scholarly journals Geometric confinement is required for recovery and maintenance of chondrocyte phenotype in alginate

2017 ◽  
Vol 1 (1) ◽  
pp. 016104 ◽  
Author(s):  
Megan E. Cooke ◽  
Mark J. Pearson ◽  
Richard J. A. Moakes ◽  
Christopher J. Weston ◽  
Edward T. Davis ◽  
...  
Keyword(s):  
Chondrocyte Phenotype ◽  
Geometric Confinement

scholarly journals Morphodynamic signatures of MDA-MB-231 single cells and cell doublets undergoing invasion in confined microenvironments

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Xingjian Zhang ◽  
Trevor Chan ◽  
Michael Mak
Keyword(s):  
Single Cell ◽  
Single Cells ◽  
Leading Edge ◽  
Cell Group ◽  
Collective State ◽  
Long Term Effects ◽  
Cell Metastasis ◽  
Geometric Confinement ◽  
Short And Long Term ◽  
The Impact

AbstractCancer cell metastasis is a major factor in cancer-related mortality. During the process of metastasis, cancer cells exhibit migratory phenotypes and invade through pores in the dense extracellular matrix. However, the characterization of morphological and subcellular features of cells in similar migratory phenotypes and the effects of geometric confinement on cell morphodynamics are not well understood. Here, we investigate the phenotypes of highly aggressive MDA-MB-231 cells in single cell and cell doublet (an initial and simplified collective state) forms in confined microenvironments. We group phenotypically similar single cells and cell doublets and characterize related morphological and subcellular features. We further detect two distinct migratory phenotypes, fluctuating and non-fluctuating, within the fast migrating single cell group. In addition, we demonstrate an increase in the number of protrusions formed at the leading edge of cells after invasion through geometric confinement. Finally, we track the short and long term effects of varied degrees of confinement on protrusion formation. Overall, our findings elucidate the underlying morphological and subcellular features associated with different single cell and cell doublet phenotypes and the impact of invasion through confined geometry on cell behavior.

scholarly journals Doublecortin May Play a Role in Defining Chondrocyte Phenotype

2014 ◽  
Vol 15 (4) ◽  
pp. 6941-6960 ◽  
Author(s):  
Dongxia Ge ◽  
Qing-Song Zhang ◽  
Jovanny Zabaleta ◽  
Qiuyang Zhang ◽  
Sen Liu ◽  
...  
Keyword(s):  
Chondrocyte Phenotype

Nanog maintains human chondrocyte phenotype and function in vitro

2009 ◽  
Vol 28 (4) ◽  
pp. 516-521 ◽  
Author(s):  
Hongjun Zheng ◽  
Francoise Gourronc ◽  
Joseph A. Buckwalter ◽  
James A. Martin
Keyword(s):  
Chondrocyte Phenotype ◽  
Human Chondrocyte ◽  
And Function

scholarly journals The effect of geometric confinement on gas separation characteristics of additive poly[3-(trimethylsilyl)tricyclononene-7]

2018 ◽  
Vol 9 (2) ◽  
pp. 252-258
Author(s):  
E.A. Chernova ◽  
M.A. Bermeshev ◽  
D.I. Petukhov ◽  
O.V. Boytsova ◽  
A.V. Lukashin ◽  
...  
Keyword(s):  
Gas Separation ◽  
Geometric Confinement

Cell Motility in Microfabricated Models of the Tissue Microenvironment

2001 ◽  
Author(s):  
Kevin Kit Parker ◽  
Donald E. Ingber
Keyword(s):  
Cell Motility ◽  
Signaling Pathways ◽  
Intracellular Signaling ◽  
Isometric Tension ◽  
Migration Behavior ◽  
Tissue Microenvironment ◽  
Intracellular Signaling Pathways ◽  
Capillary Endothelial Cells ◽  
Geometric Confinement ◽  
Stable Rotation

Abstract We conducted studies using micropatterned substrates to elucidate how cell shape and geometric confinement regulates the inter- and intracellular signaling pathways required for cell motility. When cells were cultured on individual cell-sized square adhesive islands coated with ECM, they extend to the edge of the island and assume a square shape. When these cells were stimulated with growth factors, they preferentially extended lamellipodia from the corners versus the sides. This process was mediated by myosin-generated isometric tension that induced tight spatial localization of Rac in the corners. When two or three capillary endothelial cells are constrained to a fibronectin (FN) island, coordinated cell migration results in stable rotation of the entire system about its center. Thus, the emergent pattern is due to the coordinated migration behavior of the cells. These observations suggest that ECM-induced mechanotransduction potentiates compartmentalized signaling pathways and the multicellular organization required of tissue morphogenesis.

scholarly journals Manipulating the Topology of Nanoscale Skyrmion Bubbles by Spatially Geometric Confinement

ACS Nano ◽  
2019 ◽  
Vol 13 (1) ◽  
pp. 922-929 ◽  
Author(s):  
Zhipeng Hou ◽  
Qiang Zhang ◽  
Guizhou Xu ◽  
Senfu Zhang ◽  
Chen Gong ◽  
...  
Keyword(s):  
Geometric Confinement

scholarly journals Strenuous Treadmill Running Induces a Chondrocyte Phenotype in Rat Achilles Tendons

2016 ◽  
Vol 22 ◽  
pp. 3705-3712 ◽  
Author(s):  
Shao-Yong Xu ◽  
Shu-Fen Li ◽  
Guo-Xin Ni
Keyword(s):  
Treadmill Running ◽  
Chondrocyte Phenotype

scholarly journals Cycloastragenol as an Exogenous Enhancer of Chondrogenic Differentiation of Human Adipose-Derived Mesenchymal Stem Cells. A Morphological Study

Cells ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 347 ◽  
Author(s):  
Marta Anna Szychlinska ◽  
Giovanna Calabrese ◽  
Silvia Ravalli ◽  
Nunziatina Laura Parrinello ◽  
Stefano Forte ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Proliferation ◽  
Mesenchymal Stem Cells ◽  
Chondrogenic Differentiation ◽  
Hydrolysis Product ◽  
Cartilage Regeneration ◽  
Triterpenoid Saponin ◽  
Type I ◽  
Chondrocyte Phenotype

Stem cell therapy and tissue engineering represent a promising approach for cartilage regeneration. However, they present limits in terms of mechanical properties and premature de-differentiation of engineered cartilage. Cycloastragenol (CAG), a triterpenoid saponin compound and a hydrolysis product of the main ingredient in Astragalus membranaceous, has been explored for cartilage regeneration. The aim of this study was to investigate CAG’s ability to promote cell proliferation, maintain cells in their stable active phenotype, and support the production of cartilaginous extracellular matrix (ECM) in human adipose-derived mesenchymal stem cells (hAMSCs) in up to 28 days of three-dimensional (3D) chondrogenic culture. The hAMSC pellets were cultured in chondrogenic medium (CM) and in CM supplemented with CAG (CAG–CM) for 7, 14, 21, and 28 days. At each time-point, the pellets were harvested for histological (hematoxylin and eosin (H&E)), histochemical (Alcian-Blue) and immunohistochemical analysis (Type I, II, and X collagen, aggrecan, SOX9, lubricin). After excluding CAG’s cytotoxicity (MTT Assay), improved cell condensation, higher glycosaminoglycans (sGAG) content, and increased cell proliferation have been detected in CAG–CM pellets until 28 days of culture. Overall, CAG improved the chondrogenic differentiation of hAMSCs, maintaining stable the active chondrocyte phenotype in up to 28 days of 3D in vitro chondrogenic culture. It is proposed that CAG might have a beneficial impact on cartilage regeneration approaches.

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