Investigation of the effect of substrate morphology on MDCK cell mechanical behavior using atomic force microscopy

2019 ◽  
Vol 115 (6) ◽  
pp. 063701 ◽  
Author(s):  
Keyvan Mollaeian ◽  
Yi Liu ◽  
Siyu Bi ◽  
Juan Ren
Keyword(s):  
Atomic Force Microscopy ◽  
Mechanical Behavior ◽  
Mdck Cell ◽  
Force Microscopy ◽  
Atomic Force

Liquid Mechanical Behavior of Mixed Monolayers of Amino and Alkyl Silanes by Atomic Force Microscopy

Langmuir ◽  
2005 ◽  
Vol 21 (15) ◽  
pp. 6934-6943 ◽  
Author(s):  
Pascal Martin ◽  
Sophie Marsaudon ◽  
Laurent Thomas ◽  
Bernard Desbat ◽  
Jean-Pierre Aimé ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Mechanical Behavior ◽  
Mixed Monolayers ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Multiscale Network Modeling of Fibrin Fibers and Fibrin Clots with Protofibril Binding Mechanics

Polymers ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 1223
Author(s):  
Sumith Yesudasan ◽  
Rodney D. Averett
Keyword(s):  
Atomic Force Microscopy ◽  
Mechanical Behavior ◽  
Network Model ◽  
Large Strains ◽  
Chain Model ◽  
Force Extension ◽  
Nonlinear Spring ◽  
Force Microscopy ◽  
Atomic Force ◽  
Fibrin Clots

The multiscale mechanical behavior of individual fibrin fibers and fibrin clots was modeled by coupling atomistic simulation data and microscopic experimental data. We propose a new protofibril element composed of a nonlinear spring network, and constructed this based on molecular simulations and atomic force microscopy results to simulate the force extension behavior of fibrin fibers. This new network model also accounts for the complex interaction of protofibrils with one another, the effects of the presence of a solvent, Coulombic attraction, and other binding forces. The network model was formulated to simulate the force–extension mechanical behavior of single fibrin fibers from atomic force microscopy experiments, and shows good agreement. The validated fibrin fiber network model was then combined with a modified version of the Arruda–Boyce eight-chain model to estimate the force extension behavior of the fibrin clot at the continuum level, which shows very good correlation. The results show that our network model is able to predict the behavior of fibrin fibers as well as fibrin clots at small strains, large strains, and close to the break strain. We used the network model to explain why the mechanical response of fibrin clots and fibrin fibers deviates from worm-like chain behavior, and instead behaves like a nonlinear spring.

Measurement of the mechanical behavior of yeast membrane sensors using single-molecule atomic force microscopy

2010 ◽  
Vol 5 (4) ◽  
pp. 670-677 ◽  
Author(s):  
Jürgen J Heinisch ◽  
Vincent Dupres ◽  
David Alsteens ◽  
Yves F Dufrêne
Keyword(s):  
Atomic Force Microscopy ◽  
Mechanical Behavior ◽  
Single Molecule ◽  
Force Microscopy ◽  
Atomic Force

Entropic Effects on the Mechanical Behavior of Dry Polymer Brushes During Nanoindentation by Atomic Force Microscopy

2011 ◽  
Vol 44 (2) ◽  
pp. 368-374 ◽  
Author(s):  
Davide Tranchida ◽  
Elena Sperotto ◽  
Antoine Chateauminois ◽  
Holger Schönherr
Keyword(s):  
Atomic Force Microscopy ◽  
Mechanical Behavior ◽  
Polymer Brushes ◽  
Force Microscopy ◽  
Atomic Force ◽  
Entropic Effects

Determination of Strain-Rate-Dependent Mechanical Behavior of Living and Fixed Osteocytes and Chondrocytes Using Atomic Force Microscopy and Inverse Finite Element Analysis

2014 ◽  
Vol 136 (10) ◽  
Author(s):  
Trung Dung Nguyen ◽  
YuanTong Gu
Keyword(s):  
Finite Element Analysis ◽  
Atomic Force Microscopy ◽  
Strain Rate ◽  
Mechanical Behavior ◽  
Strain Rates ◽  
Element Analysis ◽  
Force Microscopy ◽  
Rate Dependent ◽  
Atomic Force ◽  
Inverse Finite Element Analysis

The aim of this paper is to determine the strain-rate-dependent mechanical behavior of living and fixed osteocytes and chondrocytes, in vitro. First, atomic force microscopy (AFM) was used to obtain the force–indentation curves of these single cells at four different strain-rates. These results were then employed in inverse finite element analysis (FEA) using modified standard neo-Hookean solid (MSnHS) idealization of these cells to determine their mechanical properties. In addition, a FEA model with a newly developed spring element was employed to accurately simulate AFM evaluation in this study. We report that both cytoskeleton (CSK) and intracellular fluid govern the strain-rate-dependent mechanical property of living cells whereas intracellular fluid plays a predominant role on fixed cells' behavior. In addition, through the comparisons, it can be concluded that osteocytes are stiffer than chondrocytes at all strain-rates tested indicating that the cells could be the biomarker of their tissue origin. Finally, we report that MSnHS is able to capture the strain-rate-dependent mechanical behavior of osteocyte and chondrocyte for both living and fixed cells. Therefore, we concluded that the MSnHS is a good model for exploration of mechanical deformation responses of single osteocytes and chondrocytes. This study could open a new avenue for analysis of mechanical behavior of osteocytes and chondrocytes as well as other similar types of cells.

scholarly journals Mechanical Properties of Boehmite Evaluated by Atomic Force Microscopy Experiments and Molecular Dynamic Finite Element Simulations

2016 ◽  
Vol 2016 ◽  
pp. 1-13 ◽  
Author(s):  
J. Fankhänel ◽  
D. Silbernagl ◽  
M. Ghasem Zadeh Khorasani ◽  
B. Daum ◽  
A. Kempe ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Atomic Force Microscopy ◽  
Finite Element ◽  
Mechanical Behavior ◽  
Molecular Dynamic ◽  
Fiber Reinforced Polymers ◽  
Force Microscopy ◽  
Dynamic Finite Element ◽  
Atomic Force ◽  
Boehmite Nanoparticles

Boehmite nanoparticles show great potential in improving mechanical properties of fiber reinforced polymers. In order to predict the properties of nanocomposites, knowledge about the material parameters of the constituent phases, including the boehmite particles, is crucial. In this study, the mechanical behavior of boehmite is investigated using Atomic Force Microscopy (AFM) experiments and Molecular Dynamic Finite Element Method (MDFEM) simulations. Young’s modulus of the perfect crystalline boehmite nanoparticles is derived from numerical AFM simulations. Results of AFM experiments on boehmite nanoparticles deviate significantly. Possible causes are identified by experiments on complementary types of boehmite, that is, geological and hydrothermally synthesized samples, and further simulations of imperfect crystals and combined boehmite/epoxy models. Under certain circumstances, the mechanical behavior of boehmite was found to be dominated by inelastic effects that are discussed in detail in the present work. The studies are substantiated with accompanying X-ray diffraction and Raman experiments.

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