scholarly journals Electrical lysis of cells for detergent-free droplet assays

2016 ◽  
Vol 10 (2) ◽  
pp. 024114 ◽  
Author(s):  
N. de Lange ◽  
T. M. Tran ◽  
A. R. Abate
Keyword(s):  
Electrical Lysis

Mechanism of Cell Lysis in Microfluidic Channel With Integrated Nanocomposite Electrodes

2013 ◽  
Author(s):  
Madhusmita Mishra ◽  
Anil Krishna Koduri ◽  
Aman Chandra ◽  
D. Roy Mahapatra ◽  
G. M. Hegde
Keyword(s):  
Electric Field ◽  
Microfluidic Channel ◽  
Cell Lysis ◽  
Bacterial Cells ◽  
Nano Composite ◽  
Time Resolved ◽  
Ac Electric Field ◽  
Cell Dispersion ◽  
Electrical Lysis ◽  
Nanocomposite Electrodes

This paper reports on the characterization of an integrated micro-fluidic platform for controlled electrical lysis of biological cells and subsequent extraction of intracellular biomolecules. The proposed methodology is capable of high throughput electrical cell lysis facilitated by nano-composite coated electrodes. The nano-composites are synthesized using Carbon Nanotube and ZnO nanorod dispersion in polymer. Bacterial cells are used to demonstrate the lysis performance of these nanocomposite electrodes. Investigation of electrical lysis in the microchannel is carried out under different parameters, one with continuous DC application and the other under DC biased AC electric field. Lysis in DC field is dependent on optimal field strength and governed by the cell type. By introducing the AC electrical field, the electrokinetics is controlled to prevent cell clogging in the micro-channel and ensure uniform cell dispersion and lysis. Lysis mechanism is analyzed with time-resolved fluorescence imaging which reveal the time scale of electrical lysis and explain the dynamic behavior of GFP-expressing E. coli cells under the electric field induced by nanocomposite electrodes. The DNA and protein samples extracted after lysis are compared with those obtained from a conventional chemical lysis method by using a UV–Visible spectroscopy and fluorimetry. The paper also focuses on the mechanistic understanding of the nano-composite coating material and the film thickness on the leakage charge densities which lead to differential lysis efficiency.

scholarly journals Electrophoretic Concentration and Electrical Lysis of Bacteria in a Microfluidic Device Using a Nanoporous Membrane

Micromachines ◽  
2017 ◽  
Vol 8 (2) ◽  
pp. 45 ◽  
Author(s):  
Md. Islam ◽  
Ali Shahid ◽  
Kacper Kuryllo ◽  
Yingfu Li ◽  
M. Deen ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Nanoporous Membrane ◽  
Electrophoretic Concentration ◽  
Electrical Lysis

Micromotor-based localized electroporation and gene transfection of mammalian cells

2021 ◽  
Vol 118 (38) ◽  
pp. e2106353118
Author(s):  
Yue Wu ◽  
Afu Fu ◽  
Gilad Yossifon
Keyword(s):  
Mammalian Cells ◽  
Single Cells ◽  
Gene Transfection ◽  
Target Cells ◽  
Suspension Cells ◽  
Janus Particle ◽  
Reversible Electroporation ◽  
Electrical Lysis ◽  
Extended Exposure ◽  
Biology Research

Herein, we studied localized electroporation and gene transfection of mammalian cells using a metallodielectric hybrid micromotor that is magnetically and electrically powered. Much like nanochannel-based, local electroporation of single cells, the presented micromotor was expected to increase reversible electroporation yield, relative to standard electroporation, as only a small portion of the cell’s membrane (in contact with the micromotor) is affected. In contrast to methods in which the entire membrane of all cells within the sample are electroporated, the presented micromotor can perform, via magnetic steering, localized, spatially precise electroporation of the target cells that it traps and transports. In order to minimize nonselective electrical lysis of all cells within the chamber, resulting from extended exposure to an electrical field, magnetic propulsion was used to approach the immediate vicinity of the targeted cell, after which short-duration, electric-driven propulsion was activated to enable contact with the cell, followed by electroporation. In addition to local injection of fluorescent dye molecules, we demonstrated that the micromotor can enhance the introduction of plasmids into the suspension cells because of the dielectrophoretic accumulation of the plasmids in between the Janus particle and the attached cell prior to the electroporation step. Here, we chose a different strategy involving the simultaneous operation of many micromotors that are self-propelling, without external steering, and pair with cells in an autonomic manner. The locally electroporated suspension cells that are considered to be very difficult to transfect were shown to express the transfected gene, which is of significant importance for molecular biology research.

scholarly journals Integration of marker-free selection of single cells at a wireless electrode array with parallel fluidic isolation and electrical lysis

2019 ◽  
Vol 10 (5) ◽  
pp. 1506-1513 ◽  
Author(s):  
Min Li ◽  
Robbyn K. Anand
Keyword(s):  
Single Cell ◽  
Single Cells ◽  
Electrode Array ◽  
Cell Capture ◽  
Bipolar Electrodes ◽  
Manual Intervention ◽  
Electrical Lysis ◽  
Marker Free ◽  
Selection Of

We present integration of selective single-cell capture at an array of wireless electrodes (bipolar electrodes, BPEs) with transfer into chambers, reagent exchange, fluidic isolation and rapid electrical lysis in a single platform, thus minimizing sample loss and manual intervention steps.

scholarly journals A Continuous Flow-through Microfluidic Device for Electrical Lysis of Cells

Micromachines ◽  
2019 ◽  
Vol 10 (4) ◽  
pp. 247 ◽  
Author(s):  
Ying-Jie Lo ◽  
U Lei
Keyword(s):  
Electric Field ◽  
Cross Flow ◽  
Electrode Array ◽  
Threshold Value ◽  
Lower Half ◽  
Rectangular Microchannel ◽  
Peak Voltage ◽  
Biomedical Systems ◽  
Electrical Lysis ◽  
Flow Through

In contrast to the delicate 3D electrodes in the literature, a simple flow-through device is proposed here for continuous and massive lysis of cells using electricity. The device is essentially a rectangular microchannel with a planar electrode array built on its bottom wall, actuated by alternating current (AC) voltages between neighboring electrodes, and can be incorporated easily into other biomedical systems. Human whole blood diluted 10 times with phosphate-buffered saline (about 6 × 108 cells per mL) was pumped through the device, and the cells were completely lysed within 7 s after the application of a 20 V peak-to-peak voltage at 1 MHz, up to 400 μL/hr. Electric field and Maxwell stress were calculated for assessing electrical lysis. Only the lower half-channel was exposed to an electric field exceeding the irreversible threshold value of cell electroporation (Eth2), suggesting that a cross flow, proposed here primarily as the electro-thermally induced flow, was responsible for bringing the cells in the upper half-channel downward to the lower half-channel. The Maxwell shear stress associated with Eth2 was one order of magnitude less than the threshold mechanical stresses for lysis, implying that an applied moderate mechanical stress could aid electrical lysis.

J0540101 Purity of cytoplasmic RNA extracted from single cells via electrical lysis and isotachophoresis

2015 ◽  
Vol 2015 (0) ◽  
pp. _J0540101--_J0540101-
Author(s):  
Shota HATA ◽  
Ryuji YOKOKAWA ◽  
Hidetoshi KOTERA ◽  
Hirofumi SHINTAKU
Keyword(s):  
Single Cells ◽  
Cytoplasmic Rna ◽  
Electrical Lysis

Fast Electrical Lysis of Cells for Capillary Electrophoresis

2003 ◽  
Vol 75 (15) ◽  
pp. 3688-3696 ◽  
Author(s):  
Futian Han ◽  
Yan Wang ◽  
Christopher E. Sims ◽  
Mark Bachman ◽  
Ruisheng Chang ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Electrical Lysis

Charge injection through nanocomposite electrode in microfluidic channel for electrical lysis of biological cells

2013 ◽  
Author(s):  
Madhusmita Mishra ◽  
Anil Krishna ◽  
Aman Chandra ◽  
B. M. Shenoy ◽  
G. M. Hegde ◽  
...  
Keyword(s):  
Microfluidic Channel ◽  
Charge Injection ◽  
Biological Cells ◽  
Electrical Lysis

scholarly journals Polymer Coatings in 3D-Printed Fluidic Device Channels for Improved Cellular Adherence Prior to Electrical Lysis

2015 ◽  
Vol 87 (12) ◽  
pp. 6335-6341 ◽  
Author(s):  
Bethany C. Gross ◽  
Kari B. Anderson ◽  
Jayda E. Meisel ◽  
Megan I. McNitt ◽  
Dana M. Spence
Keyword(s):  
Polymer Coatings ◽  
Electrical Lysis ◽  
Fluidic Device ◽  
3D Printed
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