scholarly journals Nutritive value for ruminants of two herbaceous South American native legumes: Adesmia bicolor and Adesmia macrostachya

2014 ◽  
Vol 54 (2) ◽  
pp. 158 ◽  
Author(s):  
D. G. Vileta ◽  
M. Grosso ◽  
M. Fondevila
Keyword(s):  
Dry Matter ◽  
Nutritive Value ◽  
Gas Production ◽  
Maturity Stage ◽  
South American ◽  
Neutral Detergent Fibre ◽  
Acid Detergent Fibre ◽  
Fermentation Parameters ◽  
Production Coefficient

Chemical composition, in vitro fermentation and in situ degradability of the native South American legumes Adesmia bicolor and A. macrostachya were studied at vegetative (VG), flowering (FW) and seeding (SD) stages, and compared with those of alfalfa at 10% flowering (AL) in two separate studies. In A. bicolor, crude protein (CP) content was highest for FW, and FW and SD showed higher values than did AL (P < 0.05). At all stages, A. bicolor showed a lower content of neutral detergent fibre and acid detergent fibre than did AL (P < 0.05). The maximum gas production (Coefficient b) was highest for FW, and no differences were recorded between SD and AL, the lowest value being for VG (P < 0.05). The effective dry matter degradability was higher for FW and VG than for SD and AL (P < 0.05), whereas effective CP degradability tended (P = 0.077) to be higher for FW than for the other stages of A. bicolor, but no differences between FW and AL were detected. A. macrostachya had lower CP content (P < 0.001), but its VG and FW stages also had lower proportions of neutral detergent fibre, acid detergent fibre and acid detergent lignin (P < 0.01) than did AL. Chemical and fermentation parameters were affected by maturity of A. macrostachya. The effective dry matter degradability was higher for VG and FW than for SD and AL (P < 0.001), but no differences were apparent on the effective CP degradability. A. bicolor showed a high nutritive value, which was even higher than that of AL, and the maturity stage of this species did not markedly affect its nutritive value, whereas the nutritive value of A. macrostachya reduced as it matured.

Palatability,in situandin vitronutritive value of dried sainfoin (Onobrychis viciifolia)

2010 ◽  
Vol 148 (6) ◽  
pp. 723-733 ◽  
Author(s):  
H. KHALILVANDI-BEHROOZYAR ◽  
M. DEHGHAN-BANADAKY ◽  
K. REZAYAZDI
Keyword(s):  
Organic Matter ◽  
Dry Matter ◽  
Nutritive Value ◽  
Nutrient Content ◽  
Gas Production ◽  
Metabolizable Energy ◽  
Acid Detergent Fibre

SUMMARYThe current study was conducted to determine chemical composition, nutrient content and availability, metabolizable energy (ME) content and nutritive value of sainfoin hay for ruminants. Three ruminally cannulated Holstein cows were used forin situandin vivoexperiments, to determine rumen degradability and digestibility of sainfoin hay. Apparent total tract digestibility of nutrients was determined with feeding of sainfoin hay as the sole diet to achieve 10% more than maintenance energy requirements. Six Zandi ewes were used in the palatability experiment. Means for dry matter (DM), organic matter (OM), crude protein (CP), neutral detergent fibre (NDF), acid detergent fibre (ADF) and condensed tannins (CTs) of sainfoin hay were: 940·4 g/kg and 93·43, 12·13, 47·87, 43·33 and 2·13 g/kg DM, respectively.In situeffective degradability of CP and DM were 0·38 and 0·54 g/g with a ruminal outflow rate of 0·05/h, respectively. OM apparent digestibility was in the range of 0·592–0·689, respectively, for Tilley & Terry and total faecal collection assays. ME content of sainfoin hay, according to different methods (gas production,in vitroandin vivodetermined digestible organic matter in dry matter (DOMD)) was in the range 6·87–10·11 MJ/kg DM. Metabolizable protein (MP) content was 483·4 g/kg CP. Sainfoin was more palatable than alfalfa for sheep. It was concluded that sainfoin has a potential use in ruminant rations, especially if environmental conditions are not suitable for alfalfa production.

scholarly journals Effects of Hybrid and Grain Maturity Stage on the Ruminal Degradation and the Nutritive Value of Maize Forage for Silage

Agriculture ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 251
Author(s):  
Egon Henrique Horst ◽  
Secundino López ◽  
Mikael Neumann ◽  
Francisco Javier Giráldez ◽  
Valter Harry Bumbieris Junior
Keyword(s):  
Dry Matter ◽  
Nutritive Value ◽  
Degradation Kinetics ◽  
Gas Production ◽  
Maturity Stage ◽  
Ruminal Fermentation ◽  
Maturity Stages ◽  
Whole Plant ◽  
Ruminal Degradation

The study aimed to examine the effects of harvesting three maize hybrids at different maturity stages on the ruminal fermentation kinetics, fermentation end-products (volatile fatty acid, ammonia and methane) output, and digestibility of maize plant fractions, as well as the degradability of the resulting silage. Three hybrids were compared (Maximus VIP3, Defender VIP and Feroz VIP) harvested at three grain maturity stages (milk (R3), dough (R4) and dent (R5) grain), while silage samples were collected only at the dent grain stage (R5). Grain digestibility tended to decrease as the maturity stage progressed (p < 0.05), while the whole-plant digestibility increased with increasing maturity for the three evaluated hybrids (p < 0.05). The gas production of whole-plant at 24 h of incubation was higher for the Maximus hybrid than for the others (p < 0.05), with average values of 188, 196 and 207 mL g−1 dry matter at stages R3, R4 and R5, respectively. For the in situ ruminal degradation kinetics of silage, instantly degradable dry matter and fiber potential degradability were greater with Maximus maize forage than with the other two hybrids. From the perspective of in vitro results, the Maximus VIP3 hybrid seems to be the most suitable for silage production when harvested between the dough and the dent grain stage of maturity.

scholarly journals In vitro and in sacco digestibility of almond hulls

2021 ◽  
Vol 159 (7-8) ◽  
pp. 615-621
Author(s):  
K. L. Swanson ◽  
H. M. Bill ◽  
J. Asmus ◽  
J. M. Heguy ◽  
J. G. Fadel ◽  
...  
Keyword(s):  
Dry Matter ◽  
Nutritional Value ◽  
Nutritive Value ◽  
Gas Production ◽  
Metabolizable Energy ◽  
The Other ◽  
Neutral Detergent Fibre ◽  
True Digestibility ◽  
In Sacco

AbstractThe aim of this study was to determine the in vitro digestibility and in sacco disappearance of dry matter (DM) and neutral detergent fibre (NDF) in total almond hulls (TAH), pure almond hulls (PAH) or Debris. The TAH were used because there are no data on the effect of debris (non-hull material) on the nutritional value of almond hulls. Twelve samples of commercial almond hulls were used, with one subsample representing the TAH and the other subsample hand sorted to separate the hulls (PAH) from Debris. Gas production and Ankom Daisy method were used to determine in vitro digestibility, while two rumen-fistulated cows were used to measure in sacco disappearance of PAH and TAH. For in vitro digestibility, both PAH and TAH were more digestible and had greater gas production than Debris. The PAH had greater in vitro true digestibility on a DM basis and NDF digestibility at 48 and 72 h compared with TAH. Nonpareil hulls provided greater metabolizable energy (ME) concentration when compared with other almond varieties, with PAH supplying numerically more ME than TAH for both varieties. For in sacco disappearance, PAH had greater DM and NDF disappearance along with a greater rate of disappearance for NDF compared with TAH. This research demonstrated that Debris is highly indigestible; therefore, hulling, agronomic and harvesting practices should be focused on reducing Debris in commercial almond hulls to improve their nutritive value as a feedstuff for livestock.

Condensed tannins extracted from Quebracho affect the fermentation profile, nutritional quality and in vitro gas production of pornunça (Manihot spp.) silages

2021 ◽  
pp. 1-10
Author(s):  
D. T. Q. Carvalho ◽  
A. R. F. Lucena ◽  
T. V. C. Nascimento ◽  
L. M. L. Moura ◽  
P. D. R. Marcelino ◽  
...  
Keyword(s):  
Nutritional Quality ◽  
Dry Matter ◽  
Condensed Tannin ◽  
Gas Production ◽  
Rapid Decline ◽  
Randomized Design ◽  
In Vitro Gas Production ◽  
Acid Detergent Fibre ◽  
Fermentation Profile

Abstract The objective was to evaluate the fermentation profile, in vitro gas production and nutritional quality of pornunça (Manihot spp.) silages containing levels of condensed tannin (CT; 0, 4, 8 and 12% on dry matter (DM) basis), at five opening times (0, 3, 7, 14, 28 and 56 days). A completely randomized design in a 4 × 5 factorial arrangement was adopted, with four replications, totalling 80 experimental silos. The pH and NH3-N analyses were performed at all opening times of the silos. The other analyses were performed only with silages opened at 56 days of storage. There was an interaction effect between CT levels and silo opening times for pH and NH3-N. Tannin levels in pornunça silages after 56 days ensiling increased the pH and DM and reduced crude protein (CP) and neutral detergent fibre (NDF). There was a quadratic effect for NH3-N, acetic acid, butyric acid, gas losses, dry matter recovery (DMR), hemicellulose and acid detergent fibre. Inclusion of 4 and 8% CT in pornunça silage promotes a rapid decline in pH, being within the acceptable limit for adequate fermentation at 3 days of ensiling. Silages with 4% CT establish the pH at 28 days of opening the silos, with reduced NH3-N. Silages with 4% CT present higher concentrations of acetic and butyric acids and greater DMR. Inclusion of CT in pornunça silage after 56 days ensiling increases DM and reduces CP and NDF, directly affecting the in vitro degradability and reducing gas production.

scholarly journals PSVI-42 Effect of increasing concentrations of aflatoxins (AFB1) on rumen gas production (GP), dry matter (DM) disappearance and fermentation parameters using the in vitro ANKOM gas technique

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 194-195
Author(s):  
Jean-Philippe Marden ◽  
Virginie Marquis ◽  
Kheira Hadjeba Medjdoub ◽  
Marine Lacombe
Keyword(s):  
Feed Efficiency ◽  
Dry Matter ◽  
In Vitro Model ◽  
Rumen Fluid ◽  
Gas Production ◽  
Corn Silage ◽  
Vitro Model ◽  
Fermentation Parameters ◽  
Cheese Cloth

Abstract Aflatoxins are secondary metabolites produced by Aspergillus species known to be the most prevalent contaminants in feedstuffs. In ruminants, contaminated AFB1 feeds usually exhibit symptoms including reduced feed efficiency and milk production and decreased appetite. The objective of this study was to investigate the effects of different concentrations of AFB1 on rumen fermentation parameters by using the ANKOM gas production protocol. Rumen fluid was collected from a cannulated dry dairy cow, filtered with cheese-cloth and diluted (1:1) with a standard buffer. Triplicates of 75 mL flasks were fed 0,75g of feed (79% corn silage, 15% alfalfa and 6% concentrates) and inoculated with 0 (blank), 0,2, 0,5, 1 and 2 ppm of AFB1. Flasks were placed in a rotating incubation at 39°C for 96h and connected to ANKOM GP system. After 96h of incubation, the contents of each flask were centrifuged. Supernatants were analyzed for total VFA and AFB1 while precipitates were dried at 104°C for DM disappearance. The experimentation was repeated weekly 3 times and named wk1, 2 and 3. Statistical analysis was done by SPSS using a univariate model. Results showed no significant differences on GP max at 96h among AFB1 concentrations. Only wk 1 showed that higher AFB1 concentration (2 ppm) decreased significantly (P &lt; 0,05) DM disappearance (- 8,2 pts) when compared to the blank. Total VFA contents (75,0 ± 1,6 mM) were not affected by AFB1. Wk 2 and 3 did not show any difference neither on DM disappearance nor on VFA (89,1 ± 1,6 mM; 110,2 ± 4,8 mM). It can be concluded that our in vitro model, GP did not reflect DM disappearance and it can be put forward that rumen fluid with low total VFA concentrations (≤ 75 mM) could be more sensible to AFB1 challenge.

scholarly journals Models Based on the Mitscherlich Equation for Describing Typical and Atypical Gas Production Profiles Obtained from In Vitro Digestibility Studies Using Equine Faecal Inoculum

Animals ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 308
Author(s):  
Christopher D. Powell ◽  
Mewa S. Dhanoa ◽  
Anna Garber ◽  
Jo-Anne M. D. Murray ◽  
Secundino López ◽  
...  
Keyword(s):  
Goodness Of Fit ◽  
Nutritive Value ◽  
Gas Production ◽  
In Vitro Digestibility ◽  
Passage Rate ◽  
Statistical Measures ◽  
Mitscherlich Equation ◽  
Digesta Passage ◽  
Fermentation Parameters

Two models are proposed to describe atypical biphasic gas production profiles obtained from in vitro digestibility studies. The models are extensions of the standard Mitscherlich equation, comprising either two Mitscherlich terms or one Mitscherlich and one linear term. Two models that describe typical monophasic gas production curves, the standard Mitscherlich and the France model [a generalised Mitscherlich (root-t) equation], were assessed for comparison. Models were fitted to 25 gas production profiles resulting from incubating feedstuffs with faecal inocula from equines. Seventeen profiles displayed atypical biphasic patterns while the other eight displayed typical monophasic patterns. Models were evaluated using statistical measures of goodness-of-fit and by analysis of residuals. Good agreement was found between observed atypical profiles values and fitted values obtained with the two biphasic models, and both can revert to a simple Mitscherlich allowing them to describe typical monophasic profiles. The models contain kinetic fermentation parameters that can be used in conjunction with substrate degradability information and digesta passage rate to calculate extent of substrate degradation in the rumen or hindgut. Thus, models link the in vitro gas production technique to nutrient supply in the animal by providing information relating to digestion and nutritive value of feedstuffs.

Effect of variety and maturity stage on chemical composition, carbohydrate and protein subfractions, in vitro rumen degradability and energy values of timothy and alfalfa

2003 ◽  
Vol 83 (2) ◽  
pp. 279-290 ◽  
Author(s):  
P. Yu ◽  
D. A. Christensen ◽  
J. J. McKinnon ◽  
J. D. Markert
Keyword(s):  
Chemical Composition ◽  
Nutritive Value ◽  
Energy Content ◽  
Climatic Conditions ◽  
Phleum Pratense ◽  
Maturity Stage ◽  
Insoluble Protein ◽  
Timothy 2 ◽  
Acid Detergent Fibre

The objective of this study was to investigate the effects of forage variety and stage of maturity on chemical composition, protein and carbohydrate subfractions, in vitro rumen degradability and energy content under the climatic conditions of western Canada. Two varieties of alfalfa (Medicago sativa L. cvs. Pioneer and Beaver) and timothy (Phleum pratense L. cvs. Climax and Joliette), grown at three locations in Saskatchewan (Canada), were cut at three stages [1 = 1 wk before commercial cut (early bud for alfalfa; joint for timothy); 2 = at commercial cut (late bud for alfalfa; pre-bloom head for timothy); and 3 = one week after commercial cut (early bloom for alfalfa; full head for timothy)]. The results showed that: (1) the varieties had minimal effects on nutritional value; however, (2) stage of cutting had a large impact on chemical composition, protein and carbohydrate fractions, in vitro rumen degradability and energy value. As plant maturity advanced from stage 1 to 3, the following changes were noticed: (a) CP was decreased [alfalfa: 20.2 to 17.6; timothy: 11.1 to 7.9% dry matter (DM)]; fibre was affected very little in alfalfa but increased in timothy [acid detergent fibre (ADF): 38.0 to 40.7; neutral detergent fibre (NDF): 68.7 to 72.8% DM]; lignin and neutral detergent insoluble protein (NDIP) were not affected in either forage; acid detergent insoluble protein (ADIP) was increased in alfalfa (3.5 to 17.1% CP), but no effect was seen in timothy; (b) the rapidly degradable protein fraction (PA) was reduced in alfalfa (51.2 to 34.8% CP) but increased in timothy (9.2 to 27.5% CP); the rapidly degradable fraction (PB1) increased in alfalfa (0.0 to 25.2% CP), but decreased in timothy (31.6 to 16.7%); the intermediate degradable fraction (PB2) decreased in both forages (alfalfa: 14.1 to 5.2; timothy: 37.8 to 29.4% CP); the slowly degradable fraction (PB3) declined in alfalfa (33.1 to 17.7% CP), but was not affected in timothy (averaging 21.3% CP); the unavailable fraction (PC) associated with the cell wall increased in alfalfa (3.5 to 17.1% CP), but did not change in timothy (averaging 5.2% CP); (c) in vitro rumen degradabilities of DM (IVDMD) and NDF (IVNDFD) increased at stage 2 and then declined at stage 3. The highest IVDMD and IVNDFD after a 48-h incubation were at cutting stage 2 (50.5 and 37.3%; 42.8 and 37.3% for alfalfa and timothy, respectively); (d) the energy content behaved in a quadratic fashion; it increased initially and then decreased with the highest energy contents at stage 2 for both forages (TDN1x, DE1x, DEp, MEp, NELp: 63.8% DM, 2.92, 2.68, 2.26, 1.40 Mcal kg-1 DM for alfalfa; 45.3% DM, 2.03, 1.86, 1.43, 0.82 Mcal kg-1 DM for timothy, respectively). In general, the best cutting was found at stage 2, which was late-bud for alfalfa varieties and pre-bloom head for timothy. Key words: Alfalfa, timothy, variety, maturity, nutritive value

scholarly journals Comparison of three different rumen fluid as a source of inoculum to evaluate in vitro gas production and digestibility of elephant grass-concentrate mixture

2021 ◽  
Vol 888 (1) ◽  
pp. 012076
Author(s):  
H Soetanto ◽  
RM Aprilia ◽  
MS Pramita ◽  
I Banna
Keyword(s):  
Dry Matter ◽  
Nutritive Value ◽  
Rumen Fluid ◽  
Gas Production ◽  
Elephant Grass ◽  
Buffer Solution ◽  
Cattle Breeds ◽  
In Vitro Gas Production ◽  
Concentrate Mixture

Abstract This study aimed at elucidating the use of three different rumen fluid (RF) of indigenous cattle breeds i.e. Bali, Madura and Crossbred Ongole immediately after slaughtered at abattoir to evaluate the nutritive value of elephant grass( EG) -concentrate mixture using a standard in vitro gas production (IVGP) technique. Approximately 500 mg feed dry matter/syringe was added with 50 ml RF-buffer solution and incubated in a 39 0C water bath for 48 hours where gas production was observed at time intervals. Following termination of incubation the content was transferred into tare glass crucible to measure rumen dry matter (RDMD) and organic matter (ROMD) digestibility. The results showed that there was no significant different (P>0.05) in gas production parameters. In contrast, RDMD and ROMD differed significantly (P<0.01) among cattle breeds. RF from OCB resulted in the highest IVGP, RDMD and ROMD as compared with other RF sources. In conclusion, the use of RF from abattoir for IVGP measurement can be warranted using the same source of RF. The highest values resulted from OCB suggests that the abundance and variation in rumen microbiota may exist among cattle breeds.

scholarly journals Nutritional contents of processed Delonix regia seeds incubated in vitro with Pennisetum purpureum

2020 ◽  
Vol 45 (1) ◽  
pp. 309-316
Author(s):  
O. O. Olufayo ◽  
O. O. Falola
Keyword(s):  
Dry Matter ◽  
Nutritive Value ◽  
Short Chain Fatty Acids ◽  
Gas Production ◽  
Metabolizable Energy ◽  
Pennisetum Purpureum ◽  
Crude Fibre ◽  
Delonix Regia ◽  
Dry Matter Digestibility

The effect of processing on proximate, mineral, anti-nutritional compositions, gasproduction characteristics, methane production, predicted metabolizable energy, organic matter digestibility and short chain fatty acids production of Delonix regia seeds incubated with Pennisetum purpureum were determined. Five treatments (T1: mixture of soaked Delonix regia seed and Pennisetum purpureum; T2: mixture of raw Delonix regia seed and Pennisetum purpureum; T3: mixture of roasted Delonix regia seed and Pennisetum purpureum; T4: mixture of boiled Delonix regia seed and Pennisetum purpureum and T5:100% Pennisetum purpureum which serves as control) were designed. In vitro gas production techniques for 24 hours were used to determine the nutritive value of processed 50% Delonix regia seed and 50% Pennisetum purpureum. The crude protein ranged from 13.38 to 15.71g/100g DM among the treatments while crude fibre was between 20.10 and 21.40g/100g DM, ether extract ranged from 1.90 to 3.12g/100g DM and ash 7.77 to 12.34g/100g DM. Calcium ranged from (2.34 - 3.61%), magnesium ranged (2.90 – 3.48%), sodium (2.05 – 2.91%) and phosphorus (0.88 – 1.12%). It was observed generally that T2 recorded the highest among the treatments while the values obtained for the heat treated seeds were lower than the raw. Oxalates ranged between (0.23% - 0.32%), phytates (0.29 – 0.43%), tannin (0.03 – 0.08%) and saponnin (0.36 – 0.49%). Methane (ml/200mg DM) production indicated T3 (50% roasted Delonix regia seed and 50% Pennisetum purpureum) was highest. The potential gas production 'a+b' ranged from 12.33 to 28.33mL/200mg DM). The highest potential gas production 'a+b' value of 28.33mL/200mg DM was obtained in T1 compared to other dietary treatment. Dry matter digestibility (DMD) ranged between 70.23 – 91.02% while the rate of fermentation was between 0.51 and 1.18ml/hr. The rate of fermentation was directly proportional to dry matter digestibility (DMD); the soaked recorded the highest value. Processing enhanced the nutritional contents of Delonix regia seeds. The result obtained showed that there were significant differences (p<0.05) among the treatments. In conclusion, soaking the seeds of Delonix regia had beter nutritional contents and has the potential for dry season feeding.  

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