scholarly journals Finding acceptable parameter regions of stochastic Hill functions for multisite phosphorylation mechanism

2020 ◽  
Vol 152 (12) ◽  
pp. 124108
Author(s):  
M. Chen ◽  
M. Ahmadian ◽  
L. T. Watson ◽  
Y. Cao
Keyword(s):  
Multisite Phosphorylation ◽  
Phosphorylation Mechanism ◽  
Acceptable Parameter

scholarly journals A novel phospho-modulatory mechanism contributes to the calcium-dependent regulation of T-type Ca2+ channels

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Jean Chemin ◽  
Tamara Timic Stamenic ◽  
Magalie Cazade ◽  
Jodie Llinares ◽  
Iulia Blesneac ◽  
...  
Keyword(s):  
Calcium Dependent ◽  
Cell Functions ◽  
Medial Nucleus ◽  
Steady State Inactivation ◽  
Intracellular Ca ◽  
Phosphorylation Mechanism ◽  
Multiple Cell ◽  
Atp Analogs ◽  
Ca2 Channels ◽  
Endocytic Pathways

Abstract Cav3 / T-type Ca2+ channels are dynamically regulated by intracellular Ca2+ ions, which inhibit Cav3 availability. Here, we demonstrate that this inhibition becomes irreversible in the presence of non-hydrolysable ATP analogs, resulting in a strong hyperpolarizing shift in the steady-state inactivation of the residual Cav3 current. Importantly, the effect of these ATP analogs was prevented in the presence of intracellular BAPTA. Additional findings obtained using intracellular dialysis of inorganic phosphate and alkaline phosphatase or NaN3 treatment further support the involvement of a phosphorylation mechanism. Contrasting with Cav1 and Cav2 Ca2+ channels, the Ca2+-dependent modulation of Cav3 channels appears to be independent of calmodulin, calcineurin and endocytic pathways. Similar findings were obtained for the native T-type Ca2+ current recorded in rat thalamic neurons of the central medial nucleus. Overall, our data reveal a new Ca2+ sensitive phosphorylation-dependent mechanism regulating Cav3 channels, with potentially important physiological implications for the multiple cell functions controlled by T-type Ca2+ channels.

scholarly journals Multisite Phosphorylation of Nuclear Interaction Partner of ALK (NIPA) at G2/M Involves Cyclin B1/Cdk1

2007 ◽  
Vol 282 (22) ◽  
pp. 15965-15972 ◽  
Author(s):  
Florian Bassermann ◽  
Christine von Klitzing ◽  
Anna Lena Illert ◽  
Silvia Münch ◽  
Stephan W. Morris ◽  
...  
Keyword(s):  
Nuclear Interaction ◽  
Cyclin B1 ◽  
Interaction Partner ◽  
Multisite Phosphorylation

scholarly journals Autoinhibition and Polo-Dependent Multisite Phosphorylation Restrict Activity of the Histone H3 Kinase Haspin to Mitosis

2013 ◽  
Vol 52 (5) ◽  
pp. 734-745 ◽  
Author(s):  
Cristina Ghenoiu ◽  
Michael S. Wheelock ◽  
Hironori Funabiki
Keyword(s):  
Histone H3 ◽  
Multisite Phosphorylation
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