scholarly journals Whole Conversion of Soybean Molasses into Isomaltulose and Ethanol by Combining Enzymatic Hydrolysis and Successive Selective Fermentations

Biomolecules ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 353
Author(s):  
Zhi-Peng Wang ◽  
Lin-Lin Zhang ◽  
Song Liu ◽  
Xiao-Yan Liu ◽  
Xin-Jun Yu

Isomaltulose is mainly produced from sucrose by microbial fermentation, when the utilization of sucrose contributes a high production cost. To achieve a low-cost isomaltulose production, soy molasses was introduced as an alternative substrate. Firstly, α-galactosidase gene from Rhizomucor miehei was expressed in Yarrowia lipolytica, which then showed a galactosidase activity of 121.6 U/mL. Under the effects of the recombinant α-galactosidase, most of the raffinose-family oligosaccharides in soy molasses were hydrolyzed into sucrose. Then the soy molasses hydrolysate with high sucrose content (22.04%, w/w) was supplemented into the medium, with an isomaltulose production of 209.4 g/L, and the yield of 0.95 g/g. Finally, by virtue of the bioremoval process using Pichia stipitis, sugar byproducts in broth were transformed into ethanol at the end of fermentation, thus resulting in high isomaltulose purity (97.8%). The bioprocess employed in this study provides a novel strategy for low-cost and efficient isomaltulose production from soybean molasses.

2009 ◽  
Vol 1219 ◽  
Author(s):  
Ryoji Mizuno ◽  
Hitomi Ichinose ◽  
Tomoko Maehara ◽  
Koji Takabatake

AbstractWorldwide attention has now focused on bioethanol production to combat global warming and to safeguard global energy. Lignocelluloses are expected to be utilized in future as fuel ethanol production because of competition between food and fuel production. One of the major problems in producing ethanol from lignocellulosic biomass is high production cost and consolidated bioprocessing (CBP) is gaining recognition as a potential breakthrough for low-cost biomass processing. Basidiomycetes appear suitable for use in CBP because they can achieve the both events of lignocellulose breakdown and ethanol fermentation. We are developing CBP bioethanol production by usingFlammulina velutipesfrom sorghums. It turns out the relationship between varietal characteristics of sorghums and ethanol conversion properties ofF. velutipes, and the direction should be performed in the future became clear.


Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3778
Author(s):  
Jiping Zhao ◽  
Ganqiao Ran ◽  
Mengmeng Xu ◽  
Xiaoyun Lu ◽  
Dan Tan

3,4-dihydroxyphenyl-L-alanine (L-DOPA) is a preferred drug for Parkinson’s disease, with an increasing demand worldwide that mainly relies on costly and environmentally problematic chemical synthesis. Yet, biological L-DOPA production is unfeasible at the industrial scale due to its low L-DOPA yield and high production cost. In this study, low-cost Halomonas bluephagenesis TD01 was engineered to produce tyrosinase TyrVs-immobilized polyhydroxyalkanoate (PHA) nanogranules in vivo, with the improved PHA content and increased immobilization efficiency of TyrVs accounting for 6.85% on the surface of PHA. A higher L-DOPA-forming monophenolase activity of 518.87 U/g PHA granules and an L-DOPA concentration of 974.36 mg/L in 3 h catalysis were achieved, compared to those of E. coli. Together with the result of L-DOPA production directly by cell lysates containing PHA-TyrVs nanogranules, our study demonstrated the robust and cost-effective production of L-DOPA by H. bluephagenesis, further contributing to its low-cost industrial production based on next-generation industrial biotechnology (NGIB).


2014 ◽  
Vol 24 (1-2) ◽  
pp. 191-201 ◽  
Author(s):  
S Sheheli ◽  
K Fatema ◽  
SM Haque

The study was conducted to investigate the existing status and practices of fish farming. A total of 100 farmers were interviewed by using a structured interview schedule from three villages (Porabari, Mathabari and Vabanipur) of Trishal upazila of Mymensingh district at their houses and/or farm sites during January to May 2013. Most of the farmers (89%) made profit from fish production. The study confirmed that most farmers have improved their socio-economic conditions through fish production which plays an important role in increasing income, food production and employment opportunities. Five major areas are identified to improve the existing fish farming situation, which are quality fry, credit facilities, low-cost quality feed, training, and marketing channel. The impact analysis of fish farming on livelihood of fish farmers shows that overall 64% fish farmers have increased overall livelihood from fish farming during the last four years (2010-2013). Access to micro-credit, provide good quality input such as fry, feed, vaccines, etc., market facilities, supply of improve technologies, and provide training all lead to increased fish production. The constraints index (CI) analysis shows that overall 74% fish farmers faced medium constraints for fish farming. A total of nine (9) main constraints identified hindering their fish farming, and major constraints are high production cost, lack of technical knowledge and inadequate supply of good quality fry etc.DOI: http://dx.doi.org/10.3329/pa.v24i1-2.19172 Progress. Agric. 24(1&2): 191 - 201, 2013


2016 ◽  
Vol 4 (01) ◽  
pp. 57-62 ◽  
Author(s):  
G. Mahitha ◽  
R. Jaya Madhuri

Bioplastic, Polyhydroxybutyrate (PHB) is well known for it’s environmental friendliness and complete decomposition into water and carbon dioxide by microorganisms. The main drawback of PHB commercialization is it’s high production cost which is 10 times higher than that of synthetic plastic. So, the present research work mainly focussed on the fermentative production of PHB by Bacillus amyloliquifaciens and Nocardiopsis potens using low cost raw materials like Molasses, wheat bran, rice bran, ragi bran, jambul seed powder, orange peel and whey as substrates. Bacillus amyloliquifaciens and Nocardiopsis potens gives maximum PHB 16.5 µg/ ml and 26.8 µg/ ml respectively in the medium containing molasses and wheat bran as a substrates. Further the functional groups of extracted PHB were confirmed by Fourier transform infrared spectroscopy.


2021 ◽  
pp. 107754632098820
Author(s):  
Bashir B Muhammad ◽  
Muhammad Bashir ◽  
Mukhtar F Hamza ◽  
Mustapha Abdulhadi ◽  
Muhammad A Shehu ◽  
...  

A chatter mark is a result of irregular vibration that affects the milling process, which results in poor surface finish, reduced work quality, machine impairment, and high production cost. This work presents an active feedback controller design using a new response matrix to suppress the free vibration in the milling process. The proposed controller considers feed rate, tooth passing frequency, and time-varying dynamic milling force coefficients. A milling experiment verifies the effect of the proposed method. The method provides a reliable way of tackling chatter vibration in an industrial process. The procedure is technically and economically beneficial.


Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3192 ◽  
Author(s):  
Dong Chen ◽  
Xiaodong Wang ◽  
Wenhui Ding ◽  
Wenbing Zou ◽  
Qiong Zhu ◽  
...  

Owing to their ultra-low thermal conductivity, silica aerogels are promising thermal insulators; however, their extensive application is limited by their high production cost. Thus, scientists have started to explore low-cost and easy preparation processes of silica aerogels. In this work, a low-cost method was proposed to prepare silica aerogels with industrial silica hydrosol and a subsequent ambient pressure drying (APD) process. Various surfactants (cationic, amphoteric, or anionic) were added to avoid solvent exchange and surface modification during the APD process. The effects of various surfactants on the microstructure, thermal conductivity, and thermal stability of the silica aerogels were studied. The results showed that the silica aerogels prepared with a cationic or anionic surfactant have better thermal stability than that prepared with an amphoteric surfactant. After being heated at 600 °C, the silica aerogel prepared with a cationic surfactant showed the highest specific surface area of 131 m2∙g−1 and the lowest thermal conductivity of 0.038 W∙m−1∙K−1. The obtained low-cost silica aerogel with low thermal conductivity could be widely applied as a thermal insulator for building and industrial energy-saving applications.


2021 ◽  
Vol 58 (4) ◽  
pp. 246-258
Author(s):  
Jayata Mawani ◽  
Jagruti Jadhav ◽  
Amit Pratap

Abstract Mannosylerythritol lipids are glycolipid biosurfactants with promising industrial applications. However, their commercial production is hindered due to its high production cost. The current study investigates the use of sweetwater, a by-product of the fat-splitting industry in combination with soybean oil for the production of mannosylerythritol lipids using Pseudozyma antarctica (MTCC 2706). The optimum sweetwater and soybean oil concentration of 22% and 7% (w/v) yielded 7.52 g L–1and 21.5 g L–1 mannosylerythritol lipids at shake flask and fermenter level respectively. The structure and functional groups of mannosylerythritol lipids were confirmed by fourier transform infrared (FTIR) spectroscopy, liquid chromatography-mass spectrometry (LC/MS) and 1H- and 13C-nuclear magnetic resonance (NMR) analysis. Surfactant properties, such as surface tension, critical micelle concentration, foaming and emulsification of mannosylerythritol lipids were also explored.


2019 ◽  
Vol 37 (1_suppl) ◽  
pp. 73-82 ◽  
Author(s):  
Marco Limburg ◽  
Jan Stockschläder ◽  
Peter Quicker

The increasing use of carbon fibre reinforced polymers requires suitable disposing and recycling options, the latter being especially attractive due to the high production cost of the material. Reclaiming the fibres from their polymer matrix however is not without challenges. Pyrolysis leads to a decay of the polymer matrix but may also leave solid carbon residues on the fibre. These residues prevent fibre sizing and thereby reuse in new materials. In state of the art, these residues are removed via thermal treatment in oxygen containing atmospheres. This however may damage the fibre’s tensile strength. Within the scope of this work, carbon dioxide and water vapour were used to remove the carbon residues. This aims to eliminate or at least minimize fibre damage. Improved quality of reclaimed fibres can make fibre reuse more desirable by enabling the production of high-quality recycling products. Still, even under ideal recycling conditions the fibres will shorten with every new life-cycle due to production-based blending. Fibre disposal pathways will therefore always also be necessary. The problems of thermal fibre disintegration are summarized in the second part of this article (Part 2: Energy recovery).


2018 ◽  
Vol 30 (3) ◽  
pp. 412-427 ◽  
Author(s):  
Yuhui Wei ◽  
Zhaowei Su ◽  
Huashan Lu ◽  
Xue Mei Ding

Purpose The purpose of this paper is to develop an efficient termination control strategy of air-vented dryer in term of energy saving, improving smoothness and reducing microscopic damage of fiber. Design/methodology/approach A simple, low cost termination control strategy is developed by testing the instantaneous humidity of exhaust air and then deducing the drying degree of fabric in process. The practicability evaluation of this novel strategy was investigated by using both experimental and mathematical approaches. The effect of termination control strategy on drying efficiency and fabric apparent properties were also discussed. Findings Termination control strategy significantly affects drying time, energy consumption, smoothness and microscopic of fiber. Specially, a novel termination control strategy that the combination of equilibrium moisture content of fabric in ambient environment and relative humidity of exhaust air in exhaust duct is workable and can save 25.2 percent of energy consumption, 26.7 percent of the drying time and improve 0.7 grade of the appearance smoothness, as well as significantly reduce the microscopic damage of fiber compare to the original control strategy of dryer. This indicates possible ways to minimize drying energy consumption and dryer damage by reducing unnecessary migrate out of the water from the clothes. Practical implications The paper is helpful in not only the development of new drying product but also the optimization of appearance smoothness of fabric after drying and reduce the microscopic damage of fiber. Originality/value A novel termination control strategy of dryer is applied to improve drying efficiency of dryer and reduce fabric damage.


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