Stable transformation ofRhizoctonia solaniwith a modified hygromycin resistance gene

2009 ◽  
Vol 38 (1) ◽  
pp. 79 ◽  
Author(s):  
Jiang Wu ◽  
Philip A. O' Brien
Keyword(s):  
Resistance Gene ◽  
Stable Transformation ◽  
Hygromycin Resistance ◽  
Hygromycin Resistance Gene

A chimaeric hygromycin resistance gene as a selectable marker in plant cells

1985 ◽  
Vol 5 (5) ◽  
pp. 299-302 ◽  
Author(s):  
Peter J. M. van den Elzen ◽  
Jeffrey Townsend ◽  
Kathleen Y. Lee ◽  
John R. Bedbrook
Keyword(s):  
Resistance Gene ◽  
Selectable Marker ◽  
Plant Cells ◽  
Hygromycin Resistance ◽  
Hygromycin Resistance Gene

A mutated hygromycin resistance gene is functional in the n -alkane-assimilating yeast Candida tropicalis

2000 ◽  
Vol 173 (3) ◽  
pp. 187-192 ◽  
Author(s):  
Akihiro Hara ◽  
Mitsuyoshi Ueda ◽  
Satoru Misawa ◽  
Toru Matsui ◽  
Keizo Furuhashi ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Candida Tropicalis ◽  
Hygromycin Resistance ◽  
Hygromycin Resistance Gene

scholarly journals Hygromycin Resistance Gene Cassettes for Vector Construction and Selection of Transformed Rice Protoplasts

1991 ◽  
Vol 97 (2) ◽  
pp. 832-835 ◽  
Author(s):  
Zhenwei Zheng ◽  
Akio Hayashimoto ◽  
Zhijian Li ◽  
Norimoto Murai
Keyword(s):  
Resistance Gene ◽  
Hygromycin Resistance ◽  
Gene Cassettes ◽  
Vector Construction ◽  
Hygromycin Resistance Gene ◽  
Selection Of

Transformation of Aspergillus nidulans with the hygromycin-resistance gene, hph

Gene ◽  
1987 ◽  
Vol 57 (1) ◽  
pp. 21-26 ◽  
Author(s):  
D. Cullen ◽  
S.A. Leong ◽  
L.J. Wilson ◽  
D.J. Henner
Keyword(s):  
Aspergillus Nidulans ◽  
Resistance Gene ◽  
Hygromycin Resistance ◽  
Hygromycin Resistance Gene

scholarly journals Stability and modulated expression of a hygromycin resistance gene integrated in Botrytis cinerea transformants

2006 ◽  
Vol 154 (2) ◽  
pp. 187-193 ◽  
Author(s):  
Walid Hamada ◽  
Marie-Christine Soulié ◽  
Pierrette Malfatti ◽  
Gilbert Bompeix ◽  
Martine Boccara
Keyword(s):  
Botrytis Cinerea ◽  
Resistance Gene ◽  
Hygromycin Resistance ◽  
Hygromycin Resistance Gene

scholarly journals Transfer of a Supernumerary Chromosome Between Vegetatively Incompatible Biotypes of the Fungus Colletotrichum gloeosporioides

Genetics ◽  
1998 ◽  
Vol 150 (4) ◽  
pp. 1459-1466 ◽  
Author(s):  
Chaozu He ◽  
Anca G Rusu ◽  
Agnieszka M Poplawski ◽  
John A G Irwin ◽  
John M Manners
Keyword(s):  
Resistance Gene ◽  
Colletotrichum Gloeosporioides ◽  
Mixed Cultures ◽  
Marker Genes ◽  
Hygromycin Resistance ◽  
Antibiotic Resistant ◽  
Selectable Marker Genes ◽  
Tropical Legumes ◽  
Hygromycin Resistance Gene ◽  
Biotype B

Abstract Two biotypes (A and B) of Colletotrichum gloeosporioides infect the tropical legumes Stylosanthes spp. in Australia. These biotypes are asexual and vegetatively incompatible. However, field isolates of biotype B carrying a supernumerary 2-Mb chromosome, thought to originate from biotype A, have been reported previously. We tested the hypothesis that the 2-Mb chromosome could be transferred from biotype A to biotype B under laboratory conditions. Selectable marker genes conferring resistance to hygromycin and phleomycin were introduced into isolates of biotypes A and B, respectively. A transformant of biotype A, with the hygromycin resistance gene integrated on the 2-Mb chromosome, was cocultivated with phleomycin-resistant transformants of biotype B. Double antibiotic-resistant colonies were obtained from conidia of these mixed cultures at a frequency of approximately 10-7. Molecular analysis using RFLPs, RAPDs, and electrophoretic karyotypes showed that these colonies contained the 2-Mb chromosome in a biotype B genetic background. In contrast, no double antibiotic colonies developed from conidia obtained from mixed cultures of phleomycin-resistant transformants of biotype B with biotype A transformants carrying the hygromycin resistance gene integrated in chromosomes >2 Mb in size. The results demonstrated that the 2-Mb chromosome was selectively transferred from biotype A to biotype B. The horizontal transfer of specific chromosomes across vegetative incompatibility barriers may explain the origin of supernumerary chromosomes in fungi.

Sign in / Sign up

Export Citation Format

Share Document