A versatile optical microscope for time-dependent single-molecule and single-particle spectroscopy

2018 ◽  
Vol 148 (12) ◽  
pp. 123316 ◽  
Author(s):  
Hao Li ◽  
Haw Yang
Keyword(s):  
Single Molecule ◽  
Single Particle ◽  
Optical Microscope ◽  
Time Dependent ◽  
Single Particle Spectroscopy

Deposition of anionic conjugated poly(phenylenevinylene) onto silica nanoparticles via electrostatic interactions — Assembly and single-particle spectroscopy

2011 ◽  
Vol 89 (3) ◽  
pp. 385-394 ◽  
Author(s):  
An T. Ngo ◽  
Kai L. Lau ◽  
Jeffrey S. Quesnel ◽  
Robert Aboukhalil ◽  
Gonzalo Cosa
Keyword(s):  
Single Molecule ◽  
Single Particle ◽  
Electrostatic Interactions ◽  
Emission Spectra ◽  
Fluorescent Nanoparticles ◽  
Oxygen Scavenger ◽  
Conjugated Polyelectrolyte ◽  
Exciton Migration ◽  
Silica Bead ◽  
Single Particle Spectroscopy

Fluorescent nanoparticles were prepared via adsorption of the conjugated polyelectrolyte poly[5-methoxy-2-(3-sulfopropoxy)-1,4-phenylenevinylene] (MPS-PPV) onto 50 and 100 nm aminosilane functionalized silica beads. The particles were investigated via ensemble and single-molecule or -particle spectroscopy techniques to quantify the effect of the silica bead core on the exciton migration efficiency within the polymer. Ensemble emission spectra and ensemble fluorescence quenching studies with methyl viologen are consistent with good exciton migration along the polymer in the polymer-coated bead. The silica nanobead scaffolding preserves the sensitivity of the free polymer and provides a controllable architecture that minimizes nonspecific interactions. Single-particle spectroscopy studies were conducted on particles immobilized onto the positively charged surface of glass cover slips. Particle immobilization enabled us to monitor the effect of oxygen scavenger solutions on individual particles by changing the surrounding solution. The intensity–time trajectories of individual beads provide a mechanism of signal transduction with potential applications in multiplexing studies. Hundreds of individual beads can be imaged in a rapid parallel fashion.

High sensitivity optical microscope for single molecule spectroscopy studies

2004 ◽  
Vol 75 (8) ◽  
pp. 2746-2751 ◽  
Author(s):  
Gabriele Malengo ◽  
Roberto Milani ◽  
Fabio Cannone ◽  
Silke Krol ◽  
Alberto Diaspro ◽  
...  
Keyword(s):  
Single Molecule ◽  
High Sensitivity ◽  
Optical Microscope ◽  
Single Molecule Spectroscopy ◽  
Spectroscopy Studies

Diabatic aspects of single-particle motion within the time-dependent Hartree-Fock theory

1983 ◽  
Vol 314 (3) ◽  
pp. 309-316 ◽  
Author(s):  
W. Cassing ◽  
A. K. Dhar ◽  
A. Lukasiak ◽  
W. N�renberg
Keyword(s):  
Single Particle ◽  
Particle Motion ◽  
Time Dependent ◽  
Hartree Fock ◽  
Single Particle Motion

scholarly journals Probing the variability in oxidation states of magnetite nanoparticles by single-particle spectroscopy

2018 ◽  
Vol 6 (4) ◽  
pp. 875-882 ◽  
Author(s):  
A. Fraile Rodríguez ◽  
C. Moya ◽  
M. Escoda-Torroella ◽  
A. Romero ◽  
A. Labarta ◽  
...  
Keyword(s):  
Oxidation State ◽  
Absorption Spectroscopy ◽  
Magnetite Nanoparticles ◽  
Single Particle ◽  
Cation Distribution ◽  
Oxidation States ◽  
Magnetic Response ◽  
X Ray ◽  
Single Particle Spectroscopy ◽  
X Ray Absorption

Single-particle X-ray absorption spectroscopy reveals that the oxidation state and cation distribution of individual magnetite nanoparticles may be largely heterogeneous even when the macroscopic structural and magnetic response of the ensembles is uniform.

scholarly journals Single-molecule diffusometry reveals no catalysis-induced diffusion enhancement of alkaline phosphatase as proposed by FCS experiments

2020 ◽  
Vol 117 (35) ◽  
pp. 21328-21335
Author(s):  
Zhijie Chen ◽  
Alan Shaw ◽  
Hugh Wilson ◽  
Maxime Woringer ◽  
Xavier Darzacq ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Single Molecule ◽  
Particle Tracking ◽  
Single Particle ◽  
Theoretical Models ◽  
Single Particle Tracking ◽  
Correlation Spectroscopy ◽  
Single Molecule Level ◽  
Diffusion Phenomena ◽  
Diffusion Enhancement

Theoretical and experimental observations that catalysis enhances the diffusion of enzymes have generated exciting implications about nanoscale energy flow, molecular chemotaxis, and self-powered nanomachines. However, contradictory claims on the origin, magnitude, and consequence of this phenomenon continue to arise. To date, experimental observations of catalysis-enhanced enzyme diffusion have relied almost exclusively on fluorescence correlation spectroscopy (FCS), a technique that provides only indirect, ensemble-averaged measurements of diffusion behavior. Here, using an anti-Brownian electrokinetic (ABEL) trap and in-solution single-particle tracking, we show that catalysis does not increase the diffusion of alkaline phosphatase (ALP) at the single-molecule level, in sharp contrast to the ∼20% enhancement seen in parallel FCS experiments usingp-nitrophenyl phosphate (pNPP) as substrate. Combining comprehensive FCS controls, ABEL trap, surface-based single-molecule fluorescence, and Monte Carlo simulations, we establish thatpNPP-induced dye blinking at the ∼10-ms timescale is responsible for the apparent diffusion enhancement seen in FCS. Our observations urge a crucial revisit of various experimental findings and theoretical models––including those of our own––in the field, and indicate that in-solution single-particle tracking and ABEL trap are more reliable means to investigate diffusion phenomena at the nanoscale.

Single particle ICP-MS-based absolute and relative quantification ofE. coliO157 16S rRNA using sandwich hybridization capture

The Analyst ◽  
2019 ◽  
Vol 144 (5) ◽  
pp. 1725-1730 ◽  
Author(s):  
Xiaomin Xu ◽  
Jiyun Chen ◽  
Bangrui Li ◽  
Lijuan Tang ◽  
Jianhui Jiang
Keyword(s):  
16S Rrna ◽  
Single Molecule ◽  
Single Particle ◽  
Inductively Coupled Plasma ◽  
Inductively Coupled ◽  
Hybridization Capture ◽  
Sandwich Hybridization ◽  
Icp Ms ◽  
Magnetic Capture ◽  
Plasma Mass Spectrometry

Herein, a novel 16S rRNA detection platform was achieved by combining a sandwich hybridization reaction, a single-molecule magnetic capture, and single particle-inductively coupled plasma mass spectrometry amplification.

scholarly journals Automated single particle detection and tracking for large microscopy datasets

2016 ◽  
Vol 3 (5) ◽  
pp. 160225 ◽  
Author(s):  
Rhodri S. Wilson ◽  
Lei Yang ◽  
Alison Dun ◽  
Annya M. Smyth ◽  
Rory R. Duncan ◽  
...  
Keyword(s):  
Single Molecule ◽  
Single Particle ◽  
Image Data ◽  
Ground Truth ◽  
Detection Algorithm ◽  
Large Datasets ◽  
Single Particle Tracking ◽  
Synthetic Image ◽  
Particle Detection ◽  
Very Large Datasets

Recent advances in optical microscopy have enabled the acquisition of very large datasets from living cells with unprecedented spatial and temporal resolutions. Our ability to process these datasets now plays an essential role in order to understand many biological processes. In this paper, we present an automated particle detection algorithm capable of operating in low signal-to-noise fluorescence microscopy environments and handling large datasets. When combined with our particle linking framework, it can provide hitherto intractable quantitative measurements describing the dynamics of large cohorts of cellular components from organelles to single molecules. We begin with validating the performance of our method on synthetic image data, and then extend the validation to include experiment images with ground truth. Finally, we apply the algorithm to two single-particle-tracking photo-activated localization microscopy biological datasets, acquired from living primary cells with very high temporal rates. Our analysis of the dynamics of very large cohorts of 10 000 s of membrane-associated protein molecules show that they behave as if caged in nanodomains. We show that the robustness and efficiency of our method provides a tool for the examination of single-molecule behaviour with unprecedented spatial detail and high acquisition rates.

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