Comparison of aquaporin-1 expression between yak (Bos grunniens) and indigenous cattle (Bos taurus) in the Qinghai–Tibetan Plateau

2017 ◽  
Vol 57 (8) ◽  
pp. 1618 ◽  
Author(s):  
C. L. Zhong ◽  
J. P. Kang ◽  
G. S. Stewart ◽  
J. W. Zhou ◽  
X. D. Huang ◽  
...  
Keyword(s):  
Tibetan Plateau ◽  
Parotid Gland ◽  
Bos Taurus ◽  
Large Family ◽  
Outer Cortex ◽  
Indigenous Cattle ◽  
Aquaporin 1 ◽  
Bos Grunniens ◽  
New Perspective ◽  
Species Specific

Aquaporins (AQPs) are a large family of integral membrane proteins that facilitate the transport of water through the biomembranes. AQP1, one of the 13 AQPs identified in mammals, is distributed in various tissues and organs, and plays an important role in body water homeostasis. The objectives of the present study were to identify the expression of aquaporin-1 (AQP1) in the kidney, rumen and parotid gland of yaks, and to quantify whether the protein abundance of AQP1 is species specific between yak (Bos grunniens) and indigenous cattle (Bos taurus). Three 3-year-old castrated males (156 ± 6.8 kg of bodyweight) of each of three genotypes, namely, yak, indigenous cattle and the crossbred between the two (Bos taurus♂ × Bos grunniens♀), were used; all animals were grazed in the same autumn pasture of the Qinghai–Tibetan Plateau, China. Western blot results detected (1) 28-kDa unglycosylated AQP1 in the kidney, rumen and parotid gland of all three genotypes, 40-kDa glycosylated AQP1 in renal cortex and medulla. (2) Yaks expressed less 28-kDa AQP1 protein in the outer cortex (P < 0.05), significantly more in the outer medulla (P < 0.01), and slightly more in the ventral rumen (P = 0.088) than did cattle. No difference was observed in the dorsal rumen and parotid gland (P > 0.10). In conclusion, the present study is the first to confirm the presence of AQP1 in bovine rumen and parotid gland and identified its expression in yaks. Abundance of AQP1 protein in yak kidney showed some difference from indigenous cattle. This could provide a new perspective to explain some adaptive mechanisms of yaks to the harsh environment in the Qinghai–Tibetan Plateau.

scholarly journals Comparison of Nitrogen Metabolism in Yak (Bos grunniens) and Indigenous Cattle (Bos taurus) on the Qinghai-Tibetan Plateau

2011 ◽  
Vol 24 (6) ◽  
pp. 766-773 ◽  
Author(s):  
Hucheng Wang ◽  
Ruijun Long ◽  
Juan Boo Liang ◽  
Xusheng Guo ◽  
Luming Ding ◽  
...  
Keyword(s):  
Tibetan Plateau ◽  
Nitrogen Metabolism ◽  
Bos Taurus ◽  
Indigenous Cattle ◽  
Bos Grunniens

Comparison of nitrogen utilization and urea kinetics between yaks (Bos grunniens) and indigenous cattle (Bos taurus)1

2017 ◽  
Vol 95 (10) ◽  
pp. 4600-4612 ◽  
Author(s):  
J. W. Zhou ◽  
C. L. Zhong ◽  
H. Liu ◽  
A. A. Degen ◽  
E. C. Titgemeyer ◽  
...  
Keyword(s):  
Bos Taurus ◽  
Nitrogen Utilization ◽  
Indigenous Cattle ◽  
Bos Grunniens ◽  
Urea Kinetics

scholarly journals The rumen microbiome of yak co-evolves with its host probably adding the adaptation to its harsh environments

2021 ◽  
Author(s):  
Congcong Zhao ◽  
Lamei Wang ◽  
Shanlin Ke ◽  
Xinhua Chen ◽  
Ákos Kenéz ◽  
...  
Keyword(s):  
Tibetan Plateau ◽  
Bos Taurus ◽  
Glycoside Hydrolases ◽  
Rumen Microbiota ◽  
Rumen Microbes ◽  
Bos Grunniens ◽  
Molar Proportion ◽  
Rumen Microbiome ◽  
Sufficient Energy ◽  
Genes Encoding

Abstract Background Rumen microbes play an important role in ruminant energy supply and animal performance. Previous studies showed that yak (Bos grunniens) rumen microbiome and fermentation differ from other ruminants. However, little is understood on the features of the rumen microbiome that make yak adapted to its unique environmental and dietary conditions. Here we investigated the rumen microbiome and metabolome to understand how yak adapts to the coarse forage and harsh environment in the high Qinghai-Tibetan plateau. Result Metataxonomic analysis of the rumen microbiota revealed that yak (Bos grunniens), domesticated cattle (Bos taurus), and dzo (a hybrid between the yak and domestic cattle) have distinct rumen microbiota. Metagenomic analysis displayed a larger gene pool encoding a richer repertoire of carbohydrate-active enzymes (CAZymes) in the rumen microbiome of yak and dzo than cattle. Some of the genes encoding glycoside hydrolases (GH) that mediate the digestion of cellulose and hemicellulose were significantly enriched in the rumen of yak than cattle, but the cattle rumen microbiome had more genes assigned to GH57 that primarily includes amylases. The rumen fermentation profile differed also, with cattle having a higher molar proportion of acetate but a lower molar proportion of propionate than dzo and yak. Metabolomic analysis showed differences in both rumen microbial metabolic pathways and metabolites, mainly amino acids, carboxylic acids, sugars, and bile acids. Notably, styrene degradation, primary bile acid biosynthesis, glyoxylate, and dicarboxylate metabolism significantly differed between cattle and dzo; streptomycin biosynthesis was significantly different between cattle and yak; and the pathways for biotin metabolism and styrene degradation significantly differed between dzo and yak. Correlation analysis revealed certain microbial species correlated with differential rumen metabolites. Nine differential metabolites showed a positive correlation with seven species belonging to Bacteroides and Alistipes but a negative correlation with ten species belonging to Prevotella and Ruminococcus. Conclusion The present study showed that the rumen microbiome of yak and its host had probably co-evolved aiding in the adaptation of yak to the harsh dietary environment of the Qinghai-Tibetan plateau. In particular, the yak rumen microbiome has more enzymes involved in the degradation of rough forage than that of cattle, providing sufficient energy for its host.

scholarly journals Comparison of methanogen diversity of yak (Bos grunniens) and cattle (Bos taurus) from the Qinghai-Tibetan plateau, China

2012 ◽  
Vol 12 (1) ◽  
pp. 237 ◽  
Author(s):  
Xiao Huang ◽  
Hui Tan ◽  
Ruijun Long ◽  
Juan Liang ◽  
André-Denis G Wright
Keyword(s):  
Tibetan Plateau ◽  
Bos Taurus ◽  
Bos Grunniens

scholarly journals In Search of Species-Specific SNPs in a Non-Model Animal (European Bison (Bison bonasus))—Comparison of De Novo and Reference-Based Integrated Pipeline of STACKS Using Genotyping-by-Sequencing (GBS) Data

Animals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 2226
Author(s):  
Sazia Kunvar ◽  
Sylwia Czarnomska ◽  
Cino Pertoldi ◽  
Małgorzata Tokarska
Keyword(s):  
Reference Genome ◽  
De Novo ◽  
Bos Taurus ◽  
Model Organism ◽  
Genotyping By Sequencing ◽  
Model Organisms ◽  
European Bison ◽  
Model Animal ◽  
Pcr Duplicates ◽  
Species Specific

The European bison is a non-model organism; thus, most of its genetic and genomic analyses have been performed using cattle-specific resources, such as BovineSNP50 BeadChip or Illumina Bovine 800 K HD Bead Chip. The problem with non-specific tools is the potential loss of evolutionary diversified information (ascertainment bias) and species-specific markers. Here, we have used a genotyping-by-sequencing (GBS) approach for genotyping 256 samples from the European bison population in Bialowieza Forest (Poland) and performed an analysis using two integrated pipelines of the STACKS software: one is de novo (without reference genome) and the other is a reference pipeline (with reference genome). Moreover, we used a reference pipeline with two different genomes, i.e., Bos taurus and European bison. Genotyping by sequencing (GBS) is a useful tool for SNP genotyping in non-model organisms due to its cost effectiveness. Our results support GBS with a reference pipeline without PCR duplicates as a powerful approach for studying the population structure and genotyping data of non-model organisms. We found more polymorphic markers in the reference pipeline in comparison to the de novo pipeline. The decreased number of SNPs from the de novo pipeline could be due to the extremely low level of heterozygosity in European bison. It has been confirmed that all the de novo/Bos taurus and Bos taurus reference pipeline obtained SNPs were unique and not included in 800 K BovineHD BeadChip.

Haematobia irritans parasitism of F1 yak × beef cattle ( Bos grunniens  ×  Bos taurus ) hybrids

2019 ◽  
Vol 33 (4) ◽  
pp. 546-551 ◽  
Author(s):  
C. M. Calkins ◽  
J. D. Scasta ◽  
T. Smith ◽  
M. M. Stayton ◽  
S. L. Lake
Keyword(s):  
Beef Cattle ◽  
Bos Taurus ◽  
Haematobia Irritans ◽  
Bos Grunniens
Sign in / Sign up

Export Citation Format

Share Document