scholarly journals Biodegradation of used lubricant oil hydrocarbons using Bacillus subtilis InaCC B289 and Pseudomonas aeruginosa InaCC B290 in single or mixed cultures

2018 ◽  
Author(s):  
Dewi Rista Kurnia ◽  
Wibowo Mangunwardoyo ◽  
Hanies Ambarsari
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Mixed Cultures ◽  
Lubricant Oil ◽  
Oil Hydrocarbons ◽  
Used Lubricant Oil

Mikrobizide und viruzide Aufbereitung von Dialysegeräten

2018 ◽  
Vol 22 (02) ◽  
pp. 82-89
Author(s):  
Friedrich von Rheinbaben ◽  
Oliver Riebe ◽  
Johanna Köhnlein ◽  
Sebastian Werner
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Phase 3 ◽  
Aspergillus Brasiliensis

ZusammenfassungZentrales Bauteil des Genius® 90 Therapie Systems ist der sogenannte Genius-Tank, dem die frische Dialyseflüssigkeit entnommen und in den die verbrauchte Lösung nach der Dialyse zurückgeführt wird. Daher kommt der sicheren Aufbereitung des Systems eine besondere Bedeutung zu. Hierfür wird ein Aufbereitungsverfahren unter Verwendung von UV-Licht in Kombination mit einem chemischen Desinfektionsmittel angewendet. Ziel der hier beschriebenen Untersuchung war es, die Wirkungsbreite und Wirkungstiefe dieses Aufbereitungsverfahrens unter praxisnahen Phase-3-Bedingungen zu ermitteln. Dazu wurde das Gerät mit Mikroorganismen und Viren künstlich kontaminiert und die Wirkung der einzelnen Verfahrensschritte ermittelt. Im Gegensatz zu der üblichen Vorgehensweise praxisnaher Untersuchungen machen Aufbereitungsverfahren medizinischer Geräte unter Phase-3-Kriterien meist eine neuartige Arbeitsweise erforderlich – im Falle der hier vorgestellten Untersuchung sogar die Konstruktion eines speziellen Geräts zur Platzierung von Keimträgen im Genius-Tank. Im Ergebnis konnte gezeigt werden, dass bereits UV-Licht allein sowie in Kombination mit einem chemischen Desinfektionsmittel unter praxisnahen Bedingungen eine sichere Wirksamkeit gegen Bakterien (Pseudomonas aeruginosa) und bakterielle Sporen (Bacillus subtilis), Schimmelpilze (Aspergillus brasiliensis) und Viren (Murines Parvovirus) besitzt.

The Study of Bacillus Subtils Antimicrobial Activity on Some of the Pathological Isolates

2019 ◽  
Vol 9 (02) ◽  
Author(s):  
Hussein A Kadhum ◽  
Thualfakar H Hasan2
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Bacterial Growth ◽  
Broad Spectrum ◽  
Inhibitory Activity ◽  
Bacterial Pathogens ◽  
Inhibitory Ability ◽  
Selection Of

The study involved the selection of two isolates from Bacillus subtilis to investigate their inhibitory activity against some bacterial pathogens. B sub-bacteria were found to have a broad spectrum against test bacteria such as Staphylococcus aureus and Pseudomonas aeruginosa. They were about 23-30 mm and less against Klebsiella sp. The sensitivity of some antibodies was tested on the test samples. The results showed that the inhibitory ability of bacterial growth in the test samples using B. subtilis extract was more effective than the antibiotics used.

scholarly journals Physicochemical, Bacteriological and Parasitological Examination of Selected Fish Pond Water Samples in Awka and Its Environment Anambra State, Nigeria

2020 ◽  
pp. 27-48
Author(s):  
Umeh Odera Richard ◽  
E. I. Chukwura ◽  
Ibo Eziafakaego Mercy
Keyword(s):  
Water Quality ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Pseudomonas Fluorescens ◽  
Vibrio Cholerae ◽  
Vibrio Parahaemolyticus ◽  
Acinetobacter Calcoaceticus ◽  
Salmonella Typhi ◽  
Fish Pond

A fish pond with recommended water quality will produce healthy fishes. Fish ponds with poor water quality will cause fish mortality and outbreak of diseases to fish consumers. Physicochemical analysis was done using standard analytical methods, the total bacterial count was determined by dilution and membrane filtration techniques. Parasitological analysis was done using the centrifugation method. A total of fifteen well waters were sampled during wet season. Results showed that the temperature ranged from 27°C to 29°C, pH, 6.21 to 8.15; dissolved oxygen, 4.28 mg/l to 5.78 mg/l, electrical conductivity, 166.36 µs/cm to 394.00 µs/cm; total dissolved solids, 41 mg/l to 121 mg/l; total suspended solids, 1.00 mg/l to 19.40 mg/l; total solids, 42.00 mg/l to 140.4 mg/l; turbidity values, 7.01 NTU to 10.36 NTU; nitrate, 3.10 mg/l to 28.00 mg/l; total alkalinity, 36 mg/l to 91 mg/l; phosphate, 1.26 mg/l to 13.11 mg/l; sulphate, 0.39 mg/l to 4.37 mg/l; total chloride, 7.08 mg/l to 14.19 mg/l; carbonates, 1.33 mg/l to 2.35 mg/l; bicarbonates, 34.59 mg/l to 89.38 mg/l; total hardness, 25.31 mg/l to 53.04 mg/l; calcium hardness, 23.94 mg/l to 51.96 mg/l; magnesium hardness, 1.08 mg/l to 4.20 mg/l; total acidity, 2 mg/l to 22 mg/l; potassium, 0.04 mg/l to 2.23 mg/l; cadmium, 0.00 mg/l to 0.04 mg/l; lead, 0.01 mg/l - 0.16 mg/l; chromium, 0.00 mg/l - 0.03 mg/l; mercury was not detected, copper, 0.00 mg/l - 0.04 mg/l; arsenic, 0.00 mg/l - 0.02 mg/l; zinc, 0.00 mg/l to 0.02 mg/l; iron, 0.01 mg/l - 1.19 mg/l. The total bacterial counts ranged from 3.60-4.12 log cfu/ml; total coliforms, 14-46 cfu/100ml, Vibrio cholerae, 0-11 cfu/100ml; Vibrio parahaemolyticus, 0-15 cfu/100ml; faecal coliform, 1-9 cfu/100 ml; Acinetobacter calcoaceticus, 0-8 cfu/100 ml; Bacillus subtilis, 0-9 cfu/ml; Staphylococcus aureus, 0-5 cfu/ml; Pseudomonas aeruginosa, 0-12 cfu/100 ml; Pseudomonas fluorescens, 0-12 cfu/100 ml and Clostridium perfringens were not detected in any of the samples. Twelve bacterial species namely Klebsiella pneumoniae, Acinetobacter calcoaceticus, Escherichia coli, Staphylococcus aureus, Vibrio cholerae, Pseudomonas fluorescens, Pseudomonas aeruginosa, Proteus mirabilis, Vibrio parahaemolyticus, Bacillus subtilis, Shigella flexineri and Salmonella typhi were isolated and identified using standard analytical and molecular procedures. Parasites identified were Ichthyobodo species, Diplostomum species, Myxobolus species, Chilodonella species, Bothriocephalus species, Ambiphrya species and Leech species. Salmonella typhi had the highest frequency of isolation (20.63%) while Acinetobacter calcoaceticus and Staphylococcus aureus had the lowest frequency of isolation (2.83%). Ichthyobodo species had the highest frequency of isolation (21.43%) while Leech species had the lowest frequency of isolation (5.71%). Some of the physicochemical, bacteriological and parasitological parameters had values above World Health Organization admissible limits and therefore proper sanitary practices and water treatments must be employed to prevent epidemic among fish consumers.

XÁC ĐỊNH THÀNH PHẦN HÓA HỌC VÀ KHẢ NĂNG KHÁNG VI SINH VẬT CỦA CAO CHIẾT ETHANOL LY TRÍCH TỪ THÂN RỄ LOÀI THIÊN NIÊN KIỆN PI-E (HOMALOMENA PIERREANA)

2020 ◽  
Vol 39 (03) ◽  
Author(s):  
VĂN HỒNG THIỆN ◽  
LÊ BÍCH TRÂM ◽  
NGUYỄN THANH LAN ◽  
HỒ NGUYỄN HOÀNG YẾN ◽  
LƯU HỒNG TRƯỜNG ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Salmonella Typhimurium ◽  
Salmonella Enteritidis

Nghiên cứu này đã xác định được mẫu nghiên cứu thu tại Vườn quốc gia Phú Quốc là loài Homalomena pierreana. Thông qua kỹ thuật sắc ký ghép khối phổ, 10 hợp chất thuộc nhóm sesquiterpene có trong cao chiết ethanol của thân rễ loài H. pierreana đã được xác định. Ngoài ra, cao chiết ethanol từ mẫu nghiên cứu cũng cho thấy khả năng kháng lại 6 chủng vi khuẩn kiểm định là Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella enteritidis và Salmonella typhimurium.

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