scholarly journals Intake, apparent digestibility, and methane emission in bulls receiving a feed supplement of monensin, virginiamycin, or a combination

2016 ◽  
Vol 56 (7) ◽  
pp. 1041 ◽  
Author(s):  
Marcelina Pereira da Fonseca ◽  
Ana Luiza da Costa Cruz Borges ◽  
Ricardo Reis e Silva ◽  
Helena Ferreira Lage ◽  
Alexandre Lima Ferreira ◽  
...  

The aim of this study was to evaluate the effects of the feed supplements monensin, virginiamycin, or a combination of the two, on intake, digestibility, and methane emission in the male cattle breed F1 Holstein × Gir. We used a complete randomised design with four treatments consisting of the control, monensin, virginiamycin, and a combination of the two. The basal diets were composed of sorghum silage with Tanzania grass and the concentrate in the 1 : 1 ratio. Nutrient intake (P > 0.05) and the apparent digestibility coefficients (P > 0.05) were not affected by the supplementation with monensin, virginiamycin, or both. The combination of the supplements did affect methane emission (P < 0.05) when expressed in L/day, L/(kg DM), and L/(kg DM digestible). The lowest methane production was obtained with the combination of the supplements.

2007 ◽  
Vol 135 (3-4) ◽  
pp. 236-248 ◽  
Author(s):  
Simone Gisele de Oliveira ◽  
Telma Teresinha Berchielli ◽  
Márcio dos Santos Pedreira ◽  
Odo Primavesi ◽  
Rosa Frighetto ◽  
...  

2016 ◽  
Vol 7 (1) ◽  
pp. 1
Author(s):  
Susi Dwi Widyawati

<p><em>Th</em><em>e</em><em> research </em><em>wa</em><em>s done</em><em> to study the potential of ketepeng leaf meal and lemuru fish oil as agents to reduce methane production</em><em>. The composition of feed supplements, lamtoro leave meal (LLM), urea (U) and mollases (M) </em><em>were </em><em>in different ratios</em><em>:</em><em> 1:5, 1:6 and 1:7 and ketepeng</em><em> leaf</em><em> meal (DKM) and lemuru fish oil (MIL). The feed supplement was added to the native grass as a basal diet</em><em>,</em><em> P1: U: M = 1:5, DKM, P2: U: M = 1:5, MIL, P3: U: M = 1:6, DKM, P4: U: M = 1:6, MIL, P5: U: M = 1:7, DKM, P6: U: M = 1:7, MIL. </em><em>R</em><em>esearch </em><em>was </em><em>done with in vitro gas technique. </em><em>I</em><em>noculum </em><em>was taken from </em><em>rumen fluid </em><em>of</em><em> a </em><em>fistulated </em><em>male cow</em><em>,</em><em> </em><em>which was taken</em><em> before feed</em><em>ing</em><em> in the morning. </em><em>R</em><em>umen fluid pH </em><em>did not </em><em>significant</em><em>ly</em><em> different, </em><em>indicating</em><em> the microbial fermentation in rumen run well and normally </em><em>we</em><em>re not affected by the treatment. </em><em>The VFA</em><em> production</em><em> of</em><em> P5 and P6</em><em> was significantly lower than</em><em> P1, P2, P3 and P4 </em><em>(</em><em>P &lt;0.05). Production of C2 and C3</em><em> were</em><em> relatively the same except </em><em>for</em><em> P6 (P &lt;0.05) </em><em>which relative higher</em><em>. </em><em>Ratio of</em><em> C2/C3 and inversely related C3, </em><em>when </em><em>C3 production </em><em>increased the </em><em>C2/C3 ratio </em><em>decreased </em><em>(P &lt;0.05)</em><em>. M</em><em>ethane production </em><em>of</em><em> P6 </em><em>was higher but did not</em><em> different </em><em>with</em><em> P4 and P5. </em><em>It can be concluded that</em><em> diet </em><em>which </em><em>produced low methane </em><em>was</em><em> the native grass supplemented with urea and molases at ratio 1: 5 </em><em>contained either </em><em>ketepeng lea</em><em>f</em><em> meal </em><em>or</em><em> lemuru fish oil. </em><em></em></p><p><em><br /> <strong>Key</strong></em><strong><em> </em></strong><strong><em>words</em></strong><em>: native grass, supplement, ketepeng leaf meal, lemuru fish oil, methane  production</em><em>.</em></p>


2016 ◽  
Vol 7 (1) ◽  
pp. 1
Author(s):  
Susi Dwi Widyawati

<p><em>Th</em><em>e</em><em> research </em><em>wa</em><em>s done</em><em> to study the potential of ketepeng leaf meal and lemuru fish oil as agents to reduce methane production</em><em>. The composition of feed supplements, lamtoro leave meal (LLM), urea (U) and mollases (M) </em><em>were </em><em>in different ratios</em><em>:</em><em> 1:5, 1:6 and 1:7 and ketepeng</em><em> leaf</em><em> meal (DKM) and lemuru fish oil (MIL). The feed supplement was added to the native grass as a basal diet</em><em>,</em><em> P1: U: M = 1:5, DKM, P2: U: M = 1:5, MIL, P3: U: M = 1:6, DKM, P4: U: M = 1:6, MIL, P5: U: M = 1:7, DKM, P6: U: M = 1:7, MIL. </em><em>R</em><em>esearch </em><em>was </em><em>done with in vitro gas technique. </em><em>I</em><em>noculum </em><em>was taken from </em><em>rumen fluid </em><em>of</em><em> a </em><em>fistulated </em><em>male cow</em><em>,</em><em> </em><em>which was taken</em><em> before feed</em><em>ing</em><em> in the morning. </em><em>R</em><em>umen fluid pH </em><em>did not </em><em>significant</em><em>ly</em><em> different, </em><em>indicating</em><em> the microbial fermentation in rumen run well and normally </em><em>we</em><em>re not affected by the treatment. </em><em>The VFA</em><em> production</em><em> of</em><em> P5 and P6</em><em> was significantly lower than</em><em> P1, P2, P3 and P4 </em><em>(</em><em>P &lt;0.05). Production of C2 and C3</em><em> were</em><em> relatively the same except </em><em>for</em><em> P6 (P &lt;0.05) </em><em>which relative higher</em><em>. </em><em>Ratio of</em><em> C2/C3 and inversely related C3, </em><em>when </em><em>C3 production </em><em>increased the </em><em>C2/C3 ratio </em><em>decreased </em><em>(P &lt;0.05)</em><em>. M</em><em>ethane production </em><em>of</em><em> P6 </em><em>was higher but did not</em><em> different </em><em>with</em><em> P4 and P5. </em><em>It can be concluded that</em><em> diet </em><em>which </em><em>produced low methane </em><em>was</em><em> the native grass supplemented with urea and molases at ratio 1: 5 </em><em>contained either </em><em>ketepeng lea</em><em>f</em><em> meal </em><em>or</em><em> lemuru fish oil. </em><em></em></p><p><em><br /> <strong>Key</strong></em><strong><em> </em></strong><strong><em>words</em></strong><em>: native grass, supplement, ketepeng leaf meal, lemuru fish oil, methane  production</em><em>.</em></p>


1977 ◽  
Vol 60 (5) ◽  
pp. 1059-1063
Author(s):  
John T Goras

Abstract A colorimetric method for determining carbadox in complete swine feeds and feed supplements was collaboratively studied. Carbadox is separated from feed with CHCl3-methanol (3+1) and then separated from interfering materials by a series of solvent-solvent extractions. The drug is isolated as a dry residue, reconstituted, and reacted with stannous chloride to form a colored complex that is measured at 520 nm. The method of standard additions is used to compensate for a feed or feed supplement matrix effect. Twenty-seven laboratories assayed feeds containing 0.0013, 0.0053, and 0.0242% carbadox. The repeatability standard deviation (σ0) and reproducibility standard deviation (σx) were σ0 = 0.00014%, σx = 0.00035% (29% of grand mean) for 0.0013% carbadox in feed; σ0 = 0.00025%, σx = 0.00037% (6.7% of grand mean) for 0.0053% carbadox in feed; and σ0 = 0.0019%, σx = 0.0024% (9.6% of grand mean) for 0.0242% carbadox in feed. The between-laboratory variance ratio was not significant for feeds containing 0.0013 and 0.0053% carbadox, but was significant for feeds containing 0.0242% carbadox. The mean recovery values for feeds containing 0.0013, 0.0053, and 0.0242% carbadox were 92, 104, and 103%, respectively. The method was adopted as official first action for feeds having a guaranteed potency of 0.0055% carbadox or higher.


2017 ◽  
Vol 46 (2) ◽  
pp. 150-158
Author(s):  
SMR Rahman ◽  
NR Sarker ◽  
MR Amin ◽  
M Kamruzzaman ◽  
MR Haque

An investigation was carried out with the objectives to identify the naturally occurring forage species, seasonal availability, production patterns under different climatic zones and production performance and methane emission from dairy cow under existing feeding systems. For this purpose, three different agro-climatic zones of Bangladesh, namely saline prone area (Satkhira), flood plain/river basin areas (Pabna), semi-arid/drought prone areas (Chapainobabgonj) were selected. To achieve the objectives, three Focus Group Discussions (FGD) were conducted in each location to obtain more information from different age groups of farmers. A total of 9 FDGs were conducted under three selected locations and twelve participants were attended in each FGD. During FDGs, information was collected through participatory discussions through check list and also discussion was recorded to verify the information gathered as per check list.  After collection of information in each side, all the data were checked and analyzed. The results indicated that in saline area, farmers reported that different types of local grass e.g. Tale Shapna,Durba,Nona Shapna, Khud Gate/ KhudKhachra, Shama, Full Paira, Bass Pata, Math Pora/KhataShak, GhimeeShak and Baksha etc were available round the year but according to their observation Nona Shapna, Tale Shapna and Baksha were more available compared to other species of the natural grasses and these three natural forages are more suitable in this area. In the drought prone area, different types of native grasses e.g. Durba,Shama, Mutha,Katla,Kausha/Kannar, Binna, Datuloka,Shanchi, Shunshue, Bash Batari, Ulo and Binna Pati were identified and utilized by the farmers in different seasons but Durba,Katla and Mutha were found more drought tolerant compared to other species. In flood prone area, Kolmi, Shanti, Baksha, Arail, Dubla, Bokma, Vadail and Bolenga etc were found and Kolmi, Baksha and Arail are more suitable in this area. Farmers were also reported that fodder tree like Dumur/khoksha also is survive in water logging situation and or flood prone area. The study revealed that calculated total DMI (Kg/h/day) was the highest (14.14±1.06) in flood prone  followed by drought (13.80±1.30) and saline areas (4.43±0.20),  respectively. Similarly, the milk production was also higher (12.06±1.19 litre/h/day) in flood prone area followed by drought (4.47±0.60 litre/h/day) and saline (1.83±0.11 litre/h/day) areas, respectively. The calculated total methane emission (g/h/d) was significantly higher in flood prone (478.31±36.36) and the lowest in saline (153.35±7.14) prone areas. Whereas, methane production per unit of milk yield, was the lowest in flood prone (46.55±6.78) and the highest (110.48±21.69) in drought prone area and the difference was statistically significant (p<0.05).  Therefore, it may be concluded that farmers’ rearing animals under climate vulnerable areas utilizing natural grasses are more prone to higher methane production compared to animals rearing better feed resources though their availability was varied with the seasons and locations.  Hence, further research is needed to explore more suitable natural grasses in addition to introduction of high yielding fodder with higher biomass and nutritive values based on the existing cropping systems in those climate vulnerable areas for higher milk production and low enteric methane emission in the country.Bang. J. Anim. Sci. 2017. 46 (2): 150-158


2009 ◽  
Vol 6 (6) ◽  
pp. 1127-1138 ◽  
Author(s):  
V. Jerman ◽  
M. Metje ◽  
I. Mandić-Mulec ◽  
P. Frenzel

Abstract. Ljubljana marsh in Slovenia is a 16 000 ha area of partly drained fen, intended to be flooded to restore its ecological functions. The resultant water-logging may create anoxic conditions, eventually stimulating production and emission of methane, the most important greenhouse gas next to carbon dioxide. We examined the upper layer (~30 cm) of Ljubljana marsh soil for microbial processes that would predominate in water-saturated conditions, focusing on the potential for iron reduction, carbon mineralization (CO2 and CH4 production), and methane emission. Methane emission from water-saturated microcosms was near minimum detectable levels even after extended periods of flooding (>5 months). Methane production in anoxic soil slurries started only after a lag period of 84 d at 15°C and a minimum of 7 d at 37°C, the optimum temperature for methanogenesis. This lag was inversely related to iron reduction, which suggested that iron reduction out-competed methanogenesis for electron donors, such as H2 and acetate. Methane production was observed only in samples incubated at 14–38°C. At the beginning of methanogenesis, acetoclastic methanogenesis dominated. In accordance with the preferred substrate, most (91%) mcrA (encoding the methyl coenzyme-M reductase, a key gene in methanogenesis) clone sequences could be affiliated to the acetoclastic genus Methanosarcina. No methanogens were detected in the original soil. However, a diverse community of iron-reducing Geobacteraceae was found. Our results suggest that methane emission can remain transient and low if water-table fluctuations allow re-oxidation of ferrous iron, sustaining iron reduction as the most important process in terminal carbon mineralization.


2020 ◽  
Vol 65 (12) ◽  
pp. 2853-2865 ◽  
Author(s):  
Marco Günthel ◽  
Isabell Klawonn ◽  
Jason Woodhouse ◽  
Mina Bižić ◽  
Danny Ionescu ◽  
...  

1974 ◽  
Vol 57 (4) ◽  
pp. 982-986
Author(s):  
John T Goras ◽  
Donald A Gonci ◽  
Kotaro Murai ◽  
James E Curley ◽  
Philip N Gordon

Abstract The assay method is applicable to samples containing 0.00110-0.0606% carbadox (methyl 3-(2-quinoxalinylmethylene)carbazate - N1, N4-dioxide) (10–550 g/ton). Carbadox is leached from the sample with chloroform-methanol (3+1), followed by a series of solvent-solvent extractions, column chromatography, and finally the spectrophotometric measurement of the carbadox content of the final solution at 420 nm. This treatment of the feed or feed supplement sample serves to isolate the carbadox from materials that might interfere in the spectrophotometric measurement. The method of standard addition compensates for a feed or feed supplement matrix effect in the assay. A water pretreatment step improves recovery of drug from pelleted feeds. Assay results for feeds and feed supplements that were prepared under carefully controlled conditions showed good agreement between the amounts of carbadox added and found. Multiple assay values for feeds containing 0.00551% carbadox exhibited a coefficient of variation of about 5%. Assay results for commercial feed and feed supplement samples indicated that the assay method is applicable to a wide variety of feeds and feed supplements.


2017 ◽  
Vol 48 (2) ◽  
pp. 63-69
Author(s):  
M. Joch ◽  
V. Kudrna ◽  
B. Hučko

AbstractThe objective of this study was to determine the effects of geraniol and camphene at three dosages (300, 600, and 900 mg l-1) on rumen microbial fermentation and methane emission in in vitro batch culture of rumen fluid supplied with a 60 : 40 forage : concentrate substrate (16.2% crude protein, 33.1% neutral detergent fibre). The ionophore antibiotic monensin (8 mg/l) was used as positive control. Compared to control, geraniol significantly (P < 0.05) reduced methane production with increasing doses, with reductions by 10.2, 66.9, and 97.9%. However, total volatile fatty acids (VFA) production and in vitro dry matter digestibility were also reduced (P < 0.05) by all doses of geraniol. Camphene demonstrated weak and unpromising effects on rumen fermentation. Camphene did not decrease (P > 0.05) methane production and slightly decreased (P < 0.05) VFA production. Due to the strong antimethanogenic effect of geraniol a careful selection of dose and combination with other antimethanogenic compounds may be effective in mitigating methane emission from ruminants. However, if a reduction in total VFA production and dry matter digestibility persisted in vivo, geraniol would have a negative effect on animal productivity.


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