The inhibitory effect of silver nanoparticles on someespecially bacteria microorganisms, Pesudomonas aeruginosa, Esherichia coli and Staphylococcus aureus

2020 ◽  
Author(s):  
Elham Jasim Mohammad ◽  
Aseel Mustafa Abdul Majeed ◽  
Jaffer Hashim Mohsin ◽  
Iman Hendi Gatea ◽  
Gailan H. Al-Tayar
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Inhibitory Effect ◽  
Esherichia Coli ◽  
And Staphylococcus Aureus

Antimicrobial and preservative effects of the combinations of nisin, tea polyphenols, rosemary extract and chitosan on pasteurized chicken sausage

2020 ◽  
Author(s):  
Kekui Sun ◽  
Shanglong Wang ◽  
Qingfeng Ge ◽  
Guoyuan Xiong ◽  
Xi Zhou ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Low Temperature ◽  
Shelf Life ◽  
Inhibitory Effect ◽  
Tea Polyphenols ◽  
Rosemary Extract ◽  
Clear Zone ◽  
Antioxidant Effects ◽  
And Staphylococcus Aureus

The study evaluated the antimicrobial and antioxidant effects of the combinations of nisin (NS), tea polyphenols (TP), rosemary extract (RE) and chitosan (CS) on low-temperature chicken sausage. An orthogonal test revealed that the most effective antimicrobial compositions were equal-quantity mixtures of 0.05% NS + 0.05% TP + 0.03% RE + 0.55% CS . The mixture also produced strong antimicrobial and antioxidant effects in low-temperature chicken sausage related to extend the shelf life to more than 30 days at 4°C. The study also investigated the inhibitory zone of NS, TP, RE and CS against Pseudomonas aeruginosa , lactic acid bacteria (LAB) and Staphylococcus aureus which were the dominant spoilage bacteria in low-temperature chicken sausage. NS had the greatest inhibitory effect on LAB and Staphylococcus aureus , exhibiting clear zone diameters of 19.7 mm and 17.8 mm respectively. TP had the largest inhibitory effect on Pseudomonas aeruginosa , exhibiting a clear zone diameter of 18.2 mm. These results indicated that the combination of NS, TP, RE and CS could be used as natural preservative s to efficiently inhibit the growth of spoilage microorganisms in low-temperature chicken sausage so as to improve its safety and shelf life.

Research on Properties of Antibacterial Paper Sprayed by Nano-Chitosan

2014 ◽  
Vol 926-930 ◽  
pp. 214-217
Author(s):  
Ying Hong ◽  
Li Ze Tang
Keyword(s):  
Staphylococcus Aureus ◽  
Tensile Strength ◽  
Antibacterial Effect ◽  
Circle Method ◽  
Inhibitory Effect ◽  
Bursting Strength ◽  
Obvious Change ◽  
And Staphylococcus Aureus ◽  
Better Than

Antibacterial paper for packaging was sprayed by nanochitosan as antibacterial ingredients. The bacteriostatic circle method was adopted to evaluate its antibacterial effect on E.coli and Staphylococcus aureus. We investigated the effects of different concentrations of nanochitosan on antibacterial paper thickness, tensile strength, bursting strength, folding strength and antibacterial properties.The results showed that: tensile strength, bursting strength and folding strength of the antibacterial paper is better than the control paper and the thickness has no obvious change. The antibacterial paper enhanced the antibacterial effect following the increasing concentration of nanochitosan. The same concentration of nanochitosan antibacterial paper, its inhibitory effect on E.coli was greater than that of Staphylococcus aureus.

scholarly journals IN VITRO ANTIBACTERIAL ACTIVITY OF BIOSYNTHESIZED SILVER NANOPARTICLES FROM ETHYL ACETATE EXTRACT OF HYDROCOTYLE SIBTHORPIODES AGAINST MULTI DRUG RESISTANT MICROBES.

2017 ◽  
Vol 10 (6) ◽  
pp. 263 ◽  
Author(s):  
Manashi Garg ◽  
Banasmita Devi ◽  
Rashna Devi
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Metallic Silver ◽  
Klebsiella Pneumonia ◽  
Synthesis Methods ◽  
Drug Resistant ◽  
Bacterial Isolates ◽  
And Staphylococcus Aureus

 Objectives: Hydrocotyle sibthorpiodes is known to contain several phytoconstituents which are constantly involved in the formation of Silver nanoparticles that may affect several multi-drug resistant microbes. Therefore, the study was undertaken to evaluate the efficacy of different concentration of nano silver solution on three bacterial isolates. It was also aimed to qualitatively assess the different phytoconstituents responsible for the synthesis. Methods: Three bacterial isolates of Klebsiella pneumonia, Pseudomonas aeroginosa and Staphylococcus aureus were identified. Synthesis of AgNPs with different concentration (2/4/6/8/10μl/ml) was done and applied to the selected isolates. The phytochemical compounds of the ethyl acetate extract were assayed by several colored reactions qualitatively. Results: The size and stability biosynthesis of the metallic silver nanoparticles were confirmed by photophysical characterization as well as SEM (Scanning Electron Microscopy), XRD (XRay Diffraction), Zeta potential and DLS (Dynamic Light Scattering) with an average size of 13.37 ±10 nm. The increasing concentration of the particle solution showed significant inhibition zone for all the three isolates viz., Klebsiella pneumonia, Pseudomonas aeroginosa and Staphylococcus. aureus showing the value of 3.0±0.17, 2.7±0.32 and 3.6±0.57 respectively for 10 μl/ml concentration. Phytochemical screening of the whole plant extract also revealed an array of bioactive compounds which may have an effective role in the reduction process. Conclusion: The study demonstrated a simple, efficient and eco-friendly synthesis of stable silver nanoparticles from the ethyl acetate extract of Hydrocotyle sibthorpiodes having fairly superior antimicrobial activity against human pathogens.

scholarly journals The Antibacterial Activity of Thermoactinomyces sp. (H24) Extract Against Escherichia coli and Staphylococcus aureus

2021 ◽  
Vol 3 (1) ◽  
pp. 56-63
Author(s):  
Julia Nanda Puspita ◽  
Rikhsan Kurniatuhadi ◽  
Rahmawati Rahmawati
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bioactive Compounds ◽  
Bioactive Compound ◽  
Inhibitory Effect ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
And Staphylococcus Aureus

Bacteria of the genus Thermoactinomyces have the ability to produce antibacterial bioactive compounds. This bioactive compound can be used for combating diarrheal agents such as Escherichia coli and Staphylococcus aureus. This study aims to determine the antibacterial activity of the metabolite extract from Thermoactinomyces sp. (H24) against Escherichia coli and Staphylococcus aureus. Methanol was used as a solvent for the extraction of bacterial bioactive compounds. Antibacterial activity was analyzed by the diffusion method with several extract concentrations (0.75 mL, 1.5 mL, 2.25 mL, and 3 mL), 10% DMSO as the negative control, and ciprofloxacin as the positive control. Our result shows that Termoactinomyces sp. (H24) extract has an inhibitory effect on the growth of  E. coli and S. aureus with an effective concentration of 2.25 mL (inhibition strength: very strong).

scholarly journals UJI AKTIVITAS KOMBINASI EKSTRAK KERING LIDAH BUAYA (Aloe vera. (L) brum. f.) DAN EKSTRAK KENTAL DAUN SIRIH MERAH (Piper crocatum ruiz & pav) UNTUK ANTIBAKTERI PENYEBAB JERAWAT

2018 ◽  
Vol 15 (01) ◽  
pp. 12
Author(s):  
Suhaimi Suhaimi ◽  
Teti Indrawati ◽  
Shirly Kumala
Keyword(s):  
Staphylococcus Aureus ◽  
Leaf Extract ◽  
Propionibacterium Acnes ◽  
Aloe Vera ◽  
Inhibitory Effect ◽  
Dry Extract ◽  
Propionibacterium Acne ◽  
Betel Leaf ◽  
And Staphylococcus Aureus

INTISARI   Ekstrak kering lidah buaya dan ekstrak kental daun sirih merah mengandung senyawa alkaloid, flavonoid, tanin, saponin dan fenol yang mempunyai aktivitas sebagai antibakteri penyebab jerawat. Penelitian ini bertujuanUntuk menentukan konsentrasi ekstrak kering lidah buaya  (Aloe vera (L) brum f), untuk menentukan ekstrak kental daun sirih merah (Piper crotacum ruiz dan pav)  mempunyai daya hambat ter terhadap bakteri Propionibacterium acnes dan Staphylococcus aureus penyebab jerawat dan untuk menentukan konsentrasi kombinasi ekstrak kering lidah buaya  (Aloe vera (L) brum f)  dan ekstrak kental daun sirih merah  (Piper crotacum ruiz dan pav)mempunyai daya hambat terterhadap bakteri  Propionibacterium acnes  dan  Staphylococcus aureus  penyebab jerawat.Tahap-tahap penelitian yaitu determinasi tanaman lidah buaya dan daun sirih merah, pembuatan ekstrak, pemeriksaan ekstrak meliputi organoleptik, pH, uji skrining fitokimia serta pengujian aktivitas masing-masing ekstrak kering lidah buaya dengan konsentrasi 10%, 15%, 20%, 25%, 30% dan 35%, ekstrak kental daun sirih merah dengan konsentrasi 25%, 12,5%, 7,16%, 3,13%, 1,78% dan 0,78% serta kombinasi ekstrak kering lidah buaya dan ekstrak kental daun sirih merah dengan konsentrasi 50%, 25%, 12,5%, 7,16%, 3,13%,  dan 1,56 terhadap bakteri Propionibacterium acnes dan Staphylococcus aureus. Kombinasi ekstrak kering  lidah buaya  dan ekstrak kental daun sirih merah   memiliki aktivitas antibakteri terhadap bakteri  Staphylococcus aureus  pada konsentrasi 1,56 % dan Propionibacterium acne   pada konsentrasi  3,13  Kata kunci : Lidah buaya, daun sirih merah, Propionibacterium acnes, Staphylococcus aureus.  ABSTRACT  Dried aloe vera extract and thick red betel leaf extract contains alkaloid compounds, flavonoids, tannins, saponins and phenols that have activity as an antibacterial cause of acne. The aim of this study was to determine the concentration of Aloe vera (L) brum f) extract, to determine the thick red betel leaf extract (Piper crotacum ruiz and pav) to have the inhibitory effect against the bacteria Propionibacterium acnes and Staphylococcus aureus causes acne and  to determine the concentration of Aloe vera (L) brum f) and red thick betel leaf extract (Piper crotacum ruiz and pav) have inhibitory effect on bacteria Propionibacterium acnes and Staphylococcus aureus cause acne. The research stages are the determination of aloe vera and red betel leaf, extract preparation, extract examination including organoleptic, pH, phytochemical screening test and activity test of dry aloe vera extract with concentration 10%, 15%, 20%, 25% , 30% and 35%, thick red betel leaf extract with concentration of 25%, 12.5%, 7.16%, 3.13%, 1.78% and 0.78%, and combination of aloe vera extract and viscous extract red betel leaves with concentrations of 50%, 25%, 12.5%, 7.16%, 3.13%, and 1.56 against the bacteria Propionibacterium acnes and Staphylococcus aureus. The combination of dry extract of aloe vera and thick red betel leaf extract have antibacterial activity against Staphylococcus aureus bacteria at concentration 1.56% and Propionibacterium acne at concentration 3,13 %. Keywords: aloe vera, red betel leaf extract, Propionibacterium acnes, Staphylococcus aureus

scholarly journals Analysis of The Phytochemical Characteristics of Jasmine Flower Against Escherichia coli and Staphylococcus aureus

2021 ◽  
Vol 15 (3) ◽  
pp. 4
Author(s):  
Nathasa Sihite
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Ethyl Acetate ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Agricultural Product ◽  
Disc Diffusion Method ◽  
Water Solvent ◽  
Jasminum Sambac ◽  
And Staphylococcus Aureus

Indonesia is a famous country had the diversity of plants, especially agricultural product and herbs, they had a potential of antimicrobial as a like Jasmine Flower (Jasminum sambac Ait). This study Indonesia is a famous country had the diversity of plants, especially agricultural product and herbs, they had a potential of antimicrobial as a like Jasmine Flower (Jasminum sambac Ait). This study aimed to determine the phytochemical and antimicrobial activity of jasmine flower. Extraction was done by maceration, such as using water solvent, methanol, ethyl acetate, and hexane as the solvent. The sample bacteria used were Escherichia coli and Staphylococcus aureus. This study used disc diffusion method, with a complete 2-factor random design and 4 replications. Processing of the data is using ANOVA. The results showed that the jasmine extracts with a concentration of 25%, 50%, 75% and 100% had an effect to inhibit of the growth of the bacteria. Jasmine extract with ethyl acetate solvent was the most effective to inhibit the growth of bacteria. It was known that jasmine flower extract is one of the good natural antimicrobials gave an inhibitory effect to the growth of Escherichia coli and Staphylococcus aureus bacteria. Keywords: jasmine, Escherichia coli, Sthaphylococcus aureus, antimicrobials

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