scholarly journals GC-MS analysis of clove (Syzygium aromaticum) bud essential oil from Java and Manado

Author(s):  
B. Amelia ◽  
E. Saepudin ◽  
A. H. Cahyana ◽  
D. U. Rahayu ◽  
A. S. Sulistyoningrum ◽  
...  
Separations ◽  
2020 ◽  
Vol 7 (2) ◽  
pp. 35
Author(s):  
Cristiane Marinho Uchôa Lopes ◽  
João Ricardhis Saturnino de Oliveira ◽  
Vanderlan Nogueira Holanda ◽  
Antonio Yony Felipe Rodrigues ◽  
Caíque Silveira Martins da Fonseca ◽  
...  

Clove (Syzygium aromaticum) is a spice widely used for its medical properties, though the species lacks scientific evidence regarding its toxicity and biologic effects. The aim of this study was the chemical identification by GC-MS analysis and evaluation of the hemolytic, anticoagulant, antidiarrheal and antipyretic activities of the essential oil from S. aromaticum (EOSa) in adult male mice. Essential oil was obtained by hydrodistillation and provided 9.8% v/w yield. GC-MS analyses allowed the identification of nine constituents, with eugenol (84.63%) as the majority. EOSa was diluted in several concentrations (0.005–2 mg/mL) for hemolytic assays, showing hemolytic activity above 20% in concentrations higher than 0.625 mg/mL. Different concentrations of EOSa induced a coagulation time 100% higher than control blood. 50 and 100 mg/kg of EOSa caused additional intestinal motility induced by castor oil by 90–100%. Fever, induced by Saccharomyces cerevisae 15% (s.c.), was controlled by 50 and 100 mg/kg EOSa (p.o.), effects similar to 100 mg/kg dypirone. Results showed that when used orally, EOSa may have a certain degree of toxicity in high dosages, but with antipyretic and intestinal motility properties.


BioResources ◽  
2019 ◽  
Vol 14 (2) ◽  
pp. 4489-4511 ◽  

Certain natural products extracted from different parts of medicinal and aromatic plants were examined for their antifungal activity against three plant pathogenic fungi, Fusarium oxysporum, Rhizoctonia solani, and Alternaria solani, and insecticidal activity against mosquito larvae (Culex pipiens). Acetone extract of Tectona grandis showed the highest antifungal activity against R. solani and A. solani with EC50 values of 118 and 294 μg/mL, respectively. The highest larvicidal activity was displayed by the essential oils of Ocimum basilicum and Eucalyptus gomphocephala with LC50 value of 22, and 30 mg/L, respectively. By gas chromatography–mass spectrometry (GC/MS) analysis 3-allylguaiacol (65.8%) and eugenol acetate (46.6%) were the main compounds in Syzygium aromaticum methanolic extract and essential oil, respectively. The main compound in T. grandis acetone extract was cyclohexylpentyl oxalate (8.7%); its water extract contained (E)-4,4-dimethyl-2-pentene (51.1%); E. gomphocephala branch oil contained p-cymene (28.8%); Euphorbia paralias leaf extract contained 1βH-romneine (26.3%); the seed extract contained α-linolenic acid, TMS (15.2%); Punica granatum extract contained furfural (32.1%); and O. basilicum essential oil contained estragole (65.9%). Thus, extracts from the tested plants can be used as natural biofungicides to manage diseases caused by F. oxysporum, R. solani, and A. solani. Additionally, these extracts show potential larvicide activities against mosquito larvae.


2020 ◽  
Vol 5 (2) ◽  
pp. 364-375
Author(s):  
Amraini Amelia ◽  
◽  
Nining Sugihartini ◽  
Hari Susanti ◽  

This review aims to determine the types of bases that can be used every day, which are effective and efficient as anti-inflammatory drugs. The research method used was to review the development of clove essential oil formulations that have been carried out using various concentrations of various types of bases including M / A type cream, A / M type cream, water soluble base, lotion, emulgel, hydrocarbons, hydrogels and absorbents. The recommended formulation is type M / A cream with a concentration of 5% clove flower essential oil. The types of bases studied were M / A cream, type A / M cream, water soluble base, lotion, emulgel, hydrocarbons, hydrogels and absorption properties which had good physical properties and did not irritate the skin of the test animals. This review refers to several national and international journals released in the last ten years, from 2010 to 2020.


2015 ◽  
Vol 5 (4) ◽  
pp. 268-272 ◽  
Author(s):  
Pramod Singh ◽  
Harish Andola ◽  
M.S.M. Rawat ◽  
Geeta Joshi nee Pant ◽  
J.S. Jangwan

2013 ◽  
Vol 7 (21) ◽  
pp. 2454-2463 ◽  
Author(s):  
Sessou Philippe ◽  
Farougou Souaiuml bou ◽  
Yegrave houeacute nou Boniface ◽  
Agniwo Bienvenu ◽  
Alitonou Guy ◽  
...  

Author(s):  
Tiago Soraggi Battagin ◽  
Mario Nicolas Caccalano ◽  
Guilherme Dilarri ◽  
Caio Felipe Cavicchia Zamuner ◽  
Natália Alleoni ◽  
...  

2020 ◽  
Vol 44 (1) ◽  
Author(s):  
E. L. Mejía-Argueta ◽  
J. G. Santillán-Benítez ◽  
M. M. Canales-Martinez ◽  
A. Mendoza-Medellín

Abstract Background To test the antimicrobial potential of clove essential oil that has been less investigated on antimicrobial-resistant organisms (extended-spectrum β-lactamase-ESBL-producing Escherichia coli), we collected 135 ESBL-producing Escherichia coli strains given that E. coli is the major organism increasingly isolated as a cause of complicated urinary and gastrointestinal tract infections, which remains an important cause of therapy failure with antibiotics for the medical sector. Then, in this study, we evaluated the relationship between the antibacterial potential activity of Syzygium aromaticum essential oil (EOSA) and the expression of antibiotic-resistant genes (SHV-2, TEM-20) in plasmidic DNA on ESBL-producing E. coli using RT-PCR technique. Results EOSA was obtained by hydrodistillation. Using Kirby-Baüer method, we found that EOSA presented a smaller media (mean = 15.59 mm) in comparison with chloramphenicol (mean = 17.73 mm). Thus, there were significant differences (p < 0.0001). Furthermore, EOSA had an antibacterial activity, particularly on ECB132 (MIC: 10.0 mg/mL and MBC: 80.0 mg/mL), and a bacteriostatic effect by bactericidal kinetic. We found that the expression of antibiotic-resistant gene blaTEM-20 was 23.52% (4/17 strains) and no expression of blaSHV-2. EOSA presented such as majority compounds (eugenol, caryophyllene) using the GC–MS technique. Conclusions Plant essential oils and their active ingredients have potentially high bioactivity against a different target (membranes, cytoplasm, genetic material). In this research, EOSA might become an important adjuvant against urinary and gastrointestinal diseases caused by ESBL-producing E. coli.


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