scholarly journals Biodegradation of diesel oil hydrocarbons using Bacillus subtilis InaCC B289 and Pseudomonas aeruginosa InaCC B290 in single and mixed cultures

2018 ◽  
Author(s):  
Rina Marita Safitri ◽  
Wibowo Mangunwardoyo ◽  
Hanies Ambarsari
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Diesel Oil ◽  
Mixed Cultures ◽  
Oil Hydrocarbons ◽  
Single And Mixed Cultures

Mikrobizide und viruzide Aufbereitung von Dialysegeräten

2018 ◽  
Vol 22 (02) ◽  
pp. 82-89
Author(s):  
Friedrich von Rheinbaben ◽  
Oliver Riebe ◽  
Johanna Köhnlein ◽  
Sebastian Werner
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Phase 3 ◽  
Aspergillus Brasiliensis

ZusammenfassungZentrales Bauteil des Genius® 90 Therapie Systems ist der sogenannte Genius-Tank, dem die frische Dialyseflüssigkeit entnommen und in den die verbrauchte Lösung nach der Dialyse zurückgeführt wird. Daher kommt der sicheren Aufbereitung des Systems eine besondere Bedeutung zu. Hierfür wird ein Aufbereitungsverfahren unter Verwendung von UV-Licht in Kombination mit einem chemischen Desinfektionsmittel angewendet. Ziel der hier beschriebenen Untersuchung war es, die Wirkungsbreite und Wirkungstiefe dieses Aufbereitungsverfahrens unter praxisnahen Phase-3-Bedingungen zu ermitteln. Dazu wurde das Gerät mit Mikroorganismen und Viren künstlich kontaminiert und die Wirkung der einzelnen Verfahrensschritte ermittelt. Im Gegensatz zu der üblichen Vorgehensweise praxisnaher Untersuchungen machen Aufbereitungsverfahren medizinischer Geräte unter Phase-3-Kriterien meist eine neuartige Arbeitsweise erforderlich – im Falle der hier vorgestellten Untersuchung sogar die Konstruktion eines speziellen Geräts zur Platzierung von Keimträgen im Genius-Tank. Im Ergebnis konnte gezeigt werden, dass bereits UV-Licht allein sowie in Kombination mit einem chemischen Desinfektionsmittel unter praxisnahen Bedingungen eine sichere Wirksamkeit gegen Bakterien (Pseudomonas aeruginosa) und bakterielle Sporen (Bacillus subtilis), Schimmelpilze (Aspergillus brasiliensis) und Viren (Murines Parvovirus) besitzt.

The Study of Bacillus Subtils Antimicrobial Activity on Some of the Pathological Isolates

2019 ◽  
Vol 9 (02) ◽  
Author(s):  
Hussein A Kadhum ◽  
Thualfakar H Hasan2
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Bacterial Growth ◽  
Broad Spectrum ◽  
Inhibitory Activity ◽  
Bacterial Pathogens ◽  
Inhibitory Ability ◽  
Selection Of

The study involved the selection of two isolates from Bacillus subtilis to investigate their inhibitory activity against some bacterial pathogens. B sub-bacteria were found to have a broad spectrum against test bacteria such as Staphylococcus aureus and Pseudomonas aeruginosa. They were about 23-30 mm and less against Klebsiella sp. The sensitivity of some antibodies was tested on the test samples. The results showed that the inhibitory ability of bacterial growth in the test samples using B. subtilis extract was more effective than the antibiotics used.

scholarly journals Physicochemical, Bacteriological and Parasitological Examination of Selected Fish Pond Water Samples in Awka and Its Environment Anambra State, Nigeria

2020 ◽  
pp. 27-48
Author(s):  
Umeh Odera Richard ◽  
E. I. Chukwura ◽  
Ibo Eziafakaego Mercy
Keyword(s):  
Water Quality ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Pseudomonas Fluorescens ◽  
Vibrio Cholerae ◽  
Vibrio Parahaemolyticus ◽  
Acinetobacter Calcoaceticus ◽  
Salmonella Typhi ◽  
Fish Pond

A fish pond with recommended water quality will produce healthy fishes. Fish ponds with poor water quality will cause fish mortality and outbreak of diseases to fish consumers. Physicochemical analysis was done using standard analytical methods, the total bacterial count was determined by dilution and membrane filtration techniques. Parasitological analysis was done using the centrifugation method. A total of fifteen well waters were sampled during wet season. Results showed that the temperature ranged from 27°C to 29°C, pH, 6.21 to 8.15; dissolved oxygen, 4.28 mg/l to 5.78 mg/l, electrical conductivity, 166.36 µs/cm to 394.00 µs/cm; total dissolved solids, 41 mg/l to 121 mg/l; total suspended solids, 1.00 mg/l to 19.40 mg/l; total solids, 42.00 mg/l to 140.4 mg/l; turbidity values, 7.01 NTU to 10.36 NTU; nitrate, 3.10 mg/l to 28.00 mg/l; total alkalinity, 36 mg/l to 91 mg/l; phosphate, 1.26 mg/l to 13.11 mg/l; sulphate, 0.39 mg/l to 4.37 mg/l; total chloride, 7.08 mg/l to 14.19 mg/l; carbonates, 1.33 mg/l to 2.35 mg/l; bicarbonates, 34.59 mg/l to 89.38 mg/l; total hardness, 25.31 mg/l to 53.04 mg/l; calcium hardness, 23.94 mg/l to 51.96 mg/l; magnesium hardness, 1.08 mg/l to 4.20 mg/l; total acidity, 2 mg/l to 22 mg/l; potassium, 0.04 mg/l to 2.23 mg/l; cadmium, 0.00 mg/l to 0.04 mg/l; lead, 0.01 mg/l - 0.16 mg/l; chromium, 0.00 mg/l - 0.03 mg/l; mercury was not detected, copper, 0.00 mg/l - 0.04 mg/l; arsenic, 0.00 mg/l - 0.02 mg/l; zinc, 0.00 mg/l to 0.02 mg/l; iron, 0.01 mg/l - 1.19 mg/l. The total bacterial counts ranged from 3.60-4.12 log cfu/ml; total coliforms, 14-46 cfu/100ml, Vibrio cholerae, 0-11 cfu/100ml; Vibrio parahaemolyticus, 0-15 cfu/100ml; faecal coliform, 1-9 cfu/100 ml; Acinetobacter calcoaceticus, 0-8 cfu/100 ml; Bacillus subtilis, 0-9 cfu/ml; Staphylococcus aureus, 0-5 cfu/ml; Pseudomonas aeruginosa, 0-12 cfu/100 ml; Pseudomonas fluorescens, 0-12 cfu/100 ml and Clostridium perfringens were not detected in any of the samples. Twelve bacterial species namely Klebsiella pneumoniae, Acinetobacter calcoaceticus, Escherichia coli, Staphylococcus aureus, Vibrio cholerae, Pseudomonas fluorescens, Pseudomonas aeruginosa, Proteus mirabilis, Vibrio parahaemolyticus, Bacillus subtilis, Shigella flexineri and Salmonella typhi were isolated and identified using standard analytical and molecular procedures. Parasites identified were Ichthyobodo species, Diplostomum species, Myxobolus species, Chilodonella species, Bothriocephalus species, Ambiphrya species and Leech species. Salmonella typhi had the highest frequency of isolation (20.63%) while Acinetobacter calcoaceticus and Staphylococcus aureus had the lowest frequency of isolation (2.83%). Ichthyobodo species had the highest frequency of isolation (21.43%) while Leech species had the lowest frequency of isolation (5.71%). Some of the physicochemical, bacteriological and parasitological parameters had values above World Health Organization admissible limits and therefore proper sanitary practices and water treatments must be employed to prevent epidemic among fish consumers.

scholarly journals Laboratory Study for Biodegradation of Oxymatrine Insecticide by Single and Mixed Cultures of Fungi Isolated from Agriculture Soils in Basrah Governorate, Iraq

2019 ◽  
Vol 16 (1) ◽  
pp. 0010
Author(s):  
Raheem Et al.
Keyword(s):  
Laboratory Study ◽  
Degradation Rate ◽  
Soil Fungi ◽  
Mixed Cultures ◽  
Baseline Study ◽  
Biodegradation Process ◽  
Single And Mixed Cultures

This study focuses on the biodegradation of oxymatrine insecticide by some soil fungi isolated from four agriculture stations. The results showed that the highest degradation rate 94.66% was recorded by Ulocladium sp. at 10 days and A. niger recorded the lowest degradation rate 45.86%, while at 20 days Ulocladium sp. also showed the highest degradation rate 94.98% and the lowest degradation rate reached to 82.49% with A.niger. The mix (Exerohilum sp.+Ulocladium sp.) recorded the highest degradation rate of oxymatrine insecticide 90.22%, 88.51%, 85.34%  at 4, 8 and 12 ppm.The use of mixed isolates enhanced the biodegradation process. There is no study of oxymatrine biodegradation so this study is the first of its kind in the region which can be used as a baseline study for incoming studies

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