scholarly journals Effects of the pulse width on the reactive species production and DNA damage in cancer cells exposed to atmospheric pressure microsecond-pulsed helium plasma jets

AIP Advances ◽  
2017 ◽  
Vol 7 (8) ◽  
pp. 085106 ◽  
Author(s):  
Hea Min Joh ◽  
Ji Ye Choi ◽  
Sun Ja Kim ◽  
Tae Hong Kang ◽  
T. H. Chung
Keyword(s):  
Dna Damage ◽  
Cancer Cells ◽  
Atmospheric Pressure ◽  
Pulse Width ◽  
Reactive Species ◽  
Plasma Jets ◽  
Helium Plasma ◽  
Species Production

scholarly journals Tailoring the Chemistry of Plasma-Activated Water Using a DC-Pulse-Driven Non-Thermal Atmospheric-Pressure Helium Plasma Jet

Plasma ◽  
2019 ◽  
Vol 2 (2) ◽  
pp. 127-137 ◽  
Author(s):  
Oh ◽  
Szili ◽  
Hatta ◽  
Ito ◽  
Shirafuji
Keyword(s):  
Hydrogen Peroxide ◽  
Atmospheric Pressure ◽  
Pulse Width ◽  
Plasma Jet ◽  
Plasma Jets ◽  
Helium Plasma ◽  
Uv Absorption Spectroscopy ◽  
Pulse Polarity ◽  
Plasma Activated Water

We investigate the use of a DC-pulse-driven non-thermal atmospheric-pressure He plasma jet in the regulation of hydrogen peroxide (H2O2), nitrite (NO2−), nitrate (NO3−), and oxygen (O2) in deionized (DI) water. The production of these molecules is measured by in situ UV absorption spectroscopy of the plasma-activated water (PAW). Variations in the pulse polarity and pulse width have a significant influence on the resultant PAW chemistry. However, the trends in the concentrations of H2O2, NO2−, NO3−, and O2 are variable, pointing to the possibility that changes in the pulse polarity and pulse width might influence other plasma variables that also impact on the PAW chemistry. Overall, the results presented in this study highlight the possibility of using DC-pulse-driven plasma jets to tailor the chemistry of PAW, which opens new opportunities for the future development of optimal PAW formulations across diverse applications ranging from agriculture to medicine.

DNA damage in oral cancer cells induced by nitrogen atmospheric pressure plasma jets

2013 ◽  
Vol 102 (23) ◽  
pp. 233703 ◽  
Author(s):  
Xu Han ◽  
Matej Klas ◽  
Yueying Liu ◽  
M. Sharon Stack ◽  
Sylwia Ptasinska
Keyword(s):  
Dna Damage ◽  
Oral Cancer ◽  
Cancer Cells ◽  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
Plasma Jets ◽  
Pressure Plasma ◽  
Oral Cancer Cells

scholarly journals Large-Scale Image Analysis for Investigating Spatio-Temporal Changes in Nuclear DNA Damage Caused by Nitrogen Atmospheric Pressure Plasma Jets

2020 ◽  
Vol 21 (11) ◽  
pp. 4127
Author(s):  
Xu Han ◽  
James Kapaldo ◽  
Yueying Liu ◽  
M. Sharon Stack ◽  
Elahe Alizadeh ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Dna Damage ◽  
Image Analysis ◽  
Cancer Cells ◽  
Atmospheric Pressure ◽  
Large Scale ◽  
Nuclear Dna ◽  
Atmospheric Pressure Plasma ◽  
Plasma Jet ◽  
Spatio Temporal

The effective clinical application of atmospheric pressure plasma jet (APPJ) treatments requires a well-founded methodology that can describe the interactions between the plasma jet and a treated sample and the temporal and spatial changes that result from the treatment. In this study, we developed a large-scale image analysis method to identify the cell-cycle stage and quantify damage to nuclear DNA in single cells. The method was then tested and used to examine spatio-temporal distributions of nuclear DNA damage in two cell lines from the same anatomic location, namely the oral cavity, after treatment with a nitrogen APPJ. One cell line was malignant, and the other, nonmalignant. The results showed that DNA damage in cancer cells was maximized at the plasma jet treatment region, where the APPJ directly contacted the sample, and declined radially outward. As incubation continued, DNA damage in cancer cells decreased slightly over the first 4 h before rapidly decreasing by approximately 60% at 8 h post-treatment. In nonmalignant cells, no damage was observed within 1 h after treatment, but damage was detected 2 h after treatment. Notably, the damage was 5-fold less than that detected in irradiated cancer cells. Moreover, examining damage with respect to the cell cycle showed that S phase cells were more susceptible to DNA damage than either G1 or G2 phase cells. The proposed methodology for large-scale image analysis is not limited to APPJ post-treatment applications and can be utilized to evaluate biological samples affected by any type of radiation, and, more so, the cell-cycle classification can be used on any cell type with any nuclear DNA staining.

Effects of nitrogen impurity on the atmospheric pressure helium plasma jets exposed to a nitrogen environment

2020 ◽  
Vol 27 (10) ◽  
pp. 103511
Author(s):  
YuanYuan Jiang ◽  
Yanhui Wang ◽  
Shiyuan Cong ◽  
Jiao Zhang ◽  
Dezhen Wang
Keyword(s):  
Atmospheric Pressure ◽  
Plasma Jets ◽  
Helium Plasma ◽  
Nitrogen Impurity

Two-dimensional numerical study of two counter-propagating helium plasma jets in air at atmospheric pressure

2014 ◽  
Vol 21 (6) ◽  
pp. 063505 ◽  
Author(s):  
Wen Yan ◽  
Fucheng Liu ◽  
Chaofeng Sang ◽  
Dezhen Wang
Keyword(s):  
Atmospheric Pressure ◽  
Numerical Study ◽  
Plasma Jets ◽  
Helium Plasma ◽  
Two Dimensional
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