scholarly journals Identification of duck liver-expressed antimicrobial peptide 2 and characterization of its bactericidal activity

2019 ◽  
Vol 32 (7) ◽  
pp. 1052-1061 ◽  
Author(s):  
Yeojin Hong ◽  
Anh Duc Truong ◽  
Janggeun Lee ◽  
Kyungbaek Lee ◽  
Geun-Bae Kim ◽  
...  
Keyword(s):  
Antimicrobial Peptide ◽  
Bactericidal Activity

scholarly journals Synthesis, Characterization and Bactericidal Activity of a 4-Ammoniumbuthylstyrene-Based Random Copolymer

Polymers ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1140
Author(s):  
Silvana Alfei ◽  
Gabriella Piatti ◽  
Debora Caviglia ◽  
Anna Maria Schito
Keyword(s):  
Antibacterial Activity ◽  
Bactericidal Activity ◽  
Antimicrobial Agents ◽  
Water Solubility ◽  
Physicochemical Characterization ◽  
Random Copolymer ◽  
Water Soluble ◽  
Cationic Polymers ◽  
Severe Infections

The growing resistance of bacteria to current chemotherapy is a global concern that urgently requires new and effective antimicrobial agents, aimed at curing untreatable infection, reducing unacceptable healthcare costs and human mortality. Cationic polymers, that mimic antimicrobial cationic peptides, represent promising broad-spectrum agents, being less susceptible to develop resistance than low molecular weight antibiotics. We, thus, designed, and herein report, the synthesis and physicochemical characterization of a water-soluble cationic copolymer (P5), obtained by copolymerizing the laboratory-made monomer 4-ammoniumbuthylstyrene hydrochloride with di-methyl-acrylamide as uncharged diluent. The antibacterial activity of P5 was assessed against several multi-drug-resistant clinical isolates of both Gram-positive and Gram-negative species. Except for strains characterized by modifications of the membrane charge, most of the tested isolates were sensible to the new molecule. P5 showed remarkable antibacterial activity against several isolates of genera Enterococcus, Staphylococcus, Pseudomonas, Klebsiella, and against Escherichia coli, Acinetobacter baumannii and Stenotrophomonas maltophilia, displaying a minimum MIC value of 3.15 µM. In time-killing and turbidimetric studies, P5 displayed a rapid non-lytic bactericidal activity. Due to its water-solubility and wide bactericidal spectrum, P5 could represent a promising novel agent capable of overcoming severe infections sustained by bacteria resistant the presently available antibiotics.

scholarly journals Beneficial Impacts of Incorporating the Non-Natural Amino Acid Azulenyl-Alanine into the Trp-Rich Antimicrobial Peptide buCATHL4B

Biomolecules ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 421
Author(s):  
Areetha R. D’Souza ◽  
Matthew R. Necelis ◽  
Alona Kulesha ◽  
Gregory A. Caputo ◽  
Olga V. Makhlynets
Keyword(s):  
Amino Acid ◽  
Antimicrobial Peptides ◽  
Antimicrobial Peptide ◽  
Mechanism Of Action ◽  
Bactericidal Activity ◽  
Antimicrobial Agents ◽  
Human Cells ◽  
Side Chains ◽  
Natural Amino Acid ◽  
Proteolytic Stability

Antimicrobial peptides (AMPs) present a promising scaffold for the development of potent antimicrobial agents. Substitution of tryptophan by non-natural amino acid Azulenyl-Alanine (AzAla) would allow studying the mechanism of action of AMPs by using unique properties of this amino acid, such as ability to be excited separately from tryptophan in a multi-Trp AMPs and environmental insensitivity. In this work, we investigate the effect of Trp→AzAla substitution in antimicrobial peptide buCATHL4B (contains three Trp side chains). We found that antimicrobial and bactericidal activity of the original peptide was preserved, while cytocompatibility with human cells and proteolytic stability was improved. We envision that AzAla will find applications as a tool for studies of the mechanism of action of AMPs. In addition, incorporation of this non-natural amino acid into AMP sequences could enhance their application properties.

scholarly journals Development and characterization of phosphatidylcholine nanovesicles containing the antimicrobial peptide nisin

2010 ◽  
Vol 43 (4) ◽  
pp. 1198-1203 ◽  
Author(s):  
Patrícia da Silva Malheiros ◽  
Yasmine Miguel Serafini Micheletto ◽  
Nádya Pesce da Silveira ◽  
Adriano Brandelli
Keyword(s):  
Antimicrobial Peptide

Synthesis and characterization of indolicidin, a tryptophan-rich antimicrobial peptide from bovine neutrophils *

2009 ◽  
Vol 45 (5) ◽  
pp. 401-409 ◽  
Author(s):  
ROBERT J. VAN ABEL ◽  
YI-QUAN TANG ◽  
V.S.V. RAO ◽  
CRAIG H. DOBBS ◽  
DAT TRAN ◽  
...  
Keyword(s):  
Antimicrobial Peptide ◽  
Synthesis And Characterization ◽  
Bovine Neutrophils

Characterization of bactericidal activity of clindamycin against Bacteroides fragilis via kill curve methods.

1996 ◽  
Vol 40 (8) ◽  
pp. 1941-1944 ◽  
Author(s):  
M E Klepser ◽  
M A Banevicius ◽  
R Quintiliani ◽  
C H Nightingale
Keyword(s):  
Bactericidal Activity ◽  
Bacteroides Fragilis ◽  
Kill Curve

Kill curves were determined for five isolates of Bacteroides fragilis with clindamycin at concentrations equal to the MIC or to 4, 16, and 64 times the MIC. Examination of plots of log CFU per milliliter versus time revealed no association between the clindamycin concentration and the rate and extent of the bactericidal activity against B. fragilis at or below 64 times the MIC.

Characterization of virus/double-stranded RNA-dependent induction of antimicrobial peptide hepcidin in trout macrophages

2007 ◽  
Vol 31 (12) ◽  
pp. 1297-1309 ◽  
Author(s):  
Pinwen Peter Chiou ◽  
Chun-Mean Lin ◽  
Niels C. Bols ◽  
Thomas T. Chen
Keyword(s):  
Antimicrobial Peptide ◽  
Double Stranded Rna

scholarly journals Purification and characterization of three isoforms of chrysophsin, a novel antimicrobial peptide in the gills of the red sea bream, Chrysophrys major

2003 ◽  
Vol 270 (4) ◽  
pp. 675-686 ◽  
Author(s):  
Noriaki Iijima ◽  
Norio Tanimoto ◽  
Yohko Emoto ◽  
Yohko Morita ◽  
Kazumasa Uematsu ◽  
...  
Keyword(s):  
Antimicrobial Peptide ◽  
Red Sea ◽  
Sea Bream ◽  
Red Sea Bream ◽  
Purification And Characterization
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