Production of biodiesel from the locally isolated yellow strain of Chlorella sp. using dairy wastewater as a growth medium

2019 ◽  
Author(s):  
Djillali Ghobrini ◽  
Tomáš Brányik ◽  
Saliha Yakoub-Bougdal ◽  
Kamal Aïboud ◽  
Leïla Kebbab ◽  
...  
Keyword(s):  
Growth Medium ◽  
Dairy Wastewater ◽  
Chlorella Sp ◽  
Yellow Strain

scholarly journals 244 Algae taste preference of Chlorella sp. from dairy wastewater by weaned dairy calves

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 15-15
Author(s):  
Bradley J Heins ◽  
Siane C Luzzi ◽  
Robert D Gardner
Keyword(s):  
Dry Matter ◽  
Taste Preference ◽  
Dairy Wastewater ◽  
Control Treatment ◽  
Central Research ◽  
Solar Panels ◽  
University Of Minnesota ◽  
Chlorella Sp ◽  
Coefficient Of Concordance ◽  
The Difference

Abstract The objective of this study was to evaluate the taste preference of calves fed Chlorella sp. microalgae produced from dairy lagoon wastewater. The study was conducted at the University of Minnesota West Central Research and Outreach Center, Morris, MN, dairy during May 2019. Six Holstein and crossbred dairy heifer calves were fed 0 (control), 30, and 60 g of Chlorella sp. daily in a sequential elimination study. For the 7-d experiment, day 1 to 2 were for diet adaptation and day 3 to 4 were for data collection. During the final 3 days, the primarily consumed treatment was removed to determine the second preferred treatment. The microalgae used in this study was isolated from the dairy wastewater lagoon. The microalgae biomass was produced using outdoor hanging bag bioreactors with Chlorella sp. to recycle the dairy wastewater. The biomass was sterilized and kept frozen at -4°C until fed to calves. Calves were housed individually in hutches with outdoor access under solar panels, with free-choice water. Kendall’s coefficient of concordance was calculated to rank the consumption of the treatments from most to least preferred using JMP 14.3 statistical software. Pairwise comparisons and Tukey adjustment were applied to evaluate the difference between the treatments for total intake. Calves consumed more (P < 0.05) dry matter from control grain (3.4 kg/d) compared to 30 g microalgae grain (2.42 kg/d) and 60 g microalgae grain (1.56 kg/d) during the first 2-d period. During the second 2-d (d 3 and 4) segment, dry matter intake was reduced (P < 0.05) for the 60 g microalgae treatment compared to the control and 30 g microalgae treatment. Five of six calves in this study always ranked the control treatment first (P < 0.05) when given a choice, and ranked the 30 g microalgae second choice. Results indicated that microalgae maybe added to calf starter grains without any adverse effects; however, calves preferred calf starter grains without microalgae.

scholarly journals Potential of Microalgae Cultivation in Dairy Wastewater as a Step in Low-Cost Biofuel Production

2018 ◽  
Vol 24 (4) ◽  
pp. 58
Author(s):  
Basma Abbas Abdulmajeed ◽  
Arwa Raad Ibrahim
Keyword(s):  
Wastewater Treatment ◽  
Renewable Energy ◽  
Chemical Oxygen Demand ◽  
Low Cost ◽  
Oxygen Demand ◽  
Dairy Wastewater ◽  
Biofuel Production ◽  
Microalgae Cultivation ◽  
Chlorella Sp

The present study addresses adopting the organic and nutritious materials in dairy wastewater as media for cultivation of microalgae, which represent an important source of renewable energy. This study was carried out through cultivation of three types of microalgae; Chlorella sp., Synechococcus, and Anabaena. The results shows the success the cultivation of the Synechococcus and  Chlorella Sp, while the Anabaena microalgae were in low-growth level. The highest growth was in the Synechococcus farm, followed by Chlorella and Anabaena. However, the growth of Synechococcus required 10 days to achieve this increase that represents a negative indicator of the adoption of this type of microalgae in this media to meet the desired aims. While Chlorella needs less than two days to start growing. Moreover, the data obtained from the experiment show that removal of chemical oxygen demand in Chlorella cultures was (72%) more than that obtained from cultivation of other microalgae. Thus this microalgae is more efficient in wastewater treatment than other types.  

Valorization of residual soft drinks by baker's yeast production and insight for dairy wastewater whey incorporation

2019 ◽  
Vol 79 (4) ◽  
pp. 635-644
Author(s):  
Mariam Kasmi ◽  
Amjad Kallel ◽  
Lobna Elleuch ◽  
Moktar Hamdi ◽  
Ismail Trabelsi
Keyword(s):  
Growth Medium ◽  
Soft Drink ◽  
Soft Drinks ◽  
Baker’S Yeast ◽  
Dairy Wastewater ◽  
Partial Substitution ◽  
Growth Media ◽  
Baker's Yeast ◽  
Conversion Rates ◽  
Yeast Production

Abstract Residuals are responsible for the polluting load increase of soft drink industry wastewater due to their high sugar contents. The present work proposes an upstream segregation of residuals to be biologically treated by the bioconversion of their carbohydrates content into baker's yeast biomass. Carbonated soft drinks (CSD) and nectars and juices (NJ) ranges were considered. Different incorporation ratios of NJ in the CSD (0–75%) have been investigated for balanced growth medium. Despite the nitrogen deficiency of media, results showed that NJ incorporation promoted the microbial growth. Media containing more than 50% of NJ exhibited ∼25% sugar-biomass conversion rates. The chemical oxygen demand (COD) of the media exceeded 70% at the end of fermentation. Moreover, valuable components were recovered by yeast production. Nutrient consumption rates varied from 65.4% for sugar and calcium content to in excess of 99% for protein and other minerals. In order to investigate an available and low-cost source of nitrogen for yeast production, partial substitution of the soft drink growth medium by bactofugate whey was evaluated. The soft drink-whey mixture medium fermentation resulted in 63% COD removal rate after 28 h. Meanwhile, the biomass production yield revealed an improvement of about 25% compared to the balanced soft drink medium (NJ50).

scholarly journals Taste preference of Chlorella sp. algae from dairy wastewater by weaned dairy calves

2020 ◽  
Vol 1 (2) ◽  
pp. 41-44
Author(s):  
S.C. Luzzi ◽  
R.D. Gardner ◽  
B.J. Heins
Keyword(s):  
Taste Preference ◽  
Dairy Wastewater ◽  
Dairy Calves ◽  
Chlorella Sp

scholarly journals THE USE OF DAIRY WASTEWATER AS A MEDIUM FOR CHLORELLA SP.

2017 ◽  
Vol 18 (5) ◽  
pp. 5-9
Author(s):  
Karolina Dziosa ◽  
Monika Makowska
Keyword(s):  
Dairy Wastewater ◽  
Chlorella Sp

Ultrastructural Alterations in Hela Cells Induced by Spider Venom

1967 ◽  
Vol 25 ◽  
pp. 20-21
Author(s):  
Dale E. McClendon ◽  
Paul N. Morgan ◽  
Bernard L. Soloff
Keyword(s):  
Growth Medium ◽  
Mammalian Cells ◽  
Carcinoma Cells ◽  
Venom Protein ◽  
Sterile Saline ◽  
Carbon Coated ◽  
Available Information ◽  
Loxosceles Reclusa ◽  
Essential Growth ◽  
Solution Cultures

It has been observed that minute amounts of venom from the brown recluse spider, Loxosceles reclusa, are capable of producing cytotoxic changes in cultures of certain mammalian cells (Morgan and Felton, 1965). Since there is little available information concerning the effect of venoms on susceptible cells, we have attempted to characterize, at the electron microscope level, the cytotoxic changes produced by the venom of this spider.Cultures of human epithelial carcinoma cells, strain HeLa, were initiated on sterile, carbon coated coverslips contained in Leighton tubes. Each culture was seeded with approximately 1x105 cells contained in 1.5 ml of a modified Eagle's minimum essential growth medium prepared in Hank's balanced salt solution. Cultures were incubated at 36° C. for three days prior to the addition of venom. The venom was collected from female brown recluse spiders and diluted in sterile saline. Protein determinations on the venom-were made according to the spectrophotometric method of Waddell (1956). Approximately 10 μg venom protein per ml of fresh medium was added to each culture after discarding the old growth medium. Control cultures were treated similarly, except that no venom was added. All cultures were reincubated at 36° C.

Effect of Iron Limitation on the Ultrastructure of Agmenellum Quadruplicatum

1981 ◽  
Vol 39 ◽  
pp. 410-411
Author(s):  
L. P. Hardie ◽  
D. L. Balkwill ◽  
S. E. Stevens
Keyword(s):  
Growth Medium ◽  
Green Alga ◽  
Natural Habitat ◽  
Iron Limitation ◽  
Natural Environments ◽  
Blue Green Alga ◽  
Marine Cyanobacterium ◽  
Natural Systems ◽  
Thin Sectioning ◽  
Agmenellum Quadruplicatum

Agmenellum quadruplicatum is a unicellular, non-nitrogen-fixing, marine cyanobacterium (blue-green alga). The ultrastructure of this organism, when grown in the laboratory with all necessary nutrients, has been characterized thoroughly. In contrast, little is known of its ultrastructure in the specific nutrient-limiting conditions typical of its natural habitat. Iron is one of the nutrients likely to limit this organism in such natural environments. It is also of great importance metabolically, being required for both photosynthesis and assimilation of nitrate. The purpose of this study was to assess the effects (if any) of iron limitation on the ultrastructure of A. quadruplicatum. It was part of a broader endeavor to elucidate the ultrastructure of cyanobacteria in natural systemsActively growing cells were placed in a growth medium containing 1% of its usual iron. The cultures were then sampled periodically for 10 days and prepared for thin sectioning TEM to assess the effects of iron limitation.

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