An integrated microfluidic platform to fabricate single-micrometer asymmetric giant unilamellar vesicles (GUVs) using dielectrophoretic separation of microemulsions

2021 ◽  
Vol 15 (2) ◽  
pp. 024112
Author(s):  
Sepehr Maktabi ◽  
Noah Malmstadt ◽  
Jeffrey W. Schertzer ◽  
Paul R. Chiarot
Keyword(s):  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles ◽  
Microfluidic Platform

scholarly journals Studying the effects of asymmetry on the bending rigidity of lipid membranes formed by microfluidics

2016 ◽  
Vol 52 (30) ◽  
pp. 5277-5280 ◽  
Author(s):  
K. Karamdad ◽  
R. V. Law ◽  
J. M. Seddon ◽  
N. J. Brooks ◽  
O. Ces
Keyword(s):  
Mechanical Properties ◽  
High Throughput ◽  
Lipid Membranes ◽  
Bending Rigidity ◽  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles ◽  
Microfluidic Platform

In this article we detail a robust high-throughput microfluidic platform capable of fabricating either symmetric or asymmetric giant unilamellar vesicles (GUVs) and characterise the mechanical properties of their membranes.

scholarly journals Effect of the Membrane Composition of Giant Unilamellar Vesicles on Their Budding Probability: A Trade-Off between Elasticity and Preferred Area Difference

Life ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 634
Author(s):  
Ylenia Miele ◽  
Gábor Holló ◽  
István Lagzi ◽  
Federico Rossi
Keyword(s):  
Phospholipid Fatty Acid ◽  
Enzymatic Reaction ◽  
Stimuli Responsive ◽  
Membrane Composition ◽  
Giant Unilamellar Vesicles ◽  
Physical Processes ◽  
Unilamellar Vesicles ◽  
Division Process ◽  
The Origin Of Life ◽  
Area Difference

The budding and division of artificial cells engineered from vesicles and droplets have gained much attention in the past few decades due to an increased interest in designing stimuli-responsive synthetic systems. Proper control of the division process is one of the main challenges in the field of synthetic biology and, especially in the context of the origin of life studies, it would be helpful to look for the simplest chemical and physical processes likely at play in prebiotic conditions. Here we show that pH-sensitive giant unilamellar vesicles composed of mixed phospholipid/fatty acid membranes undergo a budding process, internally fuelled by the urea–urease enzymatic reaction, only for a given range of the membrane composition. A gentle interplay between the effects of the membrane composition on the elasticity and the preferred area difference of the bilayer is responsible for the existence of a narrow range of membrane composition yielding a high probability for budding of the vesicles.

Domain Sorting in Giant Unilamellar Vesicles Adsorbed on Glass

Langmuir ◽  
2021 ◽  
Vol 37 (3) ◽  
pp. 1082-1088
Author(s):  
Chiho Kataoka-Hamai ◽  
Kohsaku Kawakami
Keyword(s):  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles

scholarly journals Production of giant unilamellar vesicles and encapsulation of lyotropic nematic liquid crystals

Soft Matter ◽  
2021 ◽  
Author(s):  
Peng Bao ◽  
Daniel A. Paterson ◽  
Sally A. Peyman ◽  
J. Cliff Jones ◽  
Jonathan A. T. Sandoe ◽  
...  
Keyword(s):  
Liquid Crystals ◽  
Nematic Liquid Crystals ◽  
Double Emulsion ◽  
Lyotropic Liquid Crystals ◽  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles ◽  
Emulsion Droplets ◽  
Double Emulsion Droplets

We describe a modified microfluidic method for making Giant Unilamellar Vesicles (GUVs) via water/octanol-lipid/water double emulsion droplets and encapsulation of nematic lyotropic liquid crystals (LNLCs).

scholarly journals Inter- and Intra-Plane Interactions Control the Existence of Macrodomains in Asymmetric Giant Unilamellar Vesicles

2021 ◽  
Vol 120 (3) ◽  
pp. 147a
Author(s):  
Thais A. Enoki ◽  
Haden L. Scott ◽  
Gerald W. Feigenson ◽  
Frederick A. Heberle
Keyword(s):  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles

Ciprofloxacin Encapsulation Into Giant Unilamellar Vesicles: Membrane Binding and Release

2013 ◽  
Vol 102 (2) ◽  
pp. 694-705 ◽  
Author(s):  
Nóra Kaszás ◽  
Tamás Bozó ◽  
Marianna Budai ◽  
Pál Gróf
Keyword(s):  
Membrane Binding ◽  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles

Protein Reconstitution Inside Giant Unilamellar Vesicles

2021 ◽  
Vol 50 (1) ◽  
Author(s):  
Thomas Litschel ◽  
Petra Schwille
Keyword(s):  
Optical Resolution ◽  
Microscopic Investigation ◽  
Annual Review ◽  
Publication Date ◽  
Protein Encapsulation ◽  
Resolution Limit ◽  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles ◽  
Encapsulation Methods ◽  
Selection Of

Giant unilamellar vesicles (GUVs) have gained great popularity as mimicries for cellular membranes. As their sizes are comfortably above the optical resolution limit, and their lipid composition is easily controlled, they are ideal for quantitative light microscopic investigation of dynamic processes in and on membranes. However, reconstitution of functional proteins into the lumen or the GUV membrane itself has proven technically challenging. In recent years, a selection of techniques has been introduced that tremendously improve GUV-assay development and enable the precise investigation of protein–membrane interactions under well-controlled conditions. Moreover, due to these methodological advances, GUVs are considered important candidates as protocells in bottom-up synthetic biology. In this review, we discuss the state of the art of the most important vesicle production and protein encapsulation methods and highlight some key protein systems whose functional reconstitution has advanced the field. Expected final online publication date for the Annual Review of Biophysics, Volume 50 is May 2021. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

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