Application of a Halbach magnetic array for long-range cell and particle separations in biological samples

2016 ◽  
Vol 108 (21) ◽  
pp. 213702 ◽  
Author(s):  
Joo H. Kang ◽  
Harry Driscoll ◽  
Michael Super ◽  
Donald E. Ingber
Keyword(s):  
Long Range ◽  
Biological Samples ◽  
Range Cell ◽  
Particle Separations

scholarly journals Rectified directional sensing in long-range cell migration

2014 ◽  
Vol 5 (1) ◽  
Author(s):  
Akihiko Nakajima ◽  
Shuji Ishihara ◽  
Daisuke Imoto ◽  
Satoshi Sawai
Keyword(s):  
Cell Migration ◽  
Long Range ◽  
Range Cell ◽  
Range Cell Migration

scholarly journals A Critical-like Collective State Leads to Long-range Cell Communication in Dictyostelium discoideum Aggregation

2016 ◽  
Author(s):  
Giovanna De Palo ◽  
Darvin Yi ◽  
Robert G. Endres
Keyword(s):  
Single Cell ◽  
Dictyostelium Discoideum ◽  
Long Range ◽  
Early Development ◽  
Cell Communication ◽  
Multiscale Model ◽  
Wild Type ◽  
Social Amoeba ◽  
Range Cell ◽  
Cell Cell

AbstractThe transition from single-cell to multicellular behavior is important in early development but rarely studied. The starvation-induced aggregation of the social amoeba Dictyostelium discoideum into a multicellular slug is known to result from single-cell chemotaxis towards emitted pulses of cyclic adenosine monophosphate (cAMP). However, how exactly do transient short-range chemical gradients lead to coherent collective movement at a macroscopic scale? Here, we developed a multiscale model verified by quantitative microscopy to describe wide-ranging behaviors from chemotaxis and excitability of individual cells to aggregation of thousands of cells. To better understand the mechanism of long-range cell-cell communication and hence aggregation, we analyzed cell-cell correlations, showing evidence of self-organization at the onset of aggregation (as opposed to following a leader cell). Surprisingly, cell collectives, despite their finite size, show features of criticality known from phase transitions in physical systems. By comparing wild-type and mutant cells with impaired aggregation, we found the longest cellcell communication distance in wild-type cells, suggesting that criticality provides an adaptive advantage and optimally sized aggregates for the dispersal of spores.Author SummaryCells are often coupled to each other in cell collectives, such as aggregates during early development, tissues in the developed organism, and tumors in disease. How do cells communicate over macroscopic distances much larger than the typical cell-cell distance to decide how they should behave? Here, we developed a multiscale model of social amoeba, spanning behavior from individuals to thousands of cells. We show that local cell-cell coupling via secreted chemicals may be tuned to a critical value, resulting in emergent long-range communication and heightened sensitivity. Hence, these aggregates are remarkably similar to bacterial biofilms and neuronal networks, all communicating in a pulse-like fashion. Similar organizing principles may also aid our understanding of the remarkable robustness in cancer development.

scholarly journals The Surface Proteome of Adult Neural Stem Cells in Zebrafish Unveils Long-Range Cell-Cell Connections and Age-Related Changes in Responsiveness to IGF

2019 ◽  
Vol 12 (2) ◽  
pp. 258-273 ◽  
Author(s):  
Jara Obermann ◽  
Felicia Wagner ◽  
Anita Kociaj ◽  
Alessandro Zambusi ◽  
Jovica Ninkovic ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Long Range ◽  
Adult Neural Stem Cells ◽  
Age Related ◽  
Range Cell ◽  
Surface Proteome ◽  
Age Related Changes ◽  
Cell Cell

POSITIVE FEEDBACK-ASSISTED SHORT/LONG-RANGE CELL SIGNALINGS IN MAPK CASCADES

2009 ◽  
Vol 20 (11) ◽  
pp. 1769-1787
Author(s):  
YANBIN ZHANG ◽  
KENIAN CHEN ◽  
JUNWEI WANG ◽  
AIMIN CHEN ◽  
TIANSHOU ZHOU
Keyword(s):  
Long Range ◽  
Positive Feedback ◽  
Information Transfer ◽  
Spatial Information ◽  
Signal Propagation ◽  
Large Cell ◽  
Long Distance ◽  
Mapk Cascades ◽  
Range Cell ◽  
Living Organisms

In this paper, we study potentials of positive feedback in spatial phosphoprotein signal propagation. For this, we consider a signaling pathway of four-tiered protein kinase cascades with each tier involving single (de)phosphorylation reactions only. In the case of a small cell, we propose a short positive feedback for short-range cell signaling, which can generate bistability to facilitate the phosphoprotein signal propagation from the plasma membrane to the periphery of cell nucleus. In contrast, in the case of a large cell for which the long-range signaling cannot be achieved by the short feedback, we propose a long positive feedback, and find that it can facilitate the propagation of phosphoprotein signal over a long distance. These results imply that positive-feedback mechanisms would be employed by living organisms for spatial information transfer and cellular decision-making processing.

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