Label-free thermoacoustic imaging of human blood vessels in vivo

2018 ◽  
Vol 113 (25) ◽  
pp. 253702 ◽  
Author(s):  
Zhu Zheng ◽  
Lin Huang ◽  
Huabei Jiang
Keyword(s):  
Blood Vessels ◽  
Human Blood ◽  
Label Free ◽  
Thermoacoustic Imaging

scholarly journals An in vivo model allowing continuous observation of human vascular formation in the same animal over time

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yohei Tsukada ◽  
Fumitaka Muramatsu ◽  
Yumiko Hayashi ◽  
Chiaki Inagaki ◽  
Hang Su ◽  
...  
Keyword(s):  
Blood Vessel ◽  
Blood Vessels ◽  
Human Blood ◽  
Molecular Mechanisms ◽  
Human Tumor ◽  
Continuous Observation ◽  
In Vivo Models ◽  
Cranial Window ◽  
Pathological Conditions

AbstractAngiogenesis contributes to numerous pathological conditions. Understanding the molecular mechanisms of angiogenesis will offer new therapeutic opportunities. Several experimental in vivo models that better represent the pathological conditions have been generated for this purpose in mice, but it is difficult to translate results from mouse to human blood vessels. To understand human vascular biology and translate findings into human research, we need human blood vessel models to replicate human vascular physiology. Here, we show that human tumor tissue transplantation into a cranial window enables engraftment of human blood vessels in mice. An in vivo imaging technique using two-photon microscopy allows continuous observation of human blood vessels until at least 49 days after tumor transplantation. These human blood vessels make connections with mouse blood vessels as shown by the finding that lectin injected into the mouse tail vein reaches the human blood vessels. Finally, this model revealed that formation and/or maintenance of human blood vessels depends on VEGFR2 signaling. This approach represents a useful tool to study molecular mechanisms of human blood vessel formation and to test effects of drugs that target human blood vessels in vivo to show proof of concept in a preclinical model.

Integrin αIIbβ3 Regulates Platelet-Procoagulant Activity in the Lung

Blood ◽  
2020 ◽  
Vol 136 (Supplement 1) ◽  
pp. 32-32
Author(s):  
Tomasz Brzoska ◽  
Tomasz W. Kaminski ◽  
Ravi Vats ◽  
Egemen Tutuncuoglu ◽  
Mark T Gladwin ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Human Blood ◽  
Conflicts Of Interest ◽  
Thrombus Formation ◽  
Procoagulant Activity ◽  
Microfluidic Channels ◽  
High Morbidity ◽  
Pulmonary Thrombosis ◽  
Pulmonary Arteriole

Pulmonary thrombosis is a major complication associated with high morbidity. Despite advances in diagnosis and treatment, the pathophysiology of pulmonary thrombosis remains incompletely understood. New clinical evidence suggests that in situ platelet activation resulting in enhanced procoagulant activity may promote pulmonary thrombosis. Improved understanding of the etiological mechanism would enable the development of new therapies for pulmonary thrombosis. Collagen and thromboplastin (TF) were administered intravascularly (IV) to C57BL/6 (WT) mice and the pulmonary microcirculation was visualized using quantitative fluorescence intravital fluorescence lung microscopy (qFILM). Fluorochrome-conjugated anti-mouse CD49b Ab and dextran was administered IV for in vivo staining of circulating platelets and visualization of blood vessels, respectively. Pulmonary thrombosis was defined as occlusion of blood vessels with platelet aggregates leading to pulmonary ischemia. Additionally, quantitative microfluidic fluorescence microscopy (qMFM) was used to study the effect of platelet αIIbβ3 inhibition on platelet procoagulant activity in human blood under vascular mimetic flow conditions. Collagen and TF triggered dose-dependent pulmonary thrombosis in mice in vivo, which involved development of platelet-rich thrombi in the pulmonary arteriolar bottlenecks (junction of pulmonary arteriole and capillaries), resulting in a transient ischemia in the arteriole and the down-stream capillary tree. The pulmonary arteriole thrombosis triggered by IV collagen or TF was protracted, lethal and completely abrogated following IV administration of αIIbβ3 receptor inhibitor (eptifibatide). Inhibition of platelet αIIbβ3 also significantly reduced platelet procoagulant activity, fibrin formation and thrombus formation in human blood flowing through microfluidic channels ex vivo. Our current findings suggest that αIIbβ3-dependent platelet procoagulant activity promotes pulmonary thrombosis. Both our models have potential application in investigating the molecular determinants of pulmonary thrombosis in diverse pulmonary disorders as well as evaluating efficacy of new antithrombotic drugs. Disclosures No relevant conflicts of interest to declare.

Growth of Human Blood Vessels in Severe Combined Immunodefi cient Mice: A New In Vivo Model System of Angiogenesis

Wound Healing ◽  
2003 ◽  
pp. 161-178
Author(s):  
Peter J. Polverini ◽  
Jacques E. Nör ◽  
Martin C. Peters ◽  
David J. Mooney
Keyword(s):  
Blood Vessels ◽  
Human Blood ◽  
Model System ◽  
In Vivo Model

The Pharmacology of Human Blood Vessels in vivo

1995 ◽  
Vol 32 (1) ◽  
pp. 2-15 ◽  
Author(s):  
David J. Webb
Keyword(s):  
Blood Vessels ◽  
Human Blood

scholarly journals In vivo label-free observation of tumor-related blood vessels in small animals using a newly designed photoacoustic 3D imaging system

2021 ◽  
Author(s):  
Yasufumi Asao ◽  
Ken-ichi Nagae ◽  
Koichi Miyasaka ◽  
Hiroyuki Sekiguchi ◽  
Sadakazu Aiso ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Imaging System ◽  
Photoacoustic Imaging ◽  
Experimental System ◽  
Test Target ◽  
Label Free ◽  
Non Invasive ◽  
Optical Test ◽  
Ultrasound Sensor

Introduction: Photoacoustic technology can be used for non-invasive imaging of blood vessels. In this paper, we report on our prototype photoacoustic imaging system with a newly designed ultrasound sensor and its visualization performance of microvascular in animal. Methods: We fabricated an experimental system for animals using a high-frequency sensor. The system has two modes: still image mode by wide scanning and moving image mode by small rotation of sensor array. Optical test target, euthanized mice and rats, and live mice were used as objects. Results: The results of optical test target showed that the spatial resolution was about 2 times higher than that of our conventional prototype. The image performance in vivo was evaluated in euthanized healthy mice and rats, allowing visualization of detailed blood vessels in the liver and kidneys. In tumor-bearing mice, different results of vascular induction were shown depending on the type of tumor and the method of transplantation. By utilizing the video imaging function, we were able to observe the movement of blood vessels around the tumor. Conclusion: We have demonstrated the feasibility of the system as a less invasive animal experimental device, as it can acquire vascular images in animals in a non-contrast and non-invasive manner.

The Distribution of Vasculokinase in Human Blood Vessels

1962 ◽  
Vol 08 (01) ◽  
pp. 096-100
Author(s):  
Marvin Murray ◽  
Robert Johnson
Keyword(s):  
Inferior Vena Cava ◽  
Superior Mesenteric Artery ◽  
Blood Vessels ◽  
Human Blood ◽  
Iliac Artery ◽  
Popliteal Artery ◽  
Mesenteric Artery ◽  
Inferior Vena ◽  
Vena Cava ◽  
High Concentrations

Summary133 blood vessels were evaluated for vasculokinase concentration in the freshly morbid state. High concentrations of activity were found in the aorta, iliac artery, superior mesenteric artery and popliteal artery. Activity was occasionally found in the inferior vena cava and common iliacs veins. Other vessels evaluated had no activity. Evaluation of the data with respect to vas-culokinase activity and atherosclerosis suggests higher levels of vasculokinase in those vessels having atherosclerosis.

In Vivo Spin Trapping of Glyceryl Trinitrate–Derived Nitric Oxide in Rabbit Blood Vessels and Organs

Circulation ◽  
1995 ◽  
Vol 92 (7) ◽  
pp. 1876-1882 ◽  
Author(s):  
Alexander Mülsch ◽  
Peter Mordvintcev ◽  
Eberhard Bassenge ◽  
Frank Jung ◽  
Bernd Clement ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Glyceryl Trinitrate ◽  
Blood Vessels ◽  
Spin Trapping ◽  
Rabbit Blood
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