A simple method for in situ measurement of vacuum window birefringence

2019 ◽  
Vol 90 (11) ◽  
pp. 113001
Author(s):  
W. H. Yuan ◽  
H. L. Liu ◽  
W. Z. Wei ◽  
Z. Y. Ma ◽  
P. Hao ◽  
...  
2013 ◽  
Vol 84 (12) ◽  
pp. 126103 ◽  
Author(s):  
Andreas Steffen ◽  
Wolfgang Alt ◽  
Maximilian Genske ◽  
Dieter Meschede ◽  
Carsten Robens ◽  
...  

Author(s):  
W. H. Yuan ◽  
H. L. Liu ◽  
W. Z. Wei ◽  
Z. Y. Ma ◽  
P. Hao ◽  
...  

Author(s):  
Dean A. Handley ◽  
Jack T. Alexander ◽  
Shu Chien

In situ preparation of cell cultures for ultrastructural investigations is a convenient method by which fixation, dehydration and embedment are carried out in the culture petri dish. The in situ method offers the advantage of preserving the native orientation of cell-cell interactions, junctional regions and overlapping configurations. In order to section after embedment, the petri dish is usually separated from the polymerized resin by either differential cryo-contraction or solvation in organic fluids. The remaining resin block must be re-embedded before sectioning. Although removal of the petri dish may not disrupt the native cellular geometry, it does sacrifice what is now recognized as an important characteristic of cell growth: cell-substratum molecular interactions. To preserve the topographic cell-substratum relationship, we developed a simple method of tapered rotary beveling to reduce the petri dish thickness to a dimension suitable for direct thin sectioning.


2020 ◽  
Author(s):  
Aidan Kelly ◽  
Peng-Jui (Ruby) Chen ◽  
Jenna Klubnick ◽  
Daniel J. Blair ◽  
Martin D. Burke

<div> <div> <div> <p>Existing methods for making MIDA boronates require harsh conditions and complex procedures to achieve dehydration. Here we disclose that a pre-dried form of MIDA, MIDA anhydride, acts as both a source of the MIDA ligand and an in situ desiccant to enable a mild and simple MIDA boronate synthesis procedure. This method expands the range of sensitive boronic acids that can be converted into their MIDA boronate counterparts. Further utilizing unique properties of MIDA boronates, we have developed a MIDA Boronate Maker Kit which enables the direct preparation and purification of MIDA boronates from boronic acids using only heating and centrifuge equipment that is widely available in labs that do not specialize in organic synthesis. </p> </div> </div> </div>


2003 ◽  
Vol 2 (4) ◽  
pp. 589
Author(s):  
Douglas R. Cobos ◽  
John M. Baker

2015 ◽  
Vol 84 (8) ◽  
pp. 567-572
Author(s):  
Tadafumi HASHIMOTO ◽  
Masahito MOCHIZUKI

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