Effect of dietary Lippia citriodora extract on reproductive and productive performance and plasma biochemical parameters in rabbit does

2017 ◽  
Vol 57 (1) ◽  
pp. 65 ◽  
Author(s):  
D. Casamassima ◽  
M. Palazzo ◽  
F. Vizzarri ◽  
L. Ondruska ◽  
P. Massanyi ◽  
...  
Keyword(s):  
Dietary Supplement ◽  
Biochemical Parameters ◽  
Density Lipoprotein ◽  
Dose Effect ◽  
Lipoprotein Cholesterol ◽  
Daily Weight Gain ◽  
Reactive Oxygen Metabolites ◽  
Adaptation Period ◽  
Natural Extract ◽  
Lippia Citriodora

Two doses of natural extract from Lippia citriodora (titrated in verbascoside) were assessed in New Zealand White rabbit does evaluating selected reproductive, productive and plasma biochemical parameters. After 1 week of adaptation period, the trial on 45 rabbit does for three consecutive reproductive cycles was conducted; does were divided into three groups of 15 animals each, homogenous by age (1 year ± 2 weeks), bodyweight (4.77 ± 0.21 kg) and parity (2 ± 1). A control group (CON) did not receive the dietary supplement in the feed and the other two groups received 1 g of natural extract supplement in the feed (5 mg verbascoside/kg feed; LNE) and 2 g of natural extract (10 mg verbascoside/kg feed; HNE). The use of a dietary NE supplement improved kit bodyweight at weaning (934 vs 1104 g; P < 0.001), and average daily weight gain from birth to weaning (24.7 vs 29.7 g/day; P < 0.001), with no NE dose effect. In the LNE and HNE groups serum triglycerides, total cholesterol, low density lipoprotein cholesterol, bilirubin, and activities of alkaline phosphatase, alanine aminotransferase (P < 0.05) and aspartate aminotransferase (P < 0.01) decreased and high density lipoprotein cholesterol (P < 0.01) increased, according to the cycle effect. The dietary supplement also improved blood oxidative status markers in the experimental groups due to an increase in the concentrations of plasma vitamin A and E (P < 0.01) and a decrease in plasma reactive oxygen metabolites and thiobarbituric acid reactive substances values (P < 0.01). In conclusion, the dietary Lippia NE supplement improved selected productive and reproductive parameters and the animal welfare of does, expressed by a general improvement of blood profile, with no effect of the dose.

scholarly journals  Influence of Lippia citriodora verbascoside on growth performance, antioxidant status, and serum immunoglobulins content in piglets

2012 ◽  
Vol 57 (No. 7) ◽  
pp. 312-322 ◽  
Author(s):  
G. Pastorelli ◽  
R. Rossi ◽  
C. Corino
Keyword(s):  
Growth Performance ◽  
Plant Extract ◽  
Antioxidant Status ◽  
Density Lipoprotein ◽  
Feed Additives ◽  
Lipoprotein Cholesterol ◽  
Feed Consumption ◽  
Reactive Oxygen Metabolites ◽  
Lippia Citriodora ◽  
Over Time

Phytogenic feed additives are used in animal feeding to improve livestock performance. The aim of this study was to determine whether a dietary plant extract from Lippia citriodora, standardized for verbascoside, can modify various immunological, oxidative, and biochemical serum parameters in weaned piglets. A total of 144 piglets, half female and half barrows (7.99 &plusmn; 1.40 kg BW), were allocated to three dietary treatments with six replicates per treatment (pens of 8 piglets each). Piglets were supplemented with the following levels of plant extract standardized for verbascoside: 0 (CON = control group), 5 (LV = low verbascoside group), or 10 (HV = high verbascoside group) mg/kg of diet for 56 days. Body weight (BW) and feed consumption were recorded at days 0 and 56 to determine the average daily gain and gain : feed. Twelve piglets from each treatment were randomly selected, and blood was collected by anterior vena cava puncture on days 0, 14, and 56 for glucose, triglycerides, cholesterol, urea, and oxidative status, and on days 0 and 56 for IgG and IgA concentrations. The HV group grew more (P &lt; 0.05) than the CON and LV groups. No significant differences were observed for any of the biochemical parameters between the groups; urea, high density lipoprotein cholesterol, total cholesterol, and low density lipoprotein cholesterol increased significantly over time. Reactive oxygen metabolites (ROM) showed significant time, time &times; treatment, and treatment effects (P &lt; 0.001). Both serum Igs increased (P &lt; 0.005, P &lt; 0.001 for IgG and IgA respectively) over time in all groups; treatment (P &lt; 0.05), and time &times; treatment (P = 0.056) effects were found for serum IgA concentration. The Lippia citriodora verbascoside positively influenced antioxidant status and IgA content with a tendential effect on growth performance. &nbsp;

scholarly journals Effects of Lippia citriodora Leaf Extract on Lipid and Oxidative Blood Profile of Volunteers with Hypercholesterolemia: A Preliminary Study

Antioxidants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 521
Author(s):  
Antonella Angiolillo ◽  
Deborah Leccese ◽  
Marisa Palazzo ◽  
Francesco Vizzarri ◽  
Donato Casamassima ◽  
...  
Keyword(s):  
Lipid Profile ◽  
Leaf Extract ◽  
Density Lipoprotein ◽  
Lipoprotein Cholesterol ◽  
Plasma Vitamin ◽  
Reactive Oxygen Metabolites ◽  
Blood Profile ◽  
Antioxidant Power ◽  
Lippia Citriodora ◽  
Preliminary Study

Lippia citriodora is a plant traditionally used for its anti-inflammatory, antioxidant and antispasmodic effects, as well as for additional biological activities proven in cell culture, animal studies and a small number of human clinical trials. The plant has also shown a marked improvement in blood lipid profile in some animal species. In the present preliminary study, we investigated the effect of a leaf extract on lipid and oxidative blood profile of hypercholesterolemic volunteers. Twelve adults received Lippia citriodora extract caps, containing 23% phenylpropanoids, (100 mg, once a day) for 16 weeks. Selected blood lipids and plasma oxidative markers were measured at baseline and after 4, 8 and 16 weeks of treatment. Compared with baseline, total cholesterol levels significantly decreased and high-density lipoprotein cholesterol increased, while low-density lipoprotein cholesterol and triglycerides showed only a downward trend. Oxidative status was improved due to a decrease in the concentration of total oxidant status, reactive oxygen metabolites and malondialdehyde, and a significant increase in ferric reducing ability of plasma, vitamin A and vitamin E. These preliminary results suggest that dietary supplementation with Lippia citriodora extract can improve the lipid profile, enhance blood antioxidant power, and could be a valuable natural compound for the management of human hypercholesterolemia.

scholarly journals Supplementation-Dependent Effects of Vegetable Oils with Varying Fatty Acid Compositions on Anthropometric and Biochemical Parameters in Obese Women

Nutrients ◽  
2018 ◽  
Vol 10 (7) ◽  
pp. 932 ◽  
Author(s):  
Luciene Oliveira-de-Lira ◽  
Eduila Santos ◽  
Raphael de Souza ◽  
Rhowena Matos ◽  
Matilde Silva ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Biochemical Parameters ◽  
Density Lipoprotein ◽  
Coconut Oil ◽  
Lipoprotein Cholesterol ◽  
Obese Women ◽  
Lifestyle Modifications ◽  
Anthropometric Parameters ◽  
Chia Oil ◽  
Fatty Acid Compositions

Fatty acid (FA) composition is a determinant of the physiological effects of dietary oils. This study investigated the effects of vegetable oil supplementation with different FA compositions on anthropometric and biochemical parameters in obese women on a hypocaloric diet with lifestyle modifications. Seventy-five women (body mass index, BMI, 30–39.9kg/m2) were randomized based on 8-week oil supplementation into four experimental groups: the coconut oil group (CoG, n = 18), the safflower oil group (SafG, n = 19), the chia oil group (ChG, n = 19), and the soybean oil placebo group (PG, n = 19). Pre- and post-supplementation weight, anthropometric parameters, and body fat (%BF), and lean mass percentages (%LM) were evaluated, along with biochemical parameters related to lipid and glycidemic profiles. In the anthropometric evaluation, the CoG showed greater weight loss (Δ% = −8.54 ± 2.38), and reduced BMI (absolute variation, Δabs = −2.86 ± 0.79), waist circumference (Δabs = −6.61 ± 0.85), waist-to-height ratio (Δabs = −0.041 ± 0.006), conicity index (Δabs = −0.03 ± 0.016), and %BF (Δabs = −2.78 ± 0.46), but increased %LM (Δabs = 2.61 ± 1.40) (p < 0.001). Moreover, the CoG showed a higher reduction in biochemical parameters of glycemia (Δabs = −24.71 ± 8.13) and glycated hemoglobin (Δabs = −0.86 ± 0.28) (p < 0.001). The ChG showed a higher reduction in cholesterol (Δabs = −45.36 ± 0.94), low-density lipoprotein cholesterol (LDLc; Δabs = −42.53 ± 22.65), and triglycerides (Δabs = −49.74 ± 26.3), but an increase in high-density lipoprotein cholesterol (HDLc; abs = 3.73 ± 1.24, p = 0.007). Coconut oil had a more pronounced effect on abdominal adiposity and glycidic profile, whereas chia oil had a higher effect on improving the lipid profile. Indeed, supplementation with different fatty acid compositions resulted in specific responses.

scholarly journals Comparative biochemical studies of aqueous extract of Egyptian black and raw garlic (Allium sativum) on hypercholesterolemia in rats fed a high-fat diet

2020 ◽  
Vol 8 (4) ◽  
pp. 132-138
Author(s):  
Abd El Wahab A. E. Safaa ◽  
◽  
A. M. Abdul Azeem ◽  
Mansour A. Amal ◽  
Al Shammari M. Eida ◽  
...  
Keyword(s):  
High Fat Diet ◽  
Biochemical Parameters ◽  
Allium Sativum ◽  
Density Lipoprotein ◽  
Normal Diet ◽  
Garlic Extract ◽  
Lipoprotein Cholesterol ◽  
Albino Rats ◽  
High Fat ◽  
High Concentration

Black garlic is a type of garlic that is processed for a specific period, with precisely controlled degrees of moisture and heat until the color of raw garlic turns black. Black garlic plays a strong role in preventing diseases such as hyperlipidemia. This study aims to compare the effect of Egyptian black garlic extract (EBG) and Egyptian raw garlic extract (ERG) at different concentrations on serum biochemical parameters in hyperlipidemia rats’ model. Fifty albino rats were divided randomly into 10 groups with five animals. Rats were fed a normal diet (control) G1, high-fat diet (HFD) 40% lard w/w were (G2- G10) for four weeks to promoting hypercholesterolemia for all rats except G1 (control). After that, HFD groups were treated with 1ml oral administration three times a week of 100% (G3), 75% (G4), 50% (G5), and 25% (G6) EBG extract for 6 weeks. G2 (HFD) was a high-fat diet alone without any extract. ERG extract feed group were treated in the same manner described above, and G7 (HFD+100% ERG extract), G8 (HFD+75% ERG extract), G9 (HFD+50% ERG extract), and G10 (HFD+25% ERG extract) as written here. EBG extract-treated group showed significantly increased high-density lipoprotein cholesterol (HDL-C) in serum (p < 0.05), decreased both level of total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and triglycerides (TG) in serum. EBG extract with high concentration (G3) causes down regulation of lipid and cholesterol (p < 0.05) comparing with ERG extract indicating usefulness for treating hypercholesterolemia. Body weights on rats decrease by feeding of EBG extract, along with liver enzymes decrease in EBG fed rats. This indicates the efficiency of black garlic extract in reducing the level of hypercholesterolemia and avoiding heart disease and atherosclerosis. Keywords: Allium sativum, garlic, black garlic, hypercholesterolemia, biochemical parameters.

scholarly journals Evaluation of Antihyperlipidaemic and Antioxidant Activity of Astercantha longifolia (Linn.) Nees and Pergularia daemia (Forsskal) Chiov

2020 ◽  
pp. 134-138
Author(s):  
T. P. Kumari Pushpa Rani ◽  
A. Doss ◽  
S. P. Anand
Keyword(s):  
Antioxidant Activity ◽  
Biochemical Parameters ◽  
Methanol Extract ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Lipoprotein Cholesterol ◽  
Low Density ◽  
Low Density Lipoprotein Cholesterol

Objective: To evaluate the antioxidant and antihyperlipidaemic effect of methanol extracts of Asteracantha longifolia and Pergularia daemia leaf in alloxan induced diabetic rats. Methods: Swiss albino rats were made diabetic by a single dose of alloxan monohydrate (150 mg/kg i.p.). Blood glucose levels and body weights of rats were measured using on weekly intervals i.e day 0,7,14 and 21 after daily administration of both extracts at dose 200 mg/kg bw. Other biochemical parameters such as serum cholesterol, triglycerides, low density lipoprotein-cholesterol, very low density lipoprotein-cholesterol, high density lipoprotein-cholesterol, phospholipids and serum protein, albumin, globulin levels were also measured at the end of study. The antioxidant enzymes (CAT, SOD & GSH) were also measured in the diabetic rats. Results: In the acute toxicity study, methanol extract of both plants were non-toxic at 2000 mg/kg in rats. The increased body weight, decreased blood glucose and other biochemical parameters level were observed in diabetic rats treated with methanol extracts of both plants compared to diabetic control rats. The antioxidant activity of both plant extracts were also exhibited significant activity. In diabetic rats, methanol extract of both plants administration, altered lipid profiles were reversed to near normal than diabetic control rats. Conclusions: Methanol extract of both plants (Asteracantha longifolia and Pergularia daemia) leaf possesses significant antioxidant and antihyperlipidaemic activity in diabetic rats.

scholarly journals Effect of stocking density on behavioral traits, blood biochemical parameters and immune responses in meat ducks exposed to heat stress

2018 ◽  
Vol 61 (4) ◽  
pp. 425-432 ◽  
Author(s):  
Byung-Sung Park ◽  
Kyung-Hwan Um ◽  
Sang-O Park ◽  
Victor A. Zammit
Keyword(s):  
Heat Stress ◽  
Immune Responses ◽  
Biochemical Parameters ◽  
Density Lipoprotein ◽  
Stocking Density ◽  
Stress Conditions ◽  
Lipoprotein Cholesterol ◽  
Blood Biochemical Parameters ◽  
Behavioral Traits ◽  
Blood Biochemical

Abstract. High stocking density (HSD) and heat stress (HS) caused by climate change can lower blood homeostasis and negatively impact the behavioral traits of animals. The objective of this study was to explore the influence of stocking densities on behavioral traits, blood parameters, immune responses, and stress hormones in meat ducks (Cherry valley, Anas platyrhynchos) exposed to HS. A total of 320 meat ducks were assigned to four groups with different stocking densities using a randomized complete block design. The ducks were then reared for 42 days. The assigned density groups were as follows: (1) control group (CON, three birds m−2, normal environmental heat conditions); (2) low stocking density (LSD, three birds m−2, heat stress conditions); (3) medium stocking density (MSD, four birds m−2, heat stress conditions); and (4) high stocking density (HSD, six birds m−2, heat stress conditions). To induce HS, the environment of the poultry house was set to a temperature of 34±2∘C with a relative humidity of 70 % from 11:00 to 16:00 for the finisher period (from day 22 to day 42 of the rearing period). Concentrations of blood triacylglycerol, total cholesterol, low-density lipoprotein cholesterol (LDL-C), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) were higher in the HS groups compared with the CON group, with HSD showing the highest levels (P < 0.05). The concentrations of high-density lipoprotein cholesterol (HDL-C) and glucose were lower in the HSD groups than in the CON group (P<0.05). Red blood cell (RBC) and platelet (PLT) counts were lower in HS groups compared with the CON group, with the HSD group displaying the lowest counts (P<0.05). Blood pH values were also higher in the HS groups than in the CON group, with the highest values observed in the HSD group (P<0.05). Concentrations of blood pCO2, HCO3, and TCO2 were higher in the HS groups than in the CON group, with HSD showing the lowest levels (P<0.05). The concentration of PO2 was higher in CON than in any of the HS groups, with the lowest levels found in the HSD group (P<0.05). The concentrations of blood IgG and corticosterone were increased in the HS groups compared with the CON group (P<0.05). Animal behavioral trait scores were also higher in HS groups than in the CON group (P<0.05); these scores were the highest in the HSD group. Overall, animal behavioral traits, blood biochemical parameters, and immune responses in meat ducks exposed to heat stress were highest in the HSD group, but not significantly different between the LSD or MSD groups.

249 BIOPHYSICAL AND BIOCHEMICAL CHARACTERISTICS OF ALPACA SEMEN AFTER COLLECTION BY ELECTROEJACULATION

2013 ◽  
Vol 25 (1) ◽  
pp. 272 ◽  
Author(s):  
J. Rodriguez ◽  
W. Huanca ◽  
M. Ramos ◽  
M. Vasquez ◽  
J. Espinoza
Keyword(s):  
Alkaline Phosphatase ◽  
High Density Lipoprotein ◽  
High Density Lipoprotein Cholesterol ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Density Lipoprotein ◽  
Alanine Transaminase ◽  
High Density ◽  
Lipoprotein Cholesterol ◽  
Biochemical Characteristics

The collection of alpaca semen presents difficulties because of the characteristics of mounts and copulation time in addition to the high viscosity of semen. The biophysical and biochemical characteristics of alpaca semen were investigated. Semen samples from four adult males from 6 to 7 years of age were collected using an electroejaculation technique. The biophysical parameters measured in seminal plasma included volume, color, sperm concentration, motility, and viscosity, and the biochemical parameters included pH and glucose, calcium, triglyceride, cholesterol, high-density lipoprotein cholesterol, total protein, albumin, alanine transaminase, alkaline phosphatase, and γ-glutamyltranspeptidase concentrations. The mean time for semen collection was 10.3 ± 1.5 min; the volume varied from 1.2 to 3.8 and the colour from clear to milky, and pH was 7.4 ± 0.03. Mean concentration of spermatozoa was 69.3 ± 193 million mL–1, and motility was 52.4 ± 9.7%. Biochemical parameters were glucose (mg dL–1) 8.22 ± 0.77; cholesterol (mg dL–1) 79.78 ± 5.64; triglycerides (mg dL–1) 44.12 ± 7.38; high-density lipoprotein cholesterol (mg dL–1) 4.73 ± 0.30; total protein (g dL–1) 2.36 ± 0.15; albumin (g dL–1) 0.97 ± 0.33; calcium (mg dL–1) 11.77 ± 1.74; alanine transaminase (U L–1) 17.92 ± 9.09; alkaline phosphatase (U L–1) 288.76 ± 279.59; and γ-glutamyltranspeptidase (U L–1) 89.62 ± 39.09. These results provide information on the biophysical and biochemical characteristics of alpaca semen collected by electroejaculation, with variable results in the enzymatic components.

scholarly journals Study of LCAT and some biochemical parameters in cardiovascular patient

2020 ◽  
Vol 25 (2) ◽  
pp. 25
Author(s):  
Amel Taha Yassein Al-Juraisy1 ◽  
Ameera Aziz Mahmood Al-Juraisy1 ◽  
Nadia Ahmed Saleh2
Keyword(s):  
Biochemical Parameters ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Lipoprotein Cholesterol ◽  
Apolipoprotein Ai ◽  
Low Density Lipoprotein Cholesterol ◽  
Cholesterol Acyl Transferase ◽  
Lecithin Cholesterol Acyl Transferase ◽  
Cardiovascular Patient ◽  
Apolipoprotein B100

The Clinical study included individuals (m23ales and females aged 25-75y) with cardiovascular disease. The activity of Lecithin: cholesterol acyl transferase (LCAT) and glutathione peroxidase(GPx) was estimated, in addition to the level of apolipoprotein AI (apo AI), apolipoprotein B100 (apoB100), total cholesterol (TC), triglyceride (TG), high density lipoprotein-cholesterol (HDL-c), low density lipoprotein-cholesterol (LDL-c), glutathione (GSH), peroxy nitrate (PN), malondialdehyde (MDA), glucose and certain elements (Zn, Cu, Mg and Co) in blood serum. The results showed that there was a significant decrease in the activity of LCAT and GPx, and the level of apoAI, HDL-c, GSH, Zn and Mg. on the other hand, it was shown that there was a significant increase in the level of apoB100, TC, TG,  LDL-c, PN, Glu, Cu and Co but on unsignificant change in the level of MDA for both male and females.   http://dx.doi.org/10.25130/tjps.25.2020.026

scholarly journals Determination of Some Biochemical Parameters in Streptozotocin-Induced Diabetic Albino Rats Pre-Treated and Post-Treated with Vernonia amygdalina and Gongronema latifolium Extracts

2019 ◽  
pp. 1-12
Author(s):  
Ibifiri Bristol ◽  
Ebirien-Agana Samuel Bartimaeus ◽  
Holy Brown
Keyword(s):  
Diabetes Mellitus ◽  
Biochemical Parameters ◽  
Density Lipoprotein ◽  
Treatment Phase ◽  
Lipoprotein Cholesterol ◽  
Atherogenic Index ◽  
Albino Rats ◽  
Vernonia Amygdalina ◽  
Atherogenic Index Of Plasma ◽  
Gongronema Latifolium

The use of herbs in the management of diabetes mellitus and its complications have been reported. This study was thus aimed at determining the levels of some biochemical parameters in streptozotocin-induced diabetic albino rats pre-treated and post-treated with Vernonia amygdalina and Gongronema latifolium extracts. Fifty (50) albino rats weighing between 150 – 250 g were used for this study. 25 albino rats were used for each phase of the treatment. The pre-treatment phase involved the treatment of the rats with 400 mg/kg b.w (singly) and 200 mg/kg b.w (combined) extracts for 14 days, after which diabetes mellitus was induced using streptozotocin before the rats were sacrificed. The post-treatment phase involved the inducement of diabetes with streptozotocin after which the rats were treated with 400 mg/kg b.w (singly) and 200 mg/kg b.w (combined) extracts for 28 days before the animals were sacrificed. Blood was collected via cardiac puncture and plasma/serum was collected. Total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), and total antioxidant capacity (TAC) were determined using standard procedures while glucose was measured with a glucometer. Cardiovascular risk ratios such as Castelli risk ratio I and II and atherogenic index of plasma were also calculated. Results showed that extracts of V. amygdalina (only), G. latifolium (only) and V.A+ GL (combined)significantly (p<0.05) reduced the TC, LDL-C , FBS, MDA and TAC levels while HDL-C level significantly (p<0.05) increased. The cardiac risk indices (CRI-I and CRI-II, and Atherogenic index of plasma (AIP) in both the pre-treated and post-treated rats were also reduced when compared to diabetic control. It is thus evident that these plants’ extracts possess hypoglycaemic, hypolipidaemic, and antioxidant properties and thus could be used to reduce cardiovascular risks. Therefore, the tradomedicinal use of these plants in the management of cardiovascular complications is highly recommended.

scholarly journals Assessment of Some Biochemical Parameters in Vitreous Humor of Rabbits Exposed to Sodium Cyanide

2021 ◽  
pp. 41-47
Author(s):  
B. I. Elleh ◽  
E. S. Bartimaeus ◽  
F. U. Igwe ◽  
D. G. Tamuno-Emine ◽  
E. S. Agoro
Keyword(s):  
Biochemical Parameters ◽  
Standard Procedure ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Vitreous Humor ◽  
Sodium Cyanide ◽  
Lipoprotein Cholesterol ◽  
Low Density Lipoprotein Cholesterol ◽  
Cyanide Poisoning ◽  
Rivers State

Aim: The aim of this study was to assess some biochemical parameters in the vitreous humor of Rabbits exposed to sodium cyanide. Study Design: This study is an interventional study. Place and Duration of Study: This study was carried out at Animal House, Applied and Environmental Biology Department, Rivers State University, Port Harcourt, Rivers State, Nigeria, between April 2020 and November 2020. Methodology: A total of twelve (12) rabbits as indicated by Mead’s formula constituted the sample size. The study was divided into three groups including the control. 1 mg/kg sodium cyanide was administered to the rabbits orally in group one and vitreous humor was collected using standard procedure after thirty minutes. In group two the rabbits were put to death mechanically and 1 mg/kg sodium cyanide was administered to the rabbits after thirty minutes, vitreous humor was collected after thirty minutes using standard procedure. The rabbits in group three, control group, were put to death mechanically and nothing was given to the rabbits, then after thirty minutes vitreous humor was collected using standard procedure. Biochemical parameters investigated included vitreous glucose, total cholesterol, triglycerides, high density lipoprotein cholesterol, low density lipoprotein cholesterol, very low density lipoprotein cholesterol, total proteins (TP), albumin (ALB), total bilirubin, conjugated bilirubin, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities. Data were expressed as mean ± SD. Statistical differences between groups were computed using Graph pad prism 7.0 version developed by Graph pad software, San Siago, Califonia, USA. Results were analyzed using analysis of variance (ANOVA) and significance between groups was taken at p<.05. Results: Vitreous biochemical results showed significant (p<.05) increase in levels of TC, LDL-C, AST, ALT, TB and CB in rabbits given 1 mg/kg sodium cyanide compared to control. Significant (p<.05) decrease in levels of glucose, TP and ALB were also observed. This study also revealed that there was no significant (p<.05) difference in biochemical results of rabbits given 1 mg/kg sodium cyanide thirty minutes after they were put to death mechanically compared to the control. It can be concluded that a lethal dose of sodium cyanide (1 mg/kg) may lead to alterations in vitreous biochemical parameters and this may enhance death differentiation due to sodium cyanide poisoning and other causes of death for example mechanical death.    Conclusion: The findings of this study support a central role for vitreous humor biochemistry in many postmortem forensic and pathological evaluations and it could also be used for death differentiation in sodium cyanide poisoning.

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