PCR-based molecular marker for the Bdv2 Thinopyrum intermedium source of barley yellow dwarf virus resistance in wheat

2001 ◽  
Vol 52 (12) ◽  
pp. 1383 ◽  
Author(s):  
P. Stoutjesdijk ◽  
S. J. Kammholz ◽  
S. Kleven ◽  
S. Matsay ◽  
P. M. Banks ◽  
...  
Keyword(s):  
Molecular Marker ◽  
Barley Yellow Dwarf Virus ◽  
Wheat Breeding ◽  
Addition Line ◽  
Thinopyrum Intermedium ◽  
Barley Yellow Dwarf ◽  
Pcr Product ◽  
Partial Amphiploid ◽  
Yellow Dwarf Virus ◽  
Pcr Based Molecular Marker

Because of the importance of BYDV in wheat production worldwide, and given the difficulties of bioassaying for resistance, a molecular marker was developed for the resistance known as Bdv2 that originates on the long arm of chromosome 7Ai1 of Thinopyrum intermedium. This resistance was identified in a partial amphiploid line TAF46, a disomic addition line to wheat (L1), a telosomic addition line (7Ai1 L), and a series of recombinants and translocations. A RAPD (random amplified polymeric DNA) marker for the resistant germplasm was cloned and sequenced, and primers were designed against that sequence to produce a sequence characterised amplified region (SCAR) marker. A single PCR product is produced only with genotypes carrying the resistance from any of the available recombinants. The cloned sequence, recommended primers, and PCR protocols are described. The usefulness of the marker has been demonstrated for following Bdv2 in segregating wheat breeding germplasm, with the imminent release of a BYDV-resistant cultivar.

Screening and analysis of differentially expressed genes from an alien addition line of wheat Thinopyrum intermedium induced by barley yellow dwarf virus infection

Genome ◽  
2004 ◽  
Vol 47 (6) ◽  
pp. 1114-1121 ◽  
Author(s):  
Shu-Mei Jiang ◽  
Long Zhang ◽  
Jun Hu ◽  
Rui Shi ◽  
Guang-He Zhou ◽  
...  
Keyword(s):  
Differentially Expressed Genes ◽  
Expressed Sequence Tag ◽  
Barley Yellow Dwarf Virus ◽  
Differentially Expressed ◽  
Addition Line ◽  
Alien Chromosome ◽  
Thinopyrum Intermedium ◽  
Barley Yellow Dwarf ◽  
Yellow Dwarf Virus ◽  
Expressed Sequence

The alien addition line TAI-27 contains a pair of chromosomes of Thinopyrum intermedium that carry resistance against barley yellow dwarf virus (BYDV). A subtractive library was constructed using the leaves of TAI-27, which were infected by Schizaphis graminum carrying the GAV strain of BYDV, and the control at the three-leaf stage. Nine differentially expressed genes were identified from 100 randomly picked clones and sequenced. Two of the nine clones were highly homologous with known genes. Of the remaining seven cDNA clones, five clones matched with known expressed sequence tag (EST) sequences from wheat and (or) barley whereas the other two clones were unknown. Five of the nine differentially expressed sequences (WTJ9, WTJ11, WTJ15, WTJ19, and WTJ32) were highly homologous (identities >94%) with ESTs from wheat or barley challenged with pathogens. These five sequences and another one (WTJ18) were also highly homologous (identities >86%) with abiotic stress induced ESTs in wheat or barley. Reverse Northern hybridization showed that seven of the nine differentially expressed cDNA sequences hybridized with cDNA of T. intermedium infected by BYDV. Three of these also hybridized with cDNA of line 3B-2 (a parent of TAI-27) infected by BYDV. The alien chromosome in TAI-27 was microdissected. The second round linker adaptor mediated PCR products of the alien chromosomal DNA were labeled with digoxygenin and used as the probe to hybridize with the nine differentially expressed genes. The analysis showed that seven differentially expressed genes were homologous with the alien chromosome of TAI-27. These seven differentially expressed sequences could be used as ESTs of the alien chromosome of TAI-27. This research laid the foundation for screening and cloning of new specific functional genes conferring resistance to BYDV and probably other pathogens.Key words: suppression subtractive hybridization (SSH), expressed sequence tag (EST), linker adaptor mediated polymerase chain reaction (LA-PCR), chromosome microdissection.

Molecular cytogenetic discrimination and reaction to wheat streak mosaic virus and the wheat curl mite in Zhong series of wheat – Thinopyrum intermedium partial amphiploids

Genome ◽  
2003 ◽  
Vol 46 (1) ◽  
pp. 135-145 ◽  
Author(s):  
Qin Chen ◽  
R L Conner ◽  
H J Li ◽  
S C Sun ◽  
F Ahmad ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Barley Yellow Dwarf Virus ◽  
Wheat Breeding ◽  
Wheat Streak Mosaic Virus ◽  
The Other ◽  
Thinopyrum Intermedium ◽  
Wheat Curl Mite ◽  
Partial Amphiploid ◽  
Yellow Dwarf Virus ◽  
Partial Amphiploids

Thinopyrum intermedium (2n = 6x = 42, JJJsJsSS) is potentially a useful source of resistance to wheat streak mosaic virus (WSMV) and its vector, the wheat curl mite (WCM). Five partial amphiploids, namely Zhong 1, Zhong 2, Zhong 3, Zhong 4, and Zhong 5, derived from Triticum aestivum × Thinopyrum intermedium crosses produced in China, were screened for WSMV and WCM resistance. Zhong 1 and Zhong 2 had high levels of resistance to WSMV and WCM. The other three partial amphiploids, Zhong 3, 4, and 5, were resistant to WSMV, but were susceptible to WCM. Genomic in situ hybridization (GISH) using a genomic DNA probe from Pseudoroegneria strigosa (SS, 2n = 14) demonstrated that two partial amphiploids, Zhong 1 and Zhong 2, have almost the identical 10 Th. intermedium chromosomes, including four Js, four J, and two S genome chromosomes. Both of them carry two pairs of J and a pair of Js genome chromosomes and two different translocations that were not observed in the other three Zhong lines. The partial amphiploids Zhong 3, 4, and 5 have another type of basic genomic composition, which is similar to a reconstituted alien genome consisting of four S and four Js genome chromosomes of Th. intermedium (Zhong 5 has two Js chromosomes plus two Js–W translocations) with six translocated chromosomes between S and Js or J genomes. All three lines carry a specific S–S–Js translocated chromosome, which might confer resistance to barley yellow dwarf virus (BYDV-PAV). The present study identified a specific Js2 chromosome present in all five of the Zhong lines, confirming that a Js chromosome carries WSMV resistance. Resistance to WCM may be linked with J or Js chromosomes. The discovery of high levels of resistance to both WSMV and WCM in Zhong 1 and Zhong 2 offers a useful source of resistance to both the virus and its vector for wheat breeding programs.Key words: GISH, genomic composition, J, Js and S genomes, Thinopyrum intermedium, partial amphiploid, WSMV, WCM resistance.

Isolation of expressed sequences from a specific chromosome of Thinopyrum intermedium infected by BYDV

Genome ◽  
2009 ◽  
Vol 52 (1) ◽  
pp. 68-76 ◽  
Author(s):  
Shu-Mei Jiang ◽  
Wei-Bo Yin ◽  
Jun Hu ◽  
Rui Shi ◽  
Ruo-Nan Zhou ◽  
...  
Keyword(s):  
Large Scale ◽  
Barley Yellow Dwarf Virus ◽  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Addition Line ◽  
Alien Chromosome ◽  
Thinopyrum Intermedium ◽  
Specific Chromosome ◽  
Barley Yellow Dwarf ◽  
Yellow Dwarf Virus

To map important ESTs to specific chromosomes and (or) chromosomal regions is difficult in hexaploid wheat because of its large genome size and serious interference of homoeologous sequences. Large-scale EST sequencing and subsequent chromosome localization are both laborious and time-consuming. The wheat alien addition line TAi-27 contains a pair of chromosomes of Thinopyrum intermedium (Host) Barkworth & D.R. Dewey that carry the resistance gene against barley yellow dwarf virus. In this research, we developed a modified technique based on chromosome microdissection and hybridization-specific amplification to isolate expressed sequences from the alien chromosome of TAi-27 by hybridization between the DNA of the microdissected alien chromosome and cDNA of Th. intermedium infected by barley yellow dwarf virus. Twelve clones were selected, sequenced, and analyzed. Three of them were unknown genes without any hit in the GenBank database and the other nine were highly homologous with ESTs of wheat, barley, and (or) other plants in Gramineae induced by abiotic or biotic stress. The method used in this research to isolate expressed sequences from a specific chromosome has the following advantages: (i) the obtained expressed sequences are larger in size and have 3′ end information and (ii) the operation is less complicated. It would be an efficient improved method for genomics and functional genomics research of polyploid plants, especially for EST development and mapping. The obtained expressed sequence data are also informative in understanding the resistance genes on the alien chromosome of TAi-27.

Survey of Barley yellow dwarf virus incidence in winter cereal crops, and assessment of wheat and barley resistance to the virus

2016 ◽  
Vol 67 (10) ◽  
pp. 1054 ◽  
Author(s):  
Eva Beoni ◽  
Jana Chrpová ◽  
Jana Jarošová ◽  
Jiban Kumar Kundu
Keyword(s):  
Barley Yellow Dwarf Virus ◽  
Rhopalosiphum Padi ◽  
Wheat Breeding ◽  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Cereal Crops ◽  
Winter Cereal ◽  
Barley Yellow Dwarf ◽  
Significant Difference ◽  
Yellow Dwarf Virus

A survey of Barley yellow dwarf virus (BYDV) incidence in cereal crops in the Czech Republic over 4 years showed, on average, 13.3% BYDV-positive, randomly tested wheat and barley samples. The cultivated wheat and barley cultivars had different levels of susceptibility to BYDV infection. Field trials were performed with different barley and wheat breeding lines and cultivars, and resistance traits were evaluated after artificial inculcation by the viruliferous aphid vector Rhopalosiphum padi L. with BYDV-PAV. Our results showed high variability of visual symptom score (VSS) and reduction in grain weight per spike (GWS-R) in trials within the tested lines and cultivars. The barley line (WBON 96-123) and cultivars (Wysor, Travira) that contained RYd2 differed significantly from other cultivars in VSS. Line WBON 96-123 and cvv. Wysor and Yatzi showed the lowest GWS-R. Wheat line PSR 3628 and cvv. Altigo, Elan, Sparta, Aladin and Hewit showed significant difference from other cultivars in VSS. PSR 3628, Sparta, and Elan showed the lowest GWS-R. Similar results were obtained from BYDV titre analysis by RT-qPCR corresponding to the VSS and GWS-R traits. A low virus titre corresponded to low VSS and GWS-R. Hence, our results suggest that laborious and time-consuming GWS-R analysis could be replaced in some cases by qPCR-based BYDV titre analysis and, together with VSS evaluation, could enhance the efficiency of resistance assessment.

Disomic Thinopyrum intermedium addition lines in wheat with barley yellow dwarf virus resistance and with rust resistances

Genome ◽  
1995 ◽  
Vol 38 (2) ◽  
pp. 385-394 ◽  
Author(s):  
P. J. Larkin ◽  
P. M. Banks ◽  
E. S. Lagudah ◽  
R. Appels ◽  
Chen Xiao ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Stem Rust ◽  
Rust Resistance ◽  
Barley Yellow Dwarf Virus ◽  
Yellow Dwarf ◽  
Addition Lines ◽  
Thinopyrum Intermedium ◽  
Barley Yellow Dwarf ◽  
Group 2 ◽  
Yellow Dwarf Virus

Zhong 5 is a partial amphiploid (2n = 56) between Triticum aestivum (2n = 42) and Thinopyrum intermedium (2n = 42) carrying all the chromosomes of wheat and seven pairs of chromosomes from Th. intermedium. Following further backcrossing to wheat, six independent stable 2n = 44 lines were obtained representing 4 disomic chromosome addition lines. One chromosome confers barley yellow dwarf virus (BYDV) resistance, whereas two other chromosomes carry leaf and stem rust resistance; one of the latter also confers stripe rust resistance. Using RFLP and isozyme markers we have shown that the extra chromosome in the Zhong 5-derived BYDV resistant disomic addition lines (Z1, Z2, or Z6) belongs to the homoeologous group 2. It therefore carries a different locus to the BYDV resistant group 7 addition, L1, described previously. The leaf, stem, and stripe rust resistant line (Z4) carries an added group 7 chromosome. The line Z3 has neither BYDV nor rust resistance, is not a group 2 or group 7 addition, and is probably a group 1 addition. The line Z5 is leaf and stem rust resistant, is not stripe rust resistant, and its homoeology remains unknown.Key words: Agropyron, intermediate wheatgrass, leaf rust, stem rust, stripe rust, luteovirus.

Implementation of probes for tracing chromosome segments conferring barley yellow dwarf virus resistance

2001 ◽  
Vol 52 (12) ◽  
pp. 1389 ◽  
Author(s):  
W. Zhang ◽  
M. Carter ◽  
S. Matsay ◽  
P. Stoutjesdijk ◽  
R. Potter ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Barley Yellow Dwarf Virus ◽  
Solid Phase ◽  
Wheat Breeding ◽  
Yellow Dwarf ◽  
Chromosome Segment ◽  
Dwarf Virus ◽  
Barley Yellow Dwarf ◽  
Wheat Group ◽  
Yellow Dwarf Virus

Two PCR-based assays were examined for tracing the presence of a Thinopyrum chromosome segment (Tc6 or Tc14) conferring barley yellow dwarf virus (BYDV) resistance in wheat breeding lines. The microsatellite gwm37 was used to assay the Thinopyrum chromosome segment or its wheat, Group 7, homoeologous segment, and was effective in characterising breeders material since heterozygous lines could be identified. A new set of primers derived from a Thinopyrum-specific DNA segment (csTiB1) provided a dominant marker that was readily scored by agarose gel electrophoresis. It was also demonstrated that the csTiB1 primers could be used to establish a solid phase PCR assay that avoided the requirement for gel electrophoresis and was amenable to use in a high-throughput, microtitre plate format. Depending on the number of DNA samples to be assayed, both primer pairs appear to have a place in breeding programs.

Varying chromosome composition of 56-chromosome wheat × Thinopyrum intermedium partial amphiploids

Genome ◽  
1993 ◽  
Vol 36 (2) ◽  
pp. 207-215 ◽  
Author(s):  
P. M. Banks ◽  
S. J. Xu ◽  
R. R.-C. Wang ◽  
P. J. Larkin
Keyword(s):  
Barley Yellow Dwarf Virus ◽  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Molecular Evidence ◽  
Thinopyrum Intermedium ◽  
Meiotic Analysis ◽  
Barley Yellow Dwarf ◽  
Yellow Dwarf Virus ◽  
Head Morphology ◽  
Partial Amphiploids

Thinopyrum intermedium (2n = 42) is a source of many potentially useful genes for wheat improvement. Many partial amphiploids have been produced between Th. intermedium and Triticum aestivum that are fertile and stable. These partial amphiploids all have 56 chromosomes, including seven pairs of chromosomes from Th. intermedium. To explore the genomic composition of these lines, meiotic analysis was conducted on 32 hybrid combinations between eight different partial amphiploids. All but two of the chosen parents were distinguishable on the basis of perenniality, head morphology, and reactions to leaf, stripe, and stem rusts and to barley yellow dwarf virus. Chromosome pairing in the hybrids clearly indicated that all but two of the partial amphiploids differed in their composition of Thinopyrum chromosomes. The differences varied from one to five chromosomes. This confirms molecular evidence that the extra genome of the octoploid partial amphiploids is a variable synthetic genome combining chromosomes of the three Thinopyrum genomes E, J, and X. Though the extra synthetic genomes vary widely between different octoploids, they are nevertheless stable once formed. It is argued that the failure to establish these octoploid amphiploids as a new crop is a consequence of their differing chromosome complements, which makes it impractical to interbreed them.Key words: Thinopyrum, Agropyron, agrotriticum, wheat, amphiploid, octoploid, barley yellow dwarf virus, rust.

scholarly journals Theoretical Bases and Sources for Breeding Wheat for Combined Disease Resistance

2011 ◽  
Vol 41 (No. 4) ◽  
pp. 127-143 ◽  
Author(s):  
V. Šíp ◽  
P. Bartoš ◽  
J. Chrpová ◽  
A. Hanzalová ◽  
L. Širlová ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Barley Yellow Dwarf Virus ◽  
Wheat Breeding ◽  
Tan Spot ◽  
Common Bunt ◽  
Head Blight ◽  
Sources Of Resistance ◽  
Barley Yellow Dwarf ◽  
Glume Blotch ◽  
Yellow Dwarf Virus

Achievements and prospects of wheat breeding for disease resistance in the world and in theCzech Republic are discussed. Attention was paid to possibilities of increasing resistance to rusts, powdery mildew, Fusarium head blight, leaf blotch, glume blotch, tan spot, common bunt and barley yellow dwarf virus on wheat. Methodical approaches adopted in national ring infection tests were outlined. New sources of resistance to the above-mentioned diseases were detected and described on the basis of three-year results of field infection tests.  

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