Genetic purity analysis of hybrid broccoli (Brassica oleracea var. italica) seeds using RAPD PCR

2002 ◽  
Vol 53 (1) ◽  
pp. 51 ◽  
Author(s):  
Phillip A. Crockett ◽  
Mohan B. Singh ◽  
C. K. Lee ◽  
Prem L Bhalla
Keyword(s):  
Plant Size ◽  
Vegetable Crops ◽  
Genetic Purity ◽  
F1 Hybrid ◽  
Shoot Ratio ◽  
Root Shoot Ratio ◽  
Rapd Pcr ◽  
Purity Analysis ◽  
Hybrid Seeds ◽  
Contrast Response

Determination of genetic purity of F1-hybrid seeds is aquality control requirement in the production of hybrid Brassica vegetableseeds. Hybrid varieties of these vegetable crops have arisen from a limitedgermplasm base, making discrimination of parental and hybrid lines verylaborious and troublesome. The use of RAPD PCR for evaluating seed purity in acommercial F1-hybrid broccoli a single cultivar bywithholding water. In Expt 2, plants of EP and MK were grown together in thesame container and received water daily with gradation in intensity of waterdeficit achieved by varying the daily water ration per container.All cultivars in each experiment exhibited commonly reported responses towater deficit, characterised by diminished evaporative surface area andincreased root : shoot ratio. The response of MK was primarily morphologicaland MK plants had smaller plant size, higher root : shoot ratio, and a lowergrowth rate compared with temperate cultivars. By contrast, response oftemperate cultivars was primarily physiological; stomatal conductance oftemperate cultivars was lower and these cultivars had a greater tendency forleaf lamina osmotic a

Effects of water deficit on Mediterranean and temperate cultivars of tall fescue

2002 ◽  
Vol 53 (1) ◽  
pp. 29 ◽  
Author(s):  
S. G. Assuero ◽  
C. Matthew ◽  
P. Kemp ◽  
D. J. Barker ◽  
the late A. Mazzanti
Keyword(s):  
Water Deficit ◽  
Tall Fescue ◽  
Festuca Arundinacea ◽  
Plant Size ◽  
Lower Growth ◽  
Shoot Ratio ◽  
Root Shoot Ratio ◽  
Morphological Adaptations ◽  
Lower Growth Rate ◽  
Contrast Response

Two glasshouse experiments were carried out to evaluate the morphological and physiological responses to water deficit of Mediterranean and temperate tall fescue (Festuca arundinacea Schreb.) cultivars. In Expt 1, 3 cultivars were studied: 2 temperate cultivars, Grasslands Advance (GA) and El Palenque (EP); and a Mediterranean cultivar, Maris Kasba (MK). Water deficit was induced in containers of 4 plants of a single cultivar by withholding water. In Expt 2, plants of EP and MK were grown together in the same container and received water daily with gradation in intensity of water deficit achieved by varying the daily water ration per container. All cultivars in each experiment exhibited commonly reported responses to water deficit, characterised by diminished evaporative surface area and increased root : shoot ratio. The response of MK was primarily morphological and MK plants had smaller plant size, higher root : shoot ratio, and a lower growth rate compared with temperate cultivars. By contrast, response of temperate cultivars was primarily physiological; stomatal conductance of temperate cultivars was lower and these cultivars had a greater tendency for leaf lamina osmotic adjustment than MK under the most stressful water deficit conditions studied. The morphological adaptations of MK were very effective in delaying the onset of water deficit in Expt 1 when plants were grown with others of the same cultivar, but rendered MK plants uncompetitive and more susceptible to water deficit in Expt 2 where plants of MK where interspersed with plants of a temperate cultivar.

scholarly journals Isolation and Identification of Molecular Markers for Fingerprinting of Chilli Hybrids & its Parental Lines

2020 ◽  
Vol 11 (1) ◽  
pp. 713-716
Author(s):  
Shishir Tiwari ◽  
Shweta Sao ◽  
Antu Kurrey ◽  
Pulak Das
Keyword(s):  
Molecular Markers ◽  
Issr Marker ◽  
Genetic Purity ◽  
Isolation And Identification ◽  
Rapd Primer ◽  
Purity Analysis ◽  
Hybrid Seeds ◽  
Environmentally Sensitive ◽  
Parental Lines ◽  
Morphological Differences

Chilli (Capsicum annum) is the predominant sp., which is cultivated in both hot and sweet papers. The maintenance of the genetic purity of chilli plant is a matter of great concern for the breeders. For genetic purity analysis, between true hybrids and off-types, breeders find out morphological differences between them, but this technique is cannot be recognized easily and also costly, tedious to score, and environmentally sensitive. Alternatively,  molecular markers based genetic purity analysis can be employed. The molecular marker-based technique was thus used to overcome the conventional method drawbacks. The main objective of the study is to identify informative molecular markers (ISSR and RAPD) capable of distinguishing Chilli hybrids and their parental lines and their utilization in seed purity assessment. Five parental lines of Chilli (i.eCH10, CH12, CH530, CH709, CH734) were used for the production of 3 hybrids. Total 30 ISSR and 8 RAPD primers were selected for the study of 5 parental lines, among them 2ISSR and 1 RAPD primers produced unique fingerprinting across the hybrids. The ISSR marker UBC815 amplified alleles specific to different parental lines(CH10 & CH12) for hybrids (ACH112), The ISSR marker UBC 827, amplified alleles specific to different parental lines(CH709 & CH12) for hybrids (ACH179). Likewise, RAPD primer B20 for hybrid ACH 753 and their parental lines(CH734 & CH530). Thus, the above study showed that the aid of molecular markers is more reliable, highly efficient, and reproducible for assessing fingerprinting of Chilli commercial hybrid seeds with more accuracy.

scholarly journals The production and storage of a fermentor-grown bacterial culture containing Synergistes jonesii, for protecting cattle against mimosine and 3-hydroxy-4(1H)-pyridone toxicity from feeding on Leucaena leucocephala

2002 ◽  
Vol 53 (1) ◽  
pp. 1 ◽  
Author(s):  
A. V. Klieve ◽  
D. Ouwerkerk ◽  
A. Turner ◽  
R. Roberton
Keyword(s):  
Amino Acid ◽  
Leucaena Leucocephala ◽  
Bacterial Culture ◽  
Plant Size ◽  
Tropical Agriculture ◽  
Shoot Ratio ◽  
Root Shoot Ratio ◽  
Forage Species ◽  
Daily Water ◽  
And Storage

The leguminous shrub Leucaena leucocephala (leucaena) iswidely used as a forage species for cattle in tropical agriculture. However,leucaena contains the toxic amino acid mimosine. Both mimosine and its primaryruminal degradation product 3-hydroxy-4(1H)-pyridone (DHP) are toxic and theiraccumulation in the animal’s system results in hair loss, reducedliveweight gain, and goitre. The ruminal bacteriumngle cultivar bywithholding water. In Expt 2, plants of EP and MK were grown together in thesame container and received water daily with gradation in intensity of waterdeficit achieved by varying the daily water ration per container.All cultivars in each experiment exhibited commonly reported responses towater deficit, characterised by diminished evaporative surface area andincreased root : shoot ratio. The response of MK was primarily morphologicaland MK plants had smaller plant size, higher root : shoot ratio,

scholarly journals Evaluation of Genetic Purity of F1 Hybrid Seeds in Cabbage with RAPD, ISSR, SRAP, and SSR Markers

HortScience ◽  
2007 ◽  
Vol 42 (3) ◽  
pp. 724-727 ◽  
Author(s):  
Liwang Liu ◽  
Guang Liu ◽  
Yiqin Gong ◽  
Wenhao Dai ◽  
Yan Wang ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Genetic Relationships ◽  
Random Amplified Polymorphic Dna ◽  
Female Parent ◽  
Sequence Repeat ◽  
Genetic Purity ◽  
F1 Hybrid ◽  
Ssr Primers ◽  
Hybrid Seeds ◽  
Issr Primers

Four molecular marker systems—RAPD (random amplified polymorphic DNA), ISSR (intersimple sequence repeat), SRAP (sequence-related amplified polymorphism), and SSR (simple sequence repeat)—were used to evaluate seed genetic purity of a hybrid cabbage cultivar ‘Zaoxia 16’. Genetic relationships of the F1 hybrids and their parents were analyzed with 157 RAPD primers, 54 ISSR primers, 84 SRAP primer combinations, and 44 SSR primers. Three RAPD primers (NAURP2006, NAURP2020, and NAURP2031), two ISSR primers (NAUISR1058 and NAUISR1062), one SRAP primer combination (NAUSR04/NAURS05), and two SSR primers (NAUSSR1011 and NAUSSR1031), which produced male and female parent-specific markers simultaneously, were selected for testing the genetic purity of the F1 seeds. A total of 210 ‘Zaoxia 16’ hybrid individuals were investigated with these eight selected primers. Of these, 12 appeared to be false hybrids. Nine of the 12 putative false hybrids, confirmed with all eight primers, exhibited similar banding patterns to the female parent, suggesting that they could be derived from selfing of the female parent. The results were in accordance with those from field evaluations. This study showed that RAPD, ISSR, SRAP, and SSR markers are highly efficient and reproducible for genetic purity testing of cabbage commercial hybrid seeds.

Effects of Single-seed Sowing on Root Growth, Root-shoot Ratio, and Yield inPeanut (Arachis hypogacaL.)

2013 ◽  
Vol 39 (12) ◽  
pp. 2228 ◽  
Author(s):  
Ye FENG ◽  
Feng GUO ◽  
Bao-Long LI ◽  
Jing-Jing MENG ◽  
Xin-Guo LI ◽  
...  
Keyword(s):  
Root Growth ◽  
Single Seed ◽  
Shoot Ratio ◽  
Root Shoot Ratio ◽  
Seed Sowing

Root-shoot ratio in irrigated plants

1965 ◽  
Vol 7 (2) ◽  
pp. 129-135 ◽  
Author(s):  
Miroslav Penka
Keyword(s):  
Shoot Ratio ◽  
Root Shoot Ratio
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