scholarly journals On the transport of particles/cells in high-throughput deterministic lateral displacement devices: Implications for circulating tumor cell separation

2019 ◽  
Vol 13 (3) ◽  
pp. 034112 ◽  
Author(s):  
Arian Aghilinejad ◽  
Mohammad Aghaamoo ◽  
Xiaolin Chen
Keyword(s):  
Tumor Cell ◽  
High Throughput ◽  
Cell Separation ◽  
Lateral Displacement ◽  
Circulating Tumor Cell ◽  
Deterministic Lateral Displacement

Study of Angle-of-Attack (AoA) for Airfoil in Deterministic Lateral Displacement (DLD)

2019 ◽  
Author(s):  
Kawkab Ahasan ◽  
Jong-Hoon Kim
Keyword(s):  
High Throughput ◽  
Lateral Displacement ◽  
Angle Of Attack ◽  
Critical Diameter ◽  
Control Flow ◽  
Flow Rates ◽  
Deterministic Lateral Displacement ◽  
Potential Applications ◽  
High Reynolds ◽  
Flow Symmetry

Abstract Deterministic lateral displacement (DLD) is a method of inertial size-based particle separation with potential applications in high throughput sample processing, such as the fractionation of blood or the purification of target species like viral particles or circulating tumor cells. Recently, it has been shown that symmetric airfoils with neutral angle-of-attack (AoA) can be used for high-throughput design of DLD device, due to their mitigation of vortex effects and preservation of flow symmetry under high Reynolds number (Re) conditions. While high-Re operation with symmetric airfoils has been established, the effect of AoA for airfoil on the DLD performance has not been characterized. In this study, we present a high-Re investigation with symmetric airfoil-shaped pillars having positive and negative 15 degree AoA. Both positive and negative AoA configurations yield significant flow anisotropy at higher flow rates. The stronger shift of the critical diameter (Dc) was observed with negative AoA, but not in positive AoA device. The most likely contributor may be the growing anisotropy that develops in the AoA device at higher flow rates. This study shows that high-Re DLD design with airfoil shaped pillars requires significant consideration for pillar orientation to control flow symmetry.

scholarly journals Circulating Tumor Cell Phenotyping via High‐Throughput Acoustic Separation

Small ◽  
2020 ◽  
Vol 16 (34) ◽  
pp. 2004438
Author(s):  
Mengxi Wu ◽  
Po‐Hsun Huang ◽  
Rui Zhang ◽  
Zhangming Mao ◽  
Chuyi Chen ◽  
...  
Keyword(s):  
Tumor Cell ◽  
High Throughput ◽  
Circulating Tumor Cell ◽  
Acoustic Separation

scholarly journals Sheathless High-Throughput Circulating Tumor Cell Separation Using Viscoelastic non-Newtonian Fluid

Micromachines ◽  
2019 ◽  
Vol 10 (7) ◽  
pp. 462 ◽  
Author(s):  
Hyunjung Lim ◽  
Seung Min Back ◽  
Min Ho Hwang ◽  
Dae-Hee Lee ◽  
Hyuk Choi ◽  
...  
Keyword(s):  
High Throughput ◽  
Blood Cells ◽  
Cell Separation ◽  
Separation Efficiency ◽  
White Blood Cells ◽  
Flow Characteristics ◽  
Circulating Tumor Cell ◽  
Size Difference ◽  
Biological Applications ◽  
Mcf 7

Circulating tumor cells (CTCs) have attracted increasing attention as important biomarkers for clinical and biological applications. Several microfluidic approaches have been demonstrated to separate CTCs using immunoaffinity or size difference from other blood cells. This study demonstrates a sheathless, high-throughput separation of CTCs from white blood cells (WBCs) using a viscoelastic fluid. To determine the fluid viscoelasticity and the flow rate for CTC separation, and to validate the device performance, flow characteristics of 6, 13, and 27 μm particles in viscoelastic fluids with various concentrations were estimated at different flow rates. Using 0.2% hyaluronic acid (HA) solution, MCF-7 (Michigan Cancer Foundation-7) cells mimicking CTCs in this study were successfully separated from WBCs at 500 μL/min with a separation efficiency of 94.8%. Small amounts of MCF-7 cells (~5.2%) were found at the center outlet due to the size overlap with WBCs.

Effects of electrothermal vortices on insulator-based dielectrophoresis for circulating tumor cell separation

2017 ◽  
Vol 39 (5-6) ◽  
pp. 869-877 ◽  
Author(s):  
Arian Aghilinejad ◽  
Mohammad Aghaamoo ◽  
Xiaolin Chen ◽  
Jie Xu
Keyword(s):  
Tumor Cell ◽  
Cell Separation ◽  
Circulating Tumor Cell
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