Dual-blade-like shaped nanostructured metasurface inducing dual-band extinction for dye fluorescence enhancement

2018 ◽  
Vol 123 (4) ◽  
pp. 043104 ◽  
Author(s):  
Khai Q. Le
2019 ◽  
Vol 126 (5) ◽  
pp. 636
Author(s):  
А.А. Намыкин ◽  
А.П. Хороводов ◽  
О.В. Семячкина-Глушковская ◽  
В.В. Тучин ◽  
И.В. Федосов

AbstractThe effect of photoinduced enhancement of Evans blue (EB) dye fluorescence in blood plasma and albumin solution has been considered. Manifestations of the dye fluorescence enhancement in the albumin solution and rat blood plasma have been compared. Saturation of photoinduced fluorescence, the effect of enhanced fluorescence delay, and divergence in fluorescence spectra of the solutions have been found for the first time. Based on the obtained results, a hypothesis about the presence of nonfluorescent complexes with the EB participation in blood plasma is proposed.


1997 ◽  
Vol 51 (7) ◽  
pp. 1002-1007 ◽  
Author(s):  
Xiang-Qun Guo ◽  
Zu-Lin Zhang ◽  
Yi-Bing Zhao ◽  
Dong-Yuan Wang ◽  
Jin-Gou Xu

In this paper, the investigation of DNA–dye fluorescence enhancement based on shifting the dimer–monomer equilibrium of a fluorescent dye, acridine orange (AO), is reported. Formation of a virtually nonfluorescent dimeric dye, acridine orange homodimer (AOAO), induced by the pre-micellar aggregation of an anionic surfactant, sodium dodecyl sulfate (SDS), was observed. The possibility of using the in situ formed AOAO as a fluorescent probe for nucleic acids and polynucleotides was studied. The results showed that a nearly 1000-fold fluorescence enhancement was observed upon addition of calf thymus DNA (CT DNA). The fluorescence enhancement effect of DNA was thought to be based on the DNA modulated shift of the dimmer monomer equilibrium of AO in the anionic surfactant solution. Intercalation of the monomer in DNA caused the dissociation of AOAO and led to a very high fluorescence enhancement. It seemed that the dimeric dye molecules acted as a source of monomer molecules ready for interacting with nucleic acids and, at the same time, decreased the inherent fluorescence of monomer molecules, which proved to be unfavorable to the detection of fluorescence enhancement. A linear dependence of fluorescence intensity on CT DNA concentration over a range from 7.8 ng/mL to 10.0 g/mL, in the presence of AO at a concentration of 1.65 × 10−6mol/L and of SDS at a concentration of 8.0 × 10−4 mol/L, allowed sensitive quantitation of CT DNA in a conventional fluorometer. Calibration graphs for yeast RNA and polynucleotides, such as poly A, poly U, and poly I, were also obtained.


Author(s):  
Barbara Trask ◽  
Susan Allen ◽  
Anne Bergmann ◽  
Mari Christensen ◽  
Anne Fertitta ◽  
...  

Using fluorescence in situ hybridization (FISH), the positions of DNA sequences can be discretely marked with a fluorescent spot. The efficiency of marking DNA sequences of the size cloned in cosmids is 90-95%, and the fluorescent spots produced after FISH are ≈0.3 μm in diameter. Sites of two sequences can be distinguished using two-color FISH. Different reporter molecules, such as biotin or digoxigenin, are incorporated into DNA sequence probes by nick translation. These reporter molecules are labeled after hybridization with different fluorochromes, e.g., FITC and Texas Red. The development of dual band pass filters (Chromatechnology) allows these fluorochromes to be photographed simultaneously without registration shift.


2002 ◽  
Vol 149 (1) ◽  
pp. 41-44 ◽  
Author(s):  
L. Economou ◽  
R.J. Langley
Keyword(s):  

2020 ◽  
Vol 91 (3) ◽  
pp. 30901
Author(s):  
Yibo Tang ◽  
Longhui He ◽  
Jianming Xu ◽  
Hailang He ◽  
Yuhan Li ◽  
...  

A dual-band microwave metamaterial absorber with single-peak regulation and wide-angle absorption has been proposed and illustrated. The designed metamaterial absorber is consisted of hollow-cross resonators, solid-cross resonators, dielectric substrate and metallic background plane. Strong absorption peak coefficients of 99.92% and 99.55% are achieved at 8.42 and 11.31 GHz, respectively, which is basically consistent with the experimental results. Surface current density and changing material properties are employed to illustrate the absorptive mechanism. More importantly, the proposed dual-band metamaterial absorber has the adjustable property of single absorption peak and could operate well at wide incidence angles for both transverse electric (TE) and transverse magnetic (TM) waves. Research results could provide and enrich instructive guidances for realizing a single-peak-regulation and wide-angle dual-band metamaterial absorber.


2008 ◽  
Vol 128 (6) ◽  
pp. 878-884
Author(s):  
Nobuhiko Okuzaki ◽  
Yukihiro Shimakata ◽  
Mitsuyuki Yamauchi ◽  
Kouji Wada ◽  
Takashi Iwasaki
Keyword(s):  

Author(s):  
Imane Halkhams ◽  
Said Mazer ◽  
Mahmoud Mehdi ◽  
Moulhime El Bekkali ◽  
Wafae El Hamdani

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