Imaging ellipsometry for structured and plasmonic materials

2021 ◽  
Vol 129 (11) ◽  
pp. 113101
Author(s):  
Kurt Hingerl
Biosensors ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 164
Author(s):  
Haoyu Liu ◽  
Wei Liu ◽  
Gang Jin

Exosomes are a kind of membrane-bound phospholipid nanovesicle that are secreted extensively in a variety of biological fluids. Accumulating evidence has indicated that exosomes not only communicate with cells, but also perform functional roles in physiology and pathology. In addition, exosomes have also elicited a great deal of excitement due to their potential as disease biomarkers. Therefore, requirements for sensitive methods capable of precisely and specifically determining exosomes were needed. Herein, we not only develop a sensing surface to capture exosomes but also compare two surface proteins on exosomes, which are appropriate for detecting exosome surface markers by total internal reflected imaging ellipsometry (TIRIE). Protein G and antibody were immobilized on a thin layer of golden substrate to form the biosensing surface. The bio-interaction between antibodies and exosomes was recorded by the TIRIE in real time. The distance between exosomes adhered on a surface was 44 nm ± 0.5 nm. The KD  of anti-CD9 and exosome was lower than anti-CD63 and exosome by introducing pseudo-first-order interaction kinetics, which suggested that CD9 is more suitable for exosome surface markers than CD63. The limit of detection (LOD) of TIRIE was 0.4 μg/mL. In conclusion, we have proposed a surface for the detection of exosomes based on TIRIE, which can make the detection of exosomes convenient and efficient.


Nanoscale ◽  
2021 ◽  
Author(s):  
Mithun K P ◽  
Srabani Kar ◽  
Abinash Kumar ◽  
Victor Suvisesha Muthu Dharmaraj ◽  
Ravishankar Narayanan ◽  
...  

Collective excitation of Dirac plasmons in graphene and topological insulators have opened new possibilities of tunable plasmonic materials ranging from THz to mid-infrared regions. Using time resolved Optical Pump -...


2010 ◽  
Vol 22 (10) ◽  
pp. 1102-1110 ◽  
Author(s):  
Jimin Yao ◽  
An-Phong Le ◽  
Stephen K. Gray ◽  
Jeffrey S. Moore ◽  
John A. Rogers ◽  
...  
Keyword(s):  

2013 ◽  
Vol 577 ◽  
pp. 581-586 ◽  
Author(s):  
V.J. Keast ◽  
B. Zwan ◽  
S. Supansomboon ◽  
M.B. Cortie ◽  
P.O.Å. Persson
Keyword(s):  

2007 ◽  
Vol 19 (22) ◽  
pp. 3771-3782 ◽  
Author(s):  
W. A. Murray ◽  
W. L. Barnes
Keyword(s):  

2014 ◽  
Vol 4 (1) ◽  
Author(s):  
A. K. Pradhan ◽  
R. M. Mundle ◽  
Kevin Santiago ◽  
J. R. Skuza ◽  
Bo Xiao ◽  
...  

2008 ◽  
Vol 112 (23) ◽  
pp. 8499-8506 ◽  
Author(s):  
Inhwa Jung ◽  
Matthias Vaupel ◽  
Matthew Pelton ◽  
Richard Piner ◽  
Dmitriy A. Dikin ◽  
...  

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