Application of fermentation for isoflavone extraction from soy molasses

2017 ◽  
Author(s):  
K. C. Duru ◽  
E. G. Kovaleva ◽  
T. V. Glukhareva
Keyword(s):  
Soy Molasses

scholarly journals Whole Conversion of Soybean Molasses into Isomaltulose and Ethanol by Combining Enzymatic Hydrolysis and Successive Selective Fermentations

Biomolecules ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 353
Author(s):  
Zhi-Peng Wang ◽  
Lin-Lin Zhang ◽  
Song Liu ◽  
Xiao-Yan Liu ◽  
Xin-Jun Yu
Keyword(s):  
Low Cost ◽  
Rhizomucor Miehei ◽  
Pichia Stipitis ◽  
Raffinose Family Oligosaccharides ◽  
Alternative Substrate ◽  
Soy Molasses ◽  
High Production ◽  
Novel Strategy ◽  
High Production Cost ◽  
High Sucrose

Isomaltulose is mainly produced from sucrose by microbial fermentation, when the utilization of sucrose contributes a high production cost. To achieve a low-cost isomaltulose production, soy molasses was introduced as an alternative substrate. Firstly, α-galactosidase gene from Rhizomucor miehei was expressed in Yarrowia lipolytica, which then showed a galactosidase activity of 121.6 U/mL. Under the effects of the recombinant α-galactosidase, most of the raffinose-family oligosaccharides in soy molasses were hydrolyzed into sucrose. Then the soy molasses hydrolysate with high sucrose content (22.04%, w/w) was supplemented into the medium, with an isomaltulose production of 209.4 g/L, and the yield of 0.95 g/g. Finally, by virtue of the bioremoval process using Pichia stipitis, sugar byproducts in broth were transformed into ethanol at the end of fermentation, thus resulting in high isomaltulose purity (97.8%). The bioprocess employed in this study provides a novel strategy for low-cost and efficient isomaltulose production from soybean molasses.

Biosynthesis of Medium-chain-length Poly(hydroxyalkanoates) from Soy Molasses

2006 ◽  
Vol 28 (3) ◽  
pp. 157-162 ◽  
Author(s):  
Daniel K. Y. Solaiman ◽  
Richard D. Ashby ◽  
Arland T. Hotchkiss ◽  
Thomas A. Foglia
Keyword(s):  
Chain Length ◽  
Medium Chain ◽  
Medium Chain Length ◽  
Soy Molasses

Simplified soy molasses-based medium for reduced-cost production of sophorolipids by Candida bombicola

2007 ◽  
Vol 29 (9) ◽  
pp. 1341-1347 ◽  
Author(s):  
Daniel K. Y. Solaiman ◽  
Richard D. Ashby ◽  
Jonathan A. Zerkowski ◽  
Thomas A. Foglia
Keyword(s):  
Candida Bombicola ◽  
Soy Molasses

scholarly journals Whole Conversion of Agro-Industrial Wastes Rich in Galactose-Based Carbohydrates into Lipid Using Oleaginous Yeast Aureobasidium Namibiae

2021 ◽  
Author(s):  
Zhi-Peng Wang ◽  
Jing-Run Ye ◽  
Yan Ma ◽  
Xin-Yue Zhang ◽  
Hai-ying Wang ◽  
...  
Keyword(s):  
Oleaginous Yeast ◽  
Raw Materials ◽  
Industrial Wastes ◽  
Lactose Hydrolysis ◽  
Efficient Utilization ◽  
Oleaginous Yeasts ◽  
Soy Molasses ◽  
First Time ◽  
Hydrolysis Of ◽  
Direct Hydrolysis

Abstract BackgroundRaw materials composed of easily assimilated monosaccharides have been employed as carbon source for production of microbial lipids. Nevertheless, agro-industrial wastes rich in galactose-based carbohydrates have not been introduced as feedstocks for oleaginous yeasts. ResultsIn this study, Aureobasidium namibiae A12 was found to efficiently accumulate lipid from soy molasses and whey powder containing galactose-based carbohydrates, with lipid productions of 5.30 g/L and 5.23 g/L, respectively. Over 80% of the fatty acids was C16:0, C18:0, C18:1, and C18:2. All kinds of single sugar components in the two byproducts were readily converted into lipids, with yields ranging between 0.116 g/g and 0.138 g/g. Three α-galactosidases and five β-galactosidases in the strain were cloned and analyzed. β-galactosidase was responsible for lactose hydrolysis; sucrase and α-galactosidase both contributed to the efficient hydrolysis of raffinose and stachyose in a cooperation manner. ConclusionsThis is a new way to produce lipids from raw materials containing galactose-based carbohydrates. This finding revealed the significance of sucrase in the direct hydrolysis of galactose-based carbohydrates in raw materials for the first time and facilitated the understanding of the efficient utilization of galactose-based carbohydrates to manufacture lipid or other chemicals in bioprocess

Soy molasses as a fermentation substrate for the production of biosurfactant using Pseudomonas aeruginosa ATCC 10145

2017 ◽  
Vol 24 (22) ◽  
pp. 18699-18709 ◽  
Author(s):  
Marília Silva Rodrigues ◽  
Felipe Santos Moreira ◽  
Vicelma Luiz Cardoso ◽  
Miriam Maria de Resende
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Soy Molasses ◽  
Fermentation Substrate
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