scholarly journals Design and implementation of a microfluidic device capable of temporal growth factor delivery reveal filtering capabilities of the EGFR/ERK pathway

2021 ◽  
Vol 5 (4) ◽  
pp. 046101
Author(s):  
Harris B. Krause ◽  
Hanna Bondarowicz ◽  
Alexis L. Karls ◽  
Megan N. McClean ◽  
Pamela K. Kreeger
Keyword(s):  
Growth Factor ◽  
Microfluidic Device ◽  
Erk Pathway ◽  
Growth Factor Delivery ◽  
Design And Implementation

Regulated Nerve Growth Factor Delivery on Novel Polymers by hNGF-EcR-293-TK Cells

2006 ◽  
Vol 22 (06) ◽  
Author(s):  
Sanjay Dhar ◽  
Delia Radulescu ◽  
Nareg Gharibjanian ◽  
Donald Hayes ◽  
Gregory Evans
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Growth Factor Delivery ◽  
Nerve Growth

Triple Growth Factor Delivery Promotes Functional Bone Regeneration Following Composite Musculoskeletal Trauma

2021 ◽  
Author(s):  
Ramesh Subbiah ◽  
Marissa A. Ruehle ◽  
Brett S. Klosterhoff ◽  
Angela S.P. Lin ◽  
Marian H. Hettiaratchi ◽  
...  
Keyword(s):  
Growth Factor ◽  
Bone Regeneration ◽  
Growth Factor Delivery ◽  
Musculoskeletal Trauma

scholarly journals Insulin-like growth factor-I signaling is modified during chondrocyte differentiation

2004 ◽  
Vol 183 (3) ◽  
pp. 477-486 ◽  
Author(s):  
Chanika Phornphutkul ◽  
Ke-Ying Wu ◽  
Xu Yang ◽  
Qian Chen ◽  
Philip A Gruppuso
Keyword(s):  
Growth Factor ◽  
Cell Line ◽  
Chondrocyte Differentiation ◽  
Erk Pathway ◽  
Insulin Like Growth Factor ◽  
Erk Activation ◽  
Atdc5 Cell ◽  
Factor I ◽  
Proliferation And Differentiation ◽  
Igf I

Insulin-like growth factor-I (IGF-I) is a critical regulator of skeletal growth. While IGF-I has been shown to be a potent chondrocyte mitogen in vitro, its role in chondrocyte differentiation is less well characterized. We chose to study the action of IGF-I on an accepted model of chondrocyte differentiation, the ATDC5 cell line. Insulin concentrations sufficiently high to interact with the IGF-I receptor are routinely used to induce ATDC5 cells to differentiate. Therefore, we first examined the ability of IGF-I to promote chondrocyte differentiation at physiological concentrations. IGF-I could induce differentiation of these cells at concentrations below 10 nM. However, increasing IGF-I concentrations were less potent at inducing differentiation. We hypothesized that mitogenic effects of IGF-I might inhibit its differentiating effects. Indeed, the extracellular-signal-regulated kinase (ERK)-pathway inhibitor PD98059 inhibited ATDC5 cell DNA synthesis while enhancing differentiation. This suggested that the ability of IGF-I to promote both proliferation and differentiation might require that its signaling be modulated through the differentiation process. We therefore compared IGF-I-mediated ERK activation in proliferating and hypertrophic chondrocytes. IGF-I potently induced ERK activation in proliferating cells, but minimal ERK response was seen in hypertrophic cells. In contrast, IGF-I-mediated Akt activation was unchanged by differentiation, indicating intact upstream IGF-I receptor signaling. Similar findings were observed in the RCJ3.1C5.18 chondrogenic cell line and in primary chick chondrocytes. We conclude that IGF-I promotes both proliferation and differentiation of chondrocytes and that the differentiation effects of IGF-I may require uncoupling of signaling to the ERK pathway.

Growth Factor Delivery Approaches in Hydrogels

2009 ◽  
Vol 10 (1) ◽  
pp. 9-18 ◽  
Author(s):  
Amanda K. Andriola Silva ◽  
Cyrille Richard ◽  
Michel Bessodes ◽  
Daniel Scherman ◽  
Otto-Wilhelm Merten
Keyword(s):  
Growth Factor ◽  
Growth Factor Delivery
Sign in / Sign up

Export Citation Format

Share Document