. Approximately half of the 110 SSR markers derived from the ESTs were polymorphic in a panel of 8 diverse barley genotypes, with PIC values between 0.19 and 0.79. Twenty of the new markers were mapped to chromosomal locations using 2 doubled haploid populations. To demonstrate marker potential, quantitative trait locus (QTL) analyses were carried out with phenotypic data on wort β-glucan content and β-glucanase activity, two traits with a long history of genetic studies. Most of the EST-SSR markers mapped to within 10 cM of the cellulose synthase (HvCesA) and cellulose synthase-like (HvCslF) genes, which provides highly informative functional markers for tracking these genes in breeding programs. It was also observed that on any given chromosome, the QTL for β-glucan content and β-glucanase activity were rarely coincident but tended to occur in adjacent intervals along chromosomal regions, which agreed with their independent genetic basis; the adjacent localization may be important for coordination of cell wall degradation during germination and malting.

A study of genetic diversity of colored barley (Hordeum vulgare L.) using SSR markers

2014 ◽  
Vol 62 (3) ◽  
pp. 395-406 ◽  
Author(s):  
Wei Hua ◽  
Xiaoqin Zhang ◽  
Jinghuan Zhu ◽  
Yi Shang ◽  
Junmei Wang ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Hordeum Vulgare ◽  
Ssr Markers ◽  
Hordeum Vulgare L

Development of gene-specific markers for acid soil/aluminium tolerance in barley (Hordeum vulgare L.)

2013 ◽  
Vol 32 (1) ◽  
pp. 155-164 ◽  
Author(s):  
Miao Bian ◽  
Irene Waters ◽  
Sue Broughton ◽  
Xiao-Qi Zhang ◽  
Meixue Zhou ◽  
...  
Keyword(s):  
Hordeum Vulgare ◽  
Acid Soil ◽  
Aluminium Tolerance ◽  
Hordeum Vulgare L

Genetic diversity, population structure and relationship of Ethiopian barley (Hordeum vulgare L.) landraces as revealed by SSR markers

2022 ◽  
Vol 101 (1) ◽  
Author(s):  
Allo A. Dido ◽  
M. S. R. Krishna ◽  
Ermias Assefa ◽  
Dawit T. Degefu ◽  
B. J. K. Singh ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Hordeum Vulgare ◽  
Ssr Markers ◽  
Hordeum Vulgare L ◽  
Relationship Of

Recent molecular breeding advances for improving aluminium tolerance in maize and sorghum.

2021 ◽  
pp. 318-324
Author(s):  
Claudia Teixeira Guimaraes ◽  
Jurandir Vieira de Magalhaes
Keyword(s):  
Target Genes ◽  
Acid Soils ◽  
Genetic Effect ◽  
Al Toxicity ◽  
Aluminium Tolerance ◽  
Al Tolerance ◽  
Toxic Compound ◽  
The World ◽  
Breeding Programmes ◽  
Wide Adaptation

Abstract Citrate transporters belonging to the multidrug and toxic compound extrusion (MATE) family of membrane transporters in sorghum and maize, SbMATE and ZmMATE1, respectively, play a major role in aluminium (Al) tolerance. However, these MATE members show regulatory differences, as well as peculiarities in their genetic effect and mode of action. These aspects, which are discussed in this chapter, have to be considered to design successful breeding programmes in order to achieve maximum Al tolerance and, consequently, to improve grain and biomass production in regions of the world with Al toxicity. As shown in this chapter, target genes with major effects and molecular tools are available for marker-assisted breeding for improving Al tolerance both in sorghum and maize. However, wide adaptation to acid soils should be sought by pyramiding genes controlling different traits such as drought tolerance, P acquisition, resistance to diseases and other stresses commonly found in each agroecological environment.

Novel barley (Hordeum vulgare L.) germplasm resistant to acidic soil

2010 ◽  
Vol 61 (7) ◽  
pp. 540 ◽  
Author(s):  
J. S. Moroni ◽  
K. Sato ◽  
B. J. Scott ◽  
M. Conyers ◽  
B. J. Read ◽  
...  
Keyword(s):  
Hordeum Vulgare ◽  
Acid Soil ◽  
Solution Culture ◽  
Acidic Soil ◽  
Acidic Soils ◽  
Hordeum Vulgare L ◽  
Wheat Genotypes ◽  
Australian Standard ◽  
Barley Germplasm ◽  
Barley Genotypes

Improving the resistance of barley (Hordeum vulgare L.) to acidic soils is an important goal of several barley breeding programs around the world. The identification and utilisation of novel barley sources resistant to aluminium (Al) may provide a significant and rapid advance towards that goal. Barley standards and screening protocols for selecting barley germplasm resistant to Al in nutrient solution and acidic soil were reevaluated. The assays used were quantitative in nature and were suitable for genotypic- and seedling-based selections. Although there was a broad agreement between the solution culture assays and soil assays in the ranking of genotypes it obscured the fact that misclassification of genotypes is common. Brindabella was shown to be better suited than Dayton (the current barley standard resistant to Al) as the Australian standard for resistance to acidic soils. A seedling-based Al pulse-recovery assay and an acidic soil assay were used to characterise 41 genotypes from the South and East Asian Barley Core Collection (SEA-BCC). In addition, in the acidic soil assays several standard barley and wheat genotypes were included. Three SEA-BCC genotypes were more resistant than Dayton to acidic soil while several others were similar to Dayton. The most resistant SEA-BCC genotypes Honen, Ohichi and Zairai Tanbo were of Japanese origin. Misclassification of barley genotypes and wheat genotypes for resistance to soil acidity between solution culture and acid soil assay provided strong evidence for the unsuitability of solution culture assay. Although in solution culture several barley genotypes were sensitive relative to wheat, in acidic soil they were not different from wheat. While the quest for resistant barley to acidic soils similar or better than resistant wheat still continues, it may be an unnecessary endeavour.

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