Genetic analysis of embryo, cytoplasmic, and maternal effects and their environment interactions for protein content in Brassica napus L.

2005 ◽  
Vol 56 (1) ◽  
pp. 69 ◽  
Author(s):  
J. G. Wu ◽  
C. H. Shi ◽  
H. Z. Zhang
Keyword(s):  
Brassica Napus ◽  
Protein Content ◽  
Genetic Model ◽  
Interaction Effects ◽  
Genetic Effects ◽  
Ge Interaction ◽  
Brassica Napus L ◽  
Diallel Mating Design ◽  
Selection For ◽  
Main Effects

A genetic model for quantitative traits of seeds in diploid plants was applied to estimate the genetic main effects and genotype × environment (GE) interaction effects for protein content (PC) of rapeseed (Brassica napus L.) by using 2 years of experimental data with a diallel mating design of 8 parents. Results showed that PC of rapeseed was simultaneously controlled by genetic effects of embryo, cytoplasm, and maternal plant, of which the maternal genetic effects were most important, followed by embryo and cytoplasmic genetic effects. Therefore, improvement of PC of rapeseed would be more efficient when selection is based on maternal plants than that on single seeds. Since the GE interaction effects accounted for about 60.10% of total genetic effect, they were more important than the genetic main effects, and selection for PC might be influenced by environmental conditions. The total narrow-sense heritability for PC was 64.17%, of which the interaction heritability was larger than the general heritability. Selection for improving PC can therefore be conducted in early generations. Maternal heritability (41.59%) was most important for PC, followed by cytoplasmic heritability (17.62%) and then by embryo heritability (5.25%). Based on prediction of genetic effects, parent Youcai 601 was better than others for increasing PC in rapeseed breeding.

scholarly journals Dynamic genetic effects on threonine content in rapeseed (Brassica napus L.) meal at different developmental stages

2011 ◽  
Vol 47 (No. 3) ◽  
pp. 101-113 ◽  
Author(s):  
G. Chen ◽  
J. Wu ◽  
Ch. Shi
Keyword(s):  
Brassica Napus ◽  
Developmental Stages ◽  
Rapeseed Meal ◽  
Interaction Effects ◽  
Genetic Effects ◽  
Narrow Sense ◽  
Ge Interaction ◽  
Brassica Napus L ◽  
Expression Of Genes ◽  
Main Effects

 Dynamic genetic effects on threonine content (TC) of rapeseed (Brassica napus L.) meal were analysed at 5 developmental times/stages using the genetic models for diploid plant seeds. Results indicated that the expression of diploid embryo, cytoplasmic and diploid maternal plant genes were all important for the performance of TC at various developmental times/stages of rapeseed, especially at the early and middle developmental stages. Among different genetic systems, TC was mainly controlled by the cumulative or net maternal main effects and the genotype × environment (GE) interaction effects, followed by the embryo main effects and GE interaction effects. The expression of genes was more easily influenced by the environmental factors at the first three developmental stages. The total narrow-sense heritabilities for TC on 15, 22, 29, 36 and 43 days post anthesis were 46.50, 62.60, 57.10, 84.70 and 59.50%, respectively, of which the interaction heritabilities were more important at the first three developmental stages of rapeseed. The improvement in TC of rapeseed meal could be expected by selection based on the higher narrow-sense heritabilities near maturity. The predicted genetic effects of parents showed that TC of progeny could be improved by using the parent Gaoyou 605.  

Association of a RAPD marker with linolenic acid concentration in the seed oil of rapeseed (Brassica napus L.)

Genome ◽  
1995 ◽  
Vol 38 (2) ◽  
pp. 414-416 ◽  
Author(s):  
P. K. Tanhuanpää ◽  
J. P. Vilkki ◽  
H. J. Vilkki
Keyword(s):  
Brassica Napus ◽  
Linolenic Acid ◽  
Acid Concentration ◽  
Seed Oil ◽  
Rapd Marker ◽  
Brassica Napus L ◽  
Breeding Programs ◽  
Selection For ◽  
Rflp Rapd ◽  
Low Linolenic

The F2 progeny (64 individuals) from the cross between oilseed rape (Brassica napus L.) cultivar Topas and R4 (a low linolenic mutation line) was analyzed with 8 RFLPs and 34 RAPDs to discover a genetic tag for gene(s) affecting linolenic acid concentration. According to variance analysis (ANOVA), one RAPD marker (25a) was significantly associated with linolenic acid content; the linolenic acid concentration in the seeds of F2 individuals showing the marker (includes both homo- and hetero-zygotes) was 7.43 ± 1.35% and in those lacking the marker was 5.70 ± 1.52%. Marker 25a may be used to facilitate selection for fatty acid composition in future breeding programs of oilseed rape.Key words: Brassica napus, RFLP, RAPD, linolenic acid.

Species and varietal differences in the proteins of rapeseed

1969 ◽  
Vol 47 (5) ◽  
pp. 679-685 ◽  
Author(s):  
A. J. Finlayson ◽  
R. S. Bhatty ◽  
C. M. Christ
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Brassica Napus ◽  
Amino Acid Composition ◽  
Protein Content ◽  
Acid Composition ◽  
Brassica Campestris ◽  
Sulfur Deficiency ◽  
Brassica Napus L ◽  
Sulfur Containing

Two proteins, previously described by the authors as BI (S020, buffer 12 S) and AIVS (S020, w 1.7 S) have been isolated from eight varieties of rapeseed belonging to Brassica campestris L. and Brassica napus L. species. These proteins have similar chromatographic and electrophoretic characteristics but differ in amino acid composition, particularly with regard to the sulfur-containing amino acids. One of the rapeseed samples was obtained from plants grown on sulfur-deficient soil. The sulfur deficiency produced, in the mature seed, a much reduced protein content and appeared to affect the structure of the protein BI.

Selection for fast germination in rapeseed (Brassica napus L. and B. Campestris L.)

Euphytica ◽  
1986 ◽  
Vol 35 (3) ◽  
pp. 835-842 ◽  
Author(s):  
Jane R. King ◽  
Z. P. Kondra ◽  
M. R. Thiagarajah
Keyword(s):  
Brassica Napus ◽  
Brassica Napus L ◽  
Selection For

COMPOSITIONAL CHANGES IN DEVELOPING PODS AND SEEDS OF OILSEED RAPE (BRASSICA NAPUS L.) AS AFFECTED BY POD POSITION ON THE PLANT

1979 ◽  
Vol 59 (3) ◽  
pp. 819-830 ◽  
Author(s):  
W. DIEPENBROCK ◽  
G. GEISLER
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Brassica Napus ◽  
Protein Content ◽  
Acid Composition ◽  
Dry Matter ◽  
Oil Content ◽  
Second Phase ◽  
Dry Matter Accumulation ◽  
Brassica Napus L

Plants of rape (Brassica napus L. ’Erglu’) were grown under controlled greenhouse conditions. The growth and composition of pods (hulls) and seeds was followed from the day when flowering took place to maturity. Pod development could be divided into three stages. In the first stage hull growth was more rapid than seed growth. Nitrogen accumulation in hulls occurred at a maximum and seeds were characterized by a high protein content but a low oil content and a fatty acid composition similar to that of photosynthetic tissue. During the second phase dry matter of hulls began to decrease, nitrogen was exported and the rates of seed dry matter accumulation grew to a maximum. At the same time the protein content of seeds declined while oil content increased rapidly. The fatty acid composition had reached the final condition. In the third stage hulls continued to lose dry matter and the rates of seed dry matter accumulation decreased dramatically. Pod growth was affected by the location of the pod on the plant. The individual seed weight and the fatty acid compvsition at 16 days after flowering indicated an accelerated development of seeds from axillary branches. Pods from the main branch generally produced the largest amount of seed dry matter. From these results the ideal high-yielding rape plant was characterized.

scholarly journals Estimation of Genetic Effects for Different Traits in Rapeseed (Brassica napus L.) using Line × Tester Crosses under Water-Stressed and Well-Watered Conditions

2019 ◽  
Vol 11 (29) ◽  
pp. 17-28
Author(s):  
Mehdi Jamshidmoghaddam ◽  
Ezatollah Farshadfar ◽  
Abdollah Najaphy ◽  
◽  
◽  
...  
Keyword(s):  
Brassica Napus ◽  
Genetic Effects ◽  
Brassica Napus L

scholarly journals Protein pattern of canola (Brassica napus L.) changes in response to salt and salicylic acid in vitro

2015 ◽  
Vol 52 (1-2) ◽  
pp. 19-36 ◽  
Author(s):  
Roya Razavizadeh
Keyword(s):  
Salicylic Acid ◽  
Brassica Napus ◽  
Protein Content ◽  
Soluble Protein ◽  
Interactive Effects ◽  
Total Soluble Protein ◽  
Protein Patterns ◽  
Soluble Protein Content ◽  
Brassica Napus L ◽  
Total Soluble Protein Content

Abstract The effect of salicylic acid (SA) on the salt (NaCl) tolerance mechanism was studied in canola plants (oilseed rape, Brassica napus L.) by molecular and physiological experiments in plant tissue culture. Seeds of B. napus ‘Ocapy’ were germinated at 0, 50, and 100 mM NaCl on Murashige and Skoog (MS) medium containing different levels (0, 2, and 5 μM) of SA for 4 weeks. Total chlorophyll, carotenoid, and flavonoid content increased in response to interactive effects of SA and NaCl treatments at some concentrations. Proline content was increased under salt and SA treatments in shoot and root tissues. Salt alone and in combination with SA increased the total soluble protein content of shoots only, while the different concentrations of SA in the culture media affected variously the total soluble protein content. Protein patterns of shoots and roots showed some remarkable differences, based on gel electrophoresis and the consequent analysis of bands by ImageJ program. The relative expression of 15 and 12 protein bands in shoots and roots, respectively, differed under the applied treatments. In addition, the protein profile indicated that salinity and SA regulate the expression of salt-stress-inducible proteins as well as induced de novo synthesis of specific polypeptides. The findings may help to explain the salt tolerance mechanisms and to produce salt-tolerant canola plants.

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