Habilitated growth and characterization of Cinnamon Zeylanicum mediated MnO nanospheres and its bactericidal activity

2020 ◽  
Author(s):  
J. Rinita ◽  
Riya Jose ◽  
N. S. Nirmala Jothi
Keyword(s):  
Bactericidal Activity ◽  
Cinnamon Zeylanicum

scholarly journals Synthesis, Characterization and Bactericidal Activity of a 4-Ammoniumbuthylstyrene-Based Random Copolymer

Polymers ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1140
Author(s):  
Silvana Alfei ◽  
Gabriella Piatti ◽  
Debora Caviglia ◽  
Anna Maria Schito
Keyword(s):  
Antibacterial Activity ◽  
Bactericidal Activity ◽  
Antimicrobial Agents ◽  
Water Solubility ◽  
Physicochemical Characterization ◽  
Random Copolymer ◽  
Water Soluble ◽  
Cationic Polymers ◽  
Severe Infections

The growing resistance of bacteria to current chemotherapy is a global concern that urgently requires new and effective antimicrobial agents, aimed at curing untreatable infection, reducing unacceptable healthcare costs and human mortality. Cationic polymers, that mimic antimicrobial cationic peptides, represent promising broad-spectrum agents, being less susceptible to develop resistance than low molecular weight antibiotics. We, thus, designed, and herein report, the synthesis and physicochemical characterization of a water-soluble cationic copolymer (P5), obtained by copolymerizing the laboratory-made monomer 4-ammoniumbuthylstyrene hydrochloride with di-methyl-acrylamide as uncharged diluent. The antibacterial activity of P5 was assessed against several multi-drug-resistant clinical isolates of both Gram-positive and Gram-negative species. Except for strains characterized by modifications of the membrane charge, most of the tested isolates were sensible to the new molecule. P5 showed remarkable antibacterial activity against several isolates of genera Enterococcus, Staphylococcus, Pseudomonas, Klebsiella, and against Escherichia coli, Acinetobacter baumannii and Stenotrophomonas maltophilia, displaying a minimum MIC value of 3.15 µM. In time-killing and turbidimetric studies, P5 displayed a rapid non-lytic bactericidal activity. Due to its water-solubility and wide bactericidal spectrum, P5 could represent a promising novel agent capable of overcoming severe infections sustained by bacteria resistant the presently available antibiotics.

Characterization of bactericidal activity of clindamycin against Bacteroides fragilis via kill curve methods.

1996 ◽  
Vol 40 (8) ◽  
pp. 1941-1944 ◽  
Author(s):  
M E Klepser ◽  
M A Banevicius ◽  
R Quintiliani ◽  
C H Nightingale
Keyword(s):  
Bactericidal Activity ◽  
Bacteroides Fragilis ◽  
Kill Curve

Kill curves were determined for five isolates of Bacteroides fragilis with clindamycin at concentrations equal to the MIC or to 4, 16, and 64 times the MIC. Examination of plots of log CFU per milliliter versus time revealed no association between the clindamycin concentration and the rate and extent of the bactericidal activity against B. fragilis at or below 64 times the MIC.

Screening and characterization of medicinal plants extracts with bactericidal activity against Streptococcus mutans

2019 ◽  
Vol 34 (18) ◽  
pp. 2672-2676 ◽  
Author(s):  
Alejandra Isabel Vargas-Segura ◽  
Sonia Yesenia Silva-Belmares ◽  
Elda Patricia Segura-Ceniceros ◽  
Juan Alberto Ascacio-Valdés ◽  
Luis Méndez-González ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Streptococcus Mutans ◽  
Bactericidal Activity

Characterization of antibodies in human serum against serogroup B N. meningitidis that block complement-dependent bactericidal activity

2010 ◽  
Vol 47 (13) ◽  
pp. 2249-2249 ◽  
Author(s):  
Tathagat Dutta Ray ◽  
Lisa A. Lewis ◽  
Sunita Gulati ◽  
Peter A. Rice ◽  
Sanjay Ram
Keyword(s):  
Human Serum ◽  
Bactericidal Activity

scholarly journals The region around residue 115 of human bactericidal/permeability-increasing protein is not involved in lipopolysaccharide binding or bactericidal activity. Chemical synthesis and expression of a gene coding for the active domain and characterization of recombinant proteins

1994 ◽  
Vol 298 (3) ◽  
pp. 711-718 ◽  
Author(s):  
S Y Qi ◽  
Y Li ◽  
C D O'Connor
Keyword(s):  
Bactericidal Activity ◽  
Polymorphonuclear Leucocytes ◽  
Recombinant Polypeptide ◽  
Outer Membranes ◽  
High Affinity ◽  
Gene Coding ◽  
High Concentrations ◽  
Derivatives Of ◽  
Lipopolysaccharide Binding

Bactericidal/permeability-increasing protein (BPI) is a potent antimicrobial agent produced by polymorphonuclear leucocytes that specifically interacts with and kills Gram-negative bacteria. An 825 bp gene determining the bactericidal N-terminal domain of human BPI was chemically synthesized and expressed as inclusion bodies in Escherichia coli. The recombinant polypeptide, BPI', was solubilized and conditions under which it folded to give the active protein were determined. Folding was critically dependent on the urea and salt concentrations as well as the pH. BPI' bound with high affinity to Salmonella typhimurium cells (apparent Kd = 36 nM), permeabilized their outer membranes to actinomycin D, specifically activated a synovial fluid phospholipase A2 and showed potent bactericidal activity. In contrast with the native protein, however, it could not be efficiently released from the cell surface by the addition of high concentrations of Mg2+ ions. Pre-incubation of the protein with lipopolysaccharide or trypsin prevented cytotoxicity. However, boiling BPI' immediately before its addition to cells did not block its bactericidal activity, suggesting that it may be able to function even when presented to cells in an unfolded form. A BPI' derivative, containing a 13-residue foreign antigenic determinant genetically inserted between Ala115 and Asp116, was also produced. The derivative was functional in the above assays and bound with high affinity to S. typhimurium (apparent Kd = 74 nM). These results imply that the region defined by these residues is not involved in the lipopolysaccharide-binding or bactericidal activities of BPI. The availability of functional, nonglycosylated recombinant derivatives of BPI should greatly aid detailed studies on its structure, interactions with lipopolysaccharide and mechanism of action.

scholarly journals Identification of duck liver-expressed antimicrobial peptide 2 and characterization of its bactericidal activity

2019 ◽  
Vol 32 (7) ◽  
pp. 1052-1061 ◽  
Author(s):  
Yeojin Hong ◽  
Anh Duc Truong ◽  
Janggeun Lee ◽  
Kyungbaek Lee ◽  
Geun-Bae Kim ◽  
...  
Keyword(s):  
Antimicrobial Peptide ◽  
Bactericidal Activity

scholarly journals Isolation and characterization of Escherichia coli phage and enhance its bactericidal activity that collaborative with kanamycin sulfate

2020 ◽  
Author(s):  
Liming Jiang ◽  
Rui Zheng
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
Bactericidal Activity ◽  
Therapeutic Potential ◽  
Health Concern ◽  
Low Concentration ◽  
E Coli ◽  
Kanamycin Sulfate ◽  
Isolation And Characterization

Abstract Background: Escherichia coli is the most important and widespread bacteria in worldwide, which mainly found in contaminated food, human and animal faeces. Unfortunately, Some of E. coli strains are multidrug-resistant (MDR) pathogen leading significant public health concern globally. Biofilm is a multicellular community of microorganisms. Phages and their derivatives are ideal candidates for replacing or compensating for antibiotic problems in the future. Method: Here, we aimed to isolation and characterization of Escherichia coli phage and research its bactericidal activity that individually or collaborative with kanamycin sulfateResults: In this study, three virulent phages Flora, T4 and WJ were isolated from the laboratory and drug sample in Wuxi, China. It’s belonged to the Myoviridae family and optimum temperature is 42 ℃, optimum pH= 7, optimum MOI is 0.0001 and the genome size of Flora, T4 and WJ were 168, 909, 168903 and 168, 900 bp respectively. Flora has two exonuclease, whereas T4 and WJ have only one. Antibiotics have better bactericidal activity than phages in a low concentration medium of bacteria, nonetheless, phages have better bactericidal activity than antibiotics in a high concentration of bacteria, and that, collaboration of phages and antibiotics have better bactericidal activity effect than alone of phages or antibiotics in a low concentration medium of bacteria. Conclusion: The excellent performance of phage Flora for its therapeutic potential on clinic. The data of this study provided the strong evidence that the application of phage could reduce the growth and biofilm of E. coli that are important to maintain public health. Keywords: Escherichia coli, phage, lytic spectrum, biofilm, antibiotic

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