Engineered metallic nanostructures for dye fluorescence enhancement: Experiment and simulation

2019 ◽  
Vol 560 ◽  
pp. 140-145 ◽  
Author(s):  
Khai Q. Le
Keyword(s):  
Fluorescence Enhancement ◽  
Metallic Nanostructures ◽  
Dye Fluorescence ◽  
Experiment And Simulation

scholarly journals Фотоиндуцированное усиление флуоресценции красителя эванса синего в водном растворе альбумина-=SUP=-*-=/SUP=-

2019 ◽  
Vol 126 (5) ◽  
pp. 636
Author(s):  
А.А. Намыкин ◽  
А.П. Хороводов ◽  
О.В. Семячкина-Глушковская ◽  
В.В. Тучин ◽  
И.В. Федосов
Keyword(s):  
Blood Plasma ◽  
Evans Blue ◽  
Fluorescence Spectra ◽  
Fluorescence Enhancement ◽  
Albumin Solution ◽  
Enhanced Fluorescence ◽  
Rat Blood ◽  
Dye Fluorescence ◽  
First Time ◽  
In Blood Plasma

AbstractThe effect of photoinduced enhancement of Evans blue (EB) dye fluorescence in blood plasma and albumin solution has been considered. Manifestations of the dye fluorescence enhancement in the albumin solution and rat blood plasma have been compared. Saturation of photoinduced fluorescence, the effect of enhanced fluorescence delay, and divergence in fluorescence spectra of the solutions have been found for the first time. Based on the obtained results, a hypothesis about the presence of nonfluorescent complexes with the EB participation in blood plasma is proposed.

DNA—Dye Fluorescence Enhancement Based on Shifting the Dimer—Monomer Equilibrium of Fluorescent Dye

1997 ◽  
Vol 51 (7) ◽  
pp. 1002-1007 ◽  
Author(s):  
Xiang-Qun Guo ◽  
Zu-Lin Zhang ◽  
Yi-Bing Zhao ◽  
Dong-Yuan Wang ◽  
Jin-Gou Xu
Keyword(s):  
Nucleic Acids ◽  
Acridine Orange ◽  
Anionic Surfactant ◽  
Fluorescence Enhancement ◽  
Sodium Dodecyl ◽  
Fluorescent Dye ◽  
Enhancement Effect ◽  
Solution Intercalation ◽  
Dye Fluorescence ◽  
Ct Dna

In this paper, the investigation of DNA–dye fluorescence enhancement based on shifting the dimer–monomer equilibrium of a fluorescent dye, acridine orange (AO), is reported. Formation of a virtually nonfluorescent dimeric dye, acridine orange homodimer (AOAO), induced by the pre-micellar aggregation of an anionic surfactant, sodium dodecyl sulfate (SDS), was observed. The possibility of using the in situ formed AOAO as a fluorescent probe for nucleic acids and polynucleotides was studied. The results showed that a nearly 1000-fold fluorescence enhancement was observed upon addition of calf thymus DNA (CT DNA). The fluorescence enhancement effect of DNA was thought to be based on the DNA modulated shift of the dimmer monomer equilibrium of AO in the anionic surfactant solution. Intercalation of the monomer in DNA caused the dissociation of AOAO and led to a very high fluorescence enhancement. It seemed that the dimeric dye molecules acted as a source of monomer molecules ready for interacting with nucleic acids and, at the same time, decreased the inherent fluorescence of monomer molecules, which proved to be unfavorable to the detection of fluorescence enhancement. A linear dependence of fluorescence intensity on CT DNA concentration over a range from 7.8 ng/mL to 10.0 g/mL, in the presence of AO at a concentration of 1.65 × 10−6mol/L and of SDS at a concentration of 8.0 × 10−4 mol/L, allowed sensitive quantitation of CT DNA in a conventional fluorometer. Calibration graphs for yeast RNA and polynucleotides, such as poly A, poly U, and poly I, were also obtained.

A Photoactivable Formaldehyde Donor with Fluorescence Monitoring Reveals Threshold to Arrest Cell Migration

2019 ◽  
Author(s):  
Lukas P Smaga ◽  
Nicholas W Pino ◽  
Gabriela E Ibarra ◽  
Vishnu Krishnamurthy ◽  
Jefferson Chan
Keyword(s):  
Wound Healing ◽  
Cell Migration ◽  
Fluorescence Enhancement ◽  
Biological Effects ◽  
Cellular Systems ◽  
Live Cells ◽  
First Report ◽  
Cell Lysate ◽  
Intracellular Release ◽  
Biological Analytes

Controlled light-mediated delivery of biological analytes enables the investigation of highly reactivity molecules within cellular systems. As many biological effects are concentration dependent, it is critical to determine the location, time, and quantity of analyte donation. In this work, we have developed the first photoactivatable donor for formaldehyde (FA). Our optimized photoactivatable donor, photoFAD-3, is equipped with a fluorescence readout that enables monitoring of FA release with a concomitant 139-fold fluorescence enhancement. Tuning of photostability and cellular retention enabled quantification of intracellular FA release through cell lysate calibration. Application of photoFAD-3 uncovered the concentration range necessary for arresting wound healing in live cells. This marks the first report where a photoactivatable donor for any analyte has been used to quantify intracellular release.

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