Development of PVA-alginate as a matrix for enzymatic decolorization of textile dye in bioreactor system

2017 ◽  
Author(s):  
Dede Heri Yuli Yanto ◽  
Syifa Zahara ◽  
Raden Permana Budi Laksana ◽  
Sita Heris Anita ◽  
Maulida Oktaviani ◽  
...  
Keyword(s):  
Textile Dye ◽  
Enzymatic Decolorization ◽  
Bioreactor System

scholarly journals Enzymatic Decolorization of Remazol Brilliant Blue Royal (RB 19) textile dye by White Rot Fungi

2019 ◽  
Vol 4 (1) ◽  
pp. 11 ◽  
Author(s):  
Hamisu Afiya ◽  
Erkurt Emrah Ahmet ◽  
M. Manjur Shah
Keyword(s):  
Enzyme Activity ◽  
Oxygen Demand ◽  
White Rot Fungi ◽  
Ligninolytic Enzymes ◽  
White Rot ◽  
Brilliant Blue ◽  
Textile Dye ◽  
Synthetic Dyes ◽  
Enzymatic Decolorization ◽  
Living Organisms

Synthetic dyes are widely used by different industries with over 7 ×105 metric tons produce globally each year. Dyes pose adverse effects including chemical oxygen demand, visual pollution, cytotoxicity, genotoxicity, mutagenicity and carcinogenicity on various types of living organisms. The versatile white rot fungi (basidiomycetes fungi) have developed specialized ligninolytic enzymes for reductive cleavage of dyes and xenobiotics. The present study optimized the decolorization of Remazol brilliant blue royal (RBBR) dye by enzymatic extracts of Coriolus versicolor and Pleurotusostreatus. Experiments were carried out by varying one parameter i.e. pH (2.5-6.5), temperature (30oC-60oC), enzyme activity (3.3U-20U), dye concentration (10mg/L-125mg/L) and time (0-480mins), while others constant to study its effects on decolorization of RBBR. From the results obtained, the optimum conditions for decolorization of RBBR by extracts of C. versicolor and P. ostreatus were pH 4.0, temperature of 300C, enzyme activity 20U, dye concentrations of 100mg/L and 50mg/L for C. versicolor and P. ostreatus respectively at the end of 480 minutes. At the optimized conditions, decolorizations for C. versicolor and P. ostreatus were 80.42% and 70.42% respectively. Highest laccase activity (19.50U) was recorded in C. versicolor compare to P. ostreatus (1.41U).  

Biomolecules encapsulated TiO 2 nano‐cubes using Tinospora cordifolia for photodegradation of a textile dye

2019 ◽  
Vol 14 (12) ◽  
pp. 1229-1232 ◽  
Author(s):  
Harpreet Kaur ◽  
Vikas Goyal ◽  
Jagpreet Singh ◽  
Sanjeev Kumar ◽  
Mohit Rawat
Keyword(s):  
Tinospora Cordifolia ◽  
Textile Dye

Human liver cell cultivation for long-term in vitro toxicity studies in a miniaturized multi-compartment 3D bioreactor system

2011 ◽  
Vol 49 (01) ◽  
Author(s):  
SA Hoffmann ◽  
M Lübberstedt ◽  
U Müller-Vieira ◽  
D Knobeloch ◽  
A Nüssler ◽  
...  
Keyword(s):  
Liver Cell ◽  
Human Liver ◽  
In Vitro Toxicity ◽  
Cell Cultivation ◽  
Toxicity Studies ◽  
Human Liver Cell ◽  
Bioreactor System

Enzymatic Textile Dyes Decolorization by In vitro and In silico Studies

2019 ◽  
Vol 13 (4) ◽  
pp. 268-276
Author(s):  
Sridevi Ayla ◽  
Monika Kallubai ◽  
Suvarnalatha Devi Pallipati ◽  
Golla Narasimha
Keyword(s):  
Molecular Docking ◽  
In Silico ◽  
Docking Studies ◽  
Textile Dyes ◽  
Crude Enzyme ◽  
Eastern Ghats ◽  
Textile Dye ◽  
Phanerochaete Sordida ◽  
Molecular Docking Studies ◽  
In Silico Studies

Background:Laccase, a multicopper oxidoreductase (EC: 1.10.3.2), is a widely used enzyme in bioremediation of textile dye effluents. Fungal Laccase is preferably used as a remediating agent in the treatment and transformation of toxic organic pollutants. In this study, crude laccase from a basidiomycetes fungus, Phanerochaete sordida, was able to decolorize azo, antroquinone and indigoid dyes. In addition, interactions between dyes and enzyme were analysed using molecular docking studies.Methods:In this work, a white rot basidiomycete’s fungus, Phanerochaete sordida, was selected from forest soil isolates of Eastern Ghats, and Tirumala and lignolytic enzymes production was assayed after 7 days of incubation. The crude enzyme was checked for decolourisation of various synthetic textile dyes (Vat Brown, Acid Blue, Indigo, Reactive Blue and Reactive Black). Molecular docking studies were done using Autodock-4.2 to understand the interactions between dyes and enzymes.Results:Highest decolourisation efficiency was achieved with the crude enzyme in case of vat brown whereas the lowest decolourisation efficiency was achieved in Reactive blue decolourisation. Similar results were observed in their binding affinity with lignin peroxidase of Phanerochaete chrysosporium through molecular docking approach.Conclusion:Thus, experimental results and subsequent in silico validation involving an advanced remediation approach would be useful to reduce time and cost in other similar experiments.

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