Kinetic study of mixed glucose and fructose fermentation using Saccharomyces cerevisiae with the presence of Ca2+ ion

2020 ◽  
Author(s):  
H. Hargono ◽  
Bakti Jos ◽  
Teguh Riyanto ◽  
Dhiya’ Acfira Tsaniatri
2007 ◽  
Vol 73 (8) ◽  
pp. 2432-2439 ◽  
Author(s):  
Carole Guillaume ◽  
Pierre Delobel ◽  
Jean-Marie Sablayrolles ◽  
Bruno Blondin

ABSTRACT Fructose utilization by wine yeasts is critically important for the maintenance of a high fermentation rate at the end of alcoholic fermentation. A Saccharomyces cerevisiae wine yeast able to ferment grape must sugars to dryness was found to have a high fructose utilization capacity. We investigated the molecular basis of this enhanced fructose utilization capacity by studying the properties of several hexose transporter (HXT) genes. We found that this wine yeast harbored a mutated HXT3 allele. A functional analysis of this mutated allele was performed by examining expression in an hxt1-7Δ strain. Expression of the mutated allele alone was found to be sufficient for producing an increase in fructose utilization during fermentation similar to that observed in the commercial wine yeast. This work provides the first demonstration that the pattern of fructose utilization during wine fermentation can be altered by expression of a mutated hexose transporter in a wine yeast. We also found that the glycolytic flux could be increased by overexpression of the mutant transporter gene, with no effect on fructose utilization. Our data demonstrate that the Hxt3 hexose transporter plays a key role in determining the glucose/fructose utilization ratio during fermentation.


2010 ◽  
Vol 85 (10) ◽  
pp. 1361-1367 ◽  
Author(s):  
Benigno Ortiz-Muñiz ◽  
Octavio Carvajal-Zarrabal ◽  
Beatriz Torrestiana-Sanchez ◽  
Maria Guadalupe Aguilar-Uscanga

2014 ◽  
Vol 04 (01) ◽  
pp. 26-31 ◽  
Author(s):  
Egharevba Felix ◽  
Ogbebor Clara ◽  
Akpoveta Oshevwiyo Vincent

1989 ◽  
Vol 42 (1) ◽  
pp. B1-B7 ◽  
Author(s):  
Isidoro García García ◽  
Antonio Martín Martín ◽  
José María Ortega Ruiz ◽  
Arturo Chica Pérez

2010 ◽  
Vol 45 (4) ◽  
pp. 493-499 ◽  
Author(s):  
Patricio Gutiérrez ◽  
Ana Roldán ◽  
Ildefonso Caro ◽  
Luis Pérez

Sign in / Sign up

Export Citation Format

Share Document