scholarly journals Platelet rich plasma (PRP) decrease COX-2 intratesticular and triglyceride serum levels of D-Galactose induced male Wistar rats (Rattus norvegicus)

2020 ◽  
Author(s):  
Dicky Moch. Rizal ◽  
Ahmad Hamim Sadewa ◽  
Dhinar Mustika Natalia
Keyword(s):  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Platelet Rich Plasma ◽  
Serum Levels ◽  
Male Wistar Rats ◽  
Cox 2

scholarly journals Effect of Caffeic Acid Phenethyl Ester Provision on Fibroblast Growth Factor-2, Matrix Metalloproteinase-9 Expression, Osteoclast and Osteoblast Numbers during Experimental Tooth Movement in Wistar Rats (Rattus norvegicus)

2021 ◽  
Author(s):  
Ida Bagus Narmada ◽  
Paristyawati Dwi Putri ◽  
Lucky Lucynda ◽  
Ari Triwardhani ◽  
I Gusti Aju Wahju Ardani ◽  
...  
Keyword(s):  
Growth Factor ◽  
Matrix Metalloproteinase ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Tooth Movement ◽  
Caffeic Acid Phenethyl Ester ◽  
Fibroblast Growth Factor 2 ◽  
Fibroblast Growth ◽  
Male Wistar Rats ◽  
Metalloproteinase 9

Abstract Objectives To investigate the effect of caffeic acid phenethyl ester (CAPE) provision on matrix metalloproteinase-9 (MMP-9), fibroblast growth factor-2 (FGF-2) expression, osteoclast and osteoblast numbers during experimental orthodontic tooth movement (OTM) in male Wistar rats (Rattus norvegicus). Materials and Methods Forty-eight healthy male Wistar rats (R. norvegicus), 16 to 20 weeks old with 200 to 250 g body weight (bw) were divided into several groups as follows: K1: OTM for 3 days; K2: OTM for 7 days; K3: OTM for 14 days; KP1: OTM and CAPE for 3 days; KP2: OTM and CAPE for 7 days; and KP3: OTM and CAPE for 14 days. A nickel titanium closed coil spring 8.0 mm long with 10 g/mm2 was installed between the upper left first molar and upper central incisor to move molar mesially. CAPE provision with a dose of 20 mg/kg bw of animal studies was done per orally. Immunohistochemistry was done to examine MMP-9 expression and osteoclast number in compression side as well as FGF-2 expression and osteoblast number in tensile side of the OTM. Statistical Analysis One-way analysis of variance test and Tukey’s honest significant difference test were performed to determine the difference between the groups (p < 0.05). Results MMP-9 expression and osteoclast numbers in the compression side were significantly different between the groups. Similarly, FGF-2 expression and osteoclast numbers in the tensile side were significantly different between the groups. Conclusions CAPE provision during OTM increases the number of osteoblasts and the FGF-2 expression significantly in the tensile side. Osteoclast numbers and MMP-9 expression significantly decrease in the compression side.

scholarly journals Effect of different types of stress on adrenal gland parameters and adrenal hormones in the blood serum of male Wistar rats

2009 ◽  
Vol 61 (2) ◽  
pp. 187-194 ◽  
Author(s):  
M. Adzic ◽  
Ana Djordjevic ◽  
Jelena Djordjevic ◽  
Ana Niciforovic ◽  
Marija Radojcic
Keyword(s):  
Blood Serum ◽  
Wistar Rats ◽  
Adrenal Glands ◽  
Acute Stress ◽  
Serum Levels ◽  
Combined Stress ◽  
Adrenal Hormones ◽  
General Adaptation ◽  
Different Types ◽  
Male Wistar Rats

In the present study, we examined gross changes in the mass of whole adrenal glands and that of the adrenal cortex and medulla in mature male Wistar rats subjected to three different stress types: acute, chronic, and combined, i.e., chronic followed by acute stress. These parameters were correlated with adrenal activity as judged from serum levels of corticosterone and catecholamine, respectively, as well as with serum levels of ACTH and glucose. Under all three conditions, we observed bilaterally asymmetric and stress-type-independent hypertrophy of whole adrenals, as well as adrenal cortices and medullas. Under acute and combined stress, adrenal hypertrophy was followed by increase of adrenal hormones in the blood serum. However, under chronic stress, both cortical and medullar activities as judged from low or unaltered levels of the respective hormones and glucose were compromised and disconnected from the input signal of ACTH. Since all of the studied adrenal activities could be restored by subsequent acute stress, it is concluded that chronic isolation can be viewed as partly maladaptive stress with characteristics resembling stress resistance rather than the stress exhaustion stage of the general adaptation syndrome.

scholarly journals Antioxidant status and hepato-protective role of Anchomanes difformis in streptozotocin-induced diabetes in male Wistar rats

2020 ◽  
Vol 66 (1) ◽  
pp. 18-36
Author(s):  
Toyin D. Alabi ◽  
Nicole L. Brooks ◽  
Oluwafemi O. Oguntibeju
Keyword(s):  
Type 2 Diabetes ◽  
Blood Glucose ◽  
Aqueous Extract ◽  
Liver Dysfunction ◽  
Antioxidant Status ◽  
Wistar Rats ◽  
Serum Levels ◽  
Male Wistar Rats ◽  
Hepatic Injuries

SummaryIntroduction: The liver is involved in the metabolism of xenobiotics and their metabolites and it is vulnerable to oxidative damage. Hyperglycaemia is highly implicated in the progression of diabetes mellitus, and adversely affects the liver. Though, conventional hypoglycaemic drugs may be effective in reducing blood glucose, they do not appear to be effective in attenuating the progression of diabetes and its complications.Objective: This study evaluated the ameliorative effects of Anchomanes difformis on hyperglycaemia and hepatic injuries in type 2 diabetes.Methods: Type 2 diabetes was induced in male Wistar rats with a single intraperitoneal injection of streptozotocin (40 mg/kg BW) after two weeks of fructose (10%) administration. Aqueous extract of A. difformis (200 and 400 mg/kg BW) and glibenclamide (5 mg/kg BW) were administered orally for six weeks. Blood glucose concentrations were measured. Serum levels of liver dysfunction markers (ALT, AST, and ALP), total cholesterol, triglycerides, HDL- and LDL-cholesterol were investigated. Total protein, albumin, and globulin were also assessed. Antioxidant parameters: ORAC, GSH, GSSG, SOD, CAT and FRAP were evaluated in the liver while ORAC, FRAP and lipid peroxidation were determined in the serum. Histological examination of the liver tissue was carried out.Results: Treatment with aqueous extract of A. difformis significantly (p<0.05) reduced blood glucose and reversed steatosis in the diabetic-treated rats. The antioxidant status of diabetic-treated rats was significantly (p<0.05) improved. Serum levels of liver dysfunction markers were significantly (p<0.05) reduced in diabetic-treated rats.Conclusion: The findings in this study revealed that 400 mg/kgBW Anchomanes difformis was more effective than 200 mg/kg BW in ameliorating diabetes-induced hepatopathy, however, both doses of Anchomanes difformis demonstrated more antidiabetic ability than glibenclamide. Anchomanes difformis may be a novel and potential therapeutic agent in the management of diabetes and resulted hepatic injuries.

scholarly journals Ekstrak Etanol Daun Jati Belanda (Guazuma ulmifolia Lamk) Memperbaiki Profil Lipid Tikus (Rattus norvegicus) Wistar Jantan Dislipidemia

2020 ◽  
Vol 1 (1) ◽  
pp. 25-30
Author(s):  
Melita Hidajat ◽  
I Gusti Made Aman ◽  
Hendro Sukoco ◽  
Ferbian Milas Siswanto
Keyword(s):  
Treatment Group ◽  
Lipid Profile ◽  
Total Cholesterol ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Control Group ◽  
Wistar Strain ◽  
Before And After ◽  
Male Wistar Rats ◽  
Guazuma Ulmifolia

The purpose of this study was to prove that the administration of Jati (Guazuma ulmifolia Lamk) leaves extract improves the lipid profile of dyslipidemic male Wistar rats. Subjects were 20 rats (Rattus norvegicus), male, Wistar strain, dyslipidemia (total cholesterol ≥ 200 mg dl-1), aged 2 months old, weighing 180-200 grams. The control group (10 rats) were given a placebo of 3 ml aquadest (P0) and the treatment group was given extracts of the Jati (Guazuma ulmifolia Lamk) leaves extract of 25 mg kg-1 BW (P1). Before and after treatment for 14 days, total cholesterol, triglyceride, LDL, and HDL levels were examined. The results showed that in the P0 group there were no changes in total cholesterol, triglyceride, LDL, and HDL levels (p>0.05), whereas the P1 group experienced a decrease in total cholesterol, triglyceride and LDL levels (p<0.05) and an increase in HDL levels (p<0.05). The results of this study indicated that the Jati leaves extract was effective to improve the lipid profile of dyslipidemic rats. It was necessary to compare the effectiveness of Jati leaves extract with synthetic dyslipidemia drugs used in the community such as statin.

Chronic administration of gabapentin and a gabapentin-carbamazepine combination reversibly suppress testicular function in male Wistar rats ( Rattus norvegicus )

2017 ◽  
Vol 24 (2) ◽  
pp. 63-69 ◽  
Author(s):  
Opeyemi Samson Osuntokun ◽  
Gbola Olayiwola ◽  
Ayowole Oladele ◽  
Idowu Ola ◽  
Oludele AyokaAbiodun
Keyword(s):  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Chronic Administration ◽  
Testicular Function ◽  
Male Wistar Rats

scholarly journals Comparative studies on the hypercholesterolaemia induced by excess dietary tyrosine or polychlorinated biphenyls in rats

1986 ◽  
Vol 56 (2) ◽  
pp. 509-517 ◽  
Author(s):  
Sahoshi Nagaoka ◽  
Mitsuhiro Kato ◽  
Yoritaka Aoyama ◽  
Akira Yoshida†
Keyword(s):  
Ascorbic Acid ◽  
Polychlorinated Biphenyls ◽  
Comparative Studies ◽  
Wistar Rats ◽  
Serum Levels ◽  
Liver Lipids ◽  
Male Wistar Rats

1. The effects of dietary polychlorinated biphenyls (PCB) and excess tyrosine on serum and liver lipids, urinary ascorbic acid and catecholamines were compared in male Wistar rats.2. Serum levels of cholesterol, urinary ascorbic acid, norepinephrine, epinephrine, dopamine and histamine were significantly increased in rats given either PCB or excess tyrosine.3. The hypercholesterolaemia induced by PCB or excess tyrosine was blocked by the adrenergic α-blocker, phenoxybenzamine.4. The present results suggest causal interrelations between the hypercholesterolaemia induced by dietary PCB or excess tyrosine and the secretion of catecholamines.

scholarly journals Dehydration-induced increase in aquaporin-2 protein abundance is blocked by nonsteroidal anti-inflammatory drugs

2010 ◽  
Vol 298 (4) ◽  
pp. F1051-F1058 ◽  
Author(s):  
Erin Baggaley ◽  
Søren Nielsen ◽  
David Marples
Keyword(s):  
Wistar Rats ◽  
Hydration Status ◽  
Free Access ◽  
Protein Abundance ◽  
Aquaporin 2 ◽  
Male Wistar Rats ◽  
Access To Water ◽  
Inflammatory Drugs ◽  
Cox 2 ◽  
Selective Nsaid

It is now well established that the antidiuretic response to vasopressin is modulated by changes in aquaporin-2 (AQP2) expression in response to hydration status. While vasopressin itself is one signal driving expression, other signals also play a part. In this study, we planned to investigate whether prostaglandins, known to modulate AQP2 trafficking, may play a role in this process. Male Wistar rats were kept in metabolic cages, with either free access to water and food, or were given 15 g of food gelled with water, such that they were fluid restricted or fluid loaded. The effects of oral administration of two structurally different NSAIDs, indomethacin and ibuprofen, and a COX-2-selective NSAID, meloxicam, on urine output and AQP2 expression were investigated in kidneys removed under terminal anesthesia. All the NSAIDs decreased AQP2 expression significantly in water-restricted rats but did not significantly alter PGE excretion. In water-loaded rats, the effects were less marked, and meloxicam had no significant effect. Consistent with this, ibuprofen prevented the increase in AQP2 expression seen in response to dehydration. These results demonstrate that NSAIDs decrease AQP2 protein abundance, particularly during adaptation during dehydration. This may be of particular significance in older and critically ill patients, who are prone to dehydration.

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