scholarly journals A simple and rapid detection system for oral bacteria in liquid phase for point-of-care diagnostics using magnetic nanoparticles

AIP Advances ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 125325
Author(s):  
Loi Tonthat ◽  
Shunnosuke Takahashi ◽  
Hidehiko Onodera ◽  
Kazuhiko Okita ◽  
Shin Yabukami ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Liquid Phase ◽  
Rapid Detection ◽  
Detection System ◽  
Point Of Care ◽  
Oral Bacteria ◽  
Point Of Care Diagnostics

Polyelectrolyte-Coated Gold Magnetic Nanoparticles for Immunoassay Development: Toward Point of Care Diagnostics for Syphilis Screening

2013 ◽  
Vol 85 (14) ◽  
pp. 6688-6695 ◽  
Author(s):  
Dong Yang ◽  
Jianzhong Ma ◽  
Qinlu Zhang ◽  
Ningning Li ◽  
Jiangcun Yang ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Point Of Care ◽  
Point Of Care Diagnostics ◽  
Syphilis Screening

Multi-Channel Immunoassay System with Electrochemical Detection Toward Point-of-Care Diagnostics Using Magnetic Nanoparticles

2020 ◽  
Vol MA2020-01 (27) ◽  
pp. 1925-1925
Author(s):  
Sunga Song ◽  
Young Joo Kim ◽  
Hye-Lim Kang ◽  
Sumi Yoon ◽  
Dong-ki Hong ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Electrochemical Detection ◽  
Point Of Care ◽  
Point Of Care Diagnostics

scholarly journals 2019 Novel Coronavirus Disease (COVID-19): Paving the Road for Rapid Detection and Point-of-Care Diagnostics

Micromachines ◽  
2020 ◽  
Vol 11 (3) ◽  
pp. 306 ◽  
Author(s):  
Trieu Nguyen ◽  
Dang Duong Bang ◽  
Anders Wolff
Keyword(s):  
Rapid Detection ◽  
Point Of Care ◽  
Potential Candidate ◽  
The Road ◽  
Point Of Care Diagnostics ◽  
Novel Coronavirus

We believe a point-of-care (PoC) device for the rapid detection of the 2019 novel Coronavirus (SARS-CoV-2) is crucial and urgently needed. With this perspective, we give suggestions regarding a potential candidate for the rapid detection of the coronavirus disease 2019 (COVID-19), as well as factors for the preparedness and response to the outbreak of the COVID-19.

Opto-fluidic detection system enabling sophisticated point-of-care diagnostics

2011 ◽  
Author(s):  
Peter Kiesel ◽  
Markus Beck ◽  
Joerg Martini ◽  
Noble Johnson ◽  
Malte Huck
Keyword(s):  
Detection System ◽  
Point Of Care ◽  
Point Of Care Diagnostics

scholarly journals Highly Specific and Sensitive Detection of Yersinia pestis by Portable Cas12a-UPTLFA Platform

2021 ◽  
Vol 12 ◽  
Author(s):  
Yang You ◽  
Pingping Zhang ◽  
Gengshan Wu ◽  
Yafang Tan ◽  
Yong Zhao ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Yersinia Pestis ◽  
Rapid Detection ◽  
Detection Method ◽  
Detection System ◽  
Point Of Care ◽  
High Sensitivity ◽  
Nucleic Acid Detection ◽  
Great Promise ◽  
High Background

The recent discovery of collateral cleavage activity of class-II clustered regularly interspaced short palindromic repeats–CRISPR-associated protein (CRISPR-Cas) makes CRISPR-based diagnosis a potential high-accuracy nucleic acid detection method. Colloidal gold-based lateral flow immunochromatographic assay (LFA), which has been combined with CRISPR/Cas-based nucleic detection, usually associates with drawbacks of relative high background and the subjectivity in naked-eye read-out of the results. Here, we developed a novel system composed of Cas12a-based nucleic acid detection and up-converting phosphor technology (UPT)-based LFA (UPT–LFA), termed Cas12a-UPTLFA. We further demonstrated the utility of this platform in highly sensitive and specific detection of Yersinia pestis, the causative agent of the deadly plague. Due to high infectivity and mortality, as well as the potential to be misused as bioterrorism agent, a culture-free, ultrasensitive, specific, and rapid detection method for Y. pestis has long been desired. By incorporating isothermal recombinase polymerase amplification, the Cas12a-UPTLFA we established can successfully detect genomic DNA of Y. pestis as low as 3 attomolar (aM) and exhibited high sensitivity (93.75%) and specificity (90.63%) for detection of spiked blood samples with a detection limit of 102 colony-forming unit per 100 μl of mouse blood. With a portable biosensor, Cas12a-UPTLFA assay can be operated easily by non-professional personnel. Taken together, we have developed a novel Cas12a-UPTLFA platform for rapid detection of Y. pestis with high sensitivity and specificity, which is portable, not expensive, and easy to operate as a point-of-care method. This detection system can easily be extended to detect other pathogens and holds great promise for on-site detection of emerging infectious pathogens.

scholarly journals Contribution of Nanotechnology in the Fight Against COVID-19

2020 ◽  
Vol 11 (1) ◽  
pp. 8233-8241 ◽  
Keyword(s):  
Rapid Detection ◽  
Vaccine Development ◽  
Point Of Care ◽  
Advanced Technology ◽  
Development Research ◽  
New Materials ◽  
The Earth ◽  
Point Of Care Diagnostics ◽  
Funding Agencies ◽  
New Research

Coronavirus disease (COVID-19) is a respiratory infectious disease caused by a newly discovered virus strain, severe acute respiratory syndrome coronavirus-2 (SARS-Cov-2). This pandemic spread quickly across nations with a high mortality rate in immunocompromised patients. This contagious disease posed a serious threat to health systems. It impacted the continents of the earth in a way that could not have been predicted. Therefore, many leading funding agencies announced the call for proposal to diagnosis and treatment of COVID-19 pandemic using advanced technology-based methods, including nanotechnology. The researchers coming from the nanotechnology community can contribute their efforts to cope with COVID-19. As a community member of nanotechnology, we suggest some new research targets that can be designed and improved, optimized, and developed the existing/new materials in the sub-field of diagnostics and healthcare of nanotechnology. The potential research targets to fight against COVID-19 includes Point-of-care diagnostics (POCD), surveillance and monitoring, novel therapeutics, vaccine development, research, and development, repurposing existing drugs with potential therapeutic applications, development of antiviral nanocoating/antimicrobial spray-based coating for PPE, magnetic nanoparticles and viral RNA and rapid detection kits.

scholarly journals Functionalized TiO2 Nanotube-Based Electrochemical Biosensor for Rapid Detection of SARS-CoV-2

Sensors ◽  
2020 ◽  
Vol 20 (20) ◽  
pp. 5871 ◽  
Author(s):  
Bhaskar S. Vadlamani ◽  
Timsy Uppal ◽  
Subhash C. Verma ◽  
Mano Misra
Keyword(s):  
Rapid Detection ◽  
Point Of Care ◽  
Low Cost ◽  
Viral Disease ◽  
Electrochemical Anodization ◽  
Prophylactic Measure ◽  
Point Of Care Diagnostics ◽  
First Case ◽  
One Step ◽  
Asymptomatic Individuals

The COronaVIrus Disease (COVID-19) is a newly emerging viral disease caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Rapid increase in the number of COVID-19 cases worldwide led the WHO to declare a pandemic within a few months after the first case of infection. Due to the lack of a prophylactic measure to control the virus infection and spread, early diagnosis and quarantining of infected as well as the asymptomatic individuals are necessary for the containment of this pandemic. However, the current methods for SARS-CoV-2 diagnosis are expensive and time consuming, although some promising and inexpensive technologies are becoming available for emergency use. In this work, we report the synthesis of a cheap, yet highly sensitive, cobalt-functionalized TiO2 nanotubes (Co-TNTs)-based electrochemical sensor for rapid detection of SARS-CoV-2 through sensing the spike (receptor binding domain (RBD)) present on the surface of the virus. A simple, low-cost, and one-step electrochemical anodization route was used for synthesizing TNTs, followed by an incipient wetting method for cobalt functionalization of the TNTs platform, which was connected to a potentiostat for data collection. This sensor specifically detected the S-RBD protein of SARS-CoV-2 even at very low concentration (range of 14 to 1400 nM (nano molar)). Additionally, our sensor showed a linear response in the detection of viral protein over the concentration range. Thus, our Co-TNT sensor is highly effective in detecting SARS-CoV-2 S-RBD protein in approximately 30 s, which can be explored for developing a point of care diagnostics for rapid detection of SARS-CoV-2 in nasal secretions and saliva samples.

Identification of hepatitis a virus in cell cultures by solid phase immune electron microscopy

1986 ◽  
Vol 44 ◽  
pp. 238-239
Author(s):  
C.D. Humphrey ◽  
T.L. Cromeans ◽  
E.H. Cook ◽  
D.W. Bradley
Keyword(s):  
Electron Microscopy ◽  
Liquid Phase ◽  
Cell Cultures ◽  
Rapid Detection ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Solid Phase ◽  
Immune Electron Microscopy ◽  
Detection And Identification

There is a variety of methods available for the rapid detection and identification of viruses by electron microscopy as described in several reviews. The predominant techniques are classified as direct electron microscopy (DEM), immune electron microscopy (IEM), liquid phase immune electron microscopy (LPIEM) and solid phase immune electron microscopy (SPIEM). Each technique has inherent strengths and weaknesses. However, in recent years, the most progress for identifying viruses has been realized by the utilization of SPIEM.

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