Enzyme-Free and Label-Free Ultrasensitive Electrochemical Detection of Human Immunodeficiency Virus DNA in Biological Samples Based on Long-Range Self-Assembled DNA Nanostructures

2012 ◽  
Vol 84 (19) ◽  
pp. 8277-8283 ◽  
Author(s):  
Xian Chen ◽  
Cheng-Yi Hong ◽  
Ya-Hui Lin ◽  
Jing-Hua Chen ◽  
Guo-Nan Chen ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Long Range ◽  
Electrochemical Detection ◽  
Biological Samples ◽  
Dna Nanostructures ◽  
Label Free ◽  
Immunodeficiency Virus ◽  
Self Assembled

Label-free and dual-amplified electrochemical detection of Hg2+ based on self-assembled DNA nanostructures and target-triggered exonuclease cleavage activity

2016 ◽  
Vol 40 (8) ◽  
pp. 6686-6691 ◽  
Author(s):  
Ting Bao ◽  
Wei Wen ◽  
Lei Shu ◽  
Xiuhua Zhang ◽  
Shengfu Wang
Keyword(s):  
Electrochemical Detection ◽  
Signal Amplification ◽  
Electrochemical Biosensor ◽  
Dna Nanostructures ◽  
Label Free ◽  
Target Recycling ◽  
Cleavage Activity ◽  
Self Assembled ◽  
Exo Iii

An electrochemical biosensor for Hg2+ detection via HCR and Hg2+-triggered Exo III-assisted target recycling for signal amplification.

Label-free and ultrasensitive electrochemiluminescence detection of microRNA based on long-range self-assembled DNA nanostructures

2013 ◽  
Vol 181 (7-8) ◽  
pp. 731-736 ◽  
Author(s):  
Ting Liu ◽  
Xian Chen ◽  
Cheng-Yi Hong ◽  
Xiao-Ping Xu ◽  
Huang-Hao Yang
Keyword(s):  
Long Range ◽  
Dna Nanostructures ◽  
Label Free ◽  
Self Assembled

DEVELOPMENT OF APPROACHES FOR DETECTION OF GENOME-INTEGRATED PROVIRAL DNA OF THE HUMAN IMMUNODEFICIENCY VIRUS (HIV-1) IN ULTRA LOW CONCENTRATIONS USING THE CRISPR/CAS SYSTEM

2020 ◽  
Author(s):  
M.A. Tyumentseva ◽  
◽  
A.I. Tyumentsev ◽  
V.G. Akimkin ◽  
◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Health Monitoring ◽  
Biological Samples ◽  
Proviral Dna ◽  
Guide Rnas ◽  
Ribonucleoprotein Complexes ◽  
Low Concentrations ◽  
Immunodeficiency Virus ◽  
Hiv 1

For the effective functioning of supervisory and health monitoring services, it is necessary to introduce modern molecular technologies into their practice. Therefore, the task of developing new effective methods for detecting pathogen, for example HIV, based on CRISPR/CAS genome editing systems, remains urgent. In the present work, guide RNAs and specific oligonucleotides were developed for preliminary amplification of highly conserved regions of the HIV-1 genome. The developed guide RNAs make it possible to detect single copies of HIV-1 proviral DNA in vitro as part of CRISPR/CAS ribonucleoprotein complexes in biological samples after preliminary amplification.

scholarly journals Dynamic Host Energetics and Cytoskeletal Proteomes in Human Immunodeficiency Virus Type 1-Infected Human Primary CD4 Cells: Analysis by Multiplexed Label-Free Mass Spectrometry

2009 ◽  
Vol 83 (18) ◽  
pp. 9283-9295 ◽  
Author(s):  
Eric Y. Chan ◽  
Jennifer N. Sutton ◽  
Jon M. Jacobs ◽  
Andrey Bondarenko ◽  
Richard D. Smith ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Human Immunodeficiency Virus ◽  
Virus Replication ◽  
Mass Spectrometry Analysis ◽  
Protein Abundance ◽  
Label Free ◽  
Cd4 Cells ◽  
Hiv Replication ◽  
Immunodeficiency Virus

ABSTRACT We report on a proteomic analysis of ex vivo human immunodeficiency virus (HIV) type 1 infection in human primary CD4 cells by shotgun liquid chromatography-tandem mass spectrometry analysis, revealing two distinct proteomic profiles at two phases of virus replication. Relative to mock-infected cells, 168 signature proteins exhibited abundance changes at the first sign of Gag p24 production (8 h postinfection [p.i.]) or the peak of virus replication (24 h p.i.); interestingly, most of the changes were exclusive to only one phase of virus replication. Based on characterization by functional ontology and known human-HIV protein interactions, we observed the enrichment for protein abundance increases pertaining to protein synthesis and nucleasomal reorganization amid an otherwise placid cellular proteome at the first sign of HIV replication. In contrast, we observed indications of decreased protein turnover, concomitant with heightened DNA repair activities and preludes to apoptosis, in the presence of robust virus replication. We also observed hints of disruptions in protein and small molecule trafficking. Our label-free proteomic strategy allowed us to perform multiplexed comparisons—we buttressed our detection specificity with the use of a reverse transcriptase inhibitor as a counterscreen, enabling highlighting of cellular protein abundance changes unique to robust virus replication as opposed to viral entry. In conjunction with complementary high-throughput screens for cellular partners of HIV, we put forth a model pinpointing specific rerouting of cellular biosynthetic, energetic, and trafficking pathways as HIV replication accelerates in human primary CD4 cells.

A new label-free fluorescent sensor for human immunodeficiency virus detection based on exonuclease III-assisted quadratic recycling amplification and DNA-scaffolded silver nanoclusters

The Analyst ◽  
2016 ◽  
Vol 141 (10) ◽  
pp. 2998-3003 ◽  
Author(s):  
Wen Yang ◽  
Jianniao Tian ◽  
Lijun Wang ◽  
Shui Fu ◽  
Hongyun Huang ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Fluorescent Sensor ◽  
Dna Detection ◽  
Virus Detection ◽  
Silver Nanoclusters ◽  
Label Free ◽  
Exonuclease Iii ◽  
Hiv Dna ◽  
Immunodeficiency Virus ◽  
Recycling Amplification

A label-free and sensitive fluorescence biosensing platform for HIV-DNA detection has been fabricated.

Label-free electrochemical detection of HepG2 tumor cells with a self-assembled DNA nanostructure-based aptasensor

2018 ◽  
Vol 268 ◽  
pp. 359-367 ◽  
Author(s):  
Duanping Sun ◽  
Jing Lu ◽  
Dabin Chen ◽  
Yunfei Jiang ◽  
Zhiru Wang ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Electrochemical Detection ◽  
Label Free ◽  
Dna Nanostructure ◽  
Self Assembled
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