Backscattered electron detector for 3D microstructure visualization in scanning electron microscopy

2019 ◽  
Vol 90 (2) ◽  
pp. 023701 ◽  
Author(s):  
E. I. Rau ◽  
V. Yu. Karaulov ◽  
S. V. Zaitsev
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Detector ◽  
3D Microstructure ◽  
Backscattered Electron ◽  
Scanning Electron

Reconstruction algorithm of 3D surface in scanning electron microscopy with backscattered electron detector

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Andrey A. Borzunov ◽  
Dmitry V. Lukyanenko ◽  
Eduard I. Rau ◽  
Anatoly G. Yagola
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Smooth Surface ◽  
Three Dimensional ◽  
Reconstruction Algorithm ◽  
Electron Detector ◽  
Backscattered Electron ◽  
A New Technique ◽  
Dimensional Surface ◽  
Scanning Electron

Abstract A new technique for three-dimensional surface reconstruction of relatively smooth surface topography using the scanning electron microscopy with backscattered electron detector is considered. Experiments show high effectiveness of the method.

scholarly journals Helical fibrillar microstructure of tendon using serial block-face scanning electron microscopy and a mechanical model for interfibrillar load transfer

2019 ◽  
Vol 16 (160) ◽  
pp. 20190547 ◽  
Author(s):  
Babak N. Safa ◽  
John M. Peloquin ◽  
Jessica R. Natriello ◽  
Jeffrey L. Caplan ◽  
Dawn M. Elliott
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Load Transfer ◽  
Three Dimensional ◽  
Helical Structures ◽  
3D Microstructure ◽  
Face Scanning ◽  
Block Face ◽  
Matrix Shear ◽  
Scanning Electron

Tendon's hierarchical structure allows for load transfer between its fibrillar elements at multiple length scales. Tendon microstructure is particularly important, because it includes the cells and their surrounding collagen fibrils, where mechanical interactions can have potentially important physiological and pathological contributions. However, the three-dimensional (3D) microstructure and the mechanisms of load transfer in that length scale are not known. It has been postulated that interfibrillar matrix shear or direct load transfer via the fusion/branching of small fibrils are responsible for load transfer, but the significance of these mechanisms is still unclear. Alternatively, the helical fibrils that occur at the microstructural scale in tendon may also mediate load transfer; however, these structures are not well studied due to the lack of a three-dimensional visualization of tendon microstructure. In this study, we used serial block-face scanning electron microscopy to investigate the 3D microstructure of fibrils in rat tail tendon. We found that tendon fibrils have a complex architecture with many helically wrapped fibrils. We studied the mechanical implications of these helical structures using finite-element modelling and found that frictional contact between helical fibrils can induce load transfer even in the absence of matrix bonding or fibril fusion/branching. This study is significant in that it provides a three-dimensional view of the tendon microstructure and suggests friction between helically wrapped fibrils as a mechanism for load transfer, which is an important aspect of tendon biomechanics.

Sign in / Sign up

Export Citation Format

Share Document